lec3
... The hairpin destabilizes the DNA:RNA hybrid leading to dissociation of the RNA from the DNA. 2. Rho dependent: Rho protein binds to a sequence in the RNA (rut site – not well characterized). Rho moves along the RNA in the 3’ direction until in eventually unwinds the DNA:RNA hybrid in the active site ...
... The hairpin destabilizes the DNA:RNA hybrid leading to dissociation of the RNA from the DNA. 2. Rho dependent: Rho protein binds to a sequence in the RNA (rut site – not well characterized). Rho moves along the RNA in the 3’ direction until in eventually unwinds the DNA:RNA hybrid in the active site ...
Bio Chap 2 Biomolecules
... – In heredity – DNA makes up genes – Direct production of proteins Examples: DNA and RNA ...
... – In heredity – DNA makes up genes – Direct production of proteins Examples: DNA and RNA ...
Chapter 16 The Molecular Basis of Inheritance
... The other parental strand (5’ 3’ into the fork), the lagging strand, is copied away from the fork. ...
... The other parental strand (5’ 3’ into the fork), the lagging strand, is copied away from the fork. ...
chapter 17 and 18 study guide
... Promoter? A specific nucleotide sequence in the DNA that binds RNA polymerase, positioning it to start transcribing RNA at the appropriate place Repressor? A protein that inhibits gene transcription; in prokaryotes repressors bind to the DNA in or near the promoter; in eukaryotes repressors can bind ...
... Promoter? A specific nucleotide sequence in the DNA that binds RNA polymerase, positioning it to start transcribing RNA at the appropriate place Repressor? A protein that inhibits gene transcription; in prokaryotes repressors bind to the DNA in or near the promoter; in eukaryotes repressors can bind ...
Name: page1 of 7 pages MOLECULAR BIOLOGY BIO372S January
... 11. Which of the following isotopes would be the most appropriate for the end-labeling of a DNA strand with a radioactive phosphate via polynucleotide kinase? A. α-32P B. 35S C. β -32P D. γ -32P E. 14C ...
... 11. Which of the following isotopes would be the most appropriate for the end-labeling of a DNA strand with a radioactive phosphate via polynucleotide kinase? A. α-32P B. 35S C. β -32P D. γ -32P E. 14C ...
DNA
... • You will need to look up the mRNA codon and amino acid that corresponds to it using a table or a wheel ...
... • You will need to look up the mRNA codon and amino acid that corresponds to it using a table or a wheel ...
Microbial diversity
... Sequence of 16 S rRNA gene often used to compare organisms 16 S rRNA gene amplified by PCR PCR product sequenced and sequence compared with that of known organism New development: comparative genomics ...
... Sequence of 16 S rRNA gene often used to compare organisms 16 S rRNA gene amplified by PCR PCR product sequenced and sequence compared with that of known organism New development: comparative genomics ...
Bell Activity
... changes color in the presence of an acid or base is an indicator. Bromthymol blue indicator ...
... changes color in the presence of an acid or base is an indicator. Bromthymol blue indicator ...
This examination paper consists of 4 pages
... Can only be closed by making a new clone library Can only be closed by sequencing all clones in a library a second time 11. An EST (Expressed Sequence Tag) Is a protein sequence Is an RNA sequence Is a DNA sequence Is used to delineate regulatory sequence elements Is used in genetic mapping Consists ...
... Can only be closed by making a new clone library Can only be closed by sequencing all clones in a library a second time 11. An EST (Expressed Sequence Tag) Is a protein sequence Is an RNA sequence Is a DNA sequence Is used to delineate regulatory sequence elements Is used in genetic mapping Consists ...
Biotechnology - Kinam Park Homepage
... The $325 billion-a-year pharmaceutical business is America’s toughest and one of its most profitable. It’s riskier and more rigorous at just about every stage than any other business, from the towering biological uncertainties inherent in its mission to treat disease; to the 30to-1 failure rate in b ...
... The $325 billion-a-year pharmaceutical business is America’s toughest and one of its most profitable. It’s riskier and more rigorous at just about every stage than any other business, from the towering biological uncertainties inherent in its mission to treat disease; to the 30to-1 failure rate in b ...
04Johnson
... information is encoded in the nucleic acid by different sequences of these nucleotides ...
... information is encoded in the nucleic acid by different sequences of these nucleotides ...
doc summer 2010 lecture 1 pg. 1-27
... MESSAGE: The DNA of a gene can be used as a probe to find similar segments in a mixture of DNA molecules or RNA molecules. An antibody can be used as a probe to find a specific protein in a mixture of proteins. 1.5 : model organisms Model organisms: small number of species whose genetic mechanisms a ...
... MESSAGE: The DNA of a gene can be used as a probe to find similar segments in a mixture of DNA molecules or RNA molecules. An antibody can be used as a probe to find a specific protein in a mixture of proteins. 1.5 : model organisms Model organisms: small number of species whose genetic mechanisms a ...
point mutation
... a codon chart, we could determine what kind of point mutation it is: missense, nonsense, or ...
... a codon chart, we could determine what kind of point mutation it is: missense, nonsense, or ...
16.2 Biotechnology Products
... Gene cloning is production of many identical copies of the same gene. 1. If the inserted gene is replicated and expressed, we can recover the cloned gene or protein product. 2. Cloned genes have many research purposes: determining the base sequence between normal and mutated genes, altering the phen ...
