Central Dogma Review Sheet
... *Review the structure of proteins. You should know the relationship of amino acid to proteins, and know what a peptide bond is. Review also enzymes, particularly the importance of enzyme shape (particularly the active site) to its function. 1. Be able to describe the structure of DNA, including the ...
... *Review the structure of proteins. You should know the relationship of amino acid to proteins, and know what a peptide bond is. Review also enzymes, particularly the importance of enzyme shape (particularly the active site) to its function. 1. Be able to describe the structure of DNA, including the ...
Detailed History - Aggie Horticulture
... 1946 Max Delbruck and Alfred Day Hershey independently discovered that the genetic material from different viruses can be combined to form a new type of virus. This process was another example of genetic recombination. 1947 Barbara McClintock first reported on "transposable elements" - known today a ...
... 1946 Max Delbruck and Alfred Day Hershey independently discovered that the genetic material from different viruses can be combined to form a new type of virus. This process was another example of genetic recombination. 1947 Barbara McClintock first reported on "transposable elements" - known today a ...
FSci Ch 07 - evansforensics
... urine, semen, and hair is examined for the presence of inherited traits. z What examples can you give of how laboratory techniques used in forensics were originally developed for other purposes? ...
... urine, semen, and hair is examined for the presence of inherited traits. z What examples can you give of how laboratory techniques used in forensics were originally developed for other purposes? ...
SNP discovery
... SSR markers have some disadvantages • High polymorphism rate, but having “many alleles” makes precise scoring difficult • SSR data is “difficult to merge across labs and groups” ...
... SSR markers have some disadvantages • High polymorphism rate, but having “many alleles” makes precise scoring difficult • SSR data is “difficult to merge across labs and groups” ...
Media:GWAS_lecture__Nov_2011_SB
... with (ten-)thousands of samples have identified a few (dozen) candidate loci with highly significant associations • Many of these associations have been replicated in independent studies ...
... with (ten-)thousands of samples have identified a few (dozen) candidate loci with highly significant associations • Many of these associations have been replicated in independent studies ...
Quizzes
... ___________ produces sugar and oxygen from carbon dioxide and water; _____________ produces carbon dioxide and water from sugar and oxygen. Together they form a closed cycle. ...
... ___________ produces sugar and oxygen from carbon dioxide and water; _____________ produces carbon dioxide and water from sugar and oxygen. Together they form a closed cycle. ...
2008 exam with answers
... Short Explanation: 5’ to 3’ exo activity is what is essential, and this activity is needed to degrade the primer Long Explanation: Results show you can replicate DNA without the 3’ to 5’ exo or polymerase activity of enzyme X, but not without the 5’ to 3’ exo. Therefore it must be the 5’ to 3’ exo o ...
... Short Explanation: 5’ to 3’ exo activity is what is essential, and this activity is needed to degrade the primer Long Explanation: Results show you can replicate DNA without the 3’ to 5’ exo or polymerase activity of enzyme X, but not without the 5’ to 3’ exo. Therefore it must be the 5’ to 3’ exo o ...
DNA Structure and history10
... • DNA composition: “Chargaff’s rules” – varies from species to species – all 4 bases not in equal quantity – bases present in characteristic ratio • humans: A = 30.9% T = 29.4% G = 19.9% C = 19.8% ...
... • DNA composition: “Chargaff’s rules” – varies from species to species – all 4 bases not in equal quantity – bases present in characteristic ratio • humans: A = 30.9% T = 29.4% G = 19.9% C = 19.8% ...
Chapter 6
... in RNA and deoyribose in DNA) and one to three phosphate molecules (Figure 6.1a). The nitrogenous bases can be grouped by structure (Figure 6.1b), into purines (adenine and guanine) and pyrimidines (thymine, cytosine, and uridine in RNA alone). In the DNA backbone, the sugar and phosphate alternate ...
... in RNA and deoyribose in DNA) and one to three phosphate molecules (Figure 6.1a). The nitrogenous bases can be grouped by structure (Figure 6.1b), into purines (adenine and guanine) and pyrimidines (thymine, cytosine, and uridine in RNA alone). In the DNA backbone, the sugar and phosphate alternate ...
