Agarose gel reagents and buffers - Scie-Plas
... in buffer to dissolve it. It will then gel upon cooling. Like acrylamide the pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels, making them suitable for the separation of much larger nucl ...
... in buffer to dissolve it. It will then gel upon cooling. Like acrylamide the pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels, making them suitable for the separation of much larger nucl ...
The DnaE polymerase from Deinococcus radiodurans features
... strength conditions or in the presence of manganese. Further assays were performed using a ssDNA and a dsDNA, whose recombination yields a DNA substrate. Surprisingly, αDr was found to be incapable of recombination-dependent DNA polymerase activity, whereas αEc was competent in this action. However, ...
... strength conditions or in the presence of manganese. Further assays were performed using a ssDNA and a dsDNA, whose recombination yields a DNA substrate. Surprisingly, αDr was found to be incapable of recombination-dependent DNA polymerase activity, whereas αEc was competent in this action. However, ...
Competiitve Speciation
... Schematic depiction of one possible realization of the coalescent process in a population with 18 haploid gametes. The eighteen alleles in our current sample are descended from only four alleles that were present in the populations ten generations ago. How far back in time do we have to go to find t ...
... Schematic depiction of one possible realization of the coalescent process in a population with 18 haploid gametes. The eighteen alleles in our current sample are descended from only four alleles that were present in the populations ten generations ago. How far back in time do we have to go to find t ...
The Structure and Topology of Protein Serine/Threonine
... within five conserved sequence motifs found in other enzymes, including the purple acid phosphatase, whose common function is to catalyze phosphoryl transfer reactions to water [12]. These observations suggest that PPP and purple acid phosphatases evolved by divergent evolution from an ancestral met ...
... within five conserved sequence motifs found in other enzymes, including the purple acid phosphatase, whose common function is to catalyze phosphoryl transfer reactions to water [12]. These observations suggest that PPP and purple acid phosphatases evolved by divergent evolution from an ancestral met ...
Quantifying the DNA Binding Properties of the Binuclear Ruthenium
... Various sources of funding from BSU have made all of this possible. I would not have been able to dedicate so much time to this project without it. The ATP Summer Grant funding helped greatly during the first summer of research and the countless semester grants provided by them helped as well. The B ...
... Various sources of funding from BSU have made all of this possible. I would not have been able to dedicate so much time to this project without it. The ATP Summer Grant funding helped greatly during the first summer of research and the countless semester grants provided by them helped as well. The B ...
Inquiry into Life Twelfth Edition
... 13.3 Chromatin Structure and Gene Activity • Histones, especially H1, have a repressive effect on gene activity in vitro • Two families of 5S rRNA genes studied are oocyte and somatic genes – Oocyte genes are expressed only in oocytes – Somatic genes are expressed both in oocytes and somatic cells ...
... 13.3 Chromatin Structure and Gene Activity • Histones, especially H1, have a repressive effect on gene activity in vitro • Two families of 5S rRNA genes studied are oocyte and somatic genes – Oocyte genes are expressed only in oocytes – Somatic genes are expressed both in oocytes and somatic cells ...
June 2016 Common exam
... it is single stranded and contains uracil it is single stranded and contains thymine it is single stranded and contains adenine it is single stranded and has paired nitrogen bases ...
... it is single stranded and contains uracil it is single stranded and contains thymine it is single stranded and contains adenine it is single stranded and has paired nitrogen bases ...
Federal Agency for Social Development
... long. The DNA is not associated with protein or histones as in eukaryotic cell chromosomes. Since the DNA is about 1000 times longer than the cell, it is obvious that it is not arranged as a simple circle; electron microscopy of thin sections of bacteria shows the nuclear body as an irregular coiled ...
... long. The DNA is not associated with protein or histones as in eukaryotic cell chromosomes. Since the DNA is about 1000 times longer than the cell, it is obvious that it is not arranged as a simple circle; electron microscopy of thin sections of bacteria shows the nuclear body as an irregular coiled ...
