Aqua-BioSci. Monogr. (ABSM), Vol. 1, No. 2, pp. 1–57 (2008)
... The current methods of isolated hepatocytes are based on the procedure of Berry and Friend (1969). Berry (1976) has pointed out that any successful method for liver cell separation must involve three critical steps, namely exposure of the liver to a calcium-free medium, digestion with collagenase, a ...
... The current methods of isolated hepatocytes are based on the procedure of Berry and Friend (1969). Berry (1976) has pointed out that any successful method for liver cell separation must involve three critical steps, namely exposure of the liver to a calcium-free medium, digestion with collagenase, a ...
Seifert et al Fgf8 Dev2009
... This comparison of Fgf8 expression and cell lineage shows that Fgf8 is activated in the cloacal endoderm that makes contact with the ectoderm, and it is therefore possible that Fgf8 expression is a response to an inductive signal from the adjacent ectoderm. As the embryonic cloaca becomes subdivided ...
... This comparison of Fgf8 expression and cell lineage shows that Fgf8 is activated in the cloacal endoderm that makes contact with the ectoderm, and it is therefore possible that Fgf8 expression is a response to an inductive signal from the adjacent ectoderm. As the embryonic cloaca becomes subdivided ...
89 calcium handling by the mammalian kidney
... by feeding Ca2+ to the basolateral extrusion mechanisms (Feher et al. 1992). In this scheme, the diffusion of Ca2+ through the cytosol is the rate-limiting step in transcellular Ca2+ movement. In the basolateral membrane, two Ca2+ transporters, Ca2+-ATPase and a Na+/Ca2+ exchange mechanism, have bee ...
... by feeding Ca2+ to the basolateral extrusion mechanisms (Feher et al. 1992). In this scheme, the diffusion of Ca2+ through the cytosol is the rate-limiting step in transcellular Ca2+ movement. In the basolateral membrane, two Ca2+ transporters, Ca2+-ATPase and a Na+/Ca2+ exchange mechanism, have bee ...
Paramecium - tekkieoldteacher
... µm in length, depending on species. Simple cilia cover the body which allow the cell to move with a synchronous motion. There is also a deep oral groove containing inconspicuous compound oral cilia (as found in other peniculids) that is used to draw food inside. They generally feed upon bacteria and ...
... µm in length, depending on species. Simple cilia cover the body which allow the cell to move with a synchronous motion. There is also a deep oral groove containing inconspicuous compound oral cilia (as found in other peniculids) that is used to draw food inside. They generally feed upon bacteria and ...
Vitreous and Developmental Vitreoretinopathies
... resulting in surgical complications such as retinal breaks. The interface between vitreous and retina consists of a complex formed by the posterior vitreous cortex and the internal limiting lamina (ILL), which includes the basal lamina of Müller cells (62,63) (Fig. 3.6) (see also Chapter 4). The ILL ...
... resulting in surgical complications such as retinal breaks. The interface between vitreous and retina consists of a complex formed by the posterior vitreous cortex and the internal limiting lamina (ILL), which includes the basal lamina of Müller cells (62,63) (Fig. 3.6) (see also Chapter 4). The ILL ...
Involvement of extracellular calcium influx in the self
... 1999). Inositol trisphosphate (Ins(1,4,5)P3)-induced Ca2+ release has also been shown to play a role in the regulation of pollen tube growth (Franklin-Tong et al., 1996; MalhoÂ, 1998). Large, transient increases in [Ca2+]i have been observed in the `shank' of incompatible pollen tubes (Franklin-Tong ...
... 1999). Inositol trisphosphate (Ins(1,4,5)P3)-induced Ca2+ release has also been shown to play a role in the regulation of pollen tube growth (Franklin-Tong et al., 1996; MalhoÂ, 1998). Large, transient increases in [Ca2+]i have been observed in the `shank' of incompatible pollen tubes (Franklin-Tong ...
PDF - Blood Journal
... The platelet ␣IIb3 complex is a member of the integrin family of receptors, each of which is composed of an ␣ and a  subunit derived from separate genes. ␣IIb3 is important in platelet function, and both qualitative and quantitative disorders of ␣IIb3 result in the bleeding disorder Glanzmann th ...
