chapter 20: dna technology and genomics
... Viruses can also be used to make a library: pieces of foreign DNA can be inserted into the virus’s genome using a restriction enzyme and ligase. This then is packaged in a capsid and allowed to infect cells. So as the virus’ DNA replicates, so does the foreign DNA of interest. ...
... Viruses can also be used to make a library: pieces of foreign DNA can be inserted into the virus’s genome using a restriction enzyme and ligase. This then is packaged in a capsid and allowed to infect cells. So as the virus’ DNA replicates, so does the foreign DNA of interest. ...
Biotechnology PPT
... Lab AP Lab 6 #88 V 8231 Any transformation will work. Connie Wood (East Meck) suggests using Carolina Biological’s “Outbreak” activity. She gets free DNA for this. ...
... Lab AP Lab 6 #88 V 8231 Any transformation will work. Connie Wood (East Meck) suggests using Carolina Biological’s “Outbreak” activity. She gets free DNA for this. ...
幻灯片 1 - University of Texas at Austin
... •The police use the same analysis to determine the identity of a person at a crime scene. After collecting a DNA samples from the scene and any suspects, the police amplify the VNTRs and digest the DNA with a restriction enzyme. •The samples are run on an agarose gel, and the bands found at the crim ...
... •The police use the same analysis to determine the identity of a person at a crime scene. After collecting a DNA samples from the scene and any suspects, the police amplify the VNTRs and digest the DNA with a restriction enzyme. •The samples are run on an agarose gel, and the bands found at the crim ...
Chapter 20: Biotechnology 11/18/2015
... 1) source of target DNA template 2) artificial primers “flanking” DNA of interest 3) heat-stable DNA polymerase (from hyperthermophile) 4) dNTP’s 5) automated thermocycler to facilitate repeated: • denaturation of DNA (separating the 2 strands) • hybridization of primers to template • DNA synthesis ...
... 1) source of target DNA template 2) artificial primers “flanking” DNA of interest 3) heat-stable DNA polymerase (from hyperthermophile) 4) dNTP’s 5) automated thermocycler to facilitate repeated: • denaturation of DNA (separating the 2 strands) • hybridization of primers to template • DNA synthesis ...
SBI4U- Molecular Genetics
... In Europeans, it has been found that Type I diabetes is more likely to occur in people who have only low number repeats (<50 repeats) at a particular gene locus (near the insulin gene) compared to those people who have at least one high number repeat (200 repeats). Below is a gel electrophoresis pat ...
... In Europeans, it has been found that Type I diabetes is more likely to occur in people who have only low number repeats (<50 repeats) at a particular gene locus (near the insulin gene) compared to those people who have at least one high number repeat (200 repeats). Below is a gel electrophoresis pat ...
molecular biology first and second lecture Introduction and brief history
... • Bacteria :Prokaryotes unicellular free living cells .only one single chromosome not enclosed inside nucleus but it is free within the cytoplasm called nucleoid .the size of Escherichia coli(E.coli) is about 4,639,221 base pair (bp )زوج قاعدةor 4.6 Kbp ( )كيلو زوج قاعة نتروجينة.it represent the ...
... • Bacteria :Prokaryotes unicellular free living cells .only one single chromosome not enclosed inside nucleus but it is free within the cytoplasm called nucleoid .the size of Escherichia coli(E.coli) is about 4,639,221 base pair (bp )زوج قاعدةor 4.6 Kbp ( )كيلو زوج قاعة نتروجينة.it represent the ...
Word Work File L_2.tmp
... 7. Hydrogen bonds form between adenine and thymine (two bonds), and between guanine and cytosine (three bonds). The sequence of bases is complementary but not identical. This allows to predict the sequence bases in one strand if one knows the sequence of bases in the other strand. 8. Each pair base ...
... 7. Hydrogen bonds form between adenine and thymine (two bonds), and between guanine and cytosine (three bonds). The sequence of bases is complementary but not identical. This allows to predict the sequence bases in one strand if one knows the sequence of bases in the other strand. 8. Each pair base ...
Chapter 20
... DNA Cloning and Its Applications: A Preview • Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their plasmids • Plasmids are small circular DNA molecules that replicate separately from the bacterial ...
... DNA Cloning and Its Applications: A Preview • Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their plasmids • Plasmids are small circular DNA molecules that replicate separately from the bacterial ...
DNA Packing
... 3. Gel electrophoresis separates DNA molecules based on size – DNA samples placed at one end of a porous gel – Current is applied and DNA molecules move from the negative electrode toward the positive ...
... 3. Gel electrophoresis separates DNA molecules based on size – DNA samples placed at one end of a porous gel – Current is applied and DNA molecules move from the negative electrode toward the positive ...