HUA1, a Regulator of Stamen and Carpel Identities
... In hua1-1, the major RNA species was 400 nucleotides longer than the wild-type HUA1 RNA (Figure 4), suggesting that the second intron was retained in most hua1-1 RNA. This major hua1-1 transcript was detected at 12% of the abundance of wild-type HUA1 RNA. Other hua1-1 RNA species of smaller sizes a ...
... In hua1-1, the major RNA species was 400 nucleotides longer than the wild-type HUA1 RNA (Figure 4), suggesting that the second intron was retained in most hua1-1 RNA. This major hua1-1 transcript was detected at 12% of the abundance of wild-type HUA1 RNA. Other hua1-1 RNA species of smaller sizes a ...
Biogenesis of trans-acting siRNAs, endogenous
... SGS3 may act on the miR173-cleaved fragments before RDR6, and SGS3 may stabilize the miR173 cleavage fragments. An extensive characterization of SGS3 protein expressed in Escherichia coli indicated that SGS3 can specifically bind to a dsRNA containing a 5′-overhang, but cannot bind to an ssRNA or a ...
... SGS3 may act on the miR173-cleaved fragments before RDR6, and SGS3 may stabilize the miR173 cleavage fragments. An extensive characterization of SGS3 protein expressed in Escherichia coli indicated that SGS3 can specifically bind to a dsRNA containing a 5′-overhang, but cannot bind to an ssRNA or a ...
video slide - Geneva High School
... synthesis of RNA: a closer look • Transcription, the first stage of gene expression, can be examined in more detail • RNA synthesis is catalyzed by RNA polymerase, which pries the DNA strands apart and hooks together the RNA nucleotides • RNA synthesis follows the same base-pairing rules as DNA, exc ...
... synthesis of RNA: a closer look • Transcription, the first stage of gene expression, can be examined in more detail • RNA synthesis is catalyzed by RNA polymerase, which pries the DNA strands apart and hooks together the RNA nucleotides • RNA synthesis follows the same base-pairing rules as DNA, exc ...
Enzymes are macromolecules that help accelerate (catalyze
... forces that require close contact, a substrate must have a matching shape to fit into the site. However, the active site of some enzymes assume a shape that is complementary to that of the substrate only after the substrate is bound. This process of dynamic recognition is called induced fit. Enzymes ...
... forces that require close contact, a substrate must have a matching shape to fit into the site. However, the active site of some enzymes assume a shape that is complementary to that of the substrate only after the substrate is bound. This process of dynamic recognition is called induced fit. Enzymes ...
PCR
... Fidelity of Taq or error rate is: 1 base misincorporation per 104 nucleotides polymerized for a 400 bp fragment amplied 106 fold (=20 cycles) results in in about 33% of the products carrying a mutation (thus should sequence several PCR amplimers to determine consensus) ...
... Fidelity of Taq or error rate is: 1 base misincorporation per 104 nucleotides polymerized for a 400 bp fragment amplied 106 fold (=20 cycles) results in in about 33% of the products carrying a mutation (thus should sequence several PCR amplimers to determine consensus) ...
Effective treatment of extensive and recalcitrant plantar warts
... Despite of wide acceptance, the clearance rate for plantar warts is only 50% after executing every 3 weeks for a 3-month duration.3 Furthermore, patients with plantar warts tend not to follow up owing to the high-relapse rate, persisting discomfort, and cutaneous secondary bacterial infection. Retin ...
... Despite of wide acceptance, the clearance rate for plantar warts is only 50% after executing every 3 weeks for a 3-month duration.3 Furthermore, patients with plantar warts tend not to follow up owing to the high-relapse rate, persisting discomfort, and cutaneous secondary bacterial infection. Retin ...
4.Genetechnology2
... • Synthesising the gene using an automated gene machine – this method can be used if the amino sequence of the protein gene product is known; the DNA sequence of the gene can be determined by working backwards using the genetic code As most gene products are large proteins, this method is useful mai ...
