1 Biology 205 Exam 1 4/21/16 1. Geography quiz
... 7. A mutation in a particular enzyme causes the enzyme to form more hydrogen bonds with its substrate. What effect do you think this might have on the enzyme’s function? Start your answer by defining KM, Vmax and turnover number and then answer the question in terms of KM, Vmax and turnover number. ...
... 7. A mutation in a particular enzyme causes the enzyme to form more hydrogen bonds with its substrate. What effect do you think this might have on the enzyme’s function? Start your answer by defining KM, Vmax and turnover number and then answer the question in terms of KM, Vmax and turnover number. ...
Biology Review
... There are two types of nucleic acids: DNA (deoxyribonucleic acid) stores genetic information in the cell and organism-it replicates and gets transmitted to other cells when they divide and also when an organism reproduces. DNA provides directions for its own replication. DNA also directs synthes ...
... There are two types of nucleic acids: DNA (deoxyribonucleic acid) stores genetic information in the cell and organism-it replicates and gets transmitted to other cells when they divide and also when an organism reproduces. DNA provides directions for its own replication. DNA also directs synthes ...
GeneMATRIX Universal DNA/RNA/Protein Purification Kit
... Note 6: Add 10 µl β-mercaptoethanol (β-ME) per 1 ml buffer Lyse ALL before use. Lyse ALL is stable for 1 month after addition of β-ME. Note 7: Add 10 µl β-mercaptoethanol (β-ME) per 1 ml buffer DRP before use. Buffer DRP is stable for 1 month after addition of β-ME. Note 8: Add 25 µl β-mercaptoethan ...
... Note 6: Add 10 µl β-mercaptoethanol (β-ME) per 1 ml buffer Lyse ALL before use. Lyse ALL is stable for 1 month after addition of β-ME. Note 7: Add 10 µl β-mercaptoethanol (β-ME) per 1 ml buffer DRP before use. Buffer DRP is stable for 1 month after addition of β-ME. Note 8: Add 25 µl β-mercaptoethan ...
Overview of milestones in genetics and genetic variation Author
... organisms and this material should fulfil three requirements: Replication ability-It should be able to replicate so that parents can pass this material to the offspring Information carrier-It must carry necessary information for the animal development and functioning Prone to changes-It should ...
... organisms and this material should fulfil three requirements: Replication ability-It should be able to replicate so that parents can pass this material to the offspring Information carrier-It must carry necessary information for the animal development and functioning Prone to changes-It should ...
Evidence for Evolution: Evolutionary Biologist
... (mutations) between each organism. If two organisms are very distantly related, we would expect a great number of differences in their DNA since more time has passed to allow for more mutations to occur. With the advancement of DNA technology, scientists have been able to compare these ‘genetic docu ...
... (mutations) between each organism. If two organisms are very distantly related, we would expect a great number of differences in their DNA since more time has passed to allow for more mutations to occur. With the advancement of DNA technology, scientists have been able to compare these ‘genetic docu ...
Genetic Mapping with CAPS Markers
... phenotype) consists basically of testing the linkage with a number of previously mapped genes or “markers” that also provide a phenotype. Genetic maps are constructed based on the principle that the frequency of recombination between genes decreases as the distance between them decreases. The freque ...
... phenotype) consists basically of testing the linkage with a number of previously mapped genes or “markers” that also provide a phenotype. Genetic maps are constructed based on the principle that the frequency of recombination between genes decreases as the distance between them decreases. The freque ...
CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES
... structure of proteins. A polypeptide can have up to four levels of structure. The primary structure is the sequence of the amino acids. Secondary structure contains α-helices and β-pleated sheets in place by hydrogen bonding between amino acids along the backbone of the polypeptide chain. The tertia ...
... structure of proteins. A polypeptide can have up to four levels of structure. The primary structure is the sequence of the amino acids. Secondary structure contains α-helices and β-pleated sheets in place by hydrogen bonding between amino acids along the backbone of the polypeptide chain. The tertia ...
Lab 1 Artificial Selection The purpose of a particular investigation
... The roots of several onion bulbs were removed. Then the remaining severed root mass of onions bulbs were submerged in a beaker of distilled water, and others were submerged in a beaker of water seeped with weeping willow bark. It has been reported that a substance found in the bark of the weeping wi ...
