![Determination of guanine-plus-cytosine content of](http://s1.studyres.com/store/data/001205901_1-372646d7e716dad74ac192f994211e23-300x300.png)
Determination of guanine-plus-cytosine content of
... contour plots. Background fluorescence began to appear on the contour plot at 2 x lo6 c.f.u. ml-l and increased as the cell concentration decreased, such that at 2 x lo4 c.f.u. ml-l, a discrete cell population was barely discernible. At concentrations of < 2 x lo3 c.f.u. ml-l, only background fluore ...
... contour plots. Background fluorescence began to appear on the contour plot at 2 x lo6 c.f.u. ml-l and increased as the cell concentration decreased, such that at 2 x lo4 c.f.u. ml-l, a discrete cell population was barely discernible. At concentrations of < 2 x lo3 c.f.u. ml-l, only background fluore ...
Natural Transfer of Conjugative Transposon Tn916 between Gram
... gram-negative eubacteria, with subsequent expression in the new host. Although we did not make any attempt to determine the nature of the transfer process (transformation versus conjugation), there are indications that favor a conjugationlike mechanism. First, C. acetobutylicum has been reported to ...
... gram-negative eubacteria, with subsequent expression in the new host. Although we did not make any attempt to determine the nature of the transfer process (transformation versus conjugation), there are indications that favor a conjugationlike mechanism. First, C. acetobutylicum has been reported to ...
Computational Biology
... similarity (determined by FunSimMat) than that between random pairs of genes Not the case for co-methylated gene pairs on different chromosomes ...
... similarity (determined by FunSimMat) than that between random pairs of genes Not the case for co-methylated gene pairs on different chromosomes ...
DNA shuffling by random fragmentation and reassembly: In
... used at 2.5 units per 100 .1A of reaction mixture. A PCR program of 940 for 60 s; 940C for 30 s, 50-55TC for 30 s, and 72TC for 30 s (30-45 times); and 72°C for 5 min was used in an MJ Research (Cambridge, MA) PTC-150 thermocycler. The PCR reassembly of small fragments into larger sequences was anal ...
... used at 2.5 units per 100 .1A of reaction mixture. A PCR program of 940 for 60 s; 940C for 30 s, 50-55TC for 30 s, and 72TC for 30 s (30-45 times); and 72°C for 5 min was used in an MJ Research (Cambridge, MA) PTC-150 thermocycler. The PCR reassembly of small fragments into larger sequences was anal ...
DNA methylation involved in proline accumulation in - Funpec-RP
... regions of the world. Plant response to drought is a very complex network affecting almost all processes in plant metabolism and development, including water balance, nutrient uptake and metabolism, and photosynthetic assimilation. Plant survival and growth under drought conditions result via adapti ...
... regions of the world. Plant response to drought is a very complex network affecting almost all processes in plant metabolism and development, including water balance, nutrient uptake and metabolism, and photosynthetic assimilation. Plant survival and growth under drought conditions result via adapti ...
Biological information flow
... accessible to the transcription machinery. A common means of weakening the interaction of histones with DNA is by acetylation of histones on specific lysine residues. Histones can also be modified by other means, such as methylation and phosphorylation. ...
... accessible to the transcription machinery. A common means of weakening the interaction of histones with DNA is by acetylation of histones on specific lysine residues. Histones can also be modified by other means, such as methylation and phosphorylation. ...
Mutation, Mutagens, and DNA Repair
... In excision repair, the region of DNA containing the dimer or other damage is physically cut out and then replaced by new DNA synthesis (Figure 1). Excision repair has more steps and requires more enzymes than photoreactivation, but it can work on damage created by agents other than UV and on lesion ...
... In excision repair, the region of DNA containing the dimer or other damage is physically cut out and then replaced by new DNA synthesis (Figure 1). Excision repair has more steps and requires more enzymes than photoreactivation, but it can work on damage created by agents other than UV and on lesion ...
Learning About DNA
... differences among people. The cells in your body have stored a great deal of information in a very small space. They can even make an exact copy of the DNA to be passed on to new cells produced by your body. So what exactly is this thing called DNA? It’s a complex chemical substance found in the cel ...
... differences among people. The cells in your body have stored a great deal of information in a very small space. They can even make an exact copy of the DNA to be passed on to new cells produced by your body. So what exactly is this thing called DNA? It’s a complex chemical substance found in the cel ...
Discussion S1.
... Titz et al., The protein interactome of Treponema pallidum. Extended Discussion and Materials & Methods Results and Discussion An integrated view of DNA-metabolism related processes (Figure 4): Each largescale interaction study covers a given functional complex only to a limited extend. This is due ...
... Titz et al., The protein interactome of Treponema pallidum. Extended Discussion and Materials & Methods Results and Discussion An integrated view of DNA-metabolism related processes (Figure 4): Each largescale interaction study covers a given functional complex only to a limited extend. This is due ...
Protein–DNA Interactions: Amino Acid Conservation and the Effects
... We investigate the conservation of amino acid residue sequences in 21 DNA-binding protein families and study the effects that mutations have on DNA-sequence recognition. The observations are best understood by assigning each protein family to one of three classes: (i) non-specific, where binding is ...
... We investigate the conservation of amino acid residue sequences in 21 DNA-binding protein families and study the effects that mutations have on DNA-sequence recognition. The observations are best understood by assigning each protein family to one of three classes: (i) non-specific, where binding is ...
Archaeal Transcription Initiation - IMBB
... Crenarchaeota (Grayling et al., 1996). Fifteen complete archaeal histone sequences are available, and the residues on the interacting hydrophobic faces of their a helix 2s are very highly conserved (-A15 ---L14 A15--L12 --A 13--I12A 13--A14 V13 --A15--A 15- [hyphens indicate amino acid residues betw ...
... Crenarchaeota (Grayling et al., 1996). Fifteen complete archaeal histone sequences are available, and the residues on the interacting hydrophobic faces of their a helix 2s are very highly conserved (-A15 ---L14 A15--L12 --A 13--I12A 13--A14 V13 --A15--A 15- [hyphens indicate amino acid residues betw ...
letters
... (Fig. 2a, lane 2). GST–SUV39H1 was again used as a positive control (Fig. 2a, lane 7). Using various GST-fused EZH2 fragments, we found that EZH2 associates with DNMTs primarily via its amino-terminal portion (Fig. 2a, lanes 4–6). In the reciprocal experiment, IVT EZH2 was found to interact with bot ...
... (Fig. 2a, lane 2). GST–SUV39H1 was again used as a positive control (Fig. 2a, lane 7). Using various GST-fused EZH2 fragments, we found that EZH2 associates with DNMTs primarily via its amino-terminal portion (Fig. 2a, lanes 4–6). In the reciprocal experiment, IVT EZH2 was found to interact with bot ...
Identification of R-Gene Homologous DNA Fragments Genetically
... mechanisms of resistance, the interactions involved in pathogen recognition, and the evolution of the R-genes. Furthermore, cloned genes can be transferred to other species (Rommens et al. 1995; Thilmony et al. 1995) to study the resistance mechanism in a completely different genetic background. It ...
... mechanisms of resistance, the interactions involved in pathogen recognition, and the evolution of the R-genes. Furthermore, cloned genes can be transferred to other species (Rommens et al. 1995; Thilmony et al. 1995) to study the resistance mechanism in a completely different genetic background. It ...
unit – vi genetics - Sakshieducation.com
... 24. How many base pairs are observed in human genome? What is the average number of base pairs in a human gene? A. ...
... 24. How many base pairs are observed in human genome? What is the average number of base pairs in a human gene? A. ...
Complete Laboratory PDF
... the principle that the closer two genes or markers are located to one another on a chromosome, the greater the chance that they will be inherited together as a unit (linked). Conversely, locations farther apart on the chromosome are more likely to be separated by chromosome recombination during meio ...
... the principle that the closer two genes or markers are located to one another on a chromosome, the greater the chance that they will be inherited together as a unit (linked). Conversely, locations farther apart on the chromosome are more likely to be separated by chromosome recombination during meio ...
Resolvase OsGEN1 Mediates DNA Repair by
... MUS81-EME1 and SLX1-SLX4 independent pathway (Wyatt et al., 2013). Yen1/GEN1 were considered to be the canonical HJ resolvases in eukaryotes as they cleave HJs in a manner analogous to that of the Escherichia coli resolvase RuvC (crossover junction endodeoxyribonuclease RuvC; Ip et al., 2008; Rass e ...
... MUS81-EME1 and SLX1-SLX4 independent pathway (Wyatt et al., 2013). Yen1/GEN1 were considered to be the canonical HJ resolvases in eukaryotes as they cleave HJs in a manner analogