High-resolution mapping of protein sequence
... WW domain, including both of the conserved tryptophan residues, and encompass the binding interface (Fig. 1a). We acquired sequencing data for the input variant library as well as after three and six rounds of selection, with an average of 10.7 million raw reads per library (Supplementary Table 1). ...
... WW domain, including both of the conserved tryptophan residues, and encompass the binding interface (Fig. 1a). We acquired sequencing data for the input variant library as well as after three and six rounds of selection, with an average of 10.7 million raw reads per library (Supplementary Table 1). ...
Defining characteristics of Tn5 Transposase non
... While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of nonspecific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest ...
... While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of nonspecific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest ...
Non-Type 1 Cystinuria Marker DNA Test for Mastiff
... Newfoundlands, is simply inherited as an autosomal recessive trait caused by a mutation in a gene that is known to cause cystinuria in people as well. This type of cystinuria is characterized by early ...
... Newfoundlands, is simply inherited as an autosomal recessive trait caused by a mutation in a gene that is known to cause cystinuria in people as well. This type of cystinuria is characterized by early ...
FORMATION, COMPOSITION AND SOME PROPERTIES OF
... %: 1.4 Pt, 22.4 Ti, 11.8 P, 64.4 O, i.e. the concentrations of platinum and titanium in dispersed particles (micrograines) are higher that those in the film. According to (6) conditions for formation of amorphous or crystal dispersed particles appear in the field of oversaturated solutions located n ...
... %: 1.4 Pt, 22.4 Ti, 11.8 P, 64.4 O, i.e. the concentrations of platinum and titanium in dispersed particles (micrograines) are higher that those in the film. According to (6) conditions for formation of amorphous or crystal dispersed particles appear in the field of oversaturated solutions located n ...
Metabolism and Biotransformation of Pesticides
... isozymes. Examples: aromatase, which catalyzes conversion of testosterone to estradiol. In the rat, it is present in the male testes and the female adrenal glands. As sperm matures, it Apparently requires pulses of estradiol, provided by pockets of aromatase in the testicular cells. Another example ...
... isozymes. Examples: aromatase, which catalyzes conversion of testosterone to estradiol. In the rat, it is present in the male testes and the female adrenal glands. As sperm matures, it Apparently requires pulses of estradiol, provided by pockets of aromatase in the testicular cells. Another example ...
lecture03_08
... This format contains a one line header followed by lines of sequence data. •Sequences in fasta formatted files are preceded by a line starting with a" >" symbol. •The first word on this line is the name of the sequence. •The remaining lines contain the sequence itself. •Blank lines in a FASTA file a ...
... This format contains a one line header followed by lines of sequence data. •Sequences in fasta formatted files are preceded by a line starting with a" >" symbol. •The first word on this line is the name of the sequence. •The remaining lines contain the sequence itself. •Blank lines in a FASTA file a ...
Putrescine oxidase of Micrococcus rubens : primary
... pH 7.5) containing 0.5 YONonidet P-40, 1 x Denhardt's solution and 50 pg salmon sperm DNA ml-' was carried out at 60 "C for 2 h. Hybridization was then done with the 32P-labelledprobes at 42 "C for 16 h in the same buffer. The nitrocellulose filters were washed three times in 2 x SSC (1 x SSC is 0.1 ...
... pH 7.5) containing 0.5 YONonidet P-40, 1 x Denhardt's solution and 50 pg salmon sperm DNA ml-' was carried out at 60 "C for 2 h. Hybridization was then done with the 32P-labelledprobes at 42 "C for 16 h in the same buffer. The nitrocellulose filters were washed three times in 2 x SSC (1 x SSC is 0.1 ...
Lab IV: Recombinant DNA Analysis
... Most of these mutations cause losses of function in general housekeeping genes. Their cumulative effect weakens the DH5 strain. As a result, DH5 cells do not survive well outside of our culture conditions and are not harmful to humans. But these mutations are also useful in three more specific way ...
... Most of these mutations cause losses of function in general housekeeping genes. Their cumulative effect weakens the DH5 strain. As a result, DH5 cells do not survive well outside of our culture conditions and are not harmful to humans. But these mutations are also useful in three more specific way ...
Genomic and Functional Approaches to Genetic Adaptation
... actually survive. Second, there should be variation in the ability of individuals to survive and reproduce, so that the fittest are those who survive, “the survival of the fittest”. Third, some of this variation should be heritable, allowing each generation to be better fitted to its ecological nich ...
... actually survive. Second, there should be variation in the ability of individuals to survive and reproduce, so that the fittest are those who survive, “the survival of the fittest”. Third, some of this variation should be heritable, allowing each generation to be better fitted to its ecological nich ...
S - www2
... In the case of serum albumin, the increase in radius is not accompanied by any change in the molecular weight, so the ratio of S/D remains constant as a function of pH. Also note that this information is not available from X-ray crystallography but requires these solution techniques. ...