... Gene cloning is production of many identical copies of the same gene. 1. If the inserted gene is replicated and expressed, we can recover the cloned gene or protein product. 2. Cloned genes have many research purposes: determining the base sequence between normal and mutated genes, altering the phen ...
Review Guide Genetics
... Nonsense – when the mutation changes an amino acid sequence into a stop codon. The process will stop too soon and the protein won’t be finished. This missing part of the protein will cause it to not function properly. (ex: you accidently cut off the last three words of the sentence so you don’t know ...
... Nonsense – when the mutation changes an amino acid sequence into a stop codon. The process will stop too soon and the protein won’t be finished. This missing part of the protein will cause it to not function properly. (ex: you accidently cut off the last three words of the sentence so you don’t know ...
Genetics BIO.B.1.2.1 Describe how the process of DNA replication
... Nonsense – when the mutation changes an amino acid sequence into a stop codon. The process will stop too soon and the protein won’t be finished. This missing part of the protein will cause it to not function properly. (ex: you accidently cut off the last three words of the sentence so you don’t know ...
... Nonsense – when the mutation changes an amino acid sequence into a stop codon. The process will stop too soon and the protein won’t be finished. This missing part of the protein will cause it to not function properly. (ex: you accidently cut off the last three words of the sentence so you don’t know ...
BioBoot Camp Genetics
... heterozygous genotype. When a dominant gene allele is present it will hide/mask the expression of other alleles and the organism will have the dominant characteristic. Recessive – trait where the phenotypic effect of the recessive allele is only expressed within a homozygous genotype. There must be ...
... heterozygous genotype. When a dominant gene allele is present it will hide/mask the expression of other alleles and the organism will have the dominant characteristic. Recessive – trait where the phenotypic effect of the recessive allele is only expressed within a homozygous genotype. There must be ...
C16 DNA
... of DNA separate to form “bubbles”. In eukaryotes there are 100’s – 1000’s of origin sites along the giant DNA molecule of each chromosome. In bacteria, there is only 1 origin of replication. Replication fork – found at each end of a replication bubble, Yshaped region where new strands of DNA are elo ...
... of DNA separate to form “bubbles”. In eukaryotes there are 100’s – 1000’s of origin sites along the giant DNA molecule of each chromosome. In bacteria, there is only 1 origin of replication. Replication fork – found at each end of a replication bubble, Yshaped region where new strands of DNA are elo ...
document
... DNA in nucleus, proteins made in cytoplasm RNA present in large quantities RNA moves from nucleus to cytoplasm Information transfer DNA->RNA->protein 1961 Francis Crick: codons Determination of genetic codes for each amino acid ...
... DNA in nucleus, proteins made in cytoplasm RNA present in large quantities RNA moves from nucleus to cytoplasm Information transfer DNA->RNA->protein 1961 Francis Crick: codons Determination of genetic codes for each amino acid ...
Document
... Transpositional site-specific recombination Conservative site-specific recombinatinon ...
... Transpositional site-specific recombination Conservative site-specific recombinatinon ...
SMU-DDE-Assignments-Scheme of Evaluation PROGRAM msc
... positive supercoiling, and the bases are held more tightly together. If they are twisted in the opposite direction, this is negative supercoiling, and the bases come apart more easily. 5. Quadruplex structures: In humans, the telomeres are the lengths of single stranded sequences, enriched with ...
... positive supercoiling, and the bases are held more tightly together. If they are twisted in the opposite direction, this is negative supercoiling, and the bases come apart more easily. 5. Quadruplex structures: In humans, the telomeres are the lengths of single stranded sequences, enriched with ...
Answers - loreescience.ca
... comparison of VNTR DNA in the samples rather than the DNA found in the genes. Explain why you think this is so. The characteristics of VNTR microsatellites (the DNA of which is non-coding) differ widely between different individuals. On the other hand, because variation in base sequence often has su ...
... comparison of VNTR DNA in the samples rather than the DNA found in the genes. Explain why you think this is so. The characteristics of VNTR microsatellites (the DNA of which is non-coding) differ widely between different individuals. On the other hand, because variation in base sequence often has su ...
04/01
... by one enzyme contains restriction sites for the other enzyme. Fragments are aligned by size. Enzyme 1: 8 kb, 6 kb, 3 kb or 3 kb, 6 kb, 8 kb 6 kb, 8 kb, 3 kb or 3 kb, 8 kb, 6 kb 8 kb, 3 kb, 6 kb or 6 kb, 3 kb, 8 kb Enzyme 2: 10 kb, 7 kb or 7 kb, 10 kb Double Digest: 3 kb fragment is split into 2 kb ...
... by one enzyme contains restriction sites for the other enzyme. Fragments are aligned by size. Enzyme 1: 8 kb, 6 kb, 3 kb or 3 kb, 6 kb, 8 kb 6 kb, 8 kb, 3 kb or 3 kb, 8 kb, 6 kb 8 kb, 3 kb, 6 kb or 6 kb, 3 kb, 8 kb Enzyme 2: 10 kb, 7 kb or 7 kb, 10 kb Double Digest: 3 kb fragment is split into 2 kb ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.