Cells in culture.
... When a DNA probe is being used to find DNAs that are related, but not identical, in sequence, hybridization is performed at a lower temperature. This allows even imperfectly paired double helices to form. Only the lower-temperature hybridization conditions can be used to search for genes (C and E in ...
... When a DNA probe is being used to find DNAs that are related, but not identical, in sequence, hybridization is performed at a lower temperature. This allows even imperfectly paired double helices to form. Only the lower-temperature hybridization conditions can be used to search for genes (C and E in ...
RNA processing - Faculty Web Pages
... does this take place? •What happens to the RNA molecule if it does not undergo processing? •Purpose of each RNA processing step –What is a methyl guanine “cap”? –How is a polyA tail added? How does this tail contribute to the stability of the mRNA? ...
... does this take place? •What happens to the RNA molecule if it does not undergo processing? •Purpose of each RNA processing step –What is a methyl guanine “cap”? –How is a polyA tail added? How does this tail contribute to the stability of the mRNA? ...
Amino Acid Exporter: A Tool for the Next
... acids, into medium. The former two aspects, in particular metabolic changes, have been the targets for the development of hyper-producing strains, but the last step has never so far received attention, in part, due to a lack of knowledge about the amino acids efflux systems. Significant improvements ...
... acids, into medium. The former two aspects, in particular metabolic changes, have been the targets for the development of hyper-producing strains, but the last step has never so far received attention, in part, due to a lack of knowledge about the amino acids efflux systems. Significant improvements ...
Chelatococcus sambhunathii sp. nov., a moderately thermophilic
... microscopy (model FEI MORGAGNI 268D). The presence of flagella was determined by using cells that were placed onto a carbon-coated grid and negatively stained with 2.0 % phosphotungstic acid (Sharma et al., 1989). Cells were rod-shaped, 0.8–1.0 mm wide and 2.3–2.7 mm long, motile by means of a singl ...
... microscopy (model FEI MORGAGNI 268D). The presence of flagella was determined by using cells that were placed onto a carbon-coated grid and negatively stained with 2.0 % phosphotungstic acid (Sharma et al., 1989). Cells were rod-shaped, 0.8–1.0 mm wide and 2.3–2.7 mm long, motile by means of a singl ...
FEBS Letters
... Site directed mutagenesis was conducted on the pET-15b-IN plasmid, a generous gift from Dr. Robert Craigie, Laboratory of Molecular Biology, NIDDK, NIH, Bethesda, MD. The plasmid contains full length WT IN fused to a linker containing a 6-residue N-terminal histidine tag downstream from a T7 promote ...
... Site directed mutagenesis was conducted on the pET-15b-IN plasmid, a generous gift from Dr. Robert Craigie, Laboratory of Molecular Biology, NIDDK, NIH, Bethesda, MD. The plasmid contains full length WT IN fused to a linker containing a 6-residue N-terminal histidine tag downstream from a T7 promote ...
From Genetics to DNA
... general, a base linked to a sugar is called a nucleoside and a base linked to a sugar and one or more phosphate groups is called a nucleotide. If multiple nucleotides are linked together, as in DNA, this polymer is referred to as a polynucleotide. The backbone of the DNA strand is made from alternat ...
... general, a base linked to a sugar is called a nucleoside and a base linked to a sugar and one or more phosphate groups is called a nucleotide. If multiple nucleotides are linked together, as in DNA, this polymer is referred to as a polynucleotide. The backbone of the DNA strand is made from alternat ...
Supplementary Notes
... distinct types of leaving groups: cyanomethyl esters (CME), thioesters, and adenosine monophosphate. This suggests that the leaving group is not involved in recognition of the substrate for Fx. Moreover, Fx accepts aminoacyl-donors with not only amino groups (including ammonium as well as N-acylat ...
... distinct types of leaving groups: cyanomethyl esters (CME), thioesters, and adenosine monophosphate. This suggests that the leaving group is not involved in recognition of the substrate for Fx. Moreover, Fx accepts aminoacyl-donors with not only amino groups (including ammonium as well as N-acylat ...
Free Response – due Friday, Oct 2 – typed – single
... monosaccharide, lipid, fatty acid, semi-permeable membrane, protein, amino acid, nucleic acid, nucleotide, genetic, energy. ...
... monosaccharide, lipid, fatty acid, semi-permeable membrane, protein, amino acid, nucleic acid, nucleotide, genetic, energy. ...
Who was Gregor Mendel and what did he do?
... be all tall in the first cross and that a recessive factor was being expressed in the second cross. Principle of Dominance: When an organism is crossed for a pair of contrasting traits ONLY the dominant trait can be seen in the hybrid. The recessive factor was hidden. ...
... be all tall in the first cross and that a recessive factor was being expressed in the second cross. Principle of Dominance: When an organism is crossed for a pair of contrasting traits ONLY the dominant trait can be seen in the hybrid. The recessive factor was hidden. ...
