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Protein sequence analysis
Protein sequence analysis

... The filter option, if set to true, will allow you to mask out various segments of the query sequence for regions which are nonspecific for sequence similarity searches. Filtering can eliminate statistically significant but biologically uninteresting reports from the output, for example hits against ...
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Review article Zinc finger protein (ZFP) in plants
Review article Zinc finger protein (ZFP) in plants

... ZFP genes were only regulated in specific tissues or under specific stresses. For instance, LOC_Os01g07930 was just up-regulated in endosperm and 5d_seed. The LOC_ Os05g10670 was just up-regulated under drought and salt stresses. This suggests that these ZFP genes expressions with tissue-specific an ...
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macro-molecules
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... 6, 14, 20, 21, 27). All of these proteins bear large numbers of N-linked oligosaccharides and have apparent molecular. weights (determined by SDS-PAGE) in the range 80,000 to 120,000, whereas apparent molecular weights of the core polypeptides are in the range 40,000 to 60,000. The high level of gly ...
Document
Document

Glycerolipids and Glycerophospholipids
Glycerolipids and Glycerophospholipids

... • PI can be phosphorylated to different degrees • PIP2 = phosphatidylinositol 4,5-bisphosphate is very important in signal transduction – When a receptor G protein is activated it can mediate the cleavage of PIP2 to DG and IP3 – DG activates protein kinase C which adds phosphates to certain proteins ...
Cysteine 230 Modulates Tumor Necrosis Factor
Cysteine 230 Modulates Tumor Necrosis Factor

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... Initial selection of target mutant clones was performed using plasmid hydrolysis with both HindIII and BamHI endonucleases. Restriction analysis displayed a mutagenesis pattern characteristic for the method used [16]. A high yield (>96%) of plasmids with restored HindIII site was achieved in all exp ...
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... amino acids R and K in correlation with the G ‡ C content of the corresponding genome DNA, raised the possibility that local unevenness of genome DNA structure may be related to the tendency for repetitiveness of the genome (Nishizawa & Nishizawa, 1998). It has also been reported that eukaryotes, bu ...
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A Bifunctional tRNA for In Vitro Selection

... mRNA display [8, 9], covalent protein-mRNA fusions are formed on stalled translation complexes; the 3⬘ ends of mRNAs are modified in such a way that they stall protein synthesis, enter the A site of the ribosome, and act like an aminoacyl tRNA to become attached to their protein products. We have be ...
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CHAPTER 3 The Molecules of Cells
CHAPTER 3 The Molecules of Cells

... 3.8 Lipids include fats, which are mostly energystorage molecules • These compounds are composed largely of carbon and hydrogen – They are not true polymers ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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