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Chapter 7 Notes: DNA Profiling
Chapter 7 Notes: DNA Profiling

... • Contains genetic code for the production of proteins – Cell can replicate its DNA and carry on all life functions using these proteins! • all DNA found in human cells makes up the human genome – Only .5% of DNA (15 million nucleotides) determines the difference between individual people! • can be ...
Understanding DNA / Chromatin / Chromosomes
Understanding DNA / Chromatin / Chromosomes

... Step 6) What do the terms diploid and haploid mean? What do the symbols n or 2n mean? Make sure you know this in class. You do not need to write anything into your packet unless you want to. For the next two questions, here is a reminder of how to draw DNA structures within a nucleus: Focus on the n ...
2013 Training Handout
2013 Training Handout

Polymerase chain reaction
Polymerase chain reaction

DNA LABELING, HYBRIDIZATION, AND DETECTION (Non
DNA LABELING, HYBRIDIZATION, AND DETECTION (Non

... The reaction then is dependent on the number of molecules attached to the blot (and how available they are for pairing) and the concentration of the probe DNA molecules in solution. A third very important parameter is the temperature at which hybridization is camed out. Each sequence has a particula ...
page 18 - National Nanotechnology Infrastructure Network
page 18 - National Nanotechnology Infrastructure Network

... lengths, we designed the chip for continuous liquid flow, so that we could introduce fresh chemicals and flush out residual chemicals. We matched the dimensions of the chip to that of the capillary tube to eliminate dead volume areas where there could be a residual chemical build-up. By doing this, we ...
PEPTIDE BONDS AND POLYPEPTIDES OLIGOPEPTIDE
PEPTIDE BONDS AND POLYPEPTIDES OLIGOPEPTIDE

... polypeptides (and proteins) have a front end (amino terminus or N-terminus) and a back end (carboxyl terminus or C-terminus) •most proteins contain 50-2000 amino acids •mean molecular weight of an amino acid is 110 (see Problem #1) so MW of proteins could be 5500 to220,000 (ball park numbers) Polype ...
Lipids, Proteins, Nucleic Acids
Lipids, Proteins, Nucleic Acids

... Induced fit: an adjustment by the enzyme to fully fit to the substrate ...
Biochemistry - Ursuline High School
Biochemistry - Ursuline High School

... • Resist pH shifts. • Cells and other biological solutions often contain buffers to prevent damage. ...
MTC15 - toddgreen
MTC15 - toddgreen

... Multiple carbohydrates join together to form disaccharides (two simple sugars), oligosaccharides (two to ten simple sugars) and polysaccharides (many sugars) Simple sugars have both linear and ring forms and join together via glycosidic links formed between two –OH groups with the elimination of a w ...
fiii Fli I`.,
fiii Fli I`.,

... Amino acids produced by digestion of dietary protein and during protein turnover in body cells become part of the body's amino acid pool. The amino acid pool is the total quantity of free amino acids present in tissue cells,plasma, and other bodyfluids. The amino acids of the amino acid pool are ava ...
genomic library
genomic library

... Restriction endonucleases • Restriction enzymes cut DNA into specific fragments • Restriction enzymes recognize specific base sequences in double-stranded DNA and cleave both strands of the duplex at specific places • Characteristics of restriction enzymes: 1. Cut DNA sequence-specifically 2. Bacte ...
Know Your Chromosomes - Indian Academy of Sciences
Know Your Chromosomes - Indian Academy of Sciences

... The total amount of DNA in 23 chromosomes is estimated to be three billion (3 x 109 ) base pairs. Base pair means a pair consisting of adenine and thymine (A-T) or guanine and cytosine (G-C) the nitrogen containing bases in the building blocks of DNA. Therefore the total amount of DNA in our cell is ...
Non-translational synthesis of poly-amino
Non-translational synthesis of poly-amino

RT-PCR - Faperta UGM
RT-PCR - Faperta UGM

... effects would be multiplied and become uncontrollable For this reason, the authorities have adopted a cautious, case by case, and step by step approach The specific concerns in terms of effect on the environment can be summarized as 3 questions 1. Will the process of genetic modification make the pl ...
Chromosomes - ISGROeducation
Chromosomes - ISGROeducation

... Spacer regions include DNA that does not encode a protein product, and may function in spacing genes apart so that enzymes or other molecules can interact easily with them. ...
A-level Biology B Question paper Unit 2 - Genes and Genetic
A-level Biology B Question paper Unit 2 - Genes and Genetic

... (b) The GFP gene can be used as a marker to identify bacteria which have been genetically engineered. Bacteria containing the GFP gene glow green under UV light. Suggest two advantages of using the GFP gene as a genetic marker rather than genes ...
First Semester Exam Review (Word Doc)
First Semester Exam Review (Word Doc)

...  Describe the functions of the three types of RNA  Understand the process of translation including the role of tRNA, where it occurs, and what is produced  Label a diagram illustrating the process of protein synthesis  Read a codon chart using the appropriate molecule, mRNA  Convert a given seq ...
Genetic Changes - Down the Rabbit Hole
Genetic Changes - Down the Rabbit Hole

... Significance of Mutations Most are neutral • Eye color • Birth marks • Some are harmful • Cystic Fibrosis • Down Syndrome • Some are beneficial • Sickle Cell Anemia to Malaria • Immunity to HIV ...
Biology Keystone Review.2016.Part 2
Biology Keystone Review.2016.Part 2

... i. the principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes 12. Some alleles are neither dominant nor recessive, and many traits are controlled by multiple alleles or multiple genes a. incomplete dominance (Red X White ...
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Document

... Directional mutation pressure in DNA – The rates of mutation between the four bases are usually not equal. This causes a mutational pressure that drives the base frequencies away from 25%. If no selection acts on the DNA, base frequencies will reach an equilibrium determined by mutation. The frequen ...
A ZEPTO MOLE DNA MICRO SENSOR  *
A ZEPTO MOLE DNA MICRO SENSOR *

... limits the post fabrication (chip bonding) choices if a closed sensor is to be developed. All of these issues, which comes from these two cumbersome steps, add complexities to the lab-on-chip design. To remove the immobilization and washing steps, a molecular beacon (MB) based RNA -DNA hybridization ...
Chapter 4 Background DNA Structure and Analysis
Chapter 4 Background DNA Structure and Analysis

... • Main focus on genes and their function ...
Laboratory Safety Bio 181 I. General practices: 1. First and foremost
Laboratory Safety Bio 181 I. General practices: 1. First and foremost

Lab #5a Mr. Green Genes-DNA Sequence
Lab #5a Mr. Green Genes-DNA Sequence

... The efficient analysis of biological data to reveal useful information has become one of the most daunting challenges facing biologists. In the twenty years since 1995, when the first complete genome sequence was submitted to the databases, technology improvements have dramatically decreased the cos ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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