E. coli - Department of Chemistry
... Glycerol by yeast fermentation of sugars, (and by hydrolysis of fats and oils) Glutamic acid by fermentation of glucose or xylose with B. subtilis or genetically ...
... Glycerol by yeast fermentation of sugars, (and by hydrolysis of fats and oils) Glutamic acid by fermentation of glucose or xylose with B. subtilis or genetically ...
Lecture 7 - Columbus Labs
... to therapeutics to identify genes involved in sensitivity and resistance ...
... to therapeutics to identify genes involved in sensitivity and resistance ...
DNA sequence of the control region of phage D108: the N
... The Mu leftward promoter at position 1098 to 1074 had a lower score but has been Identified as one of the promoters for the repressor gene (16). The -10 region and -35 region are boxed and nucleotides in these regions that form part of the consensus sequence are shown in large letters. ...
... The Mu leftward promoter at position 1098 to 1074 had a lower score but has been Identified as one of the promoters for the repressor gene (16). The -10 region and -35 region are boxed and nucleotides in these regions that form part of the consensus sequence are shown in large letters. ...
Virtual Molecular Lab: Is an Endangered Species Being Traded
... PCR machine. Close the lid and click the green arrow to start the machine. This process, called polymerase chain reaction (PCR), makes multiple copies of the particular gene you are targeting—in this case, ATP6. (For help running the PCR, watch this video.) PCR takes a while, so click on the arrows ...
... PCR machine. Close the lid and click the green arrow to start the machine. This process, called polymerase chain reaction (PCR), makes multiple copies of the particular gene you are targeting—in this case, ATP6. (For help running the PCR, watch this video.) PCR takes a while, so click on the arrows ...
05_lecture_presentation
... Storage proteins Function: Storage of amino acids Examples: Casein, the protein of milk, is the major source of amino acids for baby mammals. Plants have storage proteins in their seeds. Ovalbumin is the protein of egg white, used as an amino acid source for the developing embryo. ...
... Storage proteins Function: Storage of amino acids Examples: Casein, the protein of milk, is the major source of amino acids for baby mammals. Plants have storage proteins in their seeds. Ovalbumin is the protein of egg white, used as an amino acid source for the developing embryo. ...
Enzyme Mechanisms - Illinois Institute of Technology
... cloned inserts in expression vectors, viz. make RNA or even protein from it RNA: stick an efficient promoter next to the cloning site; vector DNA transcribed in vitro using SP6 RNA polymerase This can be used as a way of making radiolabeled RNA ...
... cloned inserts in expression vectors, viz. make RNA or even protein from it RNA: stick an efficient promoter next to the cloning site; vector DNA transcribed in vitro using SP6 RNA polymerase This can be used as a way of making radiolabeled RNA ...
Proteins and Enzymes
... think breaks down lactose? Remember amylase breaks down amylose 4. Individuals who are lactose intolerant cannot digest milk properly. Based on the information from this lab activity, what can you conclude about their body that would make it impossible for them to break down lactose products? ...
... think breaks down lactose? Remember amylase breaks down amylose 4. Individuals who are lactose intolerant cannot digest milk properly. Based on the information from this lab activity, what can you conclude about their body that would make it impossible for them to break down lactose products? ...
Origin of amino acid homochirality: Relationship with the RNA world
... I will focus on the origin of the homochirality of amino acids based on the aminoacylation of primitive tRNA. It is definitely the first step where RNA encounters amino acids not only in the current biological system and but also possibly in the prebiotic stage. I will present recent experimental ev ...
... I will focus on the origin of the homochirality of amino acids based on the aminoacylation of primitive tRNA. It is definitely the first step where RNA encounters amino acids not only in the current biological system and but also possibly in the prebiotic stage. I will present recent experimental ev ...
Prodigiosin Production in E. Coli
... have been over diluted (we had our samples suspended in 1ml of solution, when Dr. Schwekendiek noted 100µl was the usual dilution) - To rectify this, we concentrated our DNA in a Speed Vacuum Concentrator overnight - After running our samples through the Speed Vacuum Concentrator, we ran them throug ...
... have been over diluted (we had our samples suspended in 1ml of solution, when Dr. Schwekendiek noted 100µl was the usual dilution) - To rectify this, we concentrated our DNA in a Speed Vacuum Concentrator overnight - After running our samples through the Speed Vacuum Concentrator, we ran them throug ...
Document
... Internal and External Forces Acidic or basic amino acids with charged side chains congregate on the exterior of the protein where they can be solvated by water Amino acids with neutral, nonpolar side chains congregate on the hydrocarbon-like interior of a protein molecule Also important for stabili ...
... Internal and External Forces Acidic or basic amino acids with charged side chains congregate on the exterior of the protein where they can be solvated by water Amino acids with neutral, nonpolar side chains congregate on the hydrocarbon-like interior of a protein molecule Also important for stabili ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.