X-Sheet 2 Protein Synthesis and DNA Fingerprinting
... DNA probes. A selection of DNA sequences within the DNA profile forms what is termed the VNTR pattern for that individual. Forensic scientists are able to compare the DNA profiles to a sample that is provided from a crime scene. DNA profiling is very accurate. Sequencing of DNA: A species is a group ...
... DNA probes. A selection of DNA sequences within the DNA profile forms what is termed the VNTR pattern for that individual. Forensic scientists are able to compare the DNA profiles to a sample that is provided from a crime scene. DNA profiling is very accurate. Sequencing of DNA: A species is a group ...
DNA Mutation
... – Mutation can result in another mutant codon or one codon that has experienced two changes (double mutation) – If mutation is at the same site, the original sequence can be returned, an effect known as back mutation – Intragenic suppression can occur and this occurs when a second mutation in the sa ...
... – Mutation can result in another mutant codon or one codon that has experienced two changes (double mutation) – If mutation is at the same site, the original sequence can be returned, an effect known as back mutation – Intragenic suppression can occur and this occurs when a second mutation in the sa ...
Evolution of DNA by celluLar automata HC Lee Department of
... • Reality is complex, but models don't have to be • Von Neumann machines - a machine capable of reproduction; the basis of life is information – Stanislaw Ulam: build the machine on paper, as a collection of cells on a lattice ...
... • Reality is complex, but models don't have to be • Von Neumann machines - a machine capable of reproduction; the basis of life is information – Stanislaw Ulam: build the machine on paper, as a collection of cells on a lattice ...
Biotechnology
... 2. A DNA fingerprint is produced using a gel electrophoresis. A gel electrophoresis is a tool that separates pieces of DNA based on size (the number of base pairs in each piece). ...
... 2. A DNA fingerprint is produced using a gel electrophoresis. A gel electrophoresis is a tool that separates pieces of DNA based on size (the number of base pairs in each piece). ...
BIOT 3 Lab 3 Handout 1
... Restriction enzymes separate or “cut” a DNA strand at a particular site, meaning each restriction enzyme is specific for a particular DNA sequence, that sequence is called the restriction or recognition site. Dependent on the enzyme, the cut in the DNA strand may either result in “blunt” ends with n ...
... Restriction enzymes separate or “cut” a DNA strand at a particular site, meaning each restriction enzyme is specific for a particular DNA sequence, that sequence is called the restriction or recognition site. Dependent on the enzyme, the cut in the DNA strand may either result in “blunt” ends with n ...
Nucleic Acids - Rubin Gulaboski
... • Maintains correct genetic information • Two strands of DNA unwind ...
... • Maintains correct genetic information • Two strands of DNA unwind ...
A DNA
... Phosphate covalently (phosphodietster bond) attached to 5’C of sugar Phosphodiester bond - Covalent bond between phosphate of one nucleotide and 3’ sugar carbon of another 9 N (purine) or 6N (pyrimidine) covalently bonded to 1C of sugar ...
... Phosphate covalently (phosphodietster bond) attached to 5’C of sugar Phosphodiester bond - Covalent bond between phosphate of one nucleotide and 3’ sugar carbon of another 9 N (purine) or 6N (pyrimidine) covalently bonded to 1C of sugar ...
DNA Detectives What is Your DNA Alias? The central dogma of
... We use four letters to code all the information contained in DNA: A, T, C and G. The letters are used in groups of three. A group is called a codon. DNA contains the information that is needed by your body to make proteins. The different proteins have specific functions, such as making our hearts, h ...
... We use four letters to code all the information contained in DNA: A, T, C and G. The letters are used in groups of three. A group is called a codon. DNA contains the information that is needed by your body to make proteins. The different proteins have specific functions, such as making our hearts, h ...
SBARS: fast creation of dotplots for DNA sequences on different
... where fi , gi are two fragments of GC-content. Note that 0 1, as fi W1 and the number of terms in the sum is equal to W2. Therefore, the distance does not depend on the sizes of the windows. For recognition of repeats, the following decision rule is used: if 5" where " is a threshold, then ...
... where fi , gi are two fragments of GC-content. Note that 0 1, as fi W1 and the number of terms in the sum is equal to W2. Therefore, the distance does not depend on the sizes of the windows. For recognition of repeats, the following decision rule is used: if 5" where " is a threshold, then ...
Genome Variant Calling: A sta>s>cal perspec>ve
... • this depends very much on the set of gene models you want to use • VariantAnnota2on package provides tools to start to inves2gate this ques2on • locateVariants func2on • predictCoding func2on ...
... • this depends very much on the set of gene models you want to use • VariantAnnota2on package provides tools to start to inves2gate this ques2on • locateVariants func2on • predictCoding func2on ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.