DNA Structure & Function
... Erwin Chargaff: Adenine and thymine are always present in equal amounts, and cytosione & guanine are always present in equal amounts within a molecule of DNA Rosalind Franklin & Wilson: used X-ray crystallography to image DNA Watson & Crick: discovered that DNA is in the form of a double helix and t ...
... Erwin Chargaff: Adenine and thymine are always present in equal amounts, and cytosione & guanine are always present in equal amounts within a molecule of DNA Rosalind Franklin & Wilson: used X-ray crystallography to image DNA Watson & Crick: discovered that DNA is in the form of a double helix and t ...
Homologous chromosomes
... – Several regions of DNA are used to make a DNA fingerprint to make it more likely the fingerprint is unique. – Used in crime scenes, paternity tests, etc. – Compare banding patterns to make a match ...
... – Several regions of DNA are used to make a DNA fingerprint to make it more likely the fingerprint is unique. – Used in crime scenes, paternity tests, etc. – Compare banding patterns to make a match ...
Blank notes - local.brookings.k12.sd.us
... suggested that genes coded for enzymes each disease (phenotype) is caused by non-functional gene product ...
... suggested that genes coded for enzymes each disease (phenotype) is caused by non-functional gene product ...
Introduction to Biology
... enzymatic ingredients necessary for RNA transcription and translation. The synthetic virus was able to successfully replicate itself from this mixture.” ...
... enzymatic ingredients necessary for RNA transcription and translation. The synthetic virus was able to successfully replicate itself from this mixture.” ...
DNA, RNA, Protein Synthesis
... tRNA molecules • Each tRNA molecule has an anticodon that is complementary to a codon on mRNA coding for a certain amino acid (so most amino acids have more than one tRNA that will code for them) • The tRNA will then retrieve that amino acid and bring it to the ribosome for protein assembly ...
... tRNA molecules • Each tRNA molecule has an anticodon that is complementary to a codon on mRNA coding for a certain amino acid (so most amino acids have more than one tRNA that will code for them) • The tRNA will then retrieve that amino acid and bring it to the ribosome for protein assembly ...
MLPA Assay using GSP Kit
... by GeneFix™Saliva Preparation Kit on MLPA assay. Background MLPA (Multiplex ligation-dependent probe amplification) is a DNA-based technique developed by Schouten et al., for the detection of duplications and deletions of whole genes and individual exons. It is now widely used in both research and d ...
... by GeneFix™Saliva Preparation Kit on MLPA assay. Background MLPA (Multiplex ligation-dependent probe amplification) is a DNA-based technique developed by Schouten et al., for the detection of duplications and deletions of whole genes and individual exons. It is now widely used in both research and d ...
Gene Expression/Mutations
... allows processed mRNA to leave and go to cytoplasm to ribosome to make protein ...
... allows processed mRNA to leave and go to cytoplasm to ribosome to make protein ...
ENZYMES: THE MAJESTIC MOLECULES OF LIFE Part
... There are distinguished, in the active centre, a contact site (anchor site) for binding a substrate, and a catalytic site at which the conversion of the bound substrate takes place. However, this functional differentiation is somewhat arbitrary, since the binding of a substrate at the contact site d ...
... There are distinguished, in the active centre, a contact site (anchor site) for binding a substrate, and a catalytic site at which the conversion of the bound substrate takes place. However, this functional differentiation is somewhat arbitrary, since the binding of a substrate at the contact site d ...
Teacher Guide - the BIOTECH Project
... Todd’s differences are due to recessive traits, if Frank and Maryann both were carriers of these recessive traits, therefore heterozygous, Todd could have received the recessive genes associated with the trait from each of his parents. This aspect of inheritance is luck of the draw. Brown hair, brow ...
... Todd’s differences are due to recessive traits, if Frank and Maryann both were carriers of these recessive traits, therefore heterozygous, Todd could have received the recessive genes associated with the trait from each of his parents. This aspect of inheritance is luck of the draw. Brown hair, brow ...
NATIONAL UNIVERSITY OF SINGAPORE DEPARTMENT OF BIOLOGICAL SCIENCES ADVANCED PLACEMENT TEST
... 30. Which statement(s) below is/are correct regarding DNA replication? i. In the lagging strand, many short pieces of DNA are made and this requires many RNA primers and DNA polIII. ii. RNA primers are removed by PolI, which then fills in the gaps with DNA. iii. DNA ligase covalently connects the Ok ...
... 30. Which statement(s) below is/are correct regarding DNA replication? i. In the lagging strand, many short pieces of DNA are made and this requires many RNA primers and DNA polIII. ii. RNA primers are removed by PolI, which then fills in the gaps with DNA. iii. DNA ligase covalently connects the Ok ...
Intro to Biotechnology
... the genes inside your body's cells to stop disease. • Genes that don't work properly can cause disease. • Gene therapy replaces a faulty gene or adds a new gene in an attempt to cure disease or improve your body's ability to fight disease. • Gene therapy holds promise for treating a wide range of di ...
... the genes inside your body's cells to stop disease. • Genes that don't work properly can cause disease. • Gene therapy replaces a faulty gene or adds a new gene in an attempt to cure disease or improve your body's ability to fight disease. • Gene therapy holds promise for treating a wide range of di ...
