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Distinct profiling of antimicrobial peptide families
Distinct profiling of antimicrobial peptide families

... into families and sub-families based on their primary sequences and structures (Kaiser and Diamond, 2000; Yeaman and Yount, 2003). However, there is substantial experimental evidence that even minor variations in peptide structures can lead to significant differences in AMP activities (Ganz, 2003; T ...
Defelipe, L.A, Dolghih E, Roitberg A.E., Nouzova M., Mayoral
Defelipe, L.A, Dolghih E, Roitberg A.E., Nouzova M., Mayoral

... in a hydrophobic pocket formed by Ile-151, Ile-154, Tyr 155, Leu158, Val-221 and Val-224 (Fig. 4A). Docking results indicated a clear difference in the interaction of (10R)-JHA and (10S)-JHA with the hydrophobic pocket of the binding site of AeJHAMT. Specifically, while the epoxide ring of (10S)-JHA ...
Introduction
Introduction

... by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produced by any compound having at least 2 peptide linkages in its molecule, e.g. proteins, peptides (except dipeptides) and biuret (formed ...
Full Article
Full Article

... cells and as a source of highly toxic oxidants used for microbicidal killing when produced in high concentrations by inducible NOS in macrophages [4]. ROS, as signalling molecules, regulate the expression of genes whose products serve important functions in the immune response, proliferation control ...
Wk12 Acid base_lec
Wk12 Acid base_lec

... • Are acids that do not leave solution • Once produced they remain in body fluids • Until eliminated by kidneys ...
Comparison of Amino Acid Sequences of Halloween Genes in
Comparison of Amino Acid Sequences of Halloween Genes in

ch24a_wcr
ch24a_wcr

... Institute Of Medicine (USA), 2002/2005, for adults. Recommended daily protein intake per kg is higher for children & pregnant & lactating women. © 2013 Pearson Education, Inc. ...
Tubulin folding is altered by mutations in a putative GTP binding motif
Tubulin folding is altered by mutations in a putative GTP binding motif

Engineering carbonic anhydrase for highly selective ester hydrolysis Gunnar Höst
Engineering carbonic anhydrase for highly selective ester hydrolysis Gunnar Höst

... which has four carbon atoms in the acyl chain, with an efficiency that is increased by a factor of 3000 compared to HCAII. Further, transition state analogues (TSAs) were docked to HCAII and mutant variants, and the results were correlated to the results from kinetic measurements. This indicated tha ...
Heterologous products from the yeast Kluyveromyces lactis
Heterologous products from the yeast Kluyveromyces lactis

... expression. This leads, in turn, to a progressive copy number decrease. The overall situation is thus a fluctuation of plasmid copy number around a physiological average value. In K. lactis, a host-vector system has been constructed in order to obtain a regulated system for copy number increase of e ...
Trafficking of phosphatidylinositol by phosphatidylinositol transfer
Trafficking of phosphatidylinositol by phosphatidylinositol transfer

... proteins that were able to bind and transport phospholipids, in particular PtdIns and PtdCho (phosphatidylcholine). The first mammalian PITP was purified from bovine brain [1] and its sequence was determined in 1989 [2]. It encoded a protein of 271 amino acids, which showed no sequence similarity wi ...
Chapter 2b
Chapter 2b

... Two or more polypeptide chains, each with its own tertiary structure, combine to form a functional protein. ...
Glycosaminoglycans and Ocular Structures
Glycosaminoglycans and Ocular Structures

... ~1 year of age. There is psychomotor retardation. Since the GAGs pile up in the joints and internal organs, there is a distorted facial appearance, deformed and stiff joints with an enlarged liver and spleen. In the eyes, the cornea becomes cloudy and the optic nerve degenerates. Also glaucoma may o ...
Fatty Acid Catabolism - Chemistry Courses: About
Fatty Acid Catabolism - Chemistry Courses: About

... Ketone Bodies • Water soluble form of lipids • Less potential energy than FA • Main energy source of brain in starvation • Also used in muscle and intestine ...
sheet#30
sheet#30

... Transport of ammonia to the liver: the amino group is removed in the form of ammonia which is toxic molecule, so it must not be let free in our system. It is very toxic to brain. The level of ammonia in the blood must be kept very low. Ammonia is produced by various tissues; it is converted to urea ...
From Sequence to Function
From Sequence to Function

Fatty Acid Oxidation
Fatty Acid Oxidation

... pathological states. Following this lecture students should understand that ...
Document
Document

... at one end – anticodon site for the hybridization with the mRNA template at the other end – attachment site for the amino acid that corresponds to the mRNA codon transcribed in the cytoplasm by RNA polymerase III – it folds into its ...
Triacylglycerol Metabolism Gone Bad: A major cause of disease
Triacylglycerol Metabolism Gone Bad: A major cause of disease

... Palmitoyl-CoA + 7FAD + 7NAD + 7CoA + 7H2O 8Acetyl-CoA + 7FADH2 + 7NADH + 7 H + ...
Spinalin, a new glycine- and histidine
Spinalin, a new glycine- and histidine

... which punches a hole in the prey’s integument. Nematocyst discharge is driven by the very high osmotic pressure (15 MPa; Weber, 1989) in the resting capsule, which results from the high concentration of poly-γ-glutamic acid and the corresponding cations in the matrix (Weber, 1990, 1991). The high os ...
Yeast ING Protein Yeast Protein Human Ortholog Description of
Yeast ING Protein Yeast Protein Human Ortholog Description of

... Description of Yeast gene function** Mitochondrial intermediate peptidase, cleaves N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Mitochondrial DNA-binding protein inv ...
Inborn Errors of Metabolism A Hospitalist`s Approach
Inborn Errors of Metabolism A Hospitalist`s Approach

... Modified from Clarke, 2002, Cambridge University Press, used with permission ...
Glycogen Metabolism and Gluconeogenesis
Glycogen Metabolism and Gluconeogenesis

Understanding a Millennium of Hemoglobin Evolution: Correlating
Understanding a Millennium of Hemoglobin Evolution: Correlating

... gene for Hb is ancient. It has been used to date the separation of the vertebrates and invertebrates some 1000 million (M) years ago. The emergence of a vertebrate Hb molecule with separate α- and β- chains occurred 500 – 600 M years later. Most studies of globin evolution to date have been by the c ...
Structural characterization of L
Structural characterization of L

... c-fragment (Tyr390). We infer that the larger a-fragment is an intermediate formed during protein maturation. An MS analysis of the c-fragment showed a sharp peak with a molecular mass of 10 570 Da (Fig. 1B), thereby enabling identification of the position of the C-terminal end of this fragment (Ala4 ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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