Lecture 3/30/15 by Dr. Katsunori Sugimoto
... a slightly increased risk of developing breast cancer. ...
... a slightly increased risk of developing breast cancer. ...
Organic Chem Biology
... peptide bond; two bound amino acids form a dipeptide, while many joined form a polypeptide. ...
... peptide bond; two bound amino acids form a dipeptide, while many joined form a polypeptide. ...
Introduction Biotechnology Recombinant DNA Genetic Engineering
... Screening for mRNA coding for protein of interest at different parts/timings of the metabolic pathway / developmental stages Southern-blot similarity (Electrophoresis + Hybridization with Radioactive probe) o Western Blot Screening for protein of interest at different parts/times of metabolic ...
... Screening for mRNA coding for protein of interest at different parts/timings of the metabolic pathway / developmental stages Southern-blot similarity (Electrophoresis + Hybridization with Radioactive probe) o Western Blot Screening for protein of interest at different parts/times of metabolic ...
KS4 - Contemporary Science Issues | Home
... Rosalind Franklin was born in 1920 and grew up to be a gifted scientist, gaining a first class degree from Cambridge in a time when few women became scientists. She was trained to make rational decisions based on hard scientific evidence. She worked in Paris, becoming expert in x-ray diffraction, an ...
... Rosalind Franklin was born in 1920 and grew up to be a gifted scientist, gaining a first class degree from Cambridge in a time when few women became scientists. She was trained to make rational decisions based on hard scientific evidence. She worked in Paris, becoming expert in x-ray diffraction, an ...
Document
... particularly how to determine it. They were inclined to follow the method of Pauling who had deduced the a-helical structure by building a model consistent with the x-ray patterns from fibrous proteins. Like proteins, DNA was built from similar units – the bases adenine (A) thymine (T) guanine (G) a ...
... particularly how to determine it. They were inclined to follow the method of Pauling who had deduced the a-helical structure by building a model consistent with the x-ray patterns from fibrous proteins. Like proteins, DNA was built from similar units – the bases adenine (A) thymine (T) guanine (G) a ...
103 topic summary
... Amino acids: structures and classification (nonpolar, polar, acidic and basic) D and L amino acids and Fischer projections Acid and base properties of amino acids: zwitterions, isoelectric point (pI) Structures of amino acids at various pH’s Electrophoresis: general methods and applications Peptide ...
... Amino acids: structures and classification (nonpolar, polar, acidic and basic) D and L amino acids and Fischer projections Acid and base properties of amino acids: zwitterions, isoelectric point (pI) Structures of amino acids at various pH’s Electrophoresis: general methods and applications Peptide ...
Strawberry DNA extraction:
... with the naked eye. The white fibers we will see in our preparation today are actually thousands of DNA strands wrapped around each other. An individual DNA strand is so small, it can only be imaged by the most sophisticated and specialized electron microscope. Scientists who study DNA and the gene ...
... with the naked eye. The white fibers we will see in our preparation today are actually thousands of DNA strands wrapped around each other. An individual DNA strand is so small, it can only be imaged by the most sophisticated and specialized electron microscope. Scientists who study DNA and the gene ...
Bacterial Genetics
... 2. Operon: the organization of a set of genes in a biochemical pathway. 3. Transcription of the gene is regulated directly by RNA polymerase and “repressors” or “inducers” . 4. The Ribosome bind to the mRNA while it is being transcribed from the DNA. ...
... 2. Operon: the organization of a set of genes in a biochemical pathway. 3. Transcription of the gene is regulated directly by RNA polymerase and “repressors” or “inducers” . 4. The Ribosome bind to the mRNA while it is being transcribed from the DNA. ...
Stem cell researchers uncover previously unknown patterns in DNA
... influences DNA methylation patterning throughout the genome and that DNA methyltransfereases (the enzymes that methylates DNA) preferentially target nucloesome-bound DNA," said Pellegrini, an associate professor of molecular, cell and developmental biology and an informatics expert. The work was ini ...
... influences DNA methylation patterning throughout the genome and that DNA methyltransfereases (the enzymes that methylates DNA) preferentially target nucloesome-bound DNA," said Pellegrini, an associate professor of molecular, cell and developmental biology and an informatics expert. The work was ini ...
Cloning Vector
... that can be cut by many restriction enzymes a method of selecting for bacteria (or yeast for YACs) containing a vector with foreign DNA; uually accomplished by selectable markers for drug resistance ...
... that can be cut by many restriction enzymes a method of selecting for bacteria (or yeast for YACs) containing a vector with foreign DNA; uually accomplished by selectable markers for drug resistance ...
