Receptors and Hormone Action
... z E.g. Protein kinase A activate cAMP-responsiveelement binding protein (CREB), or modify structural proteins in chromatin z Activated CREB binds to specific cAMP-responsive elements in the regulatory regions of certain genes to activate gene expression ...
... z E.g. Protein kinase A activate cAMP-responsiveelement binding protein (CREB), or modify structural proteins in chromatin z Activated CREB binds to specific cAMP-responsive elements in the regulatory regions of certain genes to activate gene expression ...
Thermal unfolding of proteins at high pH range studied by UV
... protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used ...
... protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In this paper we measured the degree of ionization of tyrosine residues and used ...
Identification of cellular proteins that bind to the human
... as affinity reagents. Myr, Myristic acid residue; R, arginine; H, histidine. ...
... as affinity reagents. Myr, Myristic acid residue; R, arginine; H, histidine. ...
Chapter 3—The Cell I. Cell Theory. a. Organisms are made of 1 or
... phosphate = PO4-3) to ADP to form ATP. When ATP releases a phosphate group, it becomes ADP. (ATP ADP + Pi). j. Oxidation-reduction reactions (Redox). i. When a molecule or substance gives up one or more electrons, it is said to be "oxidized." ii. When a molecule or substance accepts electrons, it ...
... phosphate = PO4-3) to ADP to form ATP. When ATP releases a phosphate group, it becomes ADP. (ATP ADP + Pi). j. Oxidation-reduction reactions (Redox). i. When a molecule or substance gives up one or more electrons, it is said to be "oxidized." ii. When a molecule or substance accepts electrons, it ...
Probability-Based Scoring Function as a Software
... the “Hits” protein results from EMBOSS, DTASelect, Solomon, Sillero, Rodwell and Wikipedia databases as shown in the dashed box in Fig. (2). In order to rank the ‘Hits’ protein results, five criteria were considered consecutively, (i) probability-based score, (ii) number of matched proteins, (iii) p ...
... the “Hits” protein results from EMBOSS, DTASelect, Solomon, Sillero, Rodwell and Wikipedia databases as shown in the dashed box in Fig. (2). In order to rank the ‘Hits’ protein results, five criteria were considered consecutively, (i) probability-based score, (ii) number of matched proteins, (iii) p ...
Amino Acid Biosynthesis
... – Synthesis of most amino acids is only one or two steps removed from another pathway or cycle. – Missing genes = metabolites not produced. Many metabolites are not essential to the cell. ...
... – Synthesis of most amino acids is only one or two steps removed from another pathway or cycle. – Missing genes = metabolites not produced. Many metabolites are not essential to the cell. ...
Central Dogma
... flash.htm http://highered.mcgrawhill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/si tes/dl/free/0072437316/120077/micro06.swf::Protein Synthesis ...
... flash.htm http://highered.mcgrawhill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/si tes/dl/free/0072437316/120077/micro06.swf::Protein Synthesis ...
GYP5 - Genetics
... shown to be a GAP for Ypt1p (DE ANTONI et al. 2002), a Rab GTPase involved in ER to Golgi trafficking (BACON et al. 1989, SEGEV, 1991; for review see LAZAR et al. 1997). Gyl1p is a protein with sequence similarity to Gyp5 and has been shown to colocalize with Gyp5p (CHESNEAU et al. 2004). We show th ...
... shown to be a GAP for Ypt1p (DE ANTONI et al. 2002), a Rab GTPase involved in ER to Golgi trafficking (BACON et al. 1989, SEGEV, 1991; for review see LAZAR et al. 1997). Gyl1p is a protein with sequence similarity to Gyp5 and has been shown to colocalize with Gyp5p (CHESNEAU et al. 2004). We show th ...
Free tyrosine and tyrosine-rich peptide
... and aromatic monoamines known to behave as precursors and/or analogs of catecholamine-type neurotransmitters highly rich in brains. Based on the peroxidative model redox mechanism [9] in which Cu-bound forms of PrP catalyzes the generation of ROS in the presence of some natural co-factors and induce ...
... and aromatic monoamines known to behave as precursors and/or analogs of catecholamine-type neurotransmitters highly rich in brains. Based on the peroxidative model redox mechanism [9] in which Cu-bound forms of PrP catalyzes the generation of ROS in the presence of some natural co-factors and induce ...
Atherosclerosis
... summarized by Hearse in 1979 and are still accurate today. The initial events occur within the few seconds or minutes after total coronary artery occlusion and are associated with reversible changes. This is characterized by the lack of oxygen delivery, and a reduced production of energy stores (ATP ...
... summarized by Hearse in 1979 and are still accurate today. The initial events occur within the few seconds or minutes after total coronary artery occlusion and are associated with reversible changes. This is characterized by the lack of oxygen delivery, and a reduced production of energy stores (ATP ...
Chemical Aspects of Life.
... Most cells are 70-90% water, the bulk of then dry weight consists of carbon (C), hydrogen (H) oxygen (0), nitrogen eN), and phosphorus (P), variously arranged into four major types of organic compounds: carbohydrates, lipids, proteins, and nu cleic acids. The more complex members of these categorie ...
