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The Microbiological Degradation of Aromatic Compounds
The Microbiological Degradation of Aromatic Compounds

... labile and difficult to separate; parts of this scheme are therefore still hypothetical. The corresponding ' diol ' intermediate has been isolated from cultures of naphthalene (Walker & Wiltshire, 1.953)and phenanthrene (Colla, Fiecchi & Treccani, 1959); evidence for its formation also exists in the ...
Site specific insertion of a type I rDNA dement into a unique
Site specific insertion of a type I rDNA dement into a unique

... rDNA. There is no significant feature within the insertion sequence that could have determined the position of the left junction with the sequence into which it is inserted. Like the corresponding short type I insertions in rDNA, the insertion into the unique DNA is flanked on both sides by a duplic ...
FCR 16 - University of Kentucky
FCR 16 - University of Kentucky

... 9, 2003, titled “TRANS-EXCISION-SPLICING RIBOZYME AND METHODS OF USE” Inventors: Dr. Stephen M. Testa and Dr. Michael A. Bell. The present invention provides a group I intron-derived ribozyme that binds RNA in trans, excises an internal segment from within the RNA, and splices the remaining 5' and 3 ...
Large Scale SNP Scanning on Human Chromosome Y and DNA
Large Scale SNP Scanning on Human Chromosome Y and DNA

... High-throughput SNP scanning is an important tool for genome studies. We have used synthetic PCR constructs to demonstrate the detection of all possible SNP base changes. LCGreen Plus was included in the PCR reaction and high-resolution melting analysis was performed five minutes after amplification ...
fermentation
fermentation

... alcohol ,enzymes,single cell proteins and additives. A restricted number of yeasts are used in the fermentation. Saccharamyces spp. are used in the production of bread, wine, champania, alcohol,distilled licor and invertase enzyme. ...
technique
technique

... DNA Cloning and Its Applications: A Preview • Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their plasmids • Plasmids are small circular DNA molecules that replicate separately from the bacterial chromosome ...
Handout 14, 15 - U of L Class Index
Handout 14, 15 - U of L Class Index

... anticodon loop of the tRNA, as well as individual nucleotides in the D and the TΨC arm The interaction between enzyme and aa is less extensive, the aa is smaller, several pairs of aa are structurally similar. Errors do occur, but at a low rate. ...
BCHM 562, Biochemistry II
BCHM 562, Biochemistry II

... than that of ATP. Thus, it can be used to power the ATP synthesis from ADP. This is called substrate-level phosphorylation because the phosphate donor is a Substrate with high phosphoryl-transfer potential. ...
Principles of Life - bli-research-synbio-2014-session-2
Principles of Life - bli-research-synbio-2014-session-2

Pfx50™ DNA Polymerase - Thermo Fisher Scientific
Pfx50™ DNA Polymerase - Thermo Fisher Scientific

... accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008. Phone (760) 603-7200. Fax (760) 602-6500. Email: outlicens ...
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

... deviate slightly from these ideal values. This introduces slight bends and kinks into the helix axis. ...
Shunt Pathway Significance of pentose phosphate pathway
Shunt Pathway Significance of pentose phosphate pathway

... Normally , 10% of glucose is metabolized through this pathway which operates in the cytoplasm . Energy ( ATP ) is not generated nor required . ...
Giant chromosomes
Giant chromosomes

... • The paired chromosomes of oocytes in meiosis consist of numerous chromatin loops arranged along an axis . Chiasma formation is visible at various locations. • Each segment of a lampbrush chromosome consists of a series of chromatin loops, originating from an axis and a condensed structure, the chr ...
Genetics made simple
Genetics made simple

... Gametes - Cells involved in sexual reproduction, eg. sperm cell. Genes - A section of DNA (a sequence of nucleotides) that produces a particular characteristic in a cell or organism. Genotype - Genetic composition of an individual Haploid - Cells containing only one set of parental chromosomes Heter ...
Investigation Of Haemoglobinopathy.
Investigation Of Haemoglobinopathy.

... genes. ► Indicated if mutations are not detectable with the preliminary screening and in difficult cases eg N HbA2 beta thal or silent beta thalassaemia. ...
Slide 1
Slide 1

... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: 1. The sugar in RNA is ribose instead of deoxyribose. 2. RNA is generally single-stranded and not double-str ...
Investigation Of Haemoglobinopathy.
Investigation Of Haemoglobinopathy.

... genes. ► Indicated if mutations are not detectable with the preliminary screening and in difficult cases eg N HbA2 beta thal or silent beta thalassaemia. ...
dna data storage - University of Pittsburgh
dna data storage - University of Pittsburgh

... Another major component of DNA data storage is its storage capacity. After the DNA has encoded the information, it safely stores the information until retrieval. In an article about how DNA storage works, G. Templeton describes the components of storage. Chromatin is a DNA protein system that makes ...
E. Transport of certain drugs
E. Transport of certain drugs

... Nucleic acids are required for the storage and expression of genetic information. There are two chemically distinct types of nucleic acids: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is present not only in chromosomes in the nucleus of eukaryotic organisms, but also in mitochondria ...
Document
Document

... Interactions between the snRNPs brings 5'- and 3'- splice sites together so lariat can form and exon ligation can occur ...
Bacteriophage lambda surface display of a bacterial biotin acceptor
Bacteriophage lambda surface display of a bacterial biotin acceptor

... posttranslational modi¢cations of proteins. We used bacteriophage lambda surface display of the biotin acceptor domain of the Klebsiella pneumoniae oxaloacetate decarboxylase K-subunit (Kp_OAD) to analyse the sequence requirements for biotinylation in vivo. This domain is known to be readily biotiny ...
NO!!!!!
NO!!!!!

... contains a zinc ion at the active site that interacts with the hydroxyl group of threonine. Valine is similar in overall structure to threonine but lacks hydroxyl group and thus is not joined to the tRNAThr. ...
Transcription factors Oct-1 and NF-YA regulate the p53
Transcription factors Oct-1 and NF-YA regulate the p53

... agent. (a) Summary of results for the GADD45 promoter reporter constructs containing the indicated mutations either in OCT-1 sites or in CAAT box. After cells were transfected with the indicated constructs, cells were treated with MMS and UV radiation. The CAT assays were performed and the CAT activ ...
A new primer set in a SRY gene for sex identification
A new primer set in a SRY gene for sex identification

PicodropTM
PicodropTM

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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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