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Journal of Applied Microbiology
Journal of Applied Microbiology

... Methods and Results: Two Aer. veronii mucin- and lactoferrin-binding proteins with molecular masses of 37 and 48 kDa were identified by Western blot analysis. According to its N-terminal amino acid sequence, the 48-kDa protein was identified as Omp48, an outer-membrane protein similar to LamB of Esc ...
Final Key - UC Davis Plant Sciences
Final Key - UC Davis Plant Sciences

... F6P and GA3P by the pentose phosphate pathway. Thus, activation of glycolysis by xylulose via PFK-2 will metabolize excess dietary xylulose. ...
Aromatic amino acid metabolism
Aromatic amino acid metabolism

... adequate to support growth. Tyrosine, an essential nutrient for individuals with phenylketonuria, must be supplied in the diet. ...
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families

... Liu and Califano have recently created a program, CASTOR, which uses statistically significant motifs to identify protein regions likely to have functional significance and then classifies proteins within a family based on these regions (Liu and Califano 2003). This program differs from many similar ...
Nutritional value of wheat for livestock
Nutritional value of wheat for livestock

... there may be something unusual about the poorly digested starch of wheat grains. A component of wheat called ‘non-starch polysaccharide’ (NSP) has been implicated in the poor starch digestibility and low AME. A laboratory assay is currently being developed which aims to separate ‘good’ (high AME) fr ...
No Slide Title
No Slide Title

... Fold classification based on structure-structure alignment of proteins (FSSP) FSSP is based on a comprehensive comparison of PDB proteins (greater than 30 amino acids in length). Representative sets exclude sequence homologs sharing > 25% amino acid identity. The output includes a “fold tree.” ...
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Lecture 18

... they undergo microbial fermentation produce volatile fatty acids (VFA): e.g., acetic, propionic, butyric etc. The vast majority of VFAs are passively absorbed through the rumen wall. (So while the saliva buffers the pH up, digestion is producing acids moving the pH down; absorption of acids of cours ...
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica

... Chapter 4 Results ........................................................................................................................... 33  4.1  IHF is not an RdoA target .................................................................................................... 33  4.2  Comparison of ...
characterisation of novel proteins
characterisation of novel proteins

... 383 amino acids in length respectively. Although these genes were originally isolated from B. thuringiensis, the DNA sequences of these two genes have been modified in order to alter the guanosine and cytosine codon bias to a level more typical for plant codons. The deduced amino acid sequences of t ...
Cloning and characterization of the
Cloning and characterization of the

... is shown in Figure 2B, while Figure 2C shows the quantitation of all the results as the molar amount of pseudouridine formed per mole of tRNA, in the presence of increasing amounts of recombinant scPus1p or spPus1p and using as substrates the tRNAs mentioned above. Control experiments with yeast tRN ...
4. DIGESTION AND ABSORPTION OF LIPIDS
4. DIGESTION AND ABSORPTION OF LIPIDS

... • This is necessary for their absorption, since the cells lining the intestine are able to absorb them into the bloodstream only as relatively small molecules. ...
33_organelles.txt 3/25/2010 Limited proteolysis, phosphorylation
33_organelles.txt 3/25/2010 Limited proteolysis, phosphorylation

... contains 37 known genes, most coding for tRNA's. FTP, name this genetic material generally inherited only for the mother; a ribonucleic acid present in the "power plant" of cells. Answer: mitochondrial deoxyribonucleic acid (prompt on deoxyribonucleic acid, mitochondria or mitochondrion) A prolonged ...
2-Oxoacid dehydrogenase multienzyme complexes
2-Oxoacid dehydrogenase multienzyme complexes

Test File
Test File

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Enzymes of the Calvin Cycle and Intermediary

... gluconate dehydrogenase activity increased approximately 2-fold in both light- and C02limited cultures with decreasing dilution rate, the minimum activity in C0,-limited cultures [25 nmol substrate converted min-l (mg protein)-l] was 2.5 times greater than the lowest activity in light-limited cultur ...
HIV-1 Vpr-chloramphenicol acetyltransferase fusion proteins
HIV-1 Vpr-chloramphenicol acetyltransferase fusion proteins

... 88CAT, were specifically detected in the corresponding cell lysates (Figure 1b, upper left panel, lanes 2–8). The molecular weight of the different Vpr-CAT fusion proteins ranged between approximately 29 kDa and 35 kDa. Analysis of viral lysates indicates that Vpr-CAT fusion proteins R1-42CAT, R1-80 ...
Regulation of carbohydrate metabolism
Regulation of carbohydrate metabolism

... 1. 3 key enzymes for the regulation of glycolysis (their activation). The role of Fructose 2,6-P in the regulation of glycolysis and gluconeogenesis. 2. 3 key sites for the regulation of gluconeogenesis (their activation). 3. The signal pathway for the activation of glycogen degradation by glucagon. ...
- humans ingest more proteins than needed for replacement of
- humans ingest more proteins than needed for replacement of

... precursor in liver for synthesis of glucose (glycogenic or gluconeogenic) - some amino acids (try, phe) only part of their carbon skeleton can be used to synthesize glucose because the remainder is converted to acetyl CoA that cannot be used in synthesis of glucose - leucine is solely ketogenic  it ...
Mitochondrial dysfunction in neurodevelopmental disorders
Mitochondrial dysfunction in neurodevelopmental disorders

... Mitochondrial fusion and fission events are required for the maintenance of a healthy mitochondrial population (beige). Mitochondrial fusion is thought to facilitate the interchange of internal components such as copies of the mitochondrial genome, respiratory proteins and metabolic products. Mitoch ...
Spring 97, Exam 1
Spring 97, Exam 1

... You have 80 minutes for this exam. Exams written in pencil or erasable ink will not be re-graded under any circumstances. Some information which may be useful is provided on the bottom half of the next page. Explanations should be concise, a couple of sentences. You will need a calculator for this e ...
The Bacterial Cell Wall
The Bacterial Cell Wall

... The emergence of many antibiotic-resistant strains of once-sensitive bacteria is a major theme of current research and scientific literature, and is regularly publicised in the media. Aims of the course: Review the molecules and structures of the cell envelope of bacterial cells and describe the rol ...
Small-molecule metabolism: an enzyme mosaic
Small-molecule metabolism: an enzyme mosaic

... 722 domains in 213 families in 510 (88%) of the E. coli small-molecule metabolism (SMM) enzymes (summarized in Box 2 and Table 1). There are, on average, 3.4 domains per family, which shows that even this basic set of pathways is the product of extensive duplication of domains within its enzymes. Th ...
Topic 3 Proteins as Drug Targets
Topic 3 Proteins as Drug Targets

... Intermolecular bonds are involved in binding No reaction takes place on the inhibitor Inhibition depends on the strength of inhibitor binding and inhibitor concentration Substrate is blocked from the active site Increasing substrate concentration reverses inhibition Inhibitor likely to be similar in ...
Fatty Acid Oxidation and Ketone Bodies OXIDATION OF FATTY
Fatty Acid Oxidation and Ketone Bodies OXIDATION OF FATTY

... Triacylglycerols constitute about 11 kg of his total body weight. If this amount were stored in glycogen, his total body weight would be 55 kg greater. ...
pdf-Dokument - Universität Bonn
pdf-Dokument - Universität Bonn

... This suggests that ARF1GAP play a regulator role towards ARF during Golgi apparatus to ER transport. ARFGAPs are generally considered a group of proteins, which stimulate the intrinsic GTPase activity of ARF proteins. However, an additional role has been suggested in the regulation of membrane traff ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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