Cloning and structure of three rainbow trout C3
... C3-1 apparently lacks Con A-binding carbohydrates in its a-chain, where an N-glycosylation site is predicted. Since Con A recognizes only high-mannose carbohydrates, the presence of carbohydrates that do not bind Con A cannot be excluded. Because the a-chain carbohydrate moiety in human C3 is involv ...
... C3-1 apparently lacks Con A-binding carbohydrates in its a-chain, where an N-glycosylation site is predicted. Since Con A recognizes only high-mannose carbohydrates, the presence of carbohydrates that do not bind Con A cannot be excluded. Because the a-chain carbohydrate moiety in human C3 is involv ...
SGKB
... specificity and catalysis, epitope mapping, cellular location, organ location Protein Networks and Biological Systems: enzymatic pathways and networks information Literature: synonyms for protein names, links to PubMed by database identifier and related text and authors Information from more than 50 ...
... specificity and catalysis, epitope mapping, cellular location, organ location Protein Networks and Biological Systems: enzymatic pathways and networks information Literature: synonyms for protein names, links to PubMed by database identifier and related text and authors Information from more than 50 ...
Methods for imaging and detecting modification of proteins
... primary components of the diet, biological membranes, and lipoproteins, and they are substrates for lipid peroxidation (LPO) [1–3]. They readily undergo nonenzymatic peroxidation during oxidative stress or are oxidized through reactions involving enzymes such as cyclooxygenase, cytochrome P450, or t ...
... primary components of the diet, biological membranes, and lipoproteins, and they are substrates for lipid peroxidation (LPO) [1–3]. They readily undergo nonenzymatic peroxidation during oxidative stress or are oxidized through reactions involving enzymes such as cyclooxygenase, cytochrome P450, or t ...
A nuclear matrix-associated high molecular mass nuclear antigen
... decreased substantially at 1.5 and 2.5 hours after release from the aphidicolin block at the G1/S boundary, but then increased again and reached a high level at 4.5 hours (Fig. 2B). Flow cytometry on PI-stained cells indicated that the 1.5- and 2.5 hour points corresponded to mid S and late S/G2 pha ...
... decreased substantially at 1.5 and 2.5 hours after release from the aphidicolin block at the G1/S boundary, but then increased again and reached a high level at 4.5 hours (Fig. 2B). Flow cytometry on PI-stained cells indicated that the 1.5- and 2.5 hour points corresponded to mid S and late S/G2 pha ...
Vol. 147, No. 3, 1987 September 30, 1987 BIOCHEMICAL AND
... preincubated at 30 ° C for 2 min; after I min aliquots of 15 lul were taken to determine the radioactivity bound unspecifically. At the second minute t~$ phosphorylation was~started by addition of 20 mMMgCl^ yielding 1.2 mM free Mgand 1.6 nM free Ca . The free metal ion concentratlons were calculate ...
... preincubated at 30 ° C for 2 min; after I min aliquots of 15 lul were taken to determine the radioactivity bound unspecifically. At the second minute t~$ phosphorylation was~started by addition of 20 mMMgCl^ yielding 1.2 mM free Mgand 1.6 nM free Ca . The free metal ion concentratlons were calculate ...
IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.
... The docking studies were performed on HVR protein (HIV-1 protease) and from the studies it was found that the compound SR-03[Resorcinol derivative (dock score -99.07 and hydrogen bond energy -7.17 KJ)] was found to have the greatest affinity to HVR protein (HIV-1 protease) followed by SR-02[Benzyl d ...
... The docking studies were performed on HVR protein (HIV-1 protease) and from the studies it was found that the compound SR-03[Resorcinol derivative (dock score -99.07 and hydrogen bond energy -7.17 KJ)] was found to have the greatest affinity to HVR protein (HIV-1 protease) followed by SR-02[Benzyl d ...
Protein Structure and Interactions
... Misfolded proteins, aggregates, and inclusion bodies are hallmarks of the cytopathology of neurodegenerative disorders including Huntington’s disease, Amyotropic lateral sclerosis, Parkinson’s disease, Prion diseases, and Alzheimer’s disease. The appearance of proteins with altered folded states is ...
