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The amino acids
The amino acids

... Di-peptide Amino acids bind, to form a protein. Upon binding, two protons from the NH3 and one oxygen from the carboxyl join to form a water. So the peptide bond has at the one side a C=O and at the other side an N-H. Only the ends of the chain are NH3 or carboxylic. ...
Part II: How are antibiotics discovered
Part II: How are antibiotics discovered

... a) Explain how a search for resistant mutants can give information as to the antibiotic’s target and mechanism of action. b) By adding the antibiotic (myx) at different times in a transcription reaction, the authors showed that myx inhibits RNA polymerase binding to double stranded DNA. Explain. ...
Isolation of Genomic DNA
Isolation of Genomic DNA

... Genomic DNA isolation from Ashbya gossypii ...
Activity--Extracting DNA - Challenger Learning Center
Activity--Extracting DNA - Challenger Learning Center

... Allow approximately 50 minutes for the procedure, clean-up, and students’ completing the reflection questions. You may have the students complete them for homework if you run out of time, or you may save them for discussion. Reflection and Discussion 1. What is DNA? The major component of chromosome ...
You should be able to find the information necessary to answer
You should be able to find the information necessary to answer

... 18) How many amino acids are there? Draw the structure of a typical amino acids circling and labeling the amino group and the carboxylic group. Provide named examples of each of the following types of amino acids: Polar R Groups, NonPolar R Groups, negatively charged, positively charged. ...
Exam 2
Exam 2

... Proteins whose binding to DNA is not dependent on specific base sequences generally form ionic bonds between (+) charged amino acids and (-) charges on the phosphate backbone of the DNA B helix. ...
Answer Key - Department of Chemistry ::: CALTECH
Answer Key - Department of Chemistry ::: CALTECH

... and the 3′ end a terminal hydroxyl group, which bind adjacent DNA via phosphodiester bonds, forming a phospho-deoxyribose backbone. (2 points) The bases (DNA) include Cytosine, Guanine, Adenine, and Thymine (depicted below) (2 points, 0.5 pt for each). In RNA Uracil takes the place of Thymine. (0.5 ...
the code of translation
the code of translation

... 4. A peptide bond forms between the first two amino acids. 5. The first tRNA leaves, and the ribosome moves along the mRNA to the next codon. 6. The next tRNA brings in the next amino acid, and a peptide bond is formed between this amino acid and the growing amino acid chain. 7. The process continu ...
What is Germicidal UV?
What is Germicidal UV?

... damage to the nucleic acid of microorganisms by forming covalent bonds between certain adjacent bases in the DNA. The formation of these bonds prevents the DNA from being “unzipped” for replication, and the organism is unable to reproduce. In fact, when the organism tries to replicate, it dies. UV-C ...
PowerPoint - Garnet Valley School District
PowerPoint - Garnet Valley School District

... The sequence of amino acids in the chain will determine the protein’s shape and function!!! ...
DEPARTMENT OF MICROBIOLOGY University of Delhi South campus New Delhi-110021 PhD Course work
DEPARTMENT OF MICROBIOLOGY University of Delhi South campus New Delhi-110021 PhD Course work

... Passed in DRC held on 12 January, 2016 ...
File S4 (DOC) - cloudfront.net
File S4 (DOC) - cloudfront.net

Biomolecules
Biomolecules

... Amino acids are usually colourless, crystalline solids. These are water-soluble, high melting solids and behave like salts rather than simple amines or carboxylic acids. This behaviour is due to the presence of both acidic (carboxyl group) and basic (amino group) groups in the same molecule. In aqu ...
Molecular Testing and Clinical Diagnosis
Molecular Testing and Clinical Diagnosis

... • Determines if target is present & its distribution within cells • Requires tissue sections, probe and visualization system • If fluorescent tag used = fluorescent in situ hybridization (FISH ) ...
No Slide Title
No Slide Title

... ...while attempting to do anti-sense KO of gene expression in C. elegans, Guo and Kemphues, Cell 81, 611 (1995) observed that sense and antisense strands worked equally at reducing transcript, – in an anti-sense experiment, a gene is constructed so that it produces a complementary strand to an expre ...
CHAPTER 4: CELLULAR METABOLISM
CHAPTER 4: CELLULAR METABOLISM

... Each DNA strand is made up of a backbone of deoxyribose sugars alternating with phosphate groups. See Fig 4.17, page 116. Each deoxyribose sugar is linked to one of four nitrogen-containing bases: A,G,C, or T. Each DNA molecule consists of two parallel strands of nucleotides running in opposite dire ...
Document
Document

... How are the instructions for assembling amino acids into proteins encoded into DNA? 20 amino acids - only four nucleotide bases in DNA how many nucleotides correspond to an amino acid? the mRNA nucleotide sequence is “read” in groups of 3 nucleotides = “codons” each codon codes for 1 of the 20 amino ...
Human Genetics
Human Genetics

... nucleotides at the 3’ end. Nucleic acids are oriented and by convention sequences are always written 5’ to 3’. Thus, ATTGCA 6= ACGTTA. The addition of new nucleotides is catalyzed by a polymerase. Nucleotides can be removed by nucleases. ...
gene-expression-text
gene-expression-text

... Normal fly ...
幻灯片 1
幻灯片 1

... provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence.  Whereas enzymes synthesize DNA and RNA in a 5' to 3' direction, chemical oligonucleotide synthesis is carried out in the opposite, 3' to 5' direction.  Currently, the process is implemented as solid-p ...
Big Data Study - Open Medicine Foundation
Big Data Study - Open Medicine Foundation

How to read a codon table - Waukee Community School District Blogs
How to read a codon table - Waukee Community School District Blogs

Telomeres - OpenWetWare
Telomeres - OpenWetWare

... identity between Rpa3 and Ten1, and therefore we cannot conclude whether Ten1 contains an OB-fold domain or not. This may be a reflection of the fact that both proteins have diverged rapidly at the primary sequence level, as revealed by the alignments of Rpa3 and Ten1 sequences from fungal genomes. ...
How to read a codon table
How to read a codon table

... an amino acid that will eventually form a protein that is used within a cell. • Proteins are made up of hundreds of amino acids in a specific sequence. • When they get “out of order’ a mutation ...
Lab 4 Restriction Enzyme Digestions and Mapping
Lab 4 Restriction Enzyme Digestions and Mapping

... So, what are restriction enzymes? Restriction enzymes, or restriction endonucleases, are proteins that recognize and bind to specific DNA sequences and cut the DNA at or near the recognition site. Restriction enzymes were originally discovered through their ability to break down, or "restrict" forei ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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