File - Dr Hayley Siddons
... • Organisms are different because the proteins in their cells are different • The DNA provided the information about which amino acids make up proteins • It is the arrangement of the base pairs (A-T, C-G) which will determine the type of protein made • Sections of DNA which ‘code’ for a protein are ...
... • Organisms are different because the proteins in their cells are different • The DNA provided the information about which amino acids make up proteins • It is the arrangement of the base pairs (A-T, C-G) which will determine the type of protein made • Sections of DNA which ‘code’ for a protein are ...
Basic Chemistry and Biochemistry Unit Review Sheet File
... 11. Measurement of the hydrogen ion concentration of a solution may be given in terms of _________________. 12. Glucose is a __________________________________, maltose is a __________________________, and starch is a _________________________________. 13. The type of reaction by which proteins are ...
... 11. Measurement of the hydrogen ion concentration of a solution may be given in terms of _________________. 12. Glucose is a __________________________________, maltose is a __________________________, and starch is a _________________________________. 13. The type of reaction by which proteins are ...
Fig. 1.12
... NUCLEIC ACIDS CHARACTERISTICS Hydrophobic stacking interactions in which two or more bases are positioned with the planes of their rings parallel are stabilise the three-dimensional structure of the nucleic acids (water contact is minimised). Second ...
... NUCLEIC ACIDS CHARACTERISTICS Hydrophobic stacking interactions in which two or more bases are positioned with the planes of their rings parallel are stabilise the three-dimensional structure of the nucleic acids (water contact is minimised). Second ...
7.2 Nucleic acids
... DNA specimens isolated from different tissues of the same species have the same base composition. The base composition of DNA in a given species does not change with an organism’s age, nutritional state, or changing environment. In all cellular DNAs, regardless of the species, A = T y G = C ⇒ ...
... DNA specimens isolated from different tissues of the same species have the same base composition. The base composition of DNA in a given species does not change with an organism’s age, nutritional state, or changing environment. In all cellular DNAs, regardless of the species, A = T y G = C ⇒ ...
Control of Eukaryotic Gene Expression (Learning Objectives)
... sequences (proximal and distal elements) 6. Compare and contrast pre and post transcriptional and translational controls of gene expression 7. Explain interference RNA and its role play in post-transcriptional and translational regulation of gene expression 8. Define ubiquitin and proteosome and exp ...
... sequences (proximal and distal elements) 6. Compare and contrast pre and post transcriptional and translational controls of gene expression 7. Explain interference RNA and its role play in post-transcriptional and translational regulation of gene expression 8. Define ubiquitin and proteosome and exp ...
DNA Technology
... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
Final Exam 2012 - Med Study Group
... the inhibitor molecule may be chemically unrelated to the substrate. 35. If an enzyme solution is saturated with substrate, the most effective way to obtain an even faster yield of products is to • add more of the enzyme. • heat the solution to 90°C. • add more substrate. • add an allosteric inhibit ...
... the inhibitor molecule may be chemically unrelated to the substrate. 35. If an enzyme solution is saturated with substrate, the most effective way to obtain an even faster yield of products is to • add more of the enzyme. • heat the solution to 90°C. • add more substrate. • add an allosteric inhibit ...
nucleic acids 3115
... DNA divides, separates, and attracts new nucleotides to replace the strand that split away. Interesting Scientific Fact: DNA and RNA work together to make protein. Protein, as you recall, is the type of molecule of which most living things are made. Here is how it works. The first part of the proces ...
... DNA divides, separates, and attracts new nucleotides to replace the strand that split away. Interesting Scientific Fact: DNA and RNA work together to make protein. Protein, as you recall, is the type of molecule of which most living things are made. Here is how it works. The first part of the proces ...
RNA polymerase
... indicate the same amino acids because of redundancy in the genetic code. • Other changes lead to switches from one amino acid to another with similar properties. • Still other mutations may occur in a region where the exact amino acid sequence is not essential for function. Copyright © 2002 Pearson ...
... indicate the same amino acids because of redundancy in the genetic code. • Other changes lead to switches from one amino acid to another with similar properties. • Still other mutations may occur in a region where the exact amino acid sequence is not essential for function. Copyright © 2002 Pearson ...
Slide 1
... • In this case, blood types reveal that 11 is also not the father of 21. 21 and 25 share the same mother as their siblings but assuming he is the same person for both, who is their father? • Here is some help…. – 25 has the disease. The disease is dominant so the father must also have it. – Also, 21 ...
... • In this case, blood types reveal that 11 is also not the father of 21. 21 and 25 share the same mother as their siblings but assuming he is the same person for both, who is their father? • Here is some help…. – 25 has the disease. The disease is dominant so the father must also have it. – Also, 21 ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.