DNA shuffling by random fragmentation and reassembly: In
... The whole 2.7-kb plasmids containing these same LacZmarkers were also efficiently reassembled from random 100to 200-bp fragments. For reassembly of fragments derived from whole plasmids the theoretical end point is a single, large concatemeric molecule. As expected, concatemers of >20 kb were obtain ...
... The whole 2.7-kb plasmids containing these same LacZmarkers were also efficiently reassembled from random 100to 200-bp fragments. For reassembly of fragments derived from whole plasmids the theoretical end point is a single, large concatemeric molecule. As expected, concatemers of >20 kb were obtain ...
Work Day 2
... Treatment of ciprofloxacin-sensitive bacteria with the antibiotic results in cell death. ...
... Treatment of ciprofloxacin-sensitive bacteria with the antibiotic results in cell death. ...
Using a Single-Nucleotide Polymorphism to Predict
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
Protocol for inserting transgene using Tn7 (6/1/06) This system uses
... Protocol for inserting transgene using Tn7 (6/1/06) This system uses Tn7 to insert transgenes at a defined neutral site in the chromosome (attTn7). The site is highly conserved and is known to work as a Tn7 attachment site in E. coli and its relatives. The attTn7 sequence is conserved in most (all) ...
... Protocol for inserting transgene using Tn7 (6/1/06) This system uses Tn7 to insert transgenes at a defined neutral site in the chromosome (attTn7). The site is highly conserved and is known to work as a Tn7 attachment site in E. coli and its relatives. The attTn7 sequence is conserved in most (all) ...
Gel electrophoresis of restriction digest
... Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5 to 25Kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) ...
... Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5 to 25Kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) ...
DNA Notes Name_____________________________ assign
... GA Biology Standards: SB2. Students will analyze how biological traits are passed on to successive generations. a. Distinguish between DNA & RNA. b. Explain the role of DNA in storing & transmitting cellular information. d. Describe the relationships between changes in DNA and potential appearance ...
... GA Biology Standards: SB2. Students will analyze how biological traits are passed on to successive generations. a. Distinguish between DNA & RNA. b. Explain the role of DNA in storing & transmitting cellular information. d. Describe the relationships between changes in DNA and potential appearance ...
Methods S1: Vector constructions and transformation of yeast and
... AvrLm4-7 in tobacco leaves, alone or fused to eGFP. eGFP coding sequence was amplified from plasmid peGFP (Clontech, Mountain View, CA, USA) using primers pBINeGFPXbaUp (which introduces a XbaI restriction site) and pBINeGFP-SacLo (which introduces a SacI restriction site). eGFP PCR product was dige ...
... AvrLm4-7 in tobacco leaves, alone or fused to eGFP. eGFP coding sequence was amplified from plasmid peGFP (Clontech, Mountain View, CA, USA) using primers pBINeGFPXbaUp (which introduces a XbaI restriction site) and pBINeGFP-SacLo (which introduces a SacI restriction site). eGFP PCR product was dige ...
Genetic Technology
... same DNA sequences, and because all cells (except gametes) of an individual have the same DNA. ...
... same DNA sequences, and because all cells (except gametes) of an individual have the same DNA. ...
DNA
... • Rails of the ladder are represented by the alternating deoxyribose and phosphate. • The pairs of bases (cytosine-guanine or thymineadenine) form the steps. ...
... • Rails of the ladder are represented by the alternating deoxyribose and phosphate. • The pairs of bases (cytosine-guanine or thymineadenine) form the steps. ...
DNA Replication
... The genetic code is the sequence of codons in mRNA that determines the sequence of amino acids in a protein. A codon is a set of three bases in mRNA that codes for a specific amino acid. Characteristics of the genetic code: 1. The genetic code is a triplet code because three bases (one codon) specif ...
... The genetic code is the sequence of codons in mRNA that determines the sequence of amino acids in a protein. A codon is a set of three bases in mRNA that codes for a specific amino acid. Characteristics of the genetic code: 1. The genetic code is a triplet code because three bases (one codon) specif ...
F9550 - Datasheet - Sigma
... 0.1 % xylene cyanole, 20 mM EDTA, pH 8. • G-25 microspin column. • 20% denatured (7M urea) acrylamide gel and electrophoresis apparatus. • TBE Running Buffer: 89mM Tris, 2mM EDTA, 89mm Boric acid pH 8.0 • X-ray film and developing machine. ...
