Leukaemia Section t(10;11)(q22;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... CXXC6 (CXXC finger 6) is also called LCX (leukemia-associated protein with a CXXC domain) or TET1. DNA/RNA 8497 bp representing the whole coding sequence. At least 12 exons. Contains 3 bipartite nuclear localization sites, 1 alpha helice coiled-coil region and 1 cysteine rich domain with high level ...
... CXXC6 (CXXC finger 6) is also called LCX (leukemia-associated protein with a CXXC domain) or TET1. DNA/RNA 8497 bp representing the whole coding sequence. At least 12 exons. Contains 3 bipartite nuclear localization sites, 1 alpha helice coiled-coil region and 1 cysteine rich domain with high level ...
doc BIOL 200 Notes up to Midterm
... CAP binds to CAP site when complexed with cAMP CAP-cAMP interacts with RNAP and greatly stimulates rate of transcription initiation o Sigma factors Recognize specific DNA sequences as promoters and recruit RNA polymerase σ70 is best known, recognizes TTGACA….TATAAT (consensus sequence) σ54 ...
... CAP binds to CAP site when complexed with cAMP CAP-cAMP interacts with RNAP and greatly stimulates rate of transcription initiation o Sigma factors Recognize specific DNA sequences as promoters and recruit RNA polymerase σ70 is best known, recognizes TTGACA….TATAAT (consensus sequence) σ54 ...
Catalytic Leuckart−Wallach-Type Reductive Amination of Ketones
... reaction is in general most useful and efficient when performed catalytically, rather than stoichiometrically, but during the past 100 years, only a few reports on the catalytic version of LW reaction have been made.4 This is apparently because the reported methods using Raney Ni or Co could not ove ...
... reaction is in general most useful and efficient when performed catalytically, rather than stoichiometrically, but during the past 100 years, only a few reports on the catalytic version of LW reaction have been made.4 This is apparently because the reported methods using Raney Ni or Co could not ove ...
Assay Quality Considerations
... Introduction of unwanted nucleic acids into specimen - the sensitivity of PCR techniques makes them vulnerable to contamination Repeated amplification of the same target sequence leads to accumulation of amplification products in the laboratory environment A typical PCR generates as many as 109 cop ...
... Introduction of unwanted nucleic acids into specimen - the sensitivity of PCR techniques makes them vulnerable to contamination Repeated amplification of the same target sequence leads to accumulation of amplification products in the laboratory environment A typical PCR generates as many as 109 cop ...
Bioinformatics (Warm Up + Cracking the Genetic Code)
... What is Bioinformatics? Exmpl: Human genome is a string of length ≃3.200.000.000 However, (parts of) this sequence must be interpreted to get a biological meaning. • Find out the sequence of genomes and what does it tell us? Which parts code for proteins or enzymes? • Predict structure of RNA or pro ...
... What is Bioinformatics? Exmpl: Human genome is a string of length ≃3.200.000.000 However, (parts of) this sequence must be interpreted to get a biological meaning. • Find out the sequence of genomes and what does it tell us? Which parts code for proteins or enzymes? • Predict structure of RNA or pro ...
Microbes from a Neanderthal Bone
... In 2006, a team working on sequencing Neanderthal genome published the first million nucleotides (letters) from the genome. Having this DNA information on hand, researchers noticed that only a small part of it was actually Neanderthal, and more than twice as much came from bacteria. Moreover nothing ...
... In 2006, a team working on sequencing Neanderthal genome published the first million nucleotides (letters) from the genome. Having this DNA information on hand, researchers noticed that only a small part of it was actually Neanderthal, and more than twice as much came from bacteria. Moreover nothing ...
Extracellular Enzymes Lab
... • Allows only specific substrates and cofactors to bind with the enzyme • Aligns the substrate with the reaction center of the enzyme • The 3D enzyme structure and catalytic activity can be lost by exposing the enzyme to high temperatures, salinity, pH, and other extremes. These extremes “denature” ...
... • Allows only specific substrates and cofactors to bind with the enzyme • Aligns the substrate with the reaction center of the enzyme • The 3D enzyme structure and catalytic activity can be lost by exposing the enzyme to high temperatures, salinity, pH, and other extremes. These extremes “denature” ...
Organization: The 6 Essential Elements
... The enzyme changes the substrate in some way i. It may help break the substrate apart. ii. It may hold two (or more) substrates together closely so the two parts interact. The enzyme and the substrate separate. The substrate (now changed) is called the product. ...
