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BioFiles v5 n1 - Sigma
... Lectins are a historically recognized group of non-immunogenic proteins that have preferential binding for specific carbohydrate ligands. Lectin conjugates can be used to probe tissues in a technique similar to immunohistochemistry, using the carbohydrate-binding property of the lectin to attach to ...
... Lectins are a historically recognized group of non-immunogenic proteins that have preferential binding for specific carbohydrate ligands. Lectin conjugates can be used to probe tissues in a technique similar to immunohistochemistry, using the carbohydrate-binding property of the lectin to attach to ...
Cleavage, Deprotection and Isolation of Peptides after Fmoc Synthesis
... If cleavage is performed on a PAL or Rink amide resin with a cocktail other than Reagent R or B, the resin may turn from pink to red, depending on the amount of scavengers in the cleavage cocktail. This should not effect the peptide quality. ...
... If cleavage is performed on a PAL or Rink amide resin with a cocktail other than Reagent R or B, the resin may turn from pink to red, depending on the amount of scavengers in the cleavage cocktail. This should not effect the peptide quality. ...
Advantages of compound-specific stable isotope
... amino acid tracer. This overestimation could be the result of two processes: First, labelled amino acids are subject to microbial cleavage, producing labelled C- and N-fragments in the soil. Plant uptake of these C-skeletons would lead to an overestimation of intact uptake.10 (Fig. 1(B)). Second, pl ...
... amino acid tracer. This overestimation could be the result of two processes: First, labelled amino acids are subject to microbial cleavage, producing labelled C- and N-fragments in the soil. Plant uptake of these C-skeletons would lead to an overestimation of intact uptake.10 (Fig. 1(B)). Second, pl ...
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... Silica gel column chromatography Paper chromatography of silica gel chromatography fractions Thin layer chromatography of the trans-methyiglutaconate shunt intermediates Determination of alcohol dehydrogenase activity in the postmitochondrial supernatant Isolation and characterization of proteins an ...
... Silica gel column chromatography Paper chromatography of silica gel chromatography fractions Thin layer chromatography of the trans-methyiglutaconate shunt intermediates Determination of alcohol dehydrogenase activity in the postmitochondrial supernatant Isolation and characterization of proteins an ...
Extending the limits of paleodietary studies of humans with
... highest standard deviations in 13C. Serine and threonine also have high standard deviations, possibly because of analytical concerns. During hydrolysis of complex tissues, especially high carbohydrate materials like plants, the hydroxlyated amino acids can be destroyed with attendant isotopic alter ...
... highest standard deviations in 13C. Serine and threonine also have high standard deviations, possibly because of analytical concerns. During hydrolysis of complex tissues, especially high carbohydrate materials like plants, the hydroxlyated amino acids can be destroyed with attendant isotopic alter ...
OXIDATION OF FATTY ACIDS (LIPOLYSIS) Fatty acids stored in
... hydrolysis of triacylglycerol .This process is initiated by hormonesensitive lipase (HSL), which removes a fatty acid from carbon 1 and/or carbon 3 of the TAG. Epinephrine (as well as norepinephrine) and glucagon stimulate fatty acid release from triglycerides stored in adipocyte fat droplets, where ...
... hydrolysis of triacylglycerol .This process is initiated by hormonesensitive lipase (HSL), which removes a fatty acid from carbon 1 and/or carbon 3 of the TAG. Epinephrine (as well as norepinephrine) and glucagon stimulate fatty acid release from triglycerides stored in adipocyte fat droplets, where ...
A novel zinc-dependent D-serine dehydratase
... [12]. In S. cerevisiae cells, D-tyrosine was found to serve as a substrate of tyrosine tRNA synthase [13]. It is possible that the formation of D-aminoacyl tRNAs is one of the reasons for the toxicity of D-amino acids to S. cerevisiae cells. S. cerevisiae possesses several protective systems against ...
... [12]. In S. cerevisiae cells, D-tyrosine was found to serve as a substrate of tyrosine tRNA synthase [13]. It is possible that the formation of D-aminoacyl tRNAs is one of the reasons for the toxicity of D-amino acids to S. cerevisiae cells. S. cerevisiae possesses several protective systems against ...