... Misfolded proteins, aggregates, and inclusion bodies are hallmarks of the cytopathology of neurodegenerative disorders including Huntington’s disease, Amyotropic lateral sclerosis, Parkinson’s disease, Prion diseases, and Alzheimer’s disease. The appearance of proteins with altered folded states is ...
Full text
... containing 5 g l-1NH3, 0.5 g l-1 Triton-X, 0.5 g l-1 EDTA and 6 ml l-1 butan-1-ol according to Varian procedure was prepared by dilution with Milli-Q DI water. Inductively coupled plasma mass spectrometric measurements for multielement determination of trace elements were performed with a Varian 820 ...
... containing 5 g l-1NH3, 0.5 g l-1 Triton-X, 0.5 g l-1 EDTA and 6 ml l-1 butan-1-ol according to Varian procedure was prepared by dilution with Milli-Q DI water. Inductively coupled plasma mass spectrometric measurements for multielement determination of trace elements were performed with a Varian 820 ...
Systems-Wide Analysis of Acclimation Responses to Long
... Top-down systems biology approaches, where responses at multiple system levels are monitored over time and integrated to a more holistic picture, appear helpful to shed light on the many open questions regarding responses of plant cells to HS. In fact, Chlamydomonas is an ideal plant model for such ...
... Top-down systems biology approaches, where responses at multiple system levels are monitored over time and integrated to a more holistic picture, appear helpful to shed light on the many open questions regarding responses of plant cells to HS. In fact, Chlamydomonas is an ideal plant model for such ...
Expression and V (D) J recombination activity of mutated RAG
... deleted still retains its recombination function. In addition, a series of small deletions within the strongly conserved remaining 60% of the coding region was tested. Nine out of ten of these prove unable to provide RAG-1 activity, but one is quite active. Certain peptide sequences were also specif ...
... deleted still retains its recombination function. In addition, a series of small deletions within the strongly conserved remaining 60% of the coding region was tested. Nine out of ten of these prove unable to provide RAG-1 activity, but one is quite active. Certain peptide sequences were also specif ...
Derivation and testing of pair potentials for protein folding. When is
... chain compactness by estimating the expected side-chain contact probability by placing the sequence of interest in each member of a library of structures of comparable compactness to the native conformation. The side-chain contact maps are not allowed to readjust to the sequence of interest, i.e., t ...
... chain compactness by estimating the expected side-chain contact probability by placing the sequence of interest in each member of a library of structures of comparable compactness to the native conformation. The side-chain contact maps are not allowed to readjust to the sequence of interest, i.e., t ...
Whey Products - New Zealand Institute of Chemistry
... sulphate, phosphate, citrate, nitrate and other anions from the whey in return for OH- ions. When the cation exchange resin becomes saturated, it is regenerated ready for reuse: a solution of H2SO4 is passed through the resin bed so that the H+ ions present in high concentration displace the Ca2+, M ...
... sulphate, phosphate, citrate, nitrate and other anions from the whey in return for OH- ions. When the cation exchange resin becomes saturated, it is regenerated ready for reuse: a solution of H2SO4 is passed through the resin bed so that the H+ ions present in high concentration displace the Ca2+, M ...
Glycogen Metabolism
... is the fate of most of NH3 channeled there. Urea → bloodstream → kidneys → urine ...
... is the fate of most of NH3 channeled there. Urea → bloodstream → kidneys → urine ...
Amassin, an olfactomedin protein, mediates the massive
... the calculated molecular weight of 56.5 kD. The remaining molecular weight discrepancy could be due to other posttranslational modifications such as O-linked glycosylation. Structural analysis The amassin sequence is similar in the COOH-terminal half (residues 230–477) to a family of proteins that c ...
... the calculated molecular weight of 56.5 kD. The remaining molecular weight discrepancy could be due to other posttranslational modifications such as O-linked glycosylation. Structural analysis The amassin sequence is similar in the COOH-terminal half (residues 230–477) to a family of proteins that c ...
enzyme structure
... activities of enzymes are determined by their three-dimensional structure. However, although structure does determine function, predicting a novel enzyme's activity just from its structure is a very difficult problem that has not yet been solved. Most enzymes are much larger than the substrates they ...
