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Protein Synthesis Reading
Protein Synthesis Reading

... protein, which in turn codes for a trait. Hence you hear it commonly referred to as the gene for baldness or the gene for blue eyes. Meanwhile, DNA is the chemical that genes and chromosomes are made of. DNA is called a nucleic acid because it was first found in the nucleus. We now know that DNA is ...
DNA EXTRACTION
DNA EXTRACTION

... (cytoplasm). Eukaryotes (such as animals, plants and fungi) store most of their DNA in a structure called nucleus. There are some DNA in mitochondria and chloroplasts as well. In the cell, DNA associates Figure 1. The stucture of DNA double helix. with some proteins, and together they form chromosom ...
BASIC INTRO TAXONOMY CELL THEORY PROKARYOTES
BASIC INTRO TAXONOMY CELL THEORY PROKARYOTES

... Live in extreme environments with high temperatures, and some produce methane. Vast difference in genetic and biochemical ...
Gene Section LYL1 (lymphoblastic leukemia derived sequence 1) in Oncology and Haematology
Gene Section LYL1 (lymphoblastic leukemia derived sequence 1) in Oncology and Haematology

... in the pathogenesis of T-ALL as well as myeloid malignancies (see below, disease implications). The LYL1 protein is a transcription factor (TF), structurally and functionally similar to another bHLH protein TAL1/SCL which is also implicated in T-ALL. Expression of both LYL1 and TAL1/SCL are regulate ...
The Masterof
The Masterof

... - This process of DNA from one cell entering another cell and becoming part of its DNA is called transformation ...
DNA and Genes student
DNA and Genes student

... Back to Copying DNA…. • Once mRNA is in the cytoplasm… Ribosomal RNA (rRNA) binds to the mRNA and uses the instructions to assemble the amino acids in the ...
SelfAssessment 1 – Cells
SelfAssessment 1 – Cells

... naturally in either sexual or asexual reproduction. State that genetic information can be passed from one bacterial cell to another bacterial cell via plasmids (small circular pieces of DNA). State that genetic material of viruses can be inserted into other organisms genetic material. State that gen ...
71071_Protein_synthesis
71071_Protein_synthesis

... • This process is called transcription, because the DNA transcribes “copies” ...
DNA: Transcription & Translation
DNA: Transcription & Translation

Protein Synthesis
Protein Synthesis

... – copies DNA in the nucleus and carries the info to the ribosomes (in cytoplasm) Ribosomal RNA (rRNA): – makes up a large part of the ribosome; reads and decodes mRNA Transfer RNA (tRNA): – carries amino acids to the ribosome where they are joined to form proteins ...
Slide 1
Slide 1

... small ribosomal subunit, the other larger ribosomal subunit binds as well, forming a complete ribosome  during translation, the mRNA threads through the ribosome three nucleotides at a time  a new tRNA holding an amino acid to be added enters the ribosome at the A site ...
Human Proteome advertising miniposter (PDF)
Human Proteome advertising miniposter (PDF)

... viruses. The antibody recognizes a unique part of the foreign target, the antigen. The unique properties of antibodies are used in a wide range of therapeutic and research applications. This poster describes some of the most common techniques. ...
Surface-activated Dynabeads
Surface-activated Dynabeads

... groups are available for coupling? The orientation of the active site of the ligand should also be taken into consideration. Hydrophobic beads facilitate interactions with hydrophobic parts of a protein, while hydrophilic beads are better suited when an interaction with hydrophilic parts of the prot ...


... A network of interconnected membranes forming channels within the cell. Covered with ribosomes (causing the "rough" appearance) which are in the process of synthesizing proteins for secretion or localization in membranes. (transport network) Ribosomes Making Proteins (01:54) Protein and RNA complex ...
Cytochrome P450 Proteins
Cytochrome P450 Proteins

... Assay selectivity, and sensitivity; Different technical expertise and equipment are needed for mRNA or Western Blotting assessment ...
01 Introduction - U of L Class Index
01 Introduction - U of L Class Index

... Thousands of newly identified species are added to the list each year. Estimates for total diversity of life range from about 10 mln to 100 mln species. ...
Recreating the Traumatized Muscle Microenvironment
Recreating the Traumatized Muscle Microenvironment

... 8 days in media containing 10% FBS with or without 20 ng/mL TGF-β1, with media changes on days 3 and 5. Real Time RT-PCR: RNA was isolated using Trizol phase separation and prepared for use in a 96-well osteogenesis pathway-specific RT2PCR array (SABiosciences). RESULTS A characteristic microscale c ...
Unit #3 Retake Ticket Unit 3 Retake Ticket
Unit #3 Retake Ticket Unit 3 Retake Ticket

... ______, and ______ have specific roles in this process. Structure B/G, known as __________, is important because it carries the DNA message from the (A)_____________ to the _______________. There, the (G) _________ attaches to the surface of (C) ___________, which is made partly of the second type o ...
bacteriophage
bacteriophage

DNA, RNA, and Protein Synthesis Notes 2006
DNA, RNA, and Protein Synthesis Notes 2006

... cytosine, guanine, and uracil c. single stranded molecule as opposed to double stranded B. Types of RNA 1. messenger RNA (mRNA) – carries information from the DNA to the ribosomes. 2. ribosomal RNA (rRNA) - type of RNA that makes up the major part of the ribosome. 3. transfer RNA (tRNA) – type of RN ...
EOCT REVIEW STUDY GUIDE
EOCT REVIEW STUDY GUIDE

... In animal cells, if water flows in unchecked, the cell will swell and burst. An example of this would be a red blood cell bursting when placed in fresh water. In a HYPERTONIC solution, cells can shrivel up because more water flows out of the cell than into it. OTHER MEANS OF TRANSPORT Facilitated di ...
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100

... These lack transport vessels and must absorb water by diffusion From the air. Their flagellated sperm must swim through water To fertilize an egg. They are tiny. ...
asdfs - The Wesley School
asdfs - The Wesley School

... Phase of the cell cycle where cells spend most of their time. They grow bigger and they do their job as body cells. G1 List the phases of mitosis in order starting with interphase Interphase, prophase, metaphase, ...
8.5 Translation
8.5 Translation

Chapter Five
Chapter Five

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Cell-penetrating peptide



Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.
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