Biochemical, biophysical and interaction studies of the stress
... advice on PhD related issues and CD experiments. Also I would like to thank Dr Marija Kristic Demonacos for her help when carrying out the molecular cloning aspect of this project and letting me work on her bench space, also providing me with an hSTRAP construct (pHA1-hSTRAP(1-440)) which was used i ...
... advice on PhD related issues and CD experiments. Also I would like to thank Dr Marija Kristic Demonacos for her help when carrying out the molecular cloning aspect of this project and letting me work on her bench space, also providing me with an hSTRAP construct (pHA1-hSTRAP(1-440)) which was used i ...
36. Amino Acids and Carbohydrates in Sediments and Interstitial
... carbohydrates in sediments are dominated by glucose, galactose, and mannose, while dissolved sugars are dominated by glucose and fructose. In contrast to the lack of correlation between abundances of bulk TOC and DOC in corresponding interstitial waters, amino compounds and sugars do show some corre ...
... carbohydrates in sediments are dominated by glucose, galactose, and mannose, while dissolved sugars are dominated by glucose and fructose. In contrast to the lack of correlation between abundances of bulk TOC and DOC in corresponding interstitial waters, amino compounds and sugars do show some corre ...
Slide 1
... 3.12 Proteins are made from amino acids linked by peptide bonds • Protein diversity – Is based on different arrangements of a common set of 20 amino acid monomers ...
... 3.12 Proteins are made from amino acids linked by peptide bonds • Protein diversity – Is based on different arrangements of a common set of 20 amino acid monomers ...
PDF - Yeh Lab
... although their time courses still exhibited improved linearity. The time course for S3, in which the 3 amino acids Gln6-Val8 of the tyrocidine sequence were replaced with the spacer 8-amino-3,6-dioxaoctanoic acid, is shown in Figure 2B. The factors that govern these differences are unknown. However, ...
... although their time courses still exhibited improved linearity. The time course for S3, in which the 3 amino acids Gln6-Val8 of the tyrocidine sequence were replaced with the spacer 8-amino-3,6-dioxaoctanoic acid, is shown in Figure 2B. The factors that govern these differences are unknown. However, ...
Plasmodium falciparum enolase - Tata Institute of Fundamental
... E. coli cells were transformed with the recombinant plasmid as described above (Materials and methods). The cloned gene was subjected to DNA sequencing, and the full amino-acid sequence of the recombinant protein was deduced. The amino-acid sequence was found to be identical with the 3D7 strain. How ...
... E. coli cells were transformed with the recombinant plasmid as described above (Materials and methods). The cloned gene was subjected to DNA sequencing, and the full amino-acid sequence of the recombinant protein was deduced. The amino-acid sequence was found to be identical with the 3D7 strain. How ...
Physiology of Saccharomyces cerevisiae in anaerobic glucose
... (Postma et al., 1989~).This procedure yielded the same results as drying at 80 "C on filters or in weighing-flasks. Parallel samples varied by less than 2%. Analysis of metabolites. Acetate, acetaldehyde, glycerol, succinate and glucose were determined with Boehringer test-kits 148261,668613, 148270 ...
... (Postma et al., 1989~).This procedure yielded the same results as drying at 80 "C on filters or in weighing-flasks. Parallel samples varied by less than 2%. Analysis of metabolites. Acetate, acetaldehyde, glycerol, succinate and glucose were determined with Boehringer test-kits 148261,668613, 148270 ...
BioFiles v5 n1 - Sigma
... After biosynthesis, Neu5Ac must be incorporated as a nucleotide donor substrate for subsequent transfer to an oligosaccharide structure. This substrate creation takes place in the nucleus of eukaryotic cells. Neu5Ac is transported to the nucleus; whereupon, it is attached to cytidine 5′-triphosphate ...
... After biosynthesis, Neu5Ac must be incorporated as a nucleotide donor substrate for subsequent transfer to an oligosaccharide structure. This substrate creation takes place in the nucleus of eukaryotic cells. Neu5Ac is transported to the nucleus; whereupon, it is attached to cytidine 5′-triphosphate ...