... 0.1 % xylene cyanole, 20 mM EDTA, pH 8. • G-25 microspin column. • 20% denatured (7M urea) acrylamide gel and electrophoresis apparatus. • TBE Running Buffer: 89mM Tris, 2mM EDTA, 89mm Boric acid pH 8.0 • X-ray film and developing machine. ...
Genomics
... repeated many times. If you have a read from a repeated sequence, how do you know which copy it is? – Some repeats are next to each other (tandem repeats) and some are scattered all over the genome (dispersed repeats). The main solution to this problem is to start with longer DNA template molecules ...
... repeated many times. If you have a read from a repeated sequence, how do you know which copy it is? – Some repeats are next to each other (tandem repeats) and some are scattered all over the genome (dispersed repeats). The main solution to this problem is to start with longer DNA template molecules ...
Page 1 AP Biology TEST #5 - Chapters 11-14, 16
... 49. Chromatin structure must be altered for gene expression to occur because A) condensed chromatin is replicated but not transcribed. B) condensed chromatin makes most DNA sequences inaccessible to the transcription complex. C) decondensed chromatin has more nucleosomes per DNA molecule. D) heteroc ...
... 49. Chromatin structure must be altered for gene expression to occur because A) condensed chromatin is replicated but not transcribed. B) condensed chromatin makes most DNA sequences inaccessible to the transcription complex. C) decondensed chromatin has more nucleosomes per DNA molecule. D) heteroc ...
software development and application in bioinformatics: single
... MAQ supports Illumina reads, and includes functions that make it able to handle next-generation sequencing data [11] and AB SOLiD (a parallel next-generation sequencing platform) data. It is limited to ungapped alignment and short length reads (max 63bps). MAQ’s performance relies on one hand in its ...
... MAQ supports Illumina reads, and includes functions that make it able to handle next-generation sequencing data [11] and AB SOLiD (a parallel next-generation sequencing platform) data. It is limited to ungapped alignment and short length reads (max 63bps). MAQ’s performance relies on one hand in its ...
Genetic polymorphisms in cytochrome C oxidase subunit
... mitochondrion organelle in mammalian cells [1]. MtDNA is small and has high copy number inside the cell and it will be easier to isolate the DNA from this organelle therefore, the first genome sequencing project has been done on this molecule [2]. Human mitochondrial DNA is a double- stranded circul ...
... mitochondrion organelle in mammalian cells [1]. MtDNA is small and has high copy number inside the cell and it will be easier to isolate the DNA from this organelle therefore, the first genome sequencing project has been done on this molecule [2]. Human mitochondrial DNA is a double- stranded circul ...
Quantitating Maxwell® Extracted DNA Samples Using the
... dsDNA System provide a fast, easy and sensitive method for determining DNA concentration. The QuantiFluor® dsDNA System provides a fluorescent DNAbinding dye that enables sensitive and specific quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The dye shows minimal binding to ...
... dsDNA System provide a fast, easy and sensitive method for determining DNA concentration. The QuantiFluor® dsDNA System provides a fluorescent DNAbinding dye that enables sensitive and specific quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The dye shows minimal binding to ...
Polymerase chain reaction (PCR) The polymerase chain reaction
... The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common ...
... The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common ...
Determination of obesity associated gene variants related
... assumption, a binomial approximation of the hypergeometric distribution was used to model the probability of false SNPs to have a specific UVAM score. Using this model, the UVAM threshold for a false-discovery rate (FDR) of 1% was calculated to be a score of 21 and 23 for obese and control pools, res ...
... assumption, a binomial approximation of the hypergeometric distribution was used to model the probability of false SNPs to have a specific UVAM score. Using this model, the UVAM threshold for a false-discovery rate (FDR) of 1% was calculated to be a score of 21 and 23 for obese and control pools, res ...
SMIC Biology
... for) proteins and some that don’t. The sequences that code for proteins are called exons (they will be expressed). The sequences that do not code for any proteins are called introns (they are found in-between the expressed sequences). Specific enzymes cut out the introns and paste together the exons ...
... for) proteins and some that don’t. The sequences that code for proteins are called exons (they will be expressed). The sequences that do not code for any proteins are called introns (they are found in-between the expressed sequences). Specific enzymes cut out the introns and paste together the exons ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.