Unraveling the mechanisms of RNA
... cytoplasm, regulation of RNA stability and translation [1]. These interactions are highly dynamic and are critical in the regulation of the various steps involved in RNA processing. In addition, these individual RNA-protein interactions occur in the environment of extremely large, multi-component nu ...
... cytoplasm, regulation of RNA stability and translation [1]. These interactions are highly dynamic and are critical in the regulation of the various steps involved in RNA processing. In addition, these individual RNA-protein interactions occur in the environment of extremely large, multi-component nu ...
The HSP90 family of genes in the human genome
... Introduction HSP90 proteins, named according to the 90-kDa average molecular mass of their members, are highly conserved molecular chaperones that account for 1 – 2% of all cellular proteins in most cells under non-stress conditions [1]. HSP90 proteins have key roles in signal transduction, protein ...
... Introduction HSP90 proteins, named according to the 90-kDa average molecular mass of their members, are highly conserved molecular chaperones that account for 1 – 2% of all cellular proteins in most cells under non-stress conditions [1]. HSP90 proteins have key roles in signal transduction, protein ...
1 course syllabus bio 1023 - introductory nutrition
... of competency are established by the Biology faculty as Departmental policy. ...
... of competency are established by the Biology faculty as Departmental policy. ...
Consortium for Educational Communication
... The term ‘enzyme’ was introduced by Kuhne in 1878. It is taken from the Greek word Zume (Zyme) meaning ‘Leaven’. Enzymes are the principles found in Leaven. The first observation was carried out on vegetable and animal cell free extracts. Berzilius in 1836 coined the term catalysis which in Greek me ...
... The term ‘enzyme’ was introduced by Kuhne in 1878. It is taken from the Greek word Zume (Zyme) meaning ‘Leaven’. Enzymes are the principles found in Leaven. The first observation was carried out on vegetable and animal cell free extracts. Berzilius in 1836 coined the term catalysis which in Greek me ...
Computational Biophysics
... Conformational stability is a critical property of the collagen triple helix. It is in part ensured by the presence of a repetitive GXY sequence motif, with a high frequency of proline residues in the X position and hydroxyproline residues in the Y position. A high natural abundance of lysine residu ...
... Conformational stability is a critical property of the collagen triple helix. It is in part ensured by the presence of a repetitive GXY sequence motif, with a high frequency of proline residues in the X position and hydroxyproline residues in the Y position. A high natural abundance of lysine residu ...
Integration and regulation of fuel metabolism in maintaining
... activation or inhibition of many enzymes, with anabolic actions being accentuated(glycogenesis, lipogenesis, and protein synthesis, Table 1). (3) One slower action of insulin, occurring in minutes to several hours, involves a further regulation of enzyme activity through the selective induction (glu ...
... activation or inhibition of many enzymes, with anabolic actions being accentuated(glycogenesis, lipogenesis, and protein synthesis, Table 1). (3) One slower action of insulin, occurring in minutes to several hours, involves a further regulation of enzyme activity through the selective induction (glu ...
Membrane protein integration into the endoplasmic reticulum
... of them containing charged residues [49–51]. These observations, together with the fact that TRAM itself contains an unusually high number of charged residues within its TM segments, led to the idea that TRAM could act as a chaperone for the integration of nonoptimal TM segments by providing a more ...
... of them containing charged residues [49–51]. These observations, together with the fact that TRAM itself contains an unusually high number of charged residues within its TM segments, led to the idea that TRAM could act as a chaperone for the integration of nonoptimal TM segments by providing a more ...
Caspase Colorimetric Protease Assay Sampler Kit (Caspases-2,
... Apoptosis, or programmed cell death, is the outcome of a programmed intracellular cascade of genetically determined steps. One of the mechanisms which is consistently implicated in apoptosis is the activation of a group of intracellular cysteine proteases called caspases. Caspases play a critical ro ...
