Phylogenetic, amino acid content and indel analyses
... sequences encoding conserved amino acid sequences in the rpoB genes of Mycobacterium tuberculosis (EMBL accession no. U12205), Mycobacterium leprae (Z14314), Mycobacterium smegmatis (U24494), Bacillus subtilis (L24376) and Staphylococcus aureus (X64172) and the rplL genes of Escherichia coli (V00339 ...
... sequences encoding conserved amino acid sequences in the rpoB genes of Mycobacterium tuberculosis (EMBL accession no. U12205), Mycobacterium leprae (Z14314), Mycobacterium smegmatis (U24494), Bacillus subtilis (L24376) and Staphylococcus aureus (X64172) and the rplL genes of Escherichia coli (V00339 ...
How Genes and Genomes Evolve
... • Adult mammalian females are genetic mosaics (with different alleles functioning in different cells) – A. This is true since paternal & maternal X chromosomes may have different alleles for same trait – B. X-linked pigment genes in cats – calico – C. Pigmentation genes in humans are not found on X ...
... • Adult mammalian females are genetic mosaics (with different alleles functioning in different cells) – A. This is true since paternal & maternal X chromosomes may have different alleles for same trait – B. X-linked pigment genes in cats – calico – C. Pigmentation genes in humans are not found on X ...
The Living World
... 10.1 A scientific Revolution Genetic engineering is the process of moving genes from one organism to another Having a major impact on agriculture & medicine Fig. 10.1 ...
... 10.1 A scientific Revolution Genetic engineering is the process of moving genes from one organism to another Having a major impact on agriculture & medicine Fig. 10.1 ...
MOLECULAR BIOLOGY AND BIOTECHNOLOGY
... MBB 502/ FUNDAMENTALS OF MOLECULAR BIOLOGY 3+0 SEM - I BIOCHEM 504/ (To be taught jointly by Molecular Biology & Biotechnology, Biochemistry and BIF508 Bioinformatics) ObjectiveT o familiarize the students with the basic cellular processes at molecular level. Theory UNIT-I: Historical developments o ...
... MBB 502/ FUNDAMENTALS OF MOLECULAR BIOLOGY 3+0 SEM - I BIOCHEM 504/ (To be taught jointly by Molecular Biology & Biotechnology, Biochemistry and BIF508 Bioinformatics) ObjectiveT o familiarize the students with the basic cellular processes at molecular level. Theory UNIT-I: Historical developments o ...
Genotyping of Ryanodine receptor 1 (RYR1) gene associated with
... resolution melting (HRM) method has been used as a high-throughput molecular genotyping approach for detection of variation on MAS marker associated with economic traits in pig for many years. Although the HRM is a capable method to identify mutation in the RYR1 gene, it is demonstrated that subopti ...
... resolution melting (HRM) method has been used as a high-throughput molecular genotyping approach for detection of variation on MAS marker associated with economic traits in pig for many years. Although the HRM is a capable method to identify mutation in the RYR1 gene, it is demonstrated that subopti ...
Rapid detection of multidrug-resistant tuberculosis
... eye, and stored using any simple word-processing programme. Strain-typing itself does not provide direct information about drug susceptibility, but a register of spoligotypes from known MDR isolates may be particularly useful in providing early warning of possible infection with these strains. Whils ...
... eye, and stored using any simple word-processing programme. Strain-typing itself does not provide direct information about drug susceptibility, but a register of spoligotypes from known MDR isolates may be particularly useful in providing early warning of possible infection with these strains. Whils ...
Chapter 1: Biochemistry in the Modern World
... organisms rather than cells. In ecology, for example, ecosystems are often described in terms of food webs, with energy being generated by photosynthesis and then transferred up a food chain through the herbivores to the top carnivores (Fig. 1.2). The generation of energy is a central topic in bioch ...
... organisms rather than cells. In ecology, for example, ecosystems are often described in terms of food webs, with energy being generated by photosynthesis and then transferred up a food chain through the herbivores to the top carnivores (Fig. 1.2). The generation of energy is a central topic in bioch ...
SNP Analysis of the PTC Gene Using PCR
... Individuals vary greatly in their sensitivity to the bitter compound PTC (Figure 2). This fact was discovered in 1931 in a series of events that involved impressive scientific curiosity and questionable laboratory safety. A chemist named Arthur Fox was mixing a powdered chemical when he accidentally ...
... Individuals vary greatly in their sensitivity to the bitter compound PTC (Figure 2). This fact was discovered in 1931 in a series of events that involved impressive scientific curiosity and questionable laboratory safety. A chemist named Arthur Fox was mixing a powdered chemical when he accidentally ...
File
... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
number of fifty human tumours
... of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approximately twice that expected from the DNA data. This discrepancy is possibly d ...
... of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approximately twice that expected from the DNA data. This discrepancy is possibly d ...
TAR-RNA binding by HIV-1 Tat protein is
... Nolte’s 38mer L-RNA, originally developed as a ligand for L-arginine, is sequence-wise unrelated to TAR and binds to a short peptide (12 residues) corresponding to the basic region of HIV-1 Tat protein with a Kd of 26 µM. In contrast, our competition binding experiments demonstrate that the affinity ...
