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Comparative genomics and structural biology of the
Comparative genomics and structural biology of the

... All of this happened fast • Since all eukaryotes have these traits they must have evolved in a very short period of time • Cavalier-Smith says this is quantum evolution triggered by the mitochondrial symbiosis ...
Chapter 15: Genes and How They Work
Chapter 15: Genes and How They Work

... Crick and his colleagues reasoned that the genetic code most likely consisted of a series of blocks of information called codons, each corresponding to an amino acid in the encoded protein. They further hypothesized that the information within one codon was probably a sequence of three nucleotides s ...
Rethinking Gene Expression and Evolution (Nobel Lecture)
Rethinking Gene Expression and Evolution (Nobel Lecture)

... cells, which in C. elegans inherit PIE-1 protein, are the only cells that retain the potential to launch the developmental program again in the next generation. How do developing cells, all with the same DNA content, lock in different programs of gene expression that are stable through so many round ...
Tool box for studying gene function in neural development
Tool box for studying gene function in neural development

... • Dominant-negative protein constructs: mutated proteins that perturb normal function of the endogenous gene product. Fairly advanced knowledge of protein function required. Specificity often difficult to assess, because they inhibit may related gene products from several different genes. ...
Mapping of the RNA-binding domain of the alfalfa mosaic virus
Mapping of the RNA-binding domain of the alfalfa mosaic virus

... protein (Osman et al., 1993). In addition, alaninescanning mutations affecting several other regions of the molecule decreased the affinity of the movement protein for RNA and the cooperativity of binding; mutations in one of these regions also affected the biological function of the protein (Giesma ...
Chapter Sixteen
Chapter Sixteen

... Understand the basic structure of a tRNA molecule and how its attachment site and anticodon region contribute to its action. Appreciate that it forms an ester link to its amino acid, and that this link is hydrolyzed when the protein chain is formed. Appreciate that protein formation on the ribosome ...
Biology
Biology

... the process of transcription. The start of a gene. Poly A tail: code on DNA that tells mRNA to stop the process of transcription. The end of a gene. AUG: code on mRNA that tells the ribosome to start making a protein UAA, UAG, UGA: code on mRNA that tells the ribosome to stop making a protein Copyri ...
Diagnostic Issues
Diagnostic Issues

... Number/µL ...
Bis2A 12.2 Eukaryotic Transcription
Bis2A 12.2 Eukaryotic Transcription

... certain function. If this is the case, it would be benecial to the cell for that gene's promoter to recruit transcription factors more eciently and increase gene expression. Scientists examining the evolution of promoter sequences have reported varying results. In part, this is because it is dicu ...
Chapter 11: DNA: The Molecule of Heredity
Chapter 11: DNA: The Molecule of Heredity

... _________ is the pentose sugar on RNA. __________, not DNA can leave the nucleus through ________ in the nuclear envelope. Proteins are made at the _____________. The Blueprint of Life Every cell in your body has the same "blueprint" or the same DNA. Like the blueprints of a house tell the builders ...
Pentose sugars
Pentose sugars

... Phosphate group (acidic, negatively charged) Nitrogen containing base (1 or 2 rings) The nitrogen base and the phosphate group are linked to the pentose sugar by covalent bonds ...
DNA and Transcription Tutorial
DNA and Transcription Tutorial

... the mRNA links up with a ribosome. Once at the ribosome, the process called translation will begin. Translation is the process where a ribosome builds a protein. The details of translation will be discussed another day. For now, let’s review. ...
Document
Document

... the mRNA links up with a ribosome. Once at the ribosome, the process called translation will begin. Translation is the process where a ribosome builds a protein. The details of translation will be discussed another day. For now, let’s review. ...
Chapter 12: DNA & RNA
Chapter 12: DNA & RNA

... attachment site • Does the order of amino acids matter? Yes, they must be in order for the protein to fold correctly. ...
Chapter Sixteen - Wright State University
Chapter Sixteen - Wright State University

... actually code for different kinds of RNA molecules that help regulate the expression of genes. ■ Apparently the resulting differences in gene ...
Genetic Code
Genetic Code

... • The sequence of amino acids is coded by the mRNA • Each triplet of bases along mRNA codes for an amino acid • The triplet are called codons • Codons are known for all 20 amino acids • Some codons signal the “start” and “end” of a polypeptide chain ...
Synthetic lethal analysis of Caenorhabditis elegans posterior
Synthetic lethal analysis of Caenorhabditis elegans posterior

... RNAi of most PAL-1 targets does not result in•Identified a in phenotype. Why? Is there overlapping function? microarray screen •Validated using GFP transcriptional reporters •Many targets are TF’s ...
KEY Honors Biology Chapter 10
KEY Honors Biology Chapter 10

... only on a nutrient medium to which a certain vitamin has been added because they lack a gene that enables them to make this vitamin for themselves. It has been found that bacteria can absorb genes from other dead, ground-up bacteria. Describe an experiment using type A and type V E. coli to demonstr ...
Final Research Genetics
Final Research Genetics

... x. Gene Expression data analysis: Quantitative PCR Quantitative PCR is done to compare expression between two data samples. The control gene used was PSN0001; it has shown to be contiguously expressed in previous experiments. The control gene was used to normalize the expressed gene. In the 9 plates ...
Document
Document

... protein. The protein is the phenotype (expression of the ...
presentation (spanish ppt format, 4.7 MB)
presentation (spanish ppt format, 4.7 MB)

... messenger RNAs, code for proteins ribosomal RNAs, form the basic structure of the ribosome and catalyze protein synthesis transfer RNAs, central to protein synthesis as adaptors between mRNA and amino acids small nuclear RNAs, function in a variety of nuclear processes, including the splicing of pre ...
tRNA and Protein Building
tRNA and Protein Building

... RNA produced in the nucleus of a cell moves out of the nucleus to the cell’s ribosomes. This RNA is a specific sequence of base copied from the DNA which carries the chromosomal genetic message to the cytoplasm. Thus, it is called messenger RNA (mRNA). At the ribosomes, mRNA directs the building of ...
Jeopardy Review 2013
Jeopardy Review 2013

... single nucleotide switched with another is called a… ...
Small-Subunit Ribosomal RNA Sequence from
Small-Subunit Ribosomal RNA Sequence from

... each clone, nested deletions were produced by the Exo III/S1 technique in the two small-subunit plasmids (pNgSS6 and pNgSS55 ) by using an Erase-a-Base@ kit (Promega Biotec) and the provided protocol. Deletions of - 200 bases in size were generated by sampling at 30-s time intervals. A small number ...
Eukaryotic Transcription
Eukaryotic Transcription

... 2 Eukaryotic Elongation and Termination Following the formation of the preinitiation complex, the polymerase is released from the other transcription factors, and elongation is allowed to proceed as it does in prokaryotes with the polymerase synthesizing premRNA in the 5' to 3' direction. As discuss ...
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Nucleic acid tertiary structure



The tertiary structure of a nucleic acid is its precise three-dimensional structure, as defined by the atomic coordinates. RNA and DNA molecules are capable of diverse functions ranging from molecular recognition to catalysis. Such functions require a precise three-dimensional tertiary structure. While such structures are diverse and seemingly complex, they are composed of recurring, easily recognizable tertiary structure motifs that serve as molecular building blocks. Some of the most common motifs for RNA and DNA tertiary structure are described below, but this information is based on a limited number of solved structures. Many more tertiary structural motifs will be revealed as new RNA and DNA molecules are structurally characterized.
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