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Biochemistry and the Cell - Tanque Verde Unified District
Biochemistry and the Cell - Tanque Verde Unified District

... the 3D shape of the protein (polypeptide) chain (4) Quaternary structure a collection of separate polypeptide chains linked together. ...
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... passes out candy. Explain that the Mitochondrion produces most of the ATP in cells. ATP is the primary energy currency of the cell: it is needed for transport work (e.g. exocytosis), mechanical work (e.g. muscle contraction), and chemical work (enzymatic reactions and synthesis of macromolecules). A ...
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... Editorial: RNA and Protein Mutations Lead to New Functional Chemosensory Protein It is just the way imposed by the genetic code in the deep inner core of tissue cells in all organisms. A series of defined triplets in the DNA encodes for a precise combination of amino acids at the protein level. ATGG ...
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... stem-loop structure in the RNA, which we found increases protein expression. The over-lapping sequence in these two PCR products is the TEV protease cleavage site. The resulting transcribed overlap extension PCR product achieves protein translation at ~20-30% of the level obtained from genes first c ...
Reversed-Phase High-Performance Liquid Chromatography
Reversed-Phase High-Performance Liquid Chromatography

Reversed-Phase High-Performance Liquid
Reversed-Phase High-Performance Liquid

... (molecular mass > 10 KDa) and globular proteins. The combination of the traditionally used acidic buffering systems and the hydrophobicity of the n-alkylsilica supports which can result in low mass yields or the loss of biological activity of larger polypeptides and proteins have often discouraged p ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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