Hexose Monophosphate Shunt (HMP Shunt)
... HMP shunt (PPP) is less active in skeletal muscle & non-lactating mammary glands Site:- ...
... HMP shunt (PPP) is less active in skeletal muscle & non-lactating mammary glands Site:- ...
Molecular cloning and biochemical characterization of a
... hydroperoxidation of polyunsaturated fatty acids (PUFA) containing a cis,cis-1,4 pentadiene moiety. Hydroperoxidation products derived from LOX activity are rapidly converted into a number of compounds involved in plant defence, senescence, seed germination, plant growth and development. In particul ...
... hydroperoxidation of polyunsaturated fatty acids (PUFA) containing a cis,cis-1,4 pentadiene moiety. Hydroperoxidation products derived from LOX activity are rapidly converted into a number of compounds involved in plant defence, senescence, seed germination, plant growth and development. In particul ...
Fumaric acid: an overlooked form of fixed carbon in Arabidopsis and
... of the column retention times of the derivatized products of succinic acid, alpha-ketoglutaric acid and maleic acid (Table 1) with the column retention times of all major compounds produced by derivatization of Arabidopsis leaf material (Fig. 1) revealed that Arabidopsis leaves do not contain substa ...
... of the column retention times of the derivatized products of succinic acid, alpha-ketoglutaric acid and maleic acid (Table 1) with the column retention times of all major compounds produced by derivatization of Arabidopsis leaf material (Fig. 1) revealed that Arabidopsis leaves do not contain substa ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... numbers refer to the position on the genomic HIV-1 RNA transcript, with +1 being the capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; ...
... numbers refer to the position on the genomic HIV-1 RNA transcript, with +1 being the capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; ...
Lipids- Structure and Metabolism
... β-oxidation. For breakdown of fatty acid to acetyl CoA in mitochondria. Produces ATP. De novo synthesis of fatty acids: From acetyl CoA by fatty acid synthetase complex. Occurs in cytoplasm. Triglyceride synthesis: From glycerol-3-phosphate + fatty acyl CoA. In liver and adipose tissue synthesis use ...
... β-oxidation. For breakdown of fatty acid to acetyl CoA in mitochondria. Produces ATP. De novo synthesis of fatty acids: From acetyl CoA by fatty acid synthetase complex. Occurs in cytoplasm. Triglyceride synthesis: From glycerol-3-phosphate + fatty acyl CoA. In liver and adipose tissue synthesis use ...
Questions & Answer keys Test 2 Genetic engg.
... According to the signal hypothesis, the signal peptide for secretory protein is Select one: a. rich in hydrophobic amino acids at the centre of the signal peptide and is attached to the N-terminus of the protein b. rich in hydrophilic amino acids at the centre of the signal peptide and is attached t ...
... According to the signal hypothesis, the signal peptide for secretory protein is Select one: a. rich in hydrophobic amino acids at the centre of the signal peptide and is attached to the N-terminus of the protein b. rich in hydrophilic amino acids at the centre of the signal peptide and is attached t ...
Control of Fatty-Acid Biosynthesis by Long
... biosynthesis. This mechanism avoids all complications due to unspecific associations of palmitoyl-CoA with soluble proteins of the cytoplasm (detergent properties of palmitoyl-CoA). Acetyl-CoA carboxylase competes with the lipid membrane for the longchain acyl CoAs. (The transfer of palmitoyl-CoA be ...
... biosynthesis. This mechanism avoids all complications due to unspecific associations of palmitoyl-CoA with soluble proteins of the cytoplasm (detergent properties of palmitoyl-CoA). Acetyl-CoA carboxylase competes with the lipid membrane for the longchain acyl CoAs. (The transfer of palmitoyl-CoA be ...
Comparing the Prevalence of Verotoxin
... Multiplex PCR uses probes and molecular beacons to select specific DNA regions from specific pathogens and amplify them simultaneously. As amplification increases, so does the fluorescence of the probes, allowing for the quantitative measurement of multiple pathogens. ...
... Multiplex PCR uses probes and molecular beacons to select specific DNA regions from specific pathogens and amplify them simultaneously. As amplification increases, so does the fluorescence of the probes, allowing for the quantitative measurement of multiple pathogens. ...