... The platelet ␣IIb3 complex is a member of the integrin family of receptors, each of which is composed of an ␣ and a  subunit derived from separate genes. ␣IIb3 is important in platelet function, and both qualitative and quantitative disorders of ␣IIb3 result in the bleeding disorder Glanzmann th ...
VEGFR2 but not VEGFR3 governs integrity and remodeling of
... spanned by fenestral diaphragms, which is a critical component in the vasculatures of endocrine organs for proper transport of low-molecular weight hydrophilic molecules (Esser et al, 1998; Risau, 1998; Kamba et al, 2006). The propinquity of the fenestrated endothelium to thyroid follicular epitheli ...
... spanned by fenestral diaphragms, which is a critical component in the vasculatures of endocrine organs for proper transport of low-molecular weight hydrophilic molecules (Esser et al, 1998; Risau, 1998; Kamba et al, 2006). The propinquity of the fenestrated endothelium to thyroid follicular epitheli ...
Diacylglycerol kinase zeta in hypothalamus interacts with long form leptin receptor. Relation to dietary fat and body weight regulation
... other specific subcellular organelles (2– 6). Protein kinase C and receptor tyrosine kinase regulate both the enzymatic activity and subcellular location of the DGKs (4, 7–10). This report focuses on DGK, which belongs to a subgroup of DGKs that has unknown physiological function (10 –14). DGK is ...
... other specific subcellular organelles (2– 6). Protein kinase C and receptor tyrosine kinase regulate both the enzymatic activity and subcellular location of the DGKs (4, 7–10). This report focuses on DGK, which belongs to a subgroup of DGKs that has unknown physiological function (10 –14). DGK is ...
Increased Association Between Rough Endoplasmic Reticulum
... human form of tau P301L, true NFTs have been observed and correlated with neurodegeneration (19). This mutation is located in the second repeat of tau microtubule-binding domain and reduces its binding to microtubules (20). In JNPL3 mice, hyperphosphorylated tau accumulates in the somatodendritic co ...
... human form of tau P301L, true NFTs have been observed and correlated with neurodegeneration (19). This mutation is located in the second repeat of tau microtubule-binding domain and reduces its binding to microtubules (20). In JNPL3 mice, hyperphosphorylated tau accumulates in the somatodendritic co ...
Maternal control of the zebrafish organizer
... and F2 males of family no. 33. In 7 of 33 families, we were able to recover F2 females that transmitted the ichabod phenotype to their progeny (F3 embryos). Ratios of ichabod-transmitting females in four of the families generated from crosses of F1 siblings are presented. The number of F2 females ob ...
... and F2 males of family no. 33. In 7 of 33 families, we were able to recover F2 females that transmitted the ichabod phenotype to their progeny (F3 embryos). Ratios of ichabod-transmitting females in four of the families generated from crosses of F1 siblings are presented. The number of F2 females ob ...
... are expressed significantly less on alveolar macrophages than on monocytes (PNA, UEA-1, BSL 1, SIA) [51]. Many other receptors are also present on alveolar macrophages (table 1). There are receptors for macrophageactivating cytokines, such as interleukin-1 (IL-1), and TNF, which in addition to being ...
Bovine pneumonic pasteurellosis, more commonly known as
... which facilitates proliferation and survival of bacteria within the lung. Cytolysis of these cells also results in the release of their proteolytic enzymes and pro-inflammatory substances, which cause structural degradation of the alveolar epitheilial linings. Therefore, Lkt-induced lysis and degran ...
... which facilitates proliferation and survival of bacteria within the lung. Cytolysis of these cells also results in the release of their proteolytic enzymes and pro-inflammatory substances, which cause structural degradation of the alveolar epitheilial linings. Therefore, Lkt-induced lysis and degran ...
The Histone Variant H2A.W Defines Heterochromatin and Promotes
... C-terminal conserved motifs (Figure S1A available online) (Talbert et al., 2012). We obtained antibodies specific and representative of each H2A variant class (Figure S1B). We performed genomewide profiling by chromatin immunoprecipitation for H2A variant-bound DNA followed by deep sequencing (ChIP- ...
