Characterization of microbial activity in the surface layers of a
... large amount of information on the kinetics of substrate adsorption/desorption. Since only a small portion of the available substrate is bound and since the binding is reversible, it does not appear that sediment substrate binding has any effect on these types of studies. Additional experiments prov ...
... large amount of information on the kinetics of substrate adsorption/desorption. Since only a small portion of the available substrate is bound and since the binding is reversible, it does not appear that sediment substrate binding has any effect on these types of studies. Additional experiments prov ...
Adaptive evolution without natural selection
... by individual use or exercise, as in structures which are so improved’ (Osborn, 1897: 584–585). To understand this hesitation (which has been continuously used in the interpretations of the Baldwin effect), I should point out that there was almost no knowledge about the dynamics of gene expression p ...
... by individual use or exercise, as in structures which are so improved’ (Osborn, 1897: 584–585). To understand this hesitation (which has been continuously used in the interpretations of the Baldwin effect), I should point out that there was almost no knowledge about the dynamics of gene expression p ...
types of gel - WordPress.com
... TYPES OF GEL 1. Agarose Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experime ...
... TYPES OF GEL 1. Agarose Agarose gels are made from the natural polysaccharide polymers extracted from seaweed. Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experime ...
Bio II Ch 19 Eukaryotic Genomes
... eukaryotic chromatin is far more complex. • Eukaryotic DNA is precisely combined with large amounts of protein. • During interphase of the cell cycle, chromatin fibers are usually highly extended within the nucleus. • During mitosis, the chromatin coils and condenses to ...
... eukaryotic chromatin is far more complex. • Eukaryotic DNA is precisely combined with large amounts of protein. • During interphase of the cell cycle, chromatin fibers are usually highly extended within the nucleus. • During mitosis, the chromatin coils and condenses to ...
Detection of genetically modified cotton seeds using PCR
... (30 sec each) beginning at 50°C for melting curve analysis to confirm the specificity of the amplification products. Thermocycling was performed in a final volume of 25 µL (10.5 µL of water, 0.2 µM of each primer, 1 µL of genomic DNA and 12.5 µL of 2X iQ SYBR Green Supermix; Bio-Rad). The real-time ...
... (30 sec each) beginning at 50°C for melting curve analysis to confirm the specificity of the amplification products. Thermocycling was performed in a final volume of 25 µL (10.5 µL of water, 0.2 µM of each primer, 1 µL of genomic DNA and 12.5 µL of 2X iQ SYBR Green Supermix; Bio-Rad). The real-time ...
M2 RNA Pol Ⅰ genes
... Upstream regulatory elements • The basal elements (the TATA box and initiator elements) : primarily determine the location of the startpoint, and sponsor initiation only at a rather low level. • Upstream regulatory elements (URE) such as the SP1 box and CCAAT boxes, greatly increase the frequency of ...
... Upstream regulatory elements • The basal elements (the TATA box and initiator elements) : primarily determine the location of the startpoint, and sponsor initiation only at a rather low level. • Upstream regulatory elements (URE) such as the SP1 box and CCAAT boxes, greatly increase the frequency of ...
Chlamydia exploit the mammalian tryptophan-depletion
... The highly expansive distribution of TyrP orthologs and paralogs in Chlamydiales Chlamydiaceae Distribution of tyrP genes in Chlamydiales Lysosomal degradation as a source of Trp Chlamydiaceae are the sole taxon family within Chlamydiales to deploy the recently recognized DH6N pathway of menaquinon ...
... The highly expansive distribution of TyrP orthologs and paralogs in Chlamydiales Chlamydiaceae Distribution of tyrP genes in Chlamydiales Lysosomal degradation as a source of Trp Chlamydiaceae are the sole taxon family within Chlamydiales to deploy the recently recognized DH6N pathway of menaquinon ...
Exercise 1: RNA
... In addition to using high throughput sequencing technology for DNA sequencing, with for example the goal of discovering nucleotide variants, it can also be applied to RNA sequencing – in this case, it can be used for discovery and for quantification. RNA sequencing has the potential to also provide ...
... In addition to using high throughput sequencing technology for DNA sequencing, with for example the goal of discovering nucleotide variants, it can also be applied to RNA sequencing – in this case, it can be used for discovery and for quantification. RNA sequencing has the potential to also provide ...
Development of an internally controlled real-time PCR
... positive pigeon samples were not tested against a recognised standard method, these samples were tested as highly suspected animal reservoirs. The detection of C. psittaci DNA in three of nine pigeons with a possible clinical picture of C. psittaci infection is highly suggestive of true disease. In ...
... positive pigeon samples were not tested against a recognised standard method, these samples were tested as highly suspected animal reservoirs. The detection of C. psittaci DNA in three of nine pigeons with a possible clinical picture of C. psittaci infection is highly suggestive of true disease. In ...
Scientists Say Human Genome Is Complete
... Scientists praised the Human Genome Project for its further three years of hard work and for producing a resource of enormous value to research. But several qualified their admiration by noting that even if the project is complete, the human genome is not. The parts of the genome still missing are o ...
... Scientists praised the Human Genome Project for its further three years of hard work and for producing a resource of enormous value to research. But several qualified their admiration by noting that even if the project is complete, the human genome is not. The parts of the genome still missing are o ...