... The roots of several onion bulbs were removed. Then the remaining severed root mass of onions bulbs were submerged in a beaker of distilled water, and others were submerged in a beaker of water seeped with weeping willow bark. It has been reported that a substance found in the bark of the weeping wi ...
Sample Exam 3
... b) hydrogen bonds between chains c) ionic bonds between chains d) interactions that are not well understood 5. Which of the following is not true of DNA profiling? a) RFLP’s are the result of differences in non-coding DNA between individuals. b) Sufficient DNA can be isolated from an envelope flap t ...
... b) hydrogen bonds between chains c) ionic bonds between chains d) interactions that are not well understood 5. Which of the following is not true of DNA profiling? a) RFLP’s are the result of differences in non-coding DNA between individuals. b) Sufficient DNA can be isolated from an envelope flap t ...
20 DetailLectOut 2012
... One basic cloning technique begins with the insertion of a “foreign” gene into a bacterial plasmid to produce a recombinant DNA molecule. The plasmid is returned to a bacterial cell, producing a recombinant bacterium, which reproduces to form a clone of genetically identical cells. Every time the ba ...
... One basic cloning technique begins with the insertion of a “foreign” gene into a bacterial plasmid to produce a recombinant DNA molecule. The plasmid is returned to a bacterial cell, producing a recombinant bacterium, which reproduces to form a clone of genetically identical cells. Every time the ba ...
A.P. Biology Summer Work: Worksheet
... organic compounds, so carbon is essential to life on Earth. Without carbon, life as we know it could not exist. Why is carbon so basic to life? The reason is carbon’s ability to form stable bonds with many elements, including itself. This property allows carbon to form a huge variety of very large a ...
... organic compounds, so carbon is essential to life on Earth. Without carbon, life as we know it could not exist. Why is carbon so basic to life? The reason is carbon’s ability to form stable bonds with many elements, including itself. This property allows carbon to form a huge variety of very large a ...
Advanced techniques yield new insights into ribosome selfassembly
... simulation of the protein and RNA interaction. Their analysis revealed that the S4 protein and the 16S ribosomal RNA were a surprisingly “dynamic duo,” Ha said. The protein constrained the RNA somewhat, but still allowed it to undulate and change its conformation. The team found that the S4 protein ...
... simulation of the protein and RNA interaction. Their analysis revealed that the S4 protein and the 16S ribosomal RNA were a surprisingly “dynamic duo,” Ha said. The protein constrained the RNA somewhat, but still allowed it to undulate and change its conformation. The team found that the S4 protein ...
Contribution of forensic genetics to the recovery of historic memory
... amelogenin analysis ratifies itself as a rapid and useful tool in those cases where checking sex composition of bone remains from a specific grave is needed to be contrasted with data recorded by Historic Register, or merely by local people memory. As for maternal lineages analysis, regions HVSI and ...
... amelogenin analysis ratifies itself as a rapid and useful tool in those cases where checking sex composition of bone remains from a specific grave is needed to be contrasted with data recorded by Historic Register, or merely by local people memory. As for maternal lineages analysis, regions HVSI and ...
Document
... • Amino acid sequences of similar proteins were compared. • If evolution has taken place, then species descended from a recent common ancestor should have fewer amino acid differences in proteins than do species that aren’t as closely related. This pattern does not hold true for all proteins. A ce ...
... • Amino acid sequences of similar proteins were compared. • If evolution has taken place, then species descended from a recent common ancestor should have fewer amino acid differences in proteins than do species that aren’t as closely related. This pattern does not hold true for all proteins. A ce ...
385 Genetic Transformation : a Retrospective Appreciation
... pathways, the mutation in the recipient being indicated by the cross, while the ‘ B’ region is concerned with capsular specificity. Note that in transformation, as in other forms of bacterial sexuality, the fragmentary nature of the genetic contribution of the donor demands at least two genetic exch ...
... pathways, the mutation in the recipient being indicated by the cross, while the ‘ B’ region is concerned with capsular specificity. Note that in transformation, as in other forms of bacterial sexuality, the fragmentary nature of the genetic contribution of the donor demands at least two genetic exch ...