KAN GRUPLARININ MOLEKÜLER YAPISI
... 1. Purpose:- to amplify (bulk up) a small amount of DNA by inserting it into in a fast growing cell e.g. bacterium, so as bacterium divides we will have many copies of our DNA 2. 1. Obtain a DNA vector which can replicate inside a bacterial cell (plasmid or virus) which 3. 2. Insert DNA into vector ...
... 1. Purpose:- to amplify (bulk up) a small amount of DNA by inserting it into in a fast growing cell e.g. bacterium, so as bacterium divides we will have many copies of our DNA 2. 1. Obtain a DNA vector which can replicate inside a bacterial cell (plasmid or virus) which 3. 2. Insert DNA into vector ...
DNA
... The nucleosomes, which at this point resemble beads on a string, are further compacted into a helical shape, called a solenoid. The solenoid defines the packing of DNA as a 30 nm fiber of chromatine and results from the helical winding of nucleosome strands. ...
... The nucleosomes, which at this point resemble beads on a string, are further compacted into a helical shape, called a solenoid. The solenoid defines the packing of DNA as a 30 nm fiber of chromatine and results from the helical winding of nucleosome strands. ...
SOP 105: Procedures for DNA gel electrophoresis.
... D: Sample contaminated with bacterial chromosomal DNA (uppermost band). E: EcoRI digestion of a sample contaminated with bacterial genomic DNA, which gives a smear above the plasmid DNA. With large-constructs such as BAC, PAC, and P1 DNA, the supercoiled form migrates at a slower rate than the linea ...
... D: Sample contaminated with bacterial chromosomal DNA (uppermost band). E: EcoRI digestion of a sample contaminated with bacterial genomic DNA, which gives a smear above the plasmid DNA. With large-constructs such as BAC, PAC, and P1 DNA, the supercoiled form migrates at a slower rate than the linea ...
Ligation and Transformation
... 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
... 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
Chapter 05 Lecture PowerPoint
... separate substances according to their sizes • Gel filtration chromatography uses columns filled with porous resins that let in smaller substances but exclude larger ones • Affinity chromatography is a powerful purification technique that exploits an affinity reagent with strong and specific affinit ...
... separate substances according to their sizes • Gel filtration chromatography uses columns filled with porous resins that let in smaller substances but exclude larger ones • Affinity chromatography is a powerful purification technique that exploits an affinity reagent with strong and specific affinit ...
Introduction to DNA Function and transcription
... What changes occur to a salmon when it spawns? ...
... What changes occur to a salmon when it spawns? ...
which came first- the chicken (dna ) or the egg (rna)?
... where life came from. With DNA, one must answer the question of where did all the information, stored within itself, come from? In other words, how could the process of natural selection or microevolution gain and pass on information to increase complexity? Today, very few scientists believe DNA cou ...
... where life came from. With DNA, one must answer the question of where did all the information, stored within itself, come from? In other words, how could the process of natural selection or microevolution gain and pass on information to increase complexity? Today, very few scientists believe DNA cou ...
DNA Fingerprinting
... We will be looking at a young woman who is suspected to have the Li-Fraumeni syndrome. The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNKA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a ...
... We will be looking at a young woman who is suspected to have the Li-Fraumeni syndrome. The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNKA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a ...
Sickle cell / mutations
... 2. Unlike popular misconceptions about people with green skin or extra body parts, a mutation is simply a change in the nucleotide sequence, or base pair sequence, of DNA. Most mutations are either neutral (they have no effect) or harmful, but occasionally mutations can actually cause a helpful chan ...
... 2. Unlike popular misconceptions about people with green skin or extra body parts, a mutation is simply a change in the nucleotide sequence, or base pair sequence, of DNA. Most mutations are either neutral (they have no effect) or harmful, but occasionally mutations can actually cause a helpful chan ...
Nick Translation DNA Labeling Systems
... of modified nucleotide analogs in the synthetic reactions results in chemically modified DNA. In one hour to overnight reactions, newly synthesized labeled DNA is generated in high yield. Random primed labeling reactions are optimized for generating maximum amounts of labeled DNA from 50 ng to 1 µg ...
... of modified nucleotide analogs in the synthetic reactions results in chemically modified DNA. In one hour to overnight reactions, newly synthesized labeled DNA is generated in high yield. Random primed labeling reactions are optimized for generating maximum amounts of labeled DNA from 50 ng to 1 µg ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.