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The Skinny on Low-Carbohydrate Diets
The Skinny on Low-Carbohydrate Diets

... Thanks to Michael Eades, M.D. for graphing the results from these two studi ...
Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes
Characterization of the Cobalamin (Vitamin B12) Biosynthetic Genes

... encoded in the genomes of Salmonella spp. and other enteric bacteria (23, 93, 98). (ii) Ethanolamine ammonia lyase degrades ethanolamine to acetaldehyde and ammonia. Salmonella spp. can use ethanolamine as a carbon and/or nitrogen source under aerobic conditions when exogenous cobalamin or cobinamid ...
and Mountain Lions (Puma concolor) in North America
and Mountain Lions (Puma concolor) in North America

... these two geographic regions are also infected with PLVA, and to date, this is the only strain of lentivirus identified in bobcats. We sequenced full-length PLV genomes in order to characterize the molecular evolution of PLV in bobcats and mountain lions. Low sequence homology (88% average pairwise ...
Cytosolic Hsp70 and co-chaperones constitute a novel system for
Cytosolic Hsp70 and co-chaperones constitute a novel system for

... Cell biology ...
Mitochondrial trans-2-Enoyl-CoA Reductase of Wax Ester
Mitochondrial trans-2-Enoyl-CoA Reductase of Wax Ester

... anaerobic conditions. Using NADH, the recombinant enzyme accepted crotonyl-CoA (km ⴝ 68 ␮M) and trans-2hexenoyl-CoA (km ⴝ 91 ␮M). In the crotonyl-CoA-dependent reaction, both NADH (km ⴝ 109 ␮M) or NADPH (km ⴝ 119 ␮M) were accepted, with 2–3-fold higher specific activities for NADH relative to NADPH. ...
A fatty acid
A fatty acid

... Fats must first be broken down to form ...
The Biochemical Machinery of Plastid Envelope
The Biochemical Machinery of Plastid Envelope

... structural and functional diversity and unique biochemical pathways. As such, they are able to transcribe and translate the information present in their own genome but are strongly dependent on imported proteins that are encoded in the nuclear genome and translated in the cytoplasm. Plastids are pre ...
Nonribosomal peptide synthesis in Aspergillus
Nonribosomal peptide synthesis in Aspergillus

... Table 1. NRP synthetase genes of A. fumigatus, with hypothetical NRP synthetase domain architecture based on in silico analysis Key: A, adenylation domain; T, thiolation domain; C, condensation domain; E, epimerase domain; TE, thioesterase domain; DH, COG3320, putative dehydrogenase domain of multif ...
Interaction of the endothelial nitric oxide synthase with the CAT
Interaction of the endothelial nitric oxide synthase with the CAT

... with purified eNOS in order to determine whether the eNOS– CAT-1 interaction is direct or indirect and to determine whether or not a single CAT-1 intracellular domain is sufficient to mediate the interaction. eNOS–CAT-1 association has been proposed as an explanation for the ‘arginine paradox’ [5]. ...
Structure-based design and functional studies of novel noroviral 3C
Structure-based design and functional studies of novel noroviral 3C

Fatty Acid Catabolism Caloric Value of Fats and Carbohydrates
Fatty Acid Catabolism Caloric Value of Fats and Carbohydrates

... FA metabolism is under hormonal regulation. When fuel levels are low, Epinephrine and Glucagon stimulate mobilization of fat and glycogen reserves. Insulin, which is secreted during the fed-state, is anti-lipolytic (it inhibits βoxidation). The transport of FA into mitochondria is allosterically reg ...
Biochem09 - Amit Kessel Ph.D
Biochem09 - Amit Kessel Ph.D

ref. #27 of the TIBS article
ref. #27 of the TIBS article

... Asn-X-Thr/Ser acceptor site (Mellquist et al., 1998; Shakineshleman et al., 1996; our unpublished observations), all such Pro residues in Lep were changed to Gln in the engineered acceptor sites. MGD is a function of the length of the transmembrane segment We have shown that MGDC values vary with th ...
Beta-Adrenergic Receptors and Fat Loss
Beta-Adrenergic Receptors and Fat Loss

... volves either homologous desensitization, where the receptor’s active site is translocated within the cell membrane so that the binding site is no longer positioned extracellularly, or it involves heterologous desensitization, where the receptor is phosphorylated, rendering it incapable of participa ...
Oxidative Phosphorylation - Creighton Chemistry Webserver
Oxidative Phosphorylation - Creighton Chemistry Webserver

... Oxidative Phosphorylation What is mitochondria? 2 membranes: Inner - only permeable to O2, H2O transporters req’d for ATP, Pi, pyruvate, etc. folding increases surface area (site of ox. phos. machinery) Matrix contains: citric acid cycle enzymes Fatty acid oxidation enzymes (discuss later) ...
A siderophore biosynthesis gene cluster from the fish
A siderophore biosynthesis gene cluster from the fish

... The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is AJ699306. ...
ch_03_cells_presentation
ch_03_cells_presentation

... • Self-destruction of damaged cells • Lysosome membranes break down • Digestive enzymes released • Cell decomposes • Cellular materials recycle ...
The Cutting Edge of Affinity Electrophoresis Technology
The Cutting Edge of Affinity Electrophoresis Technology

... an SDS-PAGE gel that can specifically bind phosphorylated proteins migrating in the gel. This permits the separation of multiple phosphorylated forms of a protein, allowing quantitative analysis of the overall phosphorylation state and providing information on the differences in the functions of pro ...
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose

... Glycation is a process whereby sugar molecules form a covalent adduct with protein amino groups. In this study, we used ascorbic acid (AsA) as a glycating agent and purified cucumber (Cucumis sativus L.) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a model protein in chloroplast tiss ...
3-2 Organelles and the Cytoplasm
3-2 Organelles and the Cytoplasm

... • Self-destruction of damaged cells • Lysosome membranes break down • Digestive enzymes released • Cell decomposes • Cellular materials recycle ...
02 Cholesterol Metabolism2012-03-18 01:50617 KB
02 Cholesterol Metabolism2012-03-18 01:50617 KB

... transcription is suppressed and vice versa • Sterol Regulatory Element (SRE) is a recognition sequence in the DNA • SREBP (SRE binding protein) binding to SRE is essential for transcription of this gene • SREBP cleavage-activating protein (SCAP) is an intracellular cholesterol sensor ...
Presentazione di PowerPoint
Presentazione di PowerPoint

... 3.Transport: carrier proteins in the plasma membrane allow substances to enter or exit cells. ...
Insulin hormone: Mechanism and effects on the body and
Insulin hormone: Mechanism and effects on the body and

... transportation is realized without insulin, this glucose intake stage is controlled by insulin.52,54 Insulin receptors are expressed by astrocytes and neurons in the brain, like growing factors. Insulin enhances glucose uptake into astrocytes 55 but not in neurons. Neuronal insulin receptors are int ...
Amino acids - Zanichelli
Amino acids - Zanichelli

... 3.Transport: carrier proteins in the plasma membrane allow substances to enter or exit cells. ...
BCMB 3100 – Chapters 6,7,8 Enzyme Basics • Six Classes (IUBMB
BCMB 3100 – Chapters 6,7,8 Enzyme Basics • Six Classes (IUBMB

... • Affinity labels resemble substrates, but contain reactive groups to interact covalently with the enzyme ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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