... Most cells are 70-90% water, the bulk of then dry weight consists of carbon (C), hydrogen (H) oxygen (0), nitrogen eN), and phosphorus (P), variously arranged into four major types of organic compounds: carbohydrates, lipids, proteins, and nu cleic acids. The more complex members of these categorie ...
Homology Modeling via Protein Threading - lmm
... The enclosing mintЄT ensures that the lower bound will be instantiated on a specific legal threading tlbЄT. This will be used in splitting T, below. The equation further ensures that the singleton term, in g1(i, ti ), remains consistent both with the terms that reflect loop scores, in g2(i - 1, i, t ...
... The enclosing mintЄT ensures that the lower bound will be instantiated on a specific legal threading tlbЄT. This will be used in splitting T, below. The equation further ensures that the singleton term, in g1(i, ti ), remains consistent both with the terms that reflect loop scores, in g2(i - 1, i, t ...
Alpha-COPI Coatomer Protein Is Required for Rough Endoplasmic
... and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDELcontaining proteins were shown to have altered expression levels. LC-MS/MS mass spectrometry was used to analyze midgut microsomal proteins isolated from unfed and amino acid fed m ...
... and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDELcontaining proteins were shown to have altered expression levels. LC-MS/MS mass spectrometry was used to analyze midgut microsomal proteins isolated from unfed and amino acid fed m ...
Extended Project Description
... Background: The human genome contains over 500 homologous protein kinases, which control cellular signalling processes. They have become one of the most important target classes for the design of therapeutic inhibitors; many diseases are caused by dysregulation of cellular signalling processes. Most ...
... Background: The human genome contains over 500 homologous protein kinases, which control cellular signalling processes. They have become one of the most important target classes for the design of therapeutic inhibitors; many diseases are caused by dysregulation of cellular signalling processes. Most ...
Activity 3
... pH 2.0 Pepsin obtains optimal activity in a highly acidic medium (pH 1.8-3.5). When pH exceeds 5.0, very little or no proteolytic activity will take ...
... pH 2.0 Pepsin obtains optimal activity in a highly acidic medium (pH 1.8-3.5). When pH exceeds 5.0, very little or no proteolytic activity will take ...
Lecture 27
... N-acetylglutamate is synthesized from glutamate and acetylCoA by N-acetylglutamate synthase, it is hydrolyzed by a specific hydrolase. Rate of urea production is dependent on [N-acetylglutamate]. When aa breakdown rates increase, excess nitrogen must be excreted. This results in increase in Glu thro ...
... N-acetylglutamate is synthesized from glutamate and acetylCoA by N-acetylglutamate synthase, it is hydrolyzed by a specific hydrolase. Rate of urea production is dependent on [N-acetylglutamate]. When aa breakdown rates increase, excess nitrogen must be excreted. This results in increase in Glu thro ...
Problem Set 3
... Mutate and Auto Fit. You lose the opportunity to Reject the fit of the mutated amino acid, but you can always start over with Delete to remove the new amino acid completely. The fourth amino acid in the loop you are rebuilding is Arg, and I’ve found repeatedly that this amino acid is not placed very ...
... Mutate and Auto Fit. You lose the opportunity to Reject the fit of the mutated amino acid, but you can always start over with Delete to remove the new amino acid completely. The fourth amino acid in the loop you are rebuilding is Arg, and I’ve found repeatedly that this amino acid is not placed very ...
Materials and Methods
... Analyses of sequence parameters influencing suitability for NMR structure determination. Nearly 1,000 protein constructs less than 200 amino acids in length that were expressed and soluble at a sufficient level were screened for their suitability for NMR solution-structure determination by the NESG. ...
... Analyses of sequence parameters influencing suitability for NMR structure determination. Nearly 1,000 protein constructs less than 200 amino acids in length that were expressed and soluble at a sufficient level were screened for their suitability for NMR solution-structure determination by the NESG. ...
Document
... • example: hydration of a cis alkene (but not its trans isomer) to give an R alcohol (but not the S alcohol) ...
... • example: hydration of a cis alkene (but not its trans isomer) to give an R alcohol (but not the S alcohol) ...
Separation of nuclear protein complexes by blue native
... whole cell lysates [8]. Moreover, an adaptation of the technique to agarose gel matrices with a higher separation limit was utilized for separation of very large protein complexes such as pyruvate dehydrogenase complex [4] or virus particles [9]. The procedure has several advantages that make the BN ...
... whole cell lysates [8]. Moreover, an adaptation of the technique to agarose gel matrices with a higher separation limit was utilized for separation of very large protein complexes such as pyruvate dehydrogenase complex [4] or virus particles [9]. The procedure has several advantages that make the BN ...
Transferase-catalyses transfer of a group from one molecule to
... first. The phenomenon of one active site being activated by an event happening elsewhere in the enzyme is called allosteric activation. So enzymes subunits cooperate each other to cause the full activation of the enzyme. In order to start this activation process certain concentration of substrate i ...
... first. The phenomenon of one active site being activated by an event happening elsewhere in the enzyme is called allosteric activation. So enzymes subunits cooperate each other to cause the full activation of the enzyme. In order to start this activation process certain concentration of substrate i ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.