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... # These centrifuges are run in batch to isolate small volumes of biological macromolecules or cells. ...
60Ch14DNAhistory2008..
60Ch14DNAhistory2008..

...  DNA composition: “Chargaff’s rules” varies from species to species  all 4 bases not in equal quantity  bases present in characteristic ratio ...
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to find the lecture notes for lecture 1 click here

... e.g. H20 + NaCl -in a solution – a solvent dissolves another substance called a solute -water is a versatile solvent because of its polar covalent bonds and its bent shape which allows it to interact with its neighbours very well -water + salt: the electronegative O of water attracts the +ve sodium ...
PPT presentation
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... • Amino acid pairs are classified into two types: identical and non-identical • Non-identical pairs are scored 0 and identical pairs given a positive score (usually 1) • The scoring scheme is generally considered less effective than schemes that weight non-identical pairs ...
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Molecular Biology 240386

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... Secondary Structure in Proteins Within a single protein, different regions of the polypeptide chain assume different conformations determined by the primary sequence of amino acids. α-Helix: formation is stabilized by H-bonds between amino nitrogens and carbonyl carbons of the peptide bonds every 4 ...
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Chapter 13( Sample questions)

... d. Restriction fragment length polymorphism (RFLP) e. The polymerase chain reaction (PCR) Biotechnology cannot be used to: a. Produce large quantities of particular human proteins b. Produce effective and safe vaccines c. Identify human fetuses with particular genetic diseases d. Alter food plants t ...
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... The sugar can be deoxyribose (as in DNA or_____________________) or ribose (as in RNA or ______________________) DNA and RNA are central to heredity and are made unique by the nitrogenous ____________ that is attached Nitogenous bases can be cytosine (C), thymine (T), uracil (U), adenine (A), or gua ...
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... which breaks down DNA, destroyed the transforming power. Together, these results showed that neither proteins nor RNA carried the "instructions" (or the genes) which allowed transformation. These results also indicated that DNA was the heredity material. The year was 1944. However, this finding was ...
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The Spurious Foundation of Genetic Engineering

... Alternative splicing can have an extraordinary impact on the gene/protein ratio. We now know that a single gene originally believed to encode a single protein that occurs in cells of the inner ear of chicks (and of humans) gives rise to 576 variant proteins, differing in their amino acid sequences. ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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