Transcription factors Oct-1 and NF-YA regulate the p53
... agent. (a) Summary of results for the GADD45 promoter reporter constructs containing the indicated mutations either in OCT-1 sites or in CAAT box. After cells were transfected with the indicated constructs, cells were treated with MMS and UV radiation. The CAT assays were performed and the CAT activ ...
... agent. (a) Summary of results for the GADD45 promoter reporter constructs containing the indicated mutations either in OCT-1 sites or in CAAT box. After cells were transfected with the indicated constructs, cells were treated with MMS and UV radiation. The CAT assays were performed and the CAT activ ...
Ionic distribution around simple DNA models. I
... the DNA grooved structure in a simplified manner is introduced. From a methodological point of view, special attention is paid to the treatment of long-range forces along the axial direction. Exact formulas for discretely charged polyelectrolytes are used. Regarding the density profile results, it i ...
... the DNA grooved structure in a simplified manner is introduced. From a methodological point of view, special attention is paid to the treatment of long-range forces along the axial direction. Exact formulas for discretely charged polyelectrolytes are used. Regarding the density profile results, it i ...
GeneMorph II EZClone Domain Mutagenesis Kit
... denatured and annealed to the original donor plasmid and extended with a specialized enzyme mix containing a high fidelity DNA polymerase. Using a high-fidelity polymerase minimizes unwanted secondary mutations during the cloning process, which can affect downstream results. The EZClone reaction is ...
... denatured and annealed to the original donor plasmid and extended with a specialized enzyme mix containing a high fidelity DNA polymerase. Using a high-fidelity polymerase minimizes unwanted secondary mutations during the cloning process, which can affect downstream results. The EZClone reaction is ...
Identification of Bacterial Species Using Colony PCR
... did not permit for the bacteria to be sent off for sequencing to see if each group had predicted the correct species of bacterium. Plates streaked with each group’s bacterium were stored in the refrigerator. The goal of my part of the project was to take each group’s bacteria, amplify the 16S rRNA g ...
... did not permit for the bacteria to be sent off for sequencing to see if each group had predicted the correct species of bacterium. Plates streaked with each group’s bacterium were stored in the refrigerator. The goal of my part of the project was to take each group’s bacteria, amplify the 16S rRNA g ...
Biosynthesis of Plant Secondary metabolites
... cymarose as in cardiac glycosides. More than one molecule of such sugars may be attached to the aglycone either by separate linkages, which is rare, or, more commonly, as di-, tri- or tetrasaccharide. Such complex glycosides are formed by stepwise addition of sugars to aglycone. Since sugars exist i ...
... cymarose as in cardiac glycosides. More than one molecule of such sugars may be attached to the aglycone either by separate linkages, which is rare, or, more commonly, as di-, tri- or tetrasaccharide. Such complex glycosides are formed by stepwise addition of sugars to aglycone. Since sugars exist i ...
From DNA to Disorder - Liberty Union High School District
... who inherit one copy of the gene. Although achondroplasia can be inherited as a dominant trait, approximately 80% of the cases are due to new point mutations (see sidebar). Approximately 98% of all cases of achondroplasia are due to a G to A substitution at the 1138th nucleotide with the remaining 2 ...
... who inherit one copy of the gene. Although achondroplasia can be inherited as a dominant trait, approximately 80% of the cases are due to new point mutations (see sidebar). Approximately 98% of all cases of achondroplasia are due to a G to A substitution at the 1138th nucleotide with the remaining 2 ...
Back-translation Using First Order Hidden Markov Models
... of the same species of plant), we expected a minuscule error rate in our backtranslated output in each of the three scenarios. Further, we predicted that the Spanish training set would give the most accurate back-translation of the three sets and the Russian set would give the least accurate. Surpr ...
... of the same species of plant), we expected a minuscule error rate in our backtranslated output in each of the three scenarios. Further, we predicted that the Spanish training set would give the most accurate back-translation of the three sets and the Russian set would give the least accurate. Surpr ...
Slide 1
... Large numbers of identical bacteria, each containing the inserted DNA molecules, can be produced through a process called cloning. ...