... In the case of serum albumin, the increase in radius is not accompanied by any change in the molecular weight, so the ratio of S/D remains constant as a function of pH. Also note that this information is not available from X-ray crystallography but requires these solution techniques. ...
FX 8 - ACMG
... gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. Up to 44 copies of the CGG repeat within FMR-1 is considered normal. Premutation alleles are usually ...
... gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. Up to 44 copies of the CGG repeat within FMR-1 is considered normal. Premutation alleles are usually ...
Enzymes
... • Enzymes are mostly proteins (exception: ribozyme catalytic active RNA). • Some enzymes in addition to protein component contain also non-protein component. According to this we can divide enzymes into: – Simple enzymes contain only protein (pepsin, trypsin, ribonuclease). – Complex enzymes contain ...
... • Enzymes are mostly proteins (exception: ribozyme catalytic active RNA). • Some enzymes in addition to protein component contain also non-protein component. According to this we can divide enzymes into: – Simple enzymes contain only protein (pepsin, trypsin, ribonuclease). – Complex enzymes contain ...
1 - Free
... 14. name the compound which can carry the palmitate through the inner mitochondrial membrane and what kind of chemical bond formed between the two molecules, 15. the defect of which enzyme cause galagtosemia? 16. write with structure the pyruvate-kinase reaction. 17. list the substrate and products ...
... 14. name the compound which can carry the palmitate through the inner mitochondrial membrane and what kind of chemical bond formed between the two molecules, 15. the defect of which enzyme cause galagtosemia? 16. write with structure the pyruvate-kinase reaction. 17. list the substrate and products ...
Creatine kinase: The reactive cysteine is required for synergism but
... C278N. Thus, Cys278 probably provides a negative charge which is directly or indirectly involved in maximizing C K activity. Under near-optimal conditions in the reverse reaction, mutants C278G and C278S showed about an 1l-fold increase inKm(PCr),but only 1.7- and 2.8-fold reductions in V-, respecti ...
... C278N. Thus, Cys278 probably provides a negative charge which is directly or indirectly involved in maximizing C K activity. Under near-optimal conditions in the reverse reaction, mutants C278G and C278S showed about an 1l-fold increase inKm(PCr),but only 1.7- and 2.8-fold reductions in V-, respecti ...
The global repressor FliZ antagonizes gene
... EMSA with DNA fragments containing the promoters of mlrA and yciR. The mlrA gene encodes a MerR-like regulator essential for transcriptional activation of the central curli regulator CsgD (Supplementary Figure S1) which shows premature induction in post-exponential phase in a fliZ mutant (10). Simila ...
... EMSA with DNA fragments containing the promoters of mlrA and yciR. The mlrA gene encodes a MerR-like regulator essential for transcriptional activation of the central curli regulator CsgD (Supplementary Figure S1) which shows premature induction in post-exponential phase in a fliZ mutant (10). Simila ...
- Mendeley Data
... In the Schiff base compounds which are among the privileged ligands, the carbonyl group of ketone or aldehyde are replaced by an imine group due to simple one-pot condensation synthesis in an alcoholic solvent. For more than a century, metal complexes of the Schiff base ligands have been rigorously ...
... In the Schiff base compounds which are among the privileged ligands, the carbonyl group of ketone or aldehyde are replaced by an imine group due to simple one-pot condensation synthesis in an alcoholic solvent. For more than a century, metal complexes of the Schiff base ligands have been rigorously ...
Computer Storage of Sequences
... Comprehensive and expertly annotated protein sequence database. The primary sources of PSD data are sequences from GenBank/EMBL/DDBJ translations, published literature, and direct submission to PIR-International. ...
... Comprehensive and expertly annotated protein sequence database. The primary sources of PSD data are sequences from GenBank/EMBL/DDBJ translations, published literature, and direct submission to PIR-International. ...
Modifying the stereochemistry of an enzyme
... Directed evolution can provide a solution to this problem, and molecular biologists have successfully improved the properties of biocatalysts by altering activity (2), substrate specificity (3–5), and stability (6–9). There are two general ways in which the stereoisomeric product of an enzyme-cataly ...
... Directed evolution can provide a solution to this problem, and molecular biologists have successfully improved the properties of biocatalysts by altering activity (2), substrate specificity (3–5), and stability (6–9). There are two general ways in which the stereoisomeric product of an enzyme-cataly ...
Specialized Transduction
... high-frequency transducing phage lysates upon lysogenic induction. The chromosomes of these phage (called λ doc, for defective, one cohesive end) are produced by an aberration of the normal packaging mechanism in which one of the DNA ends arises by cos cleavage and the other arises by a less specif ...
... high-frequency transducing phage lysates upon lysogenic induction. The chromosomes of these phage (called λ doc, for defective, one cohesive end) are produced by an aberration of the normal packaging mechanism in which one of the DNA ends arises by cos cleavage and the other arises by a less specif ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.