... The enzyme changes the substrate in some way i. It may help break the substrate apart. ii. It may hold two (or more) substrates together closely so the two parts interact. The enzyme and the substrate separate. The substrate (now changed) is called the product. ...
Extra Credit to replace the Survival of the Fittest Lab
... cells and do much of the work inside organisms. They also act as enzymes helping to control metabolic reactions in organisms. Amino acids contain two functional groups, the carboxyl group (-COOH) and the amino group (-NH2). ...
... cells and do much of the work inside organisms. They also act as enzymes helping to control metabolic reactions in organisms. Amino acids contain two functional groups, the carboxyl group (-COOH) and the amino group (-NH2). ...
Knox. The Gene Genie.
... as molecular pruning shears. The enzymes, called nucleases, create breaks at specific sites in the double-stranded DNA helix; a cell then repairs the break, sometimes incorporating new genetic material that a scientist has placed in the nucleus. When Doudna and Charpentier began collaborating, the m ...
... as molecular pruning shears. The enzymes, called nucleases, create breaks at specific sites in the double-stranded DNA helix; a cell then repairs the break, sometimes incorporating new genetic material that a scientist has placed in the nucleus. When Doudna and Charpentier began collaborating, the m ...
Epigenetic regulation of gene transcription. Publications
... nucleosome, which consists of DNA wrapped around an octamer of four core histone proteins (H2A, H2B, H3 and H4). Chromatin packages DNA within the cell and is repressive to any process which requires access to the DNA including DNA repair, replication, recombination and gene transcription. Understan ...
... nucleosome, which consists of DNA wrapped around an octamer of four core histone proteins (H2A, H2B, H3 and H4). Chromatin packages DNA within the cell and is repressive to any process which requires access to the DNA including DNA repair, replication, recombination and gene transcription. Understan ...
Translation
... to DNA template (copying genetic information from DNA). Synthesis of new RNA strand is catalyzed by RNA polymerase. [FIG.] During transcription, one strand of DNA double helix serves as a template (template strand) while the second strand is referred to as coding strand. ...
... to DNA template (copying genetic information from DNA). Synthesis of new RNA strand is catalyzed by RNA polymerase. [FIG.] During transcription, one strand of DNA double helix serves as a template (template strand) while the second strand is referred to as coding strand. ...
SCIENTIFIC BACKGROUND OF GENETICS A
... When one cell divides into two daughter cells, the DNA, all 46 chromosomes, must be replicated. The specificity of base pairing between A/T and C/G is essential for the synthesis of new DNA strands that are identical to the parental DNA. Each strand of DNA serves as a template for DNA synthesis. Syn ...
... When one cell divides into two daughter cells, the DNA, all 46 chromosomes, must be replicated. The specificity of base pairing between A/T and C/G is essential for the synthesis of new DNA strands that are identical to the parental DNA. Each strand of DNA serves as a template for DNA synthesis. Syn ...
Translation
... to DNA template (copying genetic information from DNA). Synthesis of new RNA strand is catalyzed by RNA polymerase. [FIG.] During transcription, one strand of DNA double helix serves as a template (template strand) while the second strand is referred to as coding strand. ...
... to DNA template (copying genetic information from DNA). Synthesis of new RNA strand is catalyzed by RNA polymerase. [FIG.] During transcription, one strand of DNA double helix serves as a template (template strand) while the second strand is referred to as coding strand. ...
Metabolism-Antibiotic Sensitivity
... In bacterial cells, the translation of mRNA into protein can be divided into three major phases: initiation, elongation, and termination of the peptide chain. Protein synthesis starts with the association of mRNA, a 30S ribosomal subunit, and formyl-methionyltransfer RNA (fMet-tRNA) to form a 30S in ...
... In bacterial cells, the translation of mRNA into protein can be divided into three major phases: initiation, elongation, and termination of the peptide chain. Protein synthesis starts with the association of mRNA, a 30S ribosomal subunit, and formyl-methionyltransfer RNA (fMet-tRNA) to form a 30S in ...
Translation
... to DNA template (copying genetic information from DNA). Synthesis of new RNA strand is catalyzed by RNA polymerase. [FIG.] During transcription, one strand of DNA double helix serves as a template (template strand) while the second strand is referred to as coding strand. ...