... Apoptosis, or programmed cell death, is the outcome of a programmed intracellular cascade of genetically determined steps. One of the mechanisms which is consistently implicated in apoptosis is the activation of a group of intracellular cysteine proteases called caspases. Caspases play a critical ro ...
BISC-576 Practical Statistics and Bioinformatics Instructors:
... will introduce alpha-helices and beta-sheets and the Ramachandran plot as means of identifying secondary structure elements of proteins. In addition, the basic principles for structural alignment methods will be discussed and various proteins will be aligned. We will classify protein folds according ...
... will introduce alpha-helices and beta-sheets and the Ramachandran plot as means of identifying secondary structure elements of proteins. In addition, the basic principles for structural alignment methods will be discussed and various proteins will be aligned. We will classify protein folds according ...
Binding Protein HU has a Regulatory Role in the Acid Stress
... cells overexpress a set of ~49 proteins when it is subjected to an acid stress. Among those proteins, we have identified the urease subunits UreA and UreB, Pfr, NapA, and the DNA-binding protein HU [9,10]. We have also shown that the Fur-regulated (ferric uptake regulator) arginine decarboxylase gen ...
... cells overexpress a set of ~49 proteins when it is subjected to an acid stress. Among those proteins, we have identified the urease subunits UreA and UreB, Pfr, NapA, and the DNA-binding protein HU [9,10]. We have also shown that the Fur-regulated (ferric uptake regulator) arginine decarboxylase gen ...
Chapter 10
... into the endoplasmic reticulum where protein folding and processing take place. From the endoplasmic reticulum, proteins are transported in vesicles to the Golgi apparatus where they are further processed and sorted for transport to endosomes, lysosomes, the plasma membrane, or secretion from the ce ...
... into the endoplasmic reticulum where protein folding and processing take place. From the endoplasmic reticulum, proteins are transported in vesicles to the Golgi apparatus where they are further processed and sorted for transport to endosomes, lysosomes, the plasma membrane, or secretion from the ce ...
Selective Dimerization of a C2H2 Zinc Finger Subfamily
... C-terminal fingers. To examine this interaction in greater detail, a smaller protein was prepared that contains only 79 amino acids from the N terminus of IK I (NTS) fused to a 64 amino acid region containing the C-terminal zinc fingers. A FLAG epitope tag was included at the N terminus. This 18 kDa ...
... C-terminal fingers. To examine this interaction in greater detail, a smaller protein was prepared that contains only 79 amino acids from the N terminus of IK I (NTS) fused to a 64 amino acid region containing the C-terminal zinc fingers. A FLAG epitope tag was included at the N terminus. This 18 kDa ...
Onset of lactation in the bovine mammary gland:
... after parturition in dairy cow, the mammary gland undergoes dramatic functional and metabolic changes for lactogenesis. Lactogenesis, the initiation of milk synthesis and secretion, includes two stages (Neville et al. 2002). Stage I begins a few weeks before parturition and is characterized by mamma ...
... after parturition in dairy cow, the mammary gland undergoes dramatic functional and metabolic changes for lactogenesis. Lactogenesis, the initiation of milk synthesis and secretion, includes two stages (Neville et al. 2002). Stage I begins a few weeks before parturition and is characterized by mamma ...
Launch Activity
... this is very soluble and toxic, so is not around for long! It is then combined with CO2 using ATP to produce UREA (CO(NH2)2 this occurs in the ornithine cycle. ...
... this is very soluble and toxic, so is not around for long! It is then combined with CO2 using ATP to produce UREA (CO(NH2)2 this occurs in the ornithine cycle. ...
A Drosophila Third Chromosome Minute Locus Encodes
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
N-terminus of M2 protein could induce antibodies with inhibitory
... virus infection are still a subject of research of many investigators. The trivalent inactivated vaccines, which contain the hemagglutinin (HA) of in£uenza A (H1N1), in£uenza A (H3N2) and in£uenza B, are the only licensed commercial vaccines [1]. Because of the high mutation rate of HA, we should be ...