... C-terminal conserved motifs (Figure S1A available online) (Talbert et al., 2012). We obtained antibodies specific and representative of each H2A variant class (Figure S1B). We performed genomewide profiling by chromatin immunoprecipitation for H2A variant-bound DNA followed by deep sequencing (ChIP- ...
Regional requirements for Dishevelled signaling during Xenopus
... spot noise also implemented by the FlowJ plugin. In these images color is used to code the direction of flow. All images depict dorsal to the right. Statistics To evaluate blastopore phenotypes within a day of experiments, blastopore areas from different conditions were statistically evaluated with ...
... spot noise also implemented by the FlowJ plugin. In these images color is used to code the direction of flow. All images depict dorsal to the right. Statistics To evaluate blastopore phenotypes within a day of experiments, blastopore areas from different conditions were statistically evaluated with ...
Arabidopsis Formin3 Directs the Formation of Actin
... referred to as group I and group II. Group I formins are characterized by the presence of a signal peptide and a transmembrane domain at its N terminus, which are presumably involved in their targeting to the plasma membrane (PM). No conserved domains were identified beside FH1 and FH2 in group II f ...
... referred to as group I and group II. Group I formins are characterized by the presence of a signal peptide and a transmembrane domain at its N terminus, which are presumably involved in their targeting to the plasma membrane (PM). No conserved domains were identified beside FH1 and FH2 in group II f ...
EAP actuators aid the quest for the "Holy Braille" of tactile displays
... energize enough actuators to obtain reasonably short response or setup times. They also have problems associated with overheating due to accumulated heat dissipation of large numbers of closely packed actuators. This heat build up severely limits the dot cycling times, as it can take a long time for ...
... energize enough actuators to obtain reasonably short response or setup times. They also have problems associated with overheating due to accumulated heat dissipation of large numbers of closely packed actuators. This heat build up severely limits the dot cycling times, as it can take a long time for ...
Development 128, 4439-4447 (2001)
... (Fig. 2F) or in biochemical assays (Fig. 2G). A third cleavage site within the inter-repeat region has been described in vitro for mouse Chordin in the presence of Tsg (Scott et al., 2001). We did not detect this cleavage in Xenopus, either in vivo (Fig. 2E) or in vitro (Fig. 2G), but we note that t ...
... (Fig. 2F) or in biochemical assays (Fig. 2G). A third cleavage site within the inter-repeat region has been described in vitro for mouse Chordin in the presence of Tsg (Scott et al., 2001). We did not detect this cleavage in Xenopus, either in vivo (Fig. 2E) or in vitro (Fig. 2G), but we note that t ...
Stability of a promiscuous plasmid in different hosts: no
... Broad-host-range (BHR) IncP-1 plasmids have the ability to transfer between and replicate in nearly all species of the Alpha-, Beta- and Gammaproteobacteria, but surprisingly few data are available on the stability of these plasmids in strains within their host range. Moreover, even though molecular ...
... Broad-host-range (BHR) IncP-1 plasmids have the ability to transfer between and replicate in nearly all species of the Alpha-, Beta- and Gammaproteobacteria, but surprisingly few data are available on the stability of these plasmids in strains within their host range. Moreover, even though molecular ...
The scaffolding protein Cnk Interacts with Alk to Promote Visceral
... bioRxiv preprint first posted online Jan. 12, 2017; doi: http://dx.doi.org/10.1101/099986. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
... bioRxiv preprint first posted online Jan. 12, 2017; doi: http://dx.doi.org/10.1101/099986. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
An intracellular replication niche for Vibrio cholerae in the amoeba
... through contaminated water. Predatory protists, such as amoebae, are major regulators of bacterial populations in such environments. Therefore, we investigated the interaction between V. cholerae and the amoeba Acanthamoeba castellanii at the single-cell level. We observed that V. cholerae can resis ...
... through contaminated water. Predatory protists, such as amoebae, are major regulators of bacterial populations in such environments. Therefore, we investigated the interaction between V. cholerae and the amoeba Acanthamoeba castellanii at the single-cell level. We observed that V. cholerae can resis ...
Classification of Enteric Progenitors in Zebrafish During Development
... • Composed of different subtypes of neurons and glial cells ...
... • Composed of different subtypes of neurons and glial cells ...