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gen-305-presentation-8-16
gen-305-presentation-8-16

The Effects of Plasmids of Genotype and Phenotype
The Effects of Plasmids of Genotype and Phenotype

... would ordinarily kill the host. For example, plasmids can encode proteins that can break down antibiotics that would otherwise prevent the bacterial cell from growing. One such protein is an enzyme called beta-lactamase which can break down penicillin and certain modified penicillins such as ampicil ...
ALE 11. Genetics of Viruses, Recombinant DNA Technology, Gene
ALE 11. Genetics of Viruses, Recombinant DNA Technology, Gene

... sample of human DNA (i.e. human cheek cells on a cotton swab) explain how PCR (polymerase chain reaction) could be used to make millions of copies of the CFTR gene. Your explanation should include the roles of each of the following: primers, taq DNA polymerase (a heat-stable DNA polymerase from a ba ...
Exam 4
Exam 4

MolecularModeling3
MolecularModeling3

SAY IT WITH DNA: PROTEIN SYNTHESIS WORKSHEET: Practice
SAY IT WITH DNA: PROTEIN SYNTHESIS WORKSHEET: Practice

... STEP 1: "Build" the mRNA molecule, matching the RNA nucleotides to the DNA nucleotides properly, letter by letter. (For purposes of simplicity, it will be assumed that this mRNA is bacterial; there are no introns to cut out!) STEP 2: Figure out the tRNA triplets (codons) that would fit the mRNA trip ...
Nadine Noelting
Nadine Noelting

... associated with my gene are found in this conserved domain. The family consists of nonheme, iron (II)- dependent enzymes, including phenylalanine hydroxylase, eukaryotic tyrosine hydroxylase, and eukaryotic tryptophan hydroxylase. Each enzyme contains an ion metal binding site. PAH is involved in ph ...
L.R. Huang, J.O. Tegenfeldt, J.J. Kraeft, J.C. Sturm, R.H. Austin, E.C. Cox, "Generation of large-area tunable uniform electric fields in microfluid arrays for rapid DNA separation," Tech. Dig. Int. Elect. Dev. Mtg., pp. 363-366 (2001).
L.R. Huang, J.O. Tegenfeldt, J.J. Kraeft, J.C. Sturm, R.H. Austin, E.C. Cox, "Generation of large-area tunable uniform electric fields in microfluid arrays for rapid DNA separation," Tech. Dig. Int. Elect. Dev. Mtg., pp. 363-366 (2001).

... molecules over three orders of magnitude faster than conventional methods. Introduction Most biologically important molecules, such as proteins and deoxyribonucleic acids (DNA), are electrically charged. A very effective means to manipulate these molecules on microscopic scale is using electric fiel ...
Chapter 21
Chapter 21

... Introduction In most living organisms, the pathways by which a compound is synthesized are usually different from the pathways by which it is degraded; two reasons are 1. Flexibility: If a normal biosynthetic pathway is blocked, the organism can often use the reverse of the degradation pathway for ...
BioInformatics Tools ppt
BioInformatics Tools ppt

... Set of scores is used for comparison of two proteins, with or without gaps. MACAW uses scores for aligning amino acids called PAM-250. The score of the block is the sum of the scores assigned to each of its columns. Score of the column is the sum of all pairwise similarity scores of the amino acids ...
Cyclooxygenase (depicted above) inhibited by Salicylic Acid
Cyclooxygenase (depicted above) inhibited by Salicylic Acid

... • Is recorded written history as far back as 1500 B.C. Egyptian Physician Papyrus 500 B.C. by the Greek doctor Hippocrates. • This remedy was also among the ancient Sumer, Lebanon, Assyria as well as the Cherokee and other Native Americans. • End of the 19th century was regularly prescribed as pain ...
Fredrik Lysholm Bioinformatic	methods	for	characterization	of viral	pathogens	in	metagenomic	samples Linköping studies in science and technology
Fredrik Lysholm Bioinformatic methods for characterization of viral pathogens in metagenomic samples Linköping studies in science and technology