Gene Section FANCC (Fanconi anaemia complementation group C) Atlas of Genetics and Cytogenetics
... 16 years: patients die of bone marrow failure (infections, haemorrhages), leukaemia, or androgen therapy related liver tumours. It has recently been shown that significant phenotypic differences were found between the various complementation groups. FA group C patients had less somatic abnormalities ...
... 16 years: patients die of bone marrow failure (infections, haemorrhages), leukaemia, or androgen therapy related liver tumours. It has recently been shown that significant phenotypic differences were found between the various complementation groups. FA group C patients had less somatic abnormalities ...
Gene sequences useful for predicting relatedness of whole
... identified (Table 1). It was known that the genera Escherichia, Salmonella and Shigella were highly related (Brenner et al., 1969, 1972; Crosa et al., 1973), so they were treated as if they belonged to a single genus. Genome sequences within each of the 16 groups were aligned in pairwise fashion by ...
... identified (Table 1). It was known that the genera Escherichia, Salmonella and Shigella were highly related (Brenner et al., 1969, 1972; Crosa et al., 1973), so they were treated as if they belonged to a single genus. Genome sequences within each of the 16 groups were aligned in pairwise fashion by ...
Isr J Chem (2010) - Weizmann Institute of Science
... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
P. Mignon, J. Steyaert, R. Loris, P. Geerlings, and S. Loverix, J. Biol
... Tyr-38, His-40, Glu-58, Arg-77, His-92, and Phe-100 (see Fig. 2a). With the exception of Arg-77, all these amino acids have been shown to take part in catalysis (5). Both the His-40 and Glu-58 side chains are in the direct vicinity of the 2⬘-nucleophile. pH dependence studies have shown Glu-58 to be ...
... Tyr-38, His-40, Glu-58, Arg-77, His-92, and Phe-100 (see Fig. 2a). With the exception of Arg-77, all these amino acids have been shown to take part in catalysis (5). Both the His-40 and Glu-58 side chains are in the direct vicinity of the 2⬘-nucleophile. pH dependence studies have shown Glu-58 to be ...
3 LECTURES ON "DELEUZE AND BIOLOGY" John Protevi LSU
... counts is differential reproduction, no matter how it’s achieved. My colleague Dominique Homberger writes: this scenario needs considerable nuancing, as it presupposes a fixed environment against the “borders” of which immobile species press (a container image). But organisms are mobile and environm ...
... counts is differential reproduction, no matter how it’s achieved. My colleague Dominique Homberger writes: this scenario needs considerable nuancing, as it presupposes a fixed environment against the “borders” of which immobile species press (a container image). But organisms are mobile and environm ...
記錄 編號 3862 狀態 NC090FJU00112010 助教 查核 索書 號 學校
... insertion sequence IS100. FEMS Microbiol. Lett. 121: 269-274. Polard, P., and Chandler, M. 1995. Bacterial transposases and retroviral integrases. Mol. Microbiol. 15: 13-23. Poussier, S., Vandewalle, P., and Luisetti, J. 1999. Genetic diversity of African and worldwide strains of Ralstonia solanacea ...
... insertion sequence IS100. FEMS Microbiol. Lett. 121: 269-274. Polard, P., and Chandler, M. 1995. Bacterial transposases and retroviral integrases. Mol. Microbiol. 15: 13-23. Poussier, S., Vandewalle, P., and Luisetti, J. 1999. Genetic diversity of African and worldwide strains of Ralstonia solanacea ...
Using an Alu Insertion Polymorphism to Study Human
... Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety prec ...
... Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety prec ...
Nucleosomal structure of sea urchin and starfish sperm chromatin
... histone H2B from sea urchin sperm could be reflected in chromatin structure we compared using micrococcal nuclease some parameters of sea urchin and starfish sperm chromatin. Starfish sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, see ...
... histone H2B from sea urchin sperm could be reflected in chromatin structure we compared using micrococcal nuclease some parameters of sea urchin and starfish sperm chromatin. Starfish sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, see ...
A new ferrochelatase mutation combined with low
... family. Our expression studies in E. coli cells clearly showed that the mutant ferrochelatase with the O4 deletion was translated as no immunologically detectable protein or enzyme activity (Figure 6). The O4 deletion, which results in six abnormal amino acid residues after amino acid 192, caused a ...
... family. Our expression studies in E. coli cells clearly showed that the mutant ferrochelatase with the O4 deletion was translated as no immunologically detectable protein or enzyme activity (Figure 6). The O4 deletion, which results in six abnormal amino acid residues after amino acid 192, caused a ...
2- pcr primer design and reaction optimisation
... the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a 1⁄4 chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (eg. AG); a 1/256 chance of finding a given 4-base seque ...
... the likelihood of annealing to sequences other than the chosen target is very low. For example, there is a 1⁄4 chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (eg. AG); a 1/256 chance of finding a given 4-base seque ...
BC 367 Experiment 4 Kinetic Properties of Acid Phosphatase
... study of the kinetics of enzyme-catalyzed reactions. They found that the dependence of the rate of enzymatic reactions on the substrate concentration was generally hyperbolic: ...
... study of the kinetics of enzyme-catalyzed reactions. They found that the dependence of the rate of enzymatic reactions on the substrate concentration was generally hyperbolic: ...
Deoxyribozyme
_DNAzyme.png?width=300)
Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.