Amino Acid Sequencing
... proteins. By comparing the amino acid sequences in homologous proteins of similar organisms and of diverse organisms, evolutionary relationships that might otherwise go undetected can be determined. Biologists believe that the greater the similarity between the amino acid sequences of two organisms, ...
... proteins. By comparing the amino acid sequences in homologous proteins of similar organisms and of diverse organisms, evolutionary relationships that might otherwise go undetected can be determined. Biologists believe that the greater the similarity between the amino acid sequences of two organisms, ...
Infant Formula And Medical Foods
... differently. Iron Content per liter when prepared as directed. Milligrams (mg) per liter (L) unless specified differently. Yield in fl oz of one oz can of powder, when mixed to standard dilution. Nutrient Source Listed in order by weight Protein Source (% kcal from protein) Carbohydrate Source (% kc ...
... differently. Iron Content per liter when prepared as directed. Milligrams (mg) per liter (L) unless specified differently. Yield in fl oz of one oz can of powder, when mixed to standard dilution. Nutrient Source Listed in order by weight Protein Source (% kcal from protein) Carbohydrate Source (% kc ...
213 lactate dehydrog..
... cytoplasm (glycolysis) and mitochondria (Krebs' cycle) In the presence of O2 pyruvate (the product of glycolysis) passes by special pyruvate transporter into mitochondria which proceeds as follows: 1. Oxidative decarboxylation of pyruvate into acetyl CoA. 2. Acetyl CoA is then oxidized completely to ...
... cytoplasm (glycolysis) and mitochondria (Krebs' cycle) In the presence of O2 pyruvate (the product of glycolysis) passes by special pyruvate transporter into mitochondria which proceeds as follows: 1. Oxidative decarboxylation of pyruvate into acetyl CoA. 2. Acetyl CoA is then oxidized completely to ...
Electrophoresis Basi..
... Proteins and Electrophoresis Isozymes (isoenzymes): enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes have different molecular weights and chemical properties and thus can be separated from each other during electrophoresis. Isozymes are coded by genes on d ...
... Proteins and Electrophoresis Isozymes (isoenzymes): enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes have different molecular weights and chemical properties and thus can be separated from each other during electrophoresis. Isozymes are coded by genes on d ...
univERsity oF copEnhAGEn
... of 15-20 min an arterial blood sample was taken for control of blood gases and pH. The ‘flushing gas-mixture’ was then changed to 20% C 0 2 and 20% O2 in nitrogen, and after about 10s of hypercapnia changed to 10%C 0 2 and 20% 0, in nitrogen. Brain tissue was sampled for analysis by means of ‘freeze ...
... of 15-20 min an arterial blood sample was taken for control of blood gases and pH. The ‘flushing gas-mixture’ was then changed to 20% C 0 2 and 20% O2 in nitrogen, and after about 10s of hypercapnia changed to 10%C 0 2 and 20% 0, in nitrogen. Brain tissue was sampled for analysis by means of ‘freeze ...
H INHIBITORS OF HIV-1 REVERSE TRANSCRIPTASE Research Article
... conformations of the ligands in pdb format[17]. Energy minimized conformation of ligands were subjected to calculation of GasteigerHuckel charges and saved in default format of Autodock. Autodock generated 50 possible binding conformations i.e., 50 runs for each docking by using LGA search. Default ...
... conformations of the ligands in pdb format[17]. Energy minimized conformation of ligands were subjected to calculation of GasteigerHuckel charges and saved in default format of Autodock. Autodock generated 50 possible binding conformations i.e., 50 runs for each docking by using LGA search. Default ...
Journal of Bacteriology
... //taxonomy.zoology.gla.ac.uk/rod/treeview.html). Programs used to reconstruct the phylogenetic tree were obtained from the PHYLIP (v.3.69) software package (http://evolution.genetics.washington.edu/phylip). ...
... //taxonomy.zoology.gla.ac.uk/rod/treeview.html). Programs used to reconstruct the phylogenetic tree were obtained from the PHYLIP (v.3.69) software package (http://evolution.genetics.washington.edu/phylip). ...