... protein databases, while retaining the underlying 454 data representation. Furthermore, a simulator for 454 data was developed in order to evaluate these methods. This simulator is currently the fastest and most complete simulator of 454 data, which enables further development of algorithms and meth ...
Extraction of DNA from soil
Extraction of DNA from soil

... enrichment targets isolates for their ability to metabolize specific substrates or to degrade deleterious xenobiotic compounds. However, only bacterial isolates for which optimal cultivation criteria have been determined can be recovered by this approach. The emerging use of molecular biology in mic ...
投影片 1
投影片 1

... 18CO2 + 17H2O + 122ATP + CoA-SH one 18C fatty acid ...
Chapter 17 - cloudfront.net
Chapter 17 - cloudfront.net

... 7. Describe where transcription and translation occur in prokaryotes and in eukaryotes; explain why it is significant that in eukaryotes, transcription and translation are separated in space and ...
Recovery of DNA for Forensic Analysis from Lip Cosmetics*
Recovery of DNA for Forensic Analysis from Lip Cosmetics*

Cancer Prone Disease Section Nijmegen breakage syndrome Atlas of Genetics and Cytogenetics
Cancer Prone Disease Section Nijmegen breakage syndrome Atlas of Genetics and Cytogenetics

... High frequency and early development of lymphomas, more often involving B-cells, in contrast with those found in AT; other forms of cancer may also be at higher risk. ...
Cellular Respiration - Peoria Public Schools
Cellular Respiration - Peoria Public Schools

... • What happens? 1.NADH and FADH2 carry their electrons/H+ to inner mit. comp. and “drop” them off. 2.H+ passes through a H+ pump to outer mit. comp. (more H+ outside than inside) 3.H+ diffuses back into inner mit. comp. 4.As H+ diffuses the reaction of ADP + P  ATP occurs. 5.H+ combines with O2 to ...
Biology: Life on Earth
Biology: Life on Earth

... The lactose operon consists of a regulatory gene, a promoter, an operator, and three structural genes that code for enzymes Involved in lactose metabolism. The regulatory gene codes for a protein, called a repressor, which can bind to the operator site ...
What are proteins
What are proteins

... favorable than the one in which the hydrophobic from the water matrix. substances were separate. Thus this combined state will persist. ...
File
File

Roundup Ready Canola RT73 DNA Detection Method
Roundup Ready Canola RT73 DNA Detection Method

... all samples using the standard curve for RT73 PCR and that for FatA PCR, respectively, and are then used to calculate the relative content (%) of RT73 DNA to total canola DNA by the following formula: relative content (%) of RT73 DNA to total canola DNA = [(concentration of RT73 DNA) / (concentratio ...
Defective HIV-1 Proviruses Can Be Transcribed Upon Activation
Defective HIV-1 Proviruses Can Be Transcribed Upon Activation

... transcribed and translated upon activation, such as by antigen stimulation, by homeostatic proliferation and by latency reversing agents used in the shock-and-kill strategy for HIV-1 eradication. Transcription of defective proviruses may complicate the correct estimation of the efficacy of latency r ...
Ever since the days of Rene Descartes, the French philosopher
Ever since the days of Rene Descartes, the French philosopher

... acts as an insect vector to transfer the malarial parasite into human body. In the same way, a plasmid can be used as vector to deliver an alien piece of DNA into the host organism. The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which ac ...
Medical Biochemistry: Course content 2016/2017
Medical Biochemistry: Course content 2016/2017

... names, structural formulas for understanding, names of enzymes and coenzymes. ATPconsuming steps, ATP-producing steps = substrate level phosphorylations, redox reactions (aerobically and anaerobically, respectively). Energy balance for glycolysis. ATP-production under aerobic and anaerobic condition ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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