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... differences in hybridization intensities between the reference and unknown sample. The ability to detect differences in intensity patterns were enhanced by including redundancy at each base position. Each site on the GeneChip corresponding to a base in the p53 sequence was covered by at least two pr ...
Molecules of Life - Morgan Community College
Molecules of Life - Morgan Community College

... • Tertiary structure is determined by interactions between R groups, rather than interactions between backbone constituents • These interactions between R groups include hydrogen bonds, ionic bonds, hydrophobic interactions, and van der Waals interactions • Strong covalent bonds called disulfide br ...
Functional Analysis of A Novel Splicing Mutation in The Mutase
Functional Analysis of A Novel Splicing Mutation in The Mutase

Characterization of the ves Gene, Which is Expressed at a Low
Characterization of the ves Gene, Which is Expressed at a Low

... (Ross et al., 1993), cold box (Jiang et al., 1996), and downstream box (Sprengart and Porter, 1997). Thus, we attempted to search such sequences around the ves promoter-operator region. There is a long A + T-rich sequence at positions 209 to 242, and in this sequence, TTGTAAAAAAA is located 40 to 60 ...
Chapter 20
Chapter 20

... DNA Cloning and Its Applications: A Preview • Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their plasmids • Plasmids are small circular DNA molecules that replicate separately from the bacterial chromosome ...
CHAPTER 3 ORGANIC CHEMISTRY
CHAPTER 3 ORGANIC CHEMISTRY

... Phosphate group Nitrogenous group Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
Introduction of Microarray
Introduction of Microarray

... stable ...
chapt 3
chapt 3

... Phosphate group Nitrogenous group Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

... The ionization of water can be measured by its electrical conductivity; pure water carries electrical current as H migrates toward the cathode and OH toward the anode. The movement of hydronium and hydroxide ions in the electric field is anomalously fast compared with that of other ions such as Na ...
Comparison of Genomes using High-Performance - FACOM
Comparison of Genomes using High-Performance - FACOM

... oversimplification, we now present some of the basic concepts. The interested reader can obtain further explanation in some excellent texts such as [10, 16]. DNA (deoxyribonuclei acid) is the chemical material in a cell that carries the genetic codes for living organisms. Its structure is a double h ...
BIOLOGY  SUPPORT   MATERIAL
BIOLOGY SUPPORT MATERIAL

... Haplontic life cycle- zygote (2n) divides by meiosis to form haploid (n) spores. Diplontic life-cycle- zygote (2n) divides mitotically, develops into embryo (2n). Oviparous animals lay eggs out-side the female body. Eggs can be fertilized/ unfertilized. Fertilized eggs covered which hard calcareous ...
Water Soluble Vitamins نسح انيز .د
Water Soluble Vitamins نسح انيز .د

... 6. Fatty acyl-CoA formation point for the oxidation of fatty acid or for their incorporation into triglycerides or phospholipids.  RDA: 5-15 mg/day  Clinical correlation:  Deficiency: due to the widespread of this vitamin, its deficiency is very rare. 6. Biotin (Vitamin H):  Structure: It is a h ...
Methods for detection of point mutations
Methods for detection of point mutations

... Principle. Under certain conditions, single-stranded (ss) nucleic acids form secondary structures in solution. The secondary structure depends on the base composition and may be altered by a single nucleotide exchange, causing differences in electrophoretic mobility under nondenaturing conditions [1 ...
Genetics and Genomics of Core Short Tandem Repeat Loci
Genetics and Genomics of Core Short Tandem Repeat Loci

... (1) Less polymorphic loci have lower mutation rates, which can make them more useful in some parentage testing situations (2) Two or three moderately polymorphic STR loci on separate chromosomes would be more powerful when the product rule was applied and would easily fit into the same PCR product s ...
Chapter 10
Chapter 10

... consists of a sugar-phosphate backbone, with nucleotides attached to the 1' carbon of the sugar The differences between DNA and RNA are that – RNA has a hydroxyl group on the 2' carbon of the sugar (the difference between deoxyribonucleic acid and ribonucleic acid) – RNA uses the pyrimidine base ura ...
Functional lipidomics of oxidized products from polyunsaturated fatty
Functional lipidomics of oxidized products from polyunsaturated fatty

... spontaneous cleavage into hydroxy-alkenals. In that case, the various hydroxy-eicosatetraenoic acids (HETEs) from ARA and hydroxy-octadecadienoic acids (HODEs) from linoleic acid can easily be measured (Figure 3). As an example of clinical investigation, 9- and 13-HODE have been found as the main hy ...
A Primer on Gene Regulation
A Primer on Gene Regulation

... (The grouping of genes into operons is common in bacteria but rare in eukaryotes.) The lactose or lac operon contains three genes: lacZ, lacY, and lacA but we will only be concerned with the most promoter-proximal gene, lacZ, which encodes β-galactosidase. β-galactosidase is an enzyme that enables E ...
Product Information: Phusion U Green Multiplex PCR Master
Product Information: Phusion U Green Multiplex PCR Master

Document
Document

... from its parents before birth through DNA Species=a type of living things that can interbreed. Offspring=babies/children Acquired trait=a trait that a living thing picks up while growing up Behaviors= ways of acting Instincts=inherited behavior Migrate=move from one place to another Hibernate=when a ...
Slide 1
Slide 1

...  Large numbers of identical bacteria, each containing the inserted DNA molecules, can be produced through a process called cloning. ...
Amino Acids
Amino Acids

2014 Human Biology Higher (Revised) Finalised Marking
2014 Human Biology Higher (Revised) Finalised Marking

... 3) They present antigens on their surface to stimulate the specific immune response. 4) They release cytokines which stimulate the specific immune response / attract more phagocytes. 5) They engulf antigen/antibody complexes (produced by B-lymphocytes). Any 2 from 5 ...
Nucleotide Sequence Preservation of Human
Nucleotide Sequence Preservation of Human

... tions? We have examined mtDNA3 isolated from neoplastic cells of patients with leukemia. We chose to study the mtDNA of human leukemic cells for 3 reasons: (a) mtDNA is well charac terized; its nucleotide sequence is known in entirety (3), and a great deal is known about between-individual nucleotid ...
Are you ready for S317?
Are you ready for S317?

... (a) Disulfide bonds stabilise folded subunits (i.e. tertiary structure) but do not play a role in quaternary structure. (b) A polypeptide will fold such that it adopts the most stable conformation. (c) Cytosolic polypeptides fold such that their hydrophobic residues are exposed on their surface. (d) ...
Fatty Acids - Mayo Clinic
Fatty Acids - Mayo Clinic

... HIV-1 RNA quantitation is performed by PCR using the Roche Amplicor System. Plasma is chemically extracted and the viral RNA is precipitated with isopropanol. A known amount of a standard synthetic RNA molecule is added to each specimen to permit quantitation of HIV RNA by a comparison of resulting ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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