Preview Sample 2
... 15) ________ are molecules composed of a glycerol and three fatty acids. A) Phospholipids B) Saturated fatty acids C) Eicosanoids D) Steroids E) Triglycerides Answer: E Diff: 3 Page Ref: 21 16) A fatty acid that contains three double bonds in its carbon chain is said to be ________. A) saturated B) ...
... 15) ________ are molecules composed of a glycerol and three fatty acids. A) Phospholipids B) Saturated fatty acids C) Eicosanoids D) Steroids E) Triglycerides Answer: E Diff: 3 Page Ref: 21 16) A fatty acid that contains three double bonds in its carbon chain is said to be ________. A) saturated B) ...
Abundant RNA editing sites of chloroplast protein
... cDNAs, we identified 255 editing sites in 82 proteincoding genes in the G. biloba chloroplast genome, and all of the editing sites were C-to-U conversions. Among the 255 editing sites, ycf3 (407 and 408 bp, nucleotide position in the gene’s coding sequence), psbB (1391 and 1392 bp), rps14 (193 and 1 ...
... cDNAs, we identified 255 editing sites in 82 proteincoding genes in the G. biloba chloroplast genome, and all of the editing sites were C-to-U conversions. Among the 255 editing sites, ycf3 (407 and 408 bp, nucleotide position in the gene’s coding sequence), psbB (1391 and 1392 bp), rps14 (193 and 1 ...
a guide-book to biochemistry
... FP or DPN or ATP in biochemical language, and wherever such abbreviations are met with it is advisable-at a first glance-to remember them and the type of process they enter into, even at the expense of forgetting exactly what they represent. As to the other device, the pattern of many reactions is m ...
... FP or DPN or ATP in biochemical language, and wherever such abbreviations are met with it is advisable-at a first glance-to remember them and the type of process they enter into, even at the expense of forgetting exactly what they represent. As to the other device, the pattern of many reactions is m ...
23 Amino Acids, Peptides, and Proteins
... occurring polymers of sugar subunits (Section 22.18), and nucleic acids are covered in Chapter 27. We will now look at proteins and the structurally similar, but shorter, peptides. Peptides and proteins are polymers of amino acids linked together by amide bonds. The repeating units are called amino ...
... occurring polymers of sugar subunits (Section 22.18), and nucleic acids are covered in Chapter 27. We will now look at proteins and the structurally similar, but shorter, peptides. Peptides and proteins are polymers of amino acids linked together by amide bonds. The repeating units are called amino ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... CODEHOP (Rose et al 1998, 2003; Rose 2005) CODEHOP stands for “Consensus-degenerate hybrid oligonucleotide primer.” The CODEHOP program brings together two previous solutions for primer design for “distantly related” input sequences and combines them into one, “hybrid” primer [11]. One of these solu ...
... CODEHOP (Rose et al 1998, 2003; Rose 2005) CODEHOP stands for “Consensus-degenerate hybrid oligonucleotide primer.” The CODEHOP program brings together two previous solutions for primer design for “distantly related” input sequences and combines them into one, “hybrid” primer [11]. One of these solu ...
Prediction and investigation of novel proteins in DNA double
... causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis or cell death. DSBs are primarily repaired by two independent and highly conserved pathways: homologous recombination (HR) and non-homologous e ...
... causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis or cell death. DSBs are primarily repaired by two independent and highly conserved pathways: homologous recombination (HR) and non-homologous e ...
2. Solid-phase peptide synthesis (SPPS) - RSC Publishing
... 1. Introduction Peptide synthesis has been, for more than a century, a significant synthetic approach in different areas such as protein chemistry and organic synthesis. Basically, the peptide ...
... 1. Introduction Peptide synthesis has been, for more than a century, a significant synthetic approach in different areas such as protein chemistry and organic synthesis. Basically, the peptide ...
video slide - Dublin City Schools Home
... A Closer Look: Obtaining the Gene of Interest • How can a researcher obtain DNA that encodes a particular gene of interest? • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This coll ...
... A Closer Look: Obtaining the Gene of Interest • How can a researcher obtain DNA that encodes a particular gene of interest? • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This coll ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.