Supplementary Table 1: WormBase IDs and given
... yk1496c06); a singe poly(A) signal inferred from the ESTs; tat-2: amplified near full-length using GSP_tat-2_1 and SL1 (SL2 did not produce a product), sequenced 3 clones fully, 10 partially at both ends; 1 EST sequenced (yk209d5); a single poly(A) signal inferred from the EST data at WormBase; tat- ...
... yk1496c06); a singe poly(A) signal inferred from the ESTs; tat-2: amplified near full-length using GSP_tat-2_1 and SL1 (SL2 did not produce a product), sequenced 3 clones fully, 10 partially at both ends; 1 EST sequenced (yk209d5); a single poly(A) signal inferred from the EST data at WormBase; tat- ...
The Bacterial Heterotrimeric Amidotransferase GatCAB
... Gln-tRNAGln (when n=2) (Adapted from [8]). ...
... Gln-tRNAGln (when n=2) (Adapted from [8]). ...
Prying Into Prions - Colorado Parks and Wildlife
... activities found in most high school biology textbooks that address the chemistry of life, DNA and the genetic code, and protein synthesis. It is the third module developed by the DOW to address the specific learning objectives of high school students. Materials are inquiry based, develop critical t ...
... activities found in most high school biology textbooks that address the chemistry of life, DNA and the genetic code, and protein synthesis. It is the third module developed by the DOW to address the specific learning objectives of high school students. Materials are inquiry based, develop critical t ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... lower than the example shown above? What would be the melting temperature of the 18 base pair sequence if it was made up of only G-C pairs? Only A-T pairs? ...
... lower than the example shown above? What would be the melting temperature of the 18 base pair sequence if it was made up of only G-C pairs? Only A-T pairs? ...
Method To Develop High Oleic Acid Soybeans Using Conventional
... the number of carbon atoms in the fatty acid chain and the number of double bonds therein, Table 1. As used herein, the ...
... the number of carbon atoms in the fatty acid chain and the number of double bonds therein, Table 1. As used herein, the ...
genetic code: a new understanding of codon
... glycine type (with only G amino acid), proline type (only P), valine type (V & I) and alanine type (the rest of 16 AAs, each amino acid with a H-C-H “screen” between the “head” and “body”, i.e. side chain; the exception is threonine with an H-C-CH3 “screen”). The appearance of glycine corresponds to ...
... glycine type (with only G amino acid), proline type (only P), valine type (V & I) and alanine type (the rest of 16 AAs, each amino acid with a H-C-H “screen” between the “head” and “body”, i.e. side chain; the exception is threonine with an H-C-CH3 “screen”). The appearance of glycine corresponds to ...
Phospholipid Ester-linked Fatty Acid Biomarkers of
... (40 mM, gradually added). Desulfobacter postgatei, Desulfobacter strain AcBa and Desulfotomaculum acetoxidans were grown in duplicate cultures on acetate, one of each pair containing, in addition, the following VFA (final concentrations in mM) :propionate (2), isobutyrate (I), isovalerate (I), 2-met ...
... (40 mM, gradually added). Desulfobacter postgatei, Desulfobacter strain AcBa and Desulfotomaculum acetoxidans were grown in duplicate cultures on acetate, one of each pair containing, in addition, the following VFA (final concentrations in mM) :propionate (2), isobutyrate (I), isovalerate (I), 2-met ...
immobilised metal affinity chromatography (imac) beads for
... by coating with a hydrophilic and functional natural polymer. According to Wu et al, silica beads cross-linked with chitosan indicate convenient matches for metal ion adsorption [5]. Chitosan (CTS) is widely used for the hydrophilic modification due to its biocompatibility, biodegradability, chemica ...
... by coating with a hydrophilic and functional natural polymer. According to Wu et al, silica beads cross-linked with chitosan indicate convenient matches for metal ion adsorption [5]. Chitosan (CTS) is widely used for the hydrophilic modification due to its biocompatibility, biodegradability, chemica ...
Nonenzymatic, Self-Elimination Degradation
... glutathione into GSSG using oxidizing agents and formation of pyroglutamic acid from GSH at high temperature was suggested in previous studies [11]. In addition to the well-known enzymatic reactions of GSH, there are previously unreported nonenzymatic side reactions occurring, which include oxidatio ...
... glutathione into GSSG using oxidizing agents and formation of pyroglutamic acid from GSH at high temperature was suggested in previous studies [11]. In addition to the well-known enzymatic reactions of GSH, there are previously unreported nonenzymatic side reactions occurring, which include oxidatio ...
Further characterization of the lipoic acid enantiomers
... PI3K/Akt via increased phosphorylation of the insulin receptor and IRS-1. (Kiemer & Diesel 2008, Diesel et al 2007, Yaworsky et al ...
... PI3K/Akt via increased phosphorylation of the insulin receptor and IRS-1. (Kiemer & Diesel 2008, Diesel et al 2007, Yaworsky et al ...
Metabolic Acidosis
... Type B Lactic Acidosis • Low rate of acid production, otherwise acidosis would have killed the patient • Normal ECF volume rules out DKA and AKA • No history of GI problem rules out D-lactic acidosis • L-Lactic acid level was higher than 30 mmol/L and the patient was taking metformin for the treatm ...
... Type B Lactic Acidosis • Low rate of acid production, otherwise acidosis would have killed the patient • Normal ECF volume rules out DKA and AKA • No history of GI problem rules out D-lactic acidosis • L-Lactic acid level was higher than 30 mmol/L and the patient was taking metformin for the treatm ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... locations, the requirement of a TTTV PAM may limit the availability of suitable target sites, reducing the practical utility of Cpf1. To address this limitation, we aimed to engineer variants of Cpf1 that can recognize alternative PAM sequences in order to increase its targeting range. Previous work ...
... locations, the requirement of a TTTV PAM may limit the availability of suitable target sites, reducing the practical utility of Cpf1. To address this limitation, we aimed to engineer variants of Cpf1 that can recognize alternative PAM sequences in order to increase its targeting range. Previous work ...
Functions of DNA methylation: islands, start sites, gene bodies and
... analyse bisulphite-treated DNA. Reduced representation bisulphite sequencing is an approach in which DNA is cleaved by methylation-sensitive restriction enzymes before bisulphite treatment. The most comprehensive coverage at single-base level is obtained by shotgun sequencing of bisulphite-treated D ...
... analyse bisulphite-treated DNA. Reduced representation bisulphite sequencing is an approach in which DNA is cleaved by methylation-sensitive restriction enzymes before bisulphite treatment. The most comprehensive coverage at single-base level is obtained by shotgun sequencing of bisulphite-treated D ...
Point Mutation in Intron Sequence Causes Altered Carboxyl
... primer cycle sequencing kit (Amersham, Cleveland, OH). Cloning strategy. Each clone was first sequenced from an H/W rat. If any differences from the published SD rat receptor base order were detected (or if there were compression areas), the reciprocal strand of the DNA double helix was analyzed on ...
... primer cycle sequencing kit (Amersham, Cleveland, OH). Cloning strategy. Each clone was first sequenced from an H/W rat. If any differences from the published SD rat receptor base order were detected (or if there were compression areas), the reciprocal strand of the DNA double helix was analyzed on ...
Systems Biology Investigation to Discover Metabolic Biomarkers of
... liver injury in the clinical setting. ALT is an organ damage biomarker for hepatocyte injury, however, other factors can also influence its blood levels [3,4]. Therefore, ALT is not specific for liver injury [5-8]. Unfortunately, ALT and AST are often considered as liver functional biomarkers; these ...
... liver injury in the clinical setting. ALT is an organ damage biomarker for hepatocyte injury, however, other factors can also influence its blood levels [3,4]. Therefore, ALT is not specific for liver injury [5-8]. Unfortunately, ALT and AST are often considered as liver functional biomarkers; these ...
