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RNA Transcription
RNA Transcription

... for their arrangement in genomes. Since DNA is doublestranded, two different RNA molecules could in principle be transcribed from any gene, using each of the two DNA strands as a template. However a gene typically has only a single promoter, and because the nucleotide sequences of bacterial (as well ...
Genetic Engineering
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... with the segments of plant DNA, and treating with ligase. 3 Because the recombinant plasmids retain a gene for resistance to an antibiotic (R), bacterial cells that contain the plasmids are resistant to that ...
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Genes Are DNA
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... A nucleotide consists of a nucleoside linked to a phosphate group on either the 5′ or 3′ position of the (deoxy)ribose. Successive (deoxy)ribose residues of a polynucleotide chain are joined by a phosphate group ...
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... - Ribosomes are the machines that synthesize proteins based on the m-RNA template. Ribosomes are non-specific i.e. they are used in the synthesis of all proteins. iii. Transfer-RNA (t-RNA) - Transfers the appropriate amino-acid to a growing protein chain - There is one t-RNA for each amino-acid ...
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... The RNA molecules that carry copies of these instructions are known as messenger RNA (mRNA) because they serve as the messenger from DNA to the rest of the cell. ...
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... Therefore, the concentrator has two functions, first to allow low molecular weight inhibitory substances to pass into the filtrate while at the same time retaining the DNA above the membrane in a form that is recoverable. Factors such as membrane type, membrane orientation, and membrane area do not ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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