2012 Regional Test - North Carolina Science Olympiad
... 22. The number, lengths and staining patterns of chromosomes depicted in this photo is called what? 23. Each number sits below two chromosomes that are the same size and shape. What are these pairs of chromosomes called with respect to each other? ...
... 22. The number, lengths and staining patterns of chromosomes depicted in this photo is called what? 23. Each number sits below two chromosomes that are the same size and shape. What are these pairs of chromosomes called with respect to each other? ...
manual Monarch DNA Gel Extraction Kit T1020S T1020L
... higher temperatures than the specified range of 37–55°C. • Incomplete elution during prep. Ensure the DNA Elution Buffer is delivered directly to the center of the column so that the matrix is completely covered and elution is efficient. Larger elution volumes and longer incubation times can increa ...
... higher temperatures than the specified range of 37–55°C. • Incomplete elution during prep. Ensure the DNA Elution Buffer is delivered directly to the center of the column so that the matrix is completely covered and elution is efficient. Larger elution volumes and longer incubation times can increa ...
Macromolecules
... All digested carbohydrates broken down to this for absorption in the small intestine. Only fuel used by the brain/nervous tissue ...
... All digested carbohydrates broken down to this for absorption in the small intestine. Only fuel used by the brain/nervous tissue ...
The Flow Cell: The Sequencer:
... The MPS core is equipped with the Illumina HiSeq 1000. It can be used for genome wide DNA sequencing, RNAseq, ChipSeq, methylation sequencing, metagenomics, exome sequencing, and small RNA and DNA sequencing to name a few. It is not designed to replace a Sanger (capillary) sequencer because each run ...
... The MPS core is equipped with the Illumina HiSeq 1000. It can be used for genome wide DNA sequencing, RNAseq, ChipSeq, methylation sequencing, metagenomics, exome sequencing, and small RNA and DNA sequencing to name a few. It is not designed to replace a Sanger (capillary) sequencer because each run ...
FischerSpr10
... We have found MBP1 to be toxic to bacteria, therefore traditional methods for obtaining the expression construct will not work. A new protocol is being used where S. cerevisiae is transformed directly with the vector construct and cultured on selective media. Vector is then isolated and PCR ampl ...
... We have found MBP1 to be toxic to bacteria, therefore traditional methods for obtaining the expression construct will not work. A new protocol is being used where S. cerevisiae is transformed directly with the vector construct and cultured on selective media. Vector is then isolated and PCR ampl ...
PDF version
... made by commercial genotyping labs. The NBCEC is a group of researchers at some of the land-grant universities who work in quantitative and molecular genetics. Validation is initiated when a commercial lab request the NBCEC validates their marker claim. The NBCEC organizes a resource population to t ...
... made by commercial genotyping labs. The NBCEC is a group of researchers at some of the land-grant universities who work in quantitative and molecular genetics. Validation is initiated when a commercial lab request the NBCEC validates their marker claim. The NBCEC organizes a resource population to t ...
for Genetic Testing
... destroys the middle Mstll recognition site. The father and mother each yield two bands on their Southern blots, because they each carry one normal and one mutant gene. • Affected son II-1 has only the larger band, because he has two copies of the mutant gene. Daughter II-2 shows only the smaller ban ...
... destroys the middle Mstll recognition site. The father and mother each yield two bands on their Southern blots, because they each carry one normal and one mutant gene. • Affected son II-1 has only the larger band, because he has two copies of the mutant gene. Daughter II-2 shows only the smaller ban ...
Activity: Invasion of the Snorks
... 1. Create the data charts in your lab book. Make sure to leave enough room to have all of the necessary information present. 2. Using the mRNA from the Snork, find the missing strand of DNA belonging to the Snork. From what we know about the Snorks, the base pairing rules are the same as us. 3. Code ...
... 1. Create the data charts in your lab book. Make sure to leave enough room to have all of the necessary information present. 2. Using the mRNA from the Snork, find the missing strand of DNA belonging to the Snork. From what we know about the Snorks, the base pairing rules are the same as us. 3. Code ...
Deoxyribozyme
_DNAzyme.png?width=300)
Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.