Sequence Heterogeneities Among 16s
... For phylogeny, rRNA sequences have become popular because they are universally present and have a conserved function (they are homologous in all organisms), because they are easy to sequence, and finally because they are constituted of an interspersion of highly conserved to very variable domains. R ...
... For phylogeny, rRNA sequences have become popular because they are universally present and have a conserved function (they are homologous in all organisms), because they are easy to sequence, and finally because they are constituted of an interspersion of highly conserved to very variable domains. R ...
USGENE - Chemical Abstracts Service
... nucleotide sequence data by similarity (homology). BLAST is provided in USGENE with the permission of the National Center for Biotechnology Information (NCBI) of the National Library of Medicine (NLM). GETSIM is provided in USGENE by FIZ Karlsruhe GmbH, and is based upon the FASTA algorithm. To init ...
... nucleotide sequence data by similarity (homology). BLAST is provided in USGENE with the permission of the National Center for Biotechnology Information (NCBI) of the National Library of Medicine (NLM). GETSIM is provided in USGENE by FIZ Karlsruhe GmbH, and is based upon the FASTA algorithm. To init ...
Enteric Microbes, Water Sources and Water Treatment
... • Disinfection activity can be expressed as the product of disinfection concentration (C) and contact time (T) Assumes first order kinetics (Chick’s Law) such that disinfectant concentration and contact time have the same “weight” or contribution in disinfection activity and in contributiong to CT • ...
... • Disinfection activity can be expressed as the product of disinfection concentration (C) and contact time (T) Assumes first order kinetics (Chick’s Law) such that disinfectant concentration and contact time have the same “weight” or contribution in disinfection activity and in contributiong to CT • ...
CBSE XII BIO QS with key(2009-2015)
... the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual reproduction producing seeds without fertilization/ t ...
... the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual reproduction producing seeds without fertilization/ t ...
Enteric Microbes, Water Sources and Water Treatment
... • Disinfection activity can be expressed as the product of disinfection concentration (C) and contact time (T) Assumes first order kinetics (Chick’s Law) such that disinfectant concentration and contact time have the same “weight” or contribution in disinfection activity and in contributiong to CT • ...
... • Disinfection activity can be expressed as the product of disinfection concentration (C) and contact time (T) Assumes first order kinetics (Chick’s Law) such that disinfectant concentration and contact time have the same “weight” or contribution in disinfection activity and in contributiong to CT • ...
Permianibacter aggregans gen. nov., sp. nov., a bacterium - YIC-IR
... methanol/water (65 : 25 : 4, by vol.), followed by chloroform/acetic acid/methanol/water (40 : 7.5 : 6.2, by vol.). Ethanolic molybdophosphoric acid (5 %) was applied to detect the presence of all lipids. Ninhydrin (0.2 % in watersaturated butanol) was used for revealing the presence of phospholipid ...
... methanol/water (65 : 25 : 4, by vol.), followed by chloroform/acetic acid/methanol/water (40 : 7.5 : 6.2, by vol.). Ethanolic molybdophosphoric acid (5 %) was applied to detect the presence of all lipids. Ninhydrin (0.2 % in watersaturated butanol) was used for revealing the presence of phospholipid ...
"Electromagnetic Interactions Between Fluorescent Dipoles and Metal Nanoparticles"
... Figure 2.5 TEM image with size analysis for 6.6nm AuNPs showing a standard deviation of 0.57 nm. Scale bar in image represents 50nm ...............................................................................27 Figure 2.6 TEM image with size analysis for 16.5nm AuNPs showing a standard deviation ...
... Figure 2.5 TEM image with size analysis for 6.6nm AuNPs showing a standard deviation of 0.57 nm. Scale bar in image represents 50nm ...............................................................................27 Figure 2.6 TEM image with size analysis for 16.5nm AuNPs showing a standard deviation ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.