... Large numbers of identical bacteria, each containing the inserted DNA molecules, can be produced through a process called cloning. ...
Carbohydrates
... they are sugars or are polymers of sugars or monosaccharides examples glucose, fructose, starch, glycogen, sugar and cellulose. Molecular Structure of Carbohydrate: Carbohydrates are represented by a general formula Cn (H 2 O)n, where n represents number of carbon atoms. Chemically carbohydrates are ...
... they are sugars or are polymers of sugars or monosaccharides examples glucose, fructose, starch, glycogen, sugar and cellulose. Molecular Structure of Carbohydrate: Carbohydrates are represented by a general formula Cn (H 2 O)n, where n represents number of carbon atoms. Chemically carbohydrates are ...
Searching for Novel Riboswitches in Newly Sequenced Genomes
... Prokaryotic Transcription Termination by Riboswitches ...
... Prokaryotic Transcription Termination by Riboswitches ...
structure and mechanism of dna polymerases
... every 109–1010 bases replicated (Echols and Goodman, 1991). This remarkable accuracy is achieved by a combination of different mechanisms working in unison. The initial discrimination is carried out during the nucleotide incorporation stage in which a DNA polymerase accurately selects a nucleotide ( ...
... every 109–1010 bases replicated (Echols and Goodman, 1991). This remarkable accuracy is achieved by a combination of different mechanisms working in unison. The initial discrimination is carried out during the nucleotide incorporation stage in which a DNA polymerase accurately selects a nucleotide ( ...
Answers #2
... coding capacity (but you cannot be certain from just these data & are not being asked for that distinction in the question). Given the qualifiers of strong bands and high stringency in the question, it is less likely, but not impossible, that one (or both) of the bands corresponds to mRNAs of a rela ...
... coding capacity (but you cannot be certain from just these data & are not being asked for that distinction in the question). Given the qualifiers of strong bands and high stringency in the question, it is less likely, but not impossible, that one (or both) of the bands corresponds to mRNAs of a rela ...
H 3 O +
... – His has a side chain pKa of 6.0 and is only 10% protonated at pH 7 – Because His has a pKa near neutral, it plays important roles as a proton donor or acceptor in many enzymes. – His containing peptides are important biological buffers ...
... – His has a side chain pKa of 6.0 and is only 10% protonated at pH 7 – Because His has a pKa near neutral, it plays important roles as a proton donor or acceptor in many enzymes. – His containing peptides are important biological buffers ...
Scheme I Supplementary Material Available: Detailed
... make an efficient stereospecific synthesis of these analogues highly desirable. Most previous syntheses of 1 have involved reduction of a-diazo ester^,^ nitrosation and reduction of a-amino acids,1° Hofmann rearrangement of a-ureido acids,*," or treatment of a-halo carboxylic acids with hydrazine.I2 ...
... make an efficient stereospecific synthesis of these analogues highly desirable. Most previous syntheses of 1 have involved reduction of a-diazo ester^,^ nitrosation and reduction of a-amino acids,1° Hofmann rearrangement of a-ureido acids,*," or treatment of a-halo carboxylic acids with hydrazine.I2 ...
foreign
... Add part totals of the question and write the question total at the end. Count all the ticks for the entire question as a recheck and draw a circle around the question total to confirm correct addition. ...
... Add part totals of the question and write the question total at the end. Count all the ticks for the entire question as a recheck and draw a circle around the question total to confirm correct addition. ...
Operon Info_pGLO pre lab
... binding to the operator and blocking RNA polymerase The repressor is the product of a separate regulatory gene Repressors are often made continuously by the cell, at low levels © 2014 Pearson Education, Inc. ...
... binding to the operator and blocking RNA polymerase The repressor is the product of a separate regulatory gene Repressors are often made continuously by the cell, at low levels © 2014 Pearson Education, Inc. ...
b-oxidation - mustafaaltinisik.org.uk
... Deficiencies of carnitine or carnitine transferase or translocator activity are related to disease state • Symptons include muscle cramping during exercise, severe weakness and death. • Affects muscles, kidney, and heart tissues. • Muscle weakness related to importance of fatty acids as long term e ...