... activities of enzymes are determined by their three-dimensional structure. However, although structure does determine function, predicting a novel enzyme's activity just from its structure is a very difficult problem that has not yet been solved. Most enzymes are much larger than the substrates they ...
Protein splicing of PRP8 mini-inteins from species of the genus
... The prp8 gene of three Penicillium species contains an intein To assess the occurrence of inteins in the prp8 gene of species of the genus Penicillium, putative intein-containing fragments, according to the insertion site of previously identified C. neoformans, A. nidulans, A. fumigatus, and H. caps ...
... The prp8 gene of three Penicillium species contains an intein To assess the occurrence of inteins in the prp8 gene of species of the genus Penicillium, putative intein-containing fragments, according to the insertion site of previously identified C. neoformans, A. nidulans, A. fumigatus, and H. caps ...
COMMUNICATION
... the presence of conserved regions. The greater similarity the leukocyte adhesion proteins, theRGD receptor a subbetween mouse and human Mac-1 (75%). thanbetween units are proteolytically cleaved during processing, and murine Mac-1 and LFA-1 (32%).or between human hence contain two disulfide-linked p ...
... the presence of conserved regions. The greater similarity the leukocyte adhesion proteins, theRGD receptor a subbetween mouse and human Mac-1 (75%). thanbetween units are proteolytically cleaved during processing, and murine Mac-1 and LFA-1 (32%).or between human hence contain two disulfide-linked p ...
Determination of Amino acids by UHPLC with automated
... described method, one destination vial is needed per sample. Vials 57 – 96 are configured as destination vials what means that vials 17 – 56 can be used as sample positions. At positions 57 – 96 the user has to place empty vials with sufficient volume as destination vials. They have to be closed wit ...
... described method, one destination vial is needed per sample. Vials 57 – 96 are configured as destination vials what means that vials 17 – 56 can be used as sample positions. At positions 57 – 96 the user has to place empty vials with sufficient volume as destination vials. They have to be closed wit ...
Henikoff, S. and Henikoff, Jorja G. Amino Acid Substitution Matrices from Protein Blocks. Proc. Natl. Acad. Sci. USA , 89, pp. 10915-10919, 1992.
... are aligned or realigned against sequence segments containing a candidate motif (16). The MOTOMAT program uses a substitution matrix whena block is extended to either side of the motif region and when scoring candidate blocks (10). A unitary substitution matrix (matches = 1; mismatches = 0) was used ...
... are aligned or realigned against sequence segments containing a candidate motif (16). The MOTOMAT program uses a substitution matrix whena block is extended to either side of the motif region and when scoring candidate blocks (10). A unitary substitution matrix (matches = 1; mismatches = 0) was used ...
CHAPTER 4 HF` Cleavage and Deprotection Procedures for
... On completion of chemical synthesis of the peptide chain, the final step requires the removal from the solid-phase support and liberation of the protected side chains of the trifunctional amino acids (I). Many different approaches to this problem have been established, but the procedure most widely ...
... On completion of chemical synthesis of the peptide chain, the final step requires the removal from the solid-phase support and liberation of the protected side chains of the trifunctional amino acids (I). Many different approaches to this problem have been established, but the procedure most widely ...
Three scientists who revealed the structure and workings of the
... Each ribosome can form 10 to 20 peptide bonds per second and has built-in error checking mechanisms to ensure that it only makes on average one mistake for every 100 000 amino acids added to a peptide chain. The whole process is orchestrated by a multitude of helper biomolecules that bind the riboso ...
... Each ribosome can form 10 to 20 peptide bonds per second and has built-in error checking mechanisms to ensure that it only makes on average one mistake for every 100 000 amino acids added to a peptide chain. The whole process is orchestrated by a multitude of helper biomolecules that bind the riboso ...
d05a1663be3edc4
... • SRP is rod-like; one end it binds ER SS another end bonds between the large and small ribosomal subunits blocks elongation, stoping translation; this ensures that the protein is not sent to the cytoplasm (this is VERY important for lysosomal enzymes as they can be deadly if the cell puts them in ...
... • SRP is rod-like; one end it binds ER SS another end bonds between the large and small ribosomal subunits blocks elongation, stoping translation; this ensures that the protein is not sent to the cytoplasm (this is VERY important for lysosomal enzymes as they can be deadly if the cell puts them in ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.