... Nolte’s 38mer L-RNA, originally developed as a ligand for L-arginine, is sequence-wise unrelated to TAR and binds to a short peptide (12 residues) corresponding to the basic region of HIV-1 Tat protein with a Kd of 26 µM. In contrast, our competition binding experiments demonstrate that the affinity ...
Chapter 26 - s3.amazonaws.com
... If ATP c.c. for a reaction in one direction differs from c.c. in the other, the reactions can form a substrate cycle • The point is not that ATP can be consumed by cycling • But rather that the difference in c.c. permits both reactions (pathways) to be thermodynamically favorable at all times • Allo ...
... If ATP c.c. for a reaction in one direction differs from c.c. in the other, the reactions can form a substrate cycle • The point is not that ATP can be consumed by cycling • But rather that the difference in c.c. permits both reactions (pathways) to be thermodynamically favorable at all times • Allo ...
3D Structures of Biological Macromolecules Jürgen Sühnel
... Human intestinal trefoil factor [NMR structure with 85 models] Human intestinal trefoil factor, hITF, a secretory polypeptide found mainly in the human gastrointestinal tract, is a member of the newly characterized trefoil factor or P-domain peptide family representing putative growth factors. ...
... Human intestinal trefoil factor [NMR structure with 85 models] Human intestinal trefoil factor, hITF, a secretory polypeptide found mainly in the human gastrointestinal tract, is a member of the newly characterized trefoil factor or P-domain peptide family representing putative growth factors. ...
Gapped Blast and PSI
... The DNA sequences used in comparison are usually conceptually transcribed before comparison. BLAST programs use a threshold value which can be adjusted to alter speed and probability. A higher value of T will give greater speed, but also a larger probability of missing weaker similarities. Can use v ...
... The DNA sequences used in comparison are usually conceptually transcribed before comparison. BLAST programs use a threshold value which can be adjusted to alter speed and probability. A higher value of T will give greater speed, but also a larger probability of missing weaker similarities. Can use v ...
PPT - FLI - Leibniz Institute for Age Research
... Human intestinal trefoil factor [NMR structure with 85 models] Human intestinal trefoil factor, hITF, a secretory polypeptide found mainly in the human gastrointestinal tract, is a member of the newly characterized trefoil factor or P-domain peptide family representing putative growth factors. ...
... Human intestinal trefoil factor [NMR structure with 85 models] Human intestinal trefoil factor, hITF, a secretory polypeptide found mainly in the human gastrointestinal tract, is a member of the newly characterized trefoil factor or P-domain peptide family representing putative growth factors. ...
AMPKA1B2G1 Master Q102-1
... protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998. ...
... protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998. ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... encoding a protein of 491 amino acids [24]. In addition to the wildtype allele, several CYP2B6 allelic variants (C64T, C777A, A785G, C1459T, G516T, A785G, G516T, A785G, C1459T, A415G, and G516T) have been identified [23,26]. Previous reports have found that CYP2B6*6 (G516T and A785G) was associated ...
... encoding a protein of 491 amino acids [24]. In addition to the wildtype allele, several CYP2B6 allelic variants (C64T, C777A, A785G, C1459T, G516T, A785G, G516T, A785G, C1459T, A415G, and G516T) have been identified [23,26]. Previous reports have found that CYP2B6*6 (G516T and A785G) was associated ...
before
... a mutation in the gene that codes for hemoglobin causes a single amino acid substitution in the protein, RBC shape changes from round to sickle shape ...
... a mutation in the gene that codes for hemoglobin causes a single amino acid substitution in the protein, RBC shape changes from round to sickle shape ...
Viruses & Microorganisms
... 2. They contain RNA, but their host cell’s genetic material is DNA. ...
... 2. They contain RNA, but their host cell’s genetic material is DNA. ...
lab07.doc
... window normally only shows Python files (with a .py or similar extension), so you will probably have to tell it to show all files (*.* or *) before you can open them. Do not open these files in a word processing application (eg: Microsoft Word). You will notice that these sequences are long enough t ...
... window normally only shows Python files (with a .py or similar extension), so you will probably have to tell it to show all files (*.* or *) before you can open them. Do not open these files in a word processing application (eg: Microsoft Word). You will notice that these sequences are long enough t ...
Clostridium hiranonis sp. nov., a human intestinal bacterium with
... to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 were 31n1 and 31n9 mol %, respectively. The levels of DNA–DNA hybridization between strains TO-931T and HD-17 ...
... to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 were 31n1 and 31n9 mol %, respectively. The levels of DNA–DNA hybridization between strains TO-931T and HD-17 ...
Comparación de secuencias
... particular size. It decreases exponentially with the Score (S) that is assigned to a match between two sequences. Essentially, the E value describes the random background noise that exists for matches between sequences. For example, an E value of 1 assigned to a hit can be interpreted as meaning tha ...
... particular size. It decreases exponentially with the Score (S) that is assigned to a match between two sequences. Essentially, the E value describes the random background noise that exists for matches between sequences. For example, an E value of 1 assigned to a hit can be interpreted as meaning tha ...
Deoxyribozyme
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Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.