Chapter 17 Presentation
... mRNA is the “messenger” or vehicle that carries the genetic information from the DNA to the protein synthesizing machinery. RNA polymerase pries apart the DNA and joins RNA nucleotides together in the 5’-->3’ direction (adding, again, to the free 3’ end). RNA polymerase is just like DNA polymerase, ...
... mRNA is the “messenger” or vehicle that carries the genetic information from the DNA to the protein synthesizing machinery. RNA polymerase pries apart the DNA and joins RNA nucleotides together in the 5’-->3’ direction (adding, again, to the free 3’ end). RNA polymerase is just like DNA polymerase, ...
Comparison of DNA damage by subionized and ionized energy electron collisions and novel component separable nonthermal atmospheric plasma
... MS/MS and XPS to compare the yield of DNA damage and find out new types of DNA damage. 3. Results and discussion We confirm that LEEs can indirectly generate DNA damage through DEA resonant process and HEE can directly generate DNA damage through one electron ionization. Fig. 1 shows HPLC chromatogr ...
... MS/MS and XPS to compare the yield of DNA damage and find out new types of DNA damage. 3. Results and discussion We confirm that LEEs can indirectly generate DNA damage through DEA resonant process and HEE can directly generate DNA damage through one electron ionization. Fig. 1 shows HPLC chromatogr ...
Recombinant DNA Registration Form
... working with micro-organisms involved in this research project want it? ...
... working with micro-organisms involved in this research project want it? ...
DNA Banks for Endangered Animal Species
... return to the same beaches when nesting have relied on DNA restriction fragment length polymorphisms (RFLPs) (5). In the foreseeable future, DNA sequencing will be fully automated, and our descendants will be able rapidly to derive the sequence of any organism whose DNA has been appropriately collec ...
... return to the same beaches when nesting have relied on DNA restriction fragment length polymorphisms (RFLPs) (5). In the foreseeable future, DNA sequencing will be fully automated, and our descendants will be able rapidly to derive the sequence of any organism whose DNA has been appropriately collec ...
TOPICS FOR EXAMINATION II – Biology 1406
... What is biotechnology?. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? ...
... What is biotechnology?. What are restriction endonucleases? What is special about the way they cleave DNA? From which kind of microorganisms are all restriction endonucleases obtained? How will DNA fragments of different sizes migrate through agarose gels, and how will they sort themselves by size? ...
Overview of the Recombinant DNA technology- the plasmid vector pUC19
... with EcoR1, the chromosome without the extra EcoR1 site produces a larger fragment than the other one. This difference is recognised using a probe that hybridises within the region encompassed by two flanking EcoR1 sites present in both molecules. A probe represents a molecule able to bind very spec ...
... with EcoR1, the chromosome without the extra EcoR1 site produces a larger fragment than the other one. This difference is recognised using a probe that hybridises within the region encompassed by two flanking EcoR1 sites present in both molecules. A probe represents a molecule able to bind very spec ...
... iii) For the following statements, write the letter of the amino acid to which the statement best applies. Note, you may want to use the same amino acid more than once. If two (or more) are equally acceptable, write all of them. In some cases none of these may apply. If so, just write ’none’.(1 pt e ...
Packet #3
... 6. You have a plasmid with genes for tetracycline resistance and ampicillin resistance, as shown in the diagram 6a. In the middle of the tetracycline resistance gene is a target site for the restriction enzyme BamHI. Therefore, when you insert the gene of interest into this plasmid using the BAMHI ...
... 6. You have a plasmid with genes for tetracycline resistance and ampicillin resistance, as shown in the diagram 6a. In the middle of the tetracycline resistance gene is a target site for the restriction enzyme BamHI. Therefore, when you insert the gene of interest into this plasmid using the BAMHI ...
Document
... DNA Microarrays - “DNA chips” - allow rapid and simultaneous screening of thousands of genes DNA segments from known genes (up to hundreds of bp long) are amplified by PCR and placed on a solid surface using robotic devices that accurately deposit nanoliter amounts of DNA solution Thousands of such ...
... DNA Microarrays - “DNA chips” - allow rapid and simultaneous screening of thousands of genes DNA segments from known genes (up to hundreds of bp long) are amplified by PCR and placed on a solid surface using robotic devices that accurately deposit nanoliter amounts of DNA solution Thousands of such ...
Worksheet
... 7.1.A1 Rosalind Franklin’s and Maurice Wilkins’ investigation of DNA structure by X-ray diffraction. When X-rays are directed at a material some is scattered by the material. This scattering is known as diffraction. For X-ray diffraction to work well the material ideally should be crystallised so t ...
... 7.1.A1 Rosalind Franklin’s and Maurice Wilkins’ investigation of DNA structure by X-ray diffraction. When X-rays are directed at a material some is scattered by the material. This scattering is known as diffraction. For X-ray diffraction to work well the material ideally should be crystallised so t ...
Jan 19
... 1) Separate Proteins by polyacrylamide gel electrophoresis 2) transfer & fix to a membrane 3) probe with suitable antibody (or other probe) 4) determine # & sizes of detected bands ...
... 1) Separate Proteins by polyacrylamide gel electrophoresis 2) transfer & fix to a membrane 3) probe with suitable antibody (or other probe) 4) determine # & sizes of detected bands ...
First cells ppt The first cells ppt
... that fell in 1969 was found to contain 80 amino acids, some lipids, simple sugars and nitrogenous bases! ...
... that fell in 1969 was found to contain 80 amino acids, some lipids, simple sugars and nitrogenous bases! ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.