... • Synthesising the gene using an automated gene machine – this method can be used if the amino sequence of the protein gene product is known; the DNA sequence of the gene can be determined by working backwards using the genetic code As most gene products are large proteins, this method is useful mai ...
genetics: typical test questions
... 31. Why is it necessary for the nitrogenous bases to break apart during DNA replication? The nitrogenous bases/nucleic acids (ATCG) must break apart so the cell can use each strand as a template or guide indicating what new bases should be added to make two complete double helixes. See number 33 for ...
... 31. Why is it necessary for the nitrogenous bases to break apart during DNA replication? The nitrogenous bases/nucleic acids (ATCG) must break apart so the cell can use each strand as a template or guide indicating what new bases should be added to make two complete double helixes. See number 33 for ...
DNA Structure - StudyTime NZ
... Mutations are the only way of creating totally new alleles in a species ...
... Mutations are the only way of creating totally new alleles in a species ...
Single-Molecule Studies Using Magnetic Traps
... in the rapid intracellular transport of vesicles, or in the activation of mechanically gated ion channels at the cell surface. In particular, single-molecule studies highlighted the importance of tension and torsion at the molecular scale for modulating protein–DNA and protein–protein interactions. ...
... in the rapid intracellular transport of vesicles, or in the activation of mechanically gated ion channels at the cell surface. In particular, single-molecule studies highlighted the importance of tension and torsion at the molecular scale for modulating protein–DNA and protein–protein interactions. ...
Breast Cancer
... called Short Tandem Repeats (abbreviated STRs and also called microsatellites). An STR is a region of DNA composed of a short sequence of nucleotides repeated many times. The number of repeated sequences in a given STR varies from person to person. The alternate forms of a given STR correspond with ...
... called Short Tandem Repeats (abbreviated STRs and also called microsatellites). An STR is a region of DNA composed of a short sequence of nucleotides repeated many times. The number of repeated sequences in a given STR varies from person to person. The alternate forms of a given STR correspond with ...
Sequence logos for DNA sequence alignments
... that position, while the height of symbols within the stack indicates the relative frequency of each amino or nucleic acid at that position. In general, a sequence logo provides a richer and more precise description of, for example, a binding site, than would a consensus sequence. ...
... that position, while the height of symbols within the stack indicates the relative frequency of each amino or nucleic acid at that position. In general, a sequence logo provides a richer and more precise description of, for example, a binding site, than would a consensus sequence. ...
Ch12_lecture - Dr. Brahmbhatt`s Class Handouts
... 12.3 How Is Biotechnology Used In Forensic Science? Gel electrophoresis separates DNA segments. • A mixture of DNA pieces is separated by a technique called gel electrophoresis. • The mixture of DNA is loaded onto a slab of agarose. • The gel is put in a chamber with electrodes connected to each ...
... 12.3 How Is Biotechnology Used In Forensic Science? Gel electrophoresis separates DNA segments. • A mixture of DNA pieces is separated by a technique called gel electrophoresis. • The mixture of DNA is loaded onto a slab of agarose. • The gel is put in a chamber with electrodes connected to each ...
Ch8 Cell Reproduction
... • The point at which the sister chromatids are connected is called the centromere • The kinetochore is the part of the centromere that attaches to the ...
... • The point at which the sister chromatids are connected is called the centromere • The kinetochore is the part of the centromere that attaches to the ...
BioInformatics Tools ppt
... aligning amino acids called PAM-250. The score of the block is the sum of the scores assigned to each of its columns. Score of the column is the sum of all pairwise similarity scores of the amino acids it comprises. Those SP scores are called “Sum of the Pairs”. MACAW can use some different, more bi ...
... aligning amino acids called PAM-250. The score of the block is the sum of the scores assigned to each of its columns. Score of the column is the sum of all pairwise similarity scores of the amino acids it comprises. Those SP scores are called “Sum of the Pairs”. MACAW can use some different, more bi ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.