... virus infection are still a subject of research of many investigators. The trivalent inactivated vaccines, which contain the hemagglutinin (HA) of in£uenza A (H1N1), in£uenza A (H3N2) and in£uenza B, are the only licensed commercial vaccines [1]. Because of the high mutation rate of HA, we should be ...
PDF + SI - GenScript
... To further explore the notion that a discrete 11-member CMG complex exists, we embarked on independent purification methods. We prepared 0- to 12-h embryo extracts from Drosophila expressing a functional, FLAG-tagged version of Mcm6 (32). A single-step affinity purification of FLAG-Mcm6 from these f ...
... To further explore the notion that a discrete 11-member CMG complex exists, we embarked on independent purification methods. We prepared 0- to 12-h embryo extracts from Drosophila expressing a functional, FLAG-tagged version of Mcm6 (32). A single-step affinity purification of FLAG-Mcm6 from these f ...
Aminoaciduria
... Early diagnosis of PKU is important because the disease is treatable by dietary means. Newborn with PKU frequently has normal blood levels of phenylalanine (PA) at birth As the mother clears increased blood PA in her fetus through placenta. So, test performed at birth may show false –ve results. PA ...
... Early diagnosis of PKU is important because the disease is treatable by dietary means. Newborn with PKU frequently has normal blood levels of phenylalanine (PA) at birth As the mother clears increased blood PA in her fetus through placenta. So, test performed at birth may show false –ve results. PA ...
Effects of Protein-Deprivation on the Regeneration of Rat Liver after
... with lO,Ci of L-[4,5-3H]leucine (47Ci/mmol, New England Nuclear). Mixed liver proteins were isolated as described below for the protein-degradation experiments. Protein content was determined by the method of Lowry et al. (1951) on a separate sample. The results were calculated by taking into consid ...
... with lO,Ci of L-[4,5-3H]leucine (47Ci/mmol, New England Nuclear). Mixed liver proteins were isolated as described below for the protein-degradation experiments. Protein content was determined by the method of Lowry et al. (1951) on a separate sample. The results were calculated by taking into consid ...
What is Ketosis
... Insufficient insulin production (Type I) Ineffective or impaired insulin function (Type II) ...
... Insufficient insulin production (Type I) Ineffective or impaired insulin function (Type II) ...
Chapter Sixteen - Wright State University
... • Insulin is a hormone that regulates blood glucose levels. • Insulin contains a single strand of 51 amino acids; it’s primary a.a. sequence was first determined in 1953 by Frederick Sanger. It took 8 years to work this out, and Sanger received a Nobel Prize for his work. • Today amino acid sequence ...
... • Insulin is a hormone that regulates blood glucose levels. • Insulin contains a single strand of 51 amino acids; it’s primary a.a. sequence was first determined in 1953 by Frederick Sanger. It took 8 years to work this out, and Sanger received a Nobel Prize for his work. • Today amino acid sequence ...
The Effect of Actidione and other Antifungal Agents on Nucleic Acid
... Preliminary investigations. The manometric techniques of Umbreit, Burris & Stauffer (1949) were used to study the effect of actidione on oxygen consumption and anaerobic production of carbon dioxide by Saccharornyces carlsbergensis in the presence of glucose. Actidione at concentrations up to 1 mg./ ...
... Preliminary investigations. The manometric techniques of Umbreit, Burris & Stauffer (1949) were used to study the effect of actidione on oxygen consumption and anaerobic production of carbon dioxide by Saccharornyces carlsbergensis in the presence of glucose. Actidione at concentrations up to 1 mg./ ...
14 Alignment 3(1)
... line contains information on the number of identical residues in this alignment (Identities), the number of conservative substitutions (Positives), and if applicable, the number of gaps in the alignment. Finally, the actual alignment is shown, with the query on top, and the database match is labeled ...
... line contains information on the number of identical residues in this alignment (Identities), the number of conservative substitutions (Positives), and if applicable, the number of gaps in the alignment. Finally, the actual alignment is shown, with the query on top, and the database match is labeled ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.