1 Biosynthesis and Chemical Properties of Natural Substances in
... vulgare). A compound from the Asteraceae Carlina acaulis has a strong antimicrobial activity and is also toxic for trypanosomes. Based on their chemical structures, the secondary metabolites can be placed into different classes (Table 1.1). The synthesis of secondary metabolites can occur in all pla ...
... vulgare). A compound from the Asteraceae Carlina acaulis has a strong antimicrobial activity and is also toxic for trypanosomes. Based on their chemical structures, the secondary metabolites can be placed into different classes (Table 1.1). The synthesis of secondary metabolites can occur in all pla ...
Regulators of Ubiquitin Dependent Protein Degradation in the
... ubiquitin ligases (E3s). One group of E3s contains cullins as the core component. Activity of these cullin containing E3s is modulated by the covalent modification of cullin with the ubiquitin like protein Nedd8. In this work three regulators of cullin containing E3s were studied in the fungus Asper ...
... ubiquitin ligases (E3s). One group of E3s contains cullins as the core component. Activity of these cullin containing E3s is modulated by the covalent modification of cullin with the ubiquitin like protein Nedd8. In this work three regulators of cullin containing E3s were studied in the fungus Asper ...
αII-Spectrin interacts with Tes and EVL, two actin
... interactions, defining the multiple physiological functions of spectrins. Functions of the spectrin-based skeleton are well-defined in red blood cells where it is required for shape maintenance, for resistance to shear stress and for deformability. However, the functions of the more complex spectrin ...
... interactions, defining the multiple physiological functions of spectrins. Functions of the spectrin-based skeleton are well-defined in red blood cells where it is required for shape maintenance, for resistance to shear stress and for deformability. However, the functions of the more complex spectrin ...
Journal of Molecular Microbiology and Biotechnology
... and C2, located in BmyC. The modules B1, B2, and C1 also contain epimerization domains, directing conversion of amino acids 2, 3, and 6 in a D-configuration. The last domain of this multienzyme system is a thioesterase domain, which is presumably required for release and circularization of the synth ...
... and C2, located in BmyC. The modules B1, B2, and C1 also contain epimerization domains, directing conversion of amino acids 2, 3, and 6 in a D-configuration. The last domain of this multienzyme system is a thioesterase domain, which is presumably required for release and circularization of the synth ...
Structure of human cystathionine synthase: a
... the orientation of their ring planes normal to the protein surface (Figure 2C). The heme is bound in a hydrophobic pocket formed by residues 50±67, a-helices 6 and 8 and a loop preceding b-strand 10 (Figure 3B). The sulfhydryl group of Cys52 and the Ne2 atom of His65 axially coordinate the iron in t ...
... the orientation of their ring planes normal to the protein surface (Figure 2C). The heme is bound in a hydrophobic pocket formed by residues 50±67, a-helices 6 and 8 and a loop preceding b-strand 10 (Figure 3B). The sulfhydryl group of Cys52 and the Ne2 atom of His65 axially coordinate the iron in t ...
PDF - FEMS Microbiology Letters
... cloning event produces a truncated protein of 541 aminoacid residues, lacking 84 amino-acid residues of the Cterminus. In order to solve this cloning problem, primer 98 was designed. Following the same strategy described above, plasmid pAM3 was obtained by cloning the 1.9 kb DNA fragment containing ...
... cloning event produces a truncated protein of 541 aminoacid residues, lacking 84 amino-acid residues of the Cterminus. In order to solve this cloning problem, primer 98 was designed. Following the same strategy described above, plasmid pAM3 was obtained by cloning the 1.9 kb DNA fragment containing ...
Nitrogen Acquisition and Amino Acid Metabolism
... c. This slide just shows the organization of the sequences of each enzyme. XVI. Organisms Gain Access to Atmospheric N2 Via the Pathway of Nitrogen Fixation [S16] a. Nitrogen fixing bacteria can be free living or symbiotic with eukaryotic organisms. b. Bacteria like Rhizobia hook to leguminous plant ...
... c. This slide just shows the organization of the sequences of each enzyme. XVI. Organisms Gain Access to Atmospheric N2 Via the Pathway of Nitrogen Fixation [S16] a. Nitrogen fixing bacteria can be free living or symbiotic with eukaryotic organisms. b. Bacteria like Rhizobia hook to leguminous plant ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.