... to DNA template (copying genetic information from DNA). Synthesis of new RNA strand is catalyzed by RNA polymerase. [FIG.] During transcription, one strand of DNA double helix serves as a template (template strand) while the second strand is referred to as coding strand. ...
Chemistry In Your Life
... • This process is carried out by large structures called ribosomes which are built from several segments of rRNA and a group of ribosomal proteins. • Ribosomes contain sites where mRNA and two tRNA’s can bind. • There is at least one tRNA for each of the 20 amino acids. Each tRNA contains three base ...
... • This process is carried out by large structures called ribosomes which are built from several segments of rRNA and a group of ribosomal proteins. • Ribosomes contain sites where mRNA and two tRNA’s can bind. • There is at least one tRNA for each of the 20 amino acids. Each tRNA contains three base ...
guidelines
... Please use lab equipments (i.e. pipettes, centrifuge, vortex, thermoshaker) only which are placed in the archive DNA lab, do not take any equipments from and to other labs! These equipments are labelled accordingly. Disposable products (like ...
... Please use lab equipments (i.e. pipettes, centrifuge, vortex, thermoshaker) only which are placed in the archive DNA lab, do not take any equipments from and to other labs! These equipments are labelled accordingly. Disposable products (like ...
Activity #5b. Plasmid DNA Isolation, Restriction Enzyme Digestion
... The process by which free DNA is taken up by and is incorporated into an organism is called genetic transformation. An organism with such DNA is referred to as transgenic. Transgenic plants, animals, yeast and bacteria have been become critical tools for the pharmaceutical, agricultural, and biomedi ...
... The process by which free DNA is taken up by and is incorporated into an organism is called genetic transformation. An organism with such DNA is referred to as transgenic. Transgenic plants, animals, yeast and bacteria have been become critical tools for the pharmaceutical, agricultural, and biomedi ...
Plant collection protocol
... a) Herbarium voucher: At least two specimens of the same plant must be prepared. One will be kept at the University of Johannesburg Herbarium, and the second deposited to a main herbarium in the region where the plant was collected. b) Few leaves stored in silica gel: The silica gel is a hygroscopic ...
... a) Herbarium voucher: At least two specimens of the same plant must be prepared. One will be kept at the University of Johannesburg Herbarium, and the second deposited to a main herbarium in the region where the plant was collected. b) Few leaves stored in silica gel: The silica gel is a hygroscopic ...
Protein Synthesis - Napa Valley College
... There are different types of RNA: Messenger RNA (mRNA) – single strand, carries information for making a protein from the nucleus to the cytosol Transfer RNA (tRNA) – single strand, folds back on itself. Each tRNA carries one specific amino acid and brings it to the ribosome Ribosomal RNA (r ...
... There are different types of RNA: Messenger RNA (mRNA) – single strand, carries information for making a protein from the nucleus to the cytosol Transfer RNA (tRNA) – single strand, folds back on itself. Each tRNA carries one specific amino acid and brings it to the ribosome Ribosomal RNA (r ...
Developing a Low Cost Noninvasive Prenatal Diagnosis for Genetic
... Developing a Low Cost Noninvasive Prenatal Diagnosis for Genetic Disorders Abstract Objectives/Goals The object of this study is to develop a low cost, noninvasive prenatal diagnosis using bench-top semiconductor sequencing platform (SSP). SSP can thus replace current expensive diagnosis method such ...
... Developing a Low Cost Noninvasive Prenatal Diagnosis for Genetic Disorders Abstract Objectives/Goals The object of this study is to develop a low cost, noninvasive prenatal diagnosis using bench-top semiconductor sequencing platform (SSP). SSP can thus replace current expensive diagnosis method such ...
The MYB and BHLH Transcription Factor Families
... What are MYB family transcription factors? - Myeloblast MYB (came from first identified MYB, which was in an avian oncogene) - 3 Repeats: R1, R2, R3 - In Arabidopsis, almost all of the MYB proteins belong to the MYB-R2R3 class - Structure: Helix-turn-Helix: two alpha helices joined by a short str ...
... What are MYB family transcription factors? - Myeloblast MYB (came from first identified MYB, which was in an avian oncogene) - 3 Repeats: R1, R2, R3 - In Arabidopsis, almost all of the MYB proteins belong to the MYB-R2R3 class - Structure: Helix-turn-Helix: two alpha helices joined by a short str ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.