... Deficiencies of carnitine or carnitine transferase or translocator activity are related to disease state • Symptons include muscle cramping during exercise, severe weakness and death. • Affects muscles, kidney, and heart tissues. • Muscle weakness related to importance of fatty acids as long term e ...
Hyaluronic Acid in Dermatology and Dermocosmetics
... in this period the production of collagen, elastin and hyaluronic acid slows down; cell exchange becomes slower, and the thickness of the corneal layer increases. This all leads to the appearance of classic signs of ageing: wrinkles, laughter lines, loss of hydration, tone and elasticity. The speed ...
... in this period the production of collagen, elastin and hyaluronic acid slows down; cell exchange becomes slower, and the thickness of the corneal layer increases. This all leads to the appearance of classic signs of ageing: wrinkles, laughter lines, loss of hydration, tone and elasticity. The speed ...
Simplification of the genetic code: restricted
... is utilized by almost all organisms, this code had been considered as ‘universal’ (1). The genetic code, however, was found to be malleable, with the discovery of deviant codes (2–4). For example, CUG codons are assigned to serine (Ser), instead of leucine (Leu), in Candida cylindracea. In laborator ...
... is utilized by almost all organisms, this code had been considered as ‘universal’ (1). The genetic code, however, was found to be malleable, with the discovery of deviant codes (2–4). For example, CUG codons are assigned to serine (Ser), instead of leucine (Leu), in Candida cylindracea. In laborator ...
HS-SCI-APB-Unit 3 -- Chapter 16- Molecular Basis
... this newly acquired trait of pathogenicity was inherited by all the descendants of the transformed bacteria. Clearly, some chemical component of the dead pathogenic cells caused this heritable change, although the identity of the substance was not known. Griffith called the phenomenon transformation ...
... this newly acquired trait of pathogenicity was inherited by all the descendants of the transformed bacteria. Clearly, some chemical component of the dead pathogenic cells caused this heritable change, although the identity of the substance was not known. Griffith called the phenomenon transformation ...
Amino acid Catabolism
... The amino acids recovered from protein turnover, or obtained from the diet or de novo synthesis, are used to : • Support ongoing protein synthesis in cells. • they are also used as metabolic precursors for numerous biomolecules, including heme groups (hemoglobin and cytochromes), nucleotide bases (p ...
... The amino acids recovered from protein turnover, or obtained from the diet or de novo synthesis, are used to : • Support ongoing protein synthesis in cells. • they are also used as metabolic precursors for numerous biomolecules, including heme groups (hemoglobin and cytochromes), nucleotide bases (p ...
Copying our Genes
... Housed in the nucleus of your cells, DNA holds the key to your genetic destiny. The DNA code tells an amazing story about the person you will grow up to be. Each gene controls production of a protein, which ultimately affects a trait in your body. The tools of modern day molecular biology give scien ...
... Housed in the nucleus of your cells, DNA holds the key to your genetic destiny. The DNA code tells an amazing story about the person you will grow up to be. Each gene controls production of a protein, which ultimately affects a trait in your body. The tools of modern day molecular biology give scien ...
MTHFr, Methylation and Metals
... Methylation is related to neurotransmitter levels; methylation of intermediates in tryptophan metabolism can affect the levels of serotonin. Intermediates of the methylation pathway are also shared with the pathway involved in dopamine synthesis. Consequently, imbalances in the methylation pathway ...
... Methylation is related to neurotransmitter levels; methylation of intermediates in tryptophan metabolism can affect the levels of serotonin. Intermediates of the methylation pathway are also shared with the pathway involved in dopamine synthesis. Consequently, imbalances in the methylation pathway ...
Organic molecules (biomolecules)
... structural molecules in the cell, as energy sources, and most importantly as "enzymes", (protein catalysts which speed up chemical reactions in the cell without the need for high temperature or drastic pH changes). ...
... structural molecules in the cell, as energy sources, and most importantly as "enzymes", (protein catalysts which speed up chemical reactions in the cell without the need for high temperature or drastic pH changes). ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.