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Supplementary Information
Supplementary Information

... for p53 are available for several p53 stretches and domains. However, a structure of the region between amino acids 61 and 93 is missing, probably due to higher residual dynamics. Since the results presented in this study favor the binding motif to Cyp18 to be localized exactly in this stretch, the ...
Stanford Presentation, 10/23/2001
Stanford Presentation, 10/23/2001

... and only then put the side chains on it. • The quality of the side chain modeling is therefore heavily dependent on the position of the backbone. If the initial backbone conformation is wrong, the side chain modeling quality will be accordingly bad. • What is really needed is a “combined” algorithm ...
Ion Exchange Chromatography
Ion Exchange Chromatography

... The analytes of interest must then be detected by some means, typically by conductivity or UV/Visible light absorbance. A chromatography data system (CDS) is usually needed to control an IC. ...
Protein Use during Training
Protein Use during Training

... intake with the thought that the additional protein will help build strength and power. Will protein help aid performance? Some studies suggest that protein does, in fact, help aid performance if utilized correctly. This article will explore the proper quantities of protein and dosing patterns neede ...
Sterilization & Disinfection
Sterilization & Disinfection

... sterilization of heat-sensitive materials such as surgical instruments and plastics. * It kills by alkylating both proteins and nucleic acids; i.e., the hydroxyethyl group attacks the reactive hydrogen atoms on essential amino and hydroxyl groups. ...
24.8 Fates of the Carbon Atoms from Amino Acids
24.8 Fates of the Carbon Atoms from Amino Acids

... We can classify the amino acids according to the number of carbon atoms. • Amino acids that provide three-carbon compounds are converted to pyruvate. • Amino acids with four carbon atoms are converted to oxaloacetate. • Five-carbon amino acids provide α-ketoglutarate. Some amino acids are listed twi ...
1 - El Camino College
1 - El Camino College

... 82. Fats are found solid at room temperature depending on the amount of: a. nitrogens b. lipids c. phosphate d. hydrogenes 83. Hemoglobin and antibodies are samples of: a. lipids b. protein c. carbohydrate d. nucleic acids Match the following molecules with the cellular process or processes in which ...
Chemotropism of Achlya ambisexualis to Methionine
Chemotropism of Achlya ambisexualis to Methionine

... Table 1 shows the results obtained when a variety of substances were tested for chemotropic activity in A. ambisexualis. Among individual amino acids only L-methionine induced a tropic response (Table 1a). The reorientation of hyphal tips (Fig. 1d) in response to changed positions of methionine-cont ...
1. The system: peptide class I MHC complexes
1. The system: peptide class I MHC complexes

... 3.b. Peptide dynamics and immune reactivity While addressing the role of conformational ensembles vs. induced fit is important, the issue of peptide dynamics in the MHC binding groove also speaks to the function of the peptide/MHC complex. If the peptide populates different conformations, it could b ...
1 A Comparative, Double-blind, Triple Crossover Net Nitrogen
1 A Comparative, Double-blind, Triple Crossover Net Nitrogen

... The UAA and the UCA of an specific protein can be determined through the N balance in a subject while that particular protein is given. To better understand UAA and UCA are, in relation to an specific protein, it is necessary to analyze the following: When a protein is digested, namely, it is enzima ...
Oxidative Phosphorylation
Oxidative Phosphorylation

... Catabolic & Anabolic Reactions ...
Key To Problem Set 3R
Key To Problem Set 3R

... A-3. The enzyme probably cuts (on the amino side of a stop transfer sequence) . Explanation: The protein enters the ER using its SP on the amino end. If the SP were the only transmembrane segment, the entire protein would be inside the lumen (except for the SP) and the protein would not be a transme ...
Chapter 7 Quiz Review
Chapter 7 Quiz Review

... Peptide bonds ...
handout nucleic acids and DNA replication
handout nucleic acids and DNA replication

... structure is identical in all DNA molecules. The only part of DNA that varies between different molecules is the base sequence. Therefore, the sequence of bases in DNA must determine the sequence of amino acids in a polypeptide chain. The length of DNA that codes for a polypeptide chain is called a ...
8/27/08 Transcript I
8/27/08 Transcript I

...  Simply means the product of one enzyme catalyzed reaction is furnished to another enzyme. It doesn’t diffuse into the bulk solution, it is furnished nanometers away from the active site.  You go from one active site to another active site without the products diffused into the bulk solution.  Yo ...
Aim of the lab - Institute of Microelectronics
Aim of the lab - Institute of Microelectronics

...  Applications of ferrofluids in medicine. Two case studies: 1) Hyperthermia and 2) Contrast Agents for Magnetic Resonance Imaging (MRI) Two applications of ferrofluids in medicine will be discussed:  Hyperthermia covers a wide variety of techniques in which elevation of temperature in ferrofluids ...
The Structure and Function of Large Biological Molecules
The Structure and Function of Large Biological Molecules

Protein Structure Analysis - G
Protein Structure Analysis - G

... A reducing reagent may be used to break disulfide bonds within protein molecules or between two or more polypeptide chains. By making a selective use of reducing agents it is possible determine structure of biologically active protein molecules or complexes. In this lab activity, students study the ...
THE SCIENTIFIC METHOD Define problem Research and collect
THE SCIENTIFIC METHOD Define problem Research and collect

... The shape, size, and orientation determine the function. Two important properties of an atom: charge and mass Protons – positive charge, located in nucleus Electrons – negative charge, located in electron shells Neutrons – neutral positive, located in nucleus Nucleus of an atom – positively charged, ...
Genetics Protein Project
Genetics Protein Project

... Human myoglobin has 153 amino acid residues in a highly folded and compact structure with eight separate and distinct alpha helical secondary structures. ...
Reading Guide for Week 4
Reading Guide for Week 4

... precursor metabolites, but you should understand that the central metabolic pathways produce them and that they are used to make subunits: Subunits (made from precursor metabolites) amino acids nucleotides monosaccharides glycerol fatty acids ...
Effects of Anions on the Positive Ion Electrospray Ionization Mass
Effects of Anions on the Positive Ion Electrospray Ionization Mass

Outline06 Metabolism - Napa Valley College
Outline06 Metabolism - Napa Valley College

... a. Hydrolysis of triglycerides triglyceride + H2O → fatty acids + glycerol b. Beta oxidation - stepwise oxidation of fatty acids - fatty acids are broken down into 2C units → acetyl CoA → Krebs Cycle → CO2 + H2O - high energy yield: >100 ATP per fatty acid > 2X more energy yield per gram than carboh ...
Study of Enzyme Mechanisms
Study of Enzyme Mechanisms

... • In the absence of EF, hydrolysis of NAD+ will occur – Model the TS & understand how stabilization of TS occurs – Occurs via an SN2 mechanism! O NH2 ...
101 -- 2006
101 -- 2006

... __ 46. What is the general process of breaking down large molecules into smaller ones called? a) catalysis b) metabolism c) anabolism d) dehydration e) catabolism __ 47. Photosynthesis is exergonic. a) True b) False __ 48. Which of the following statements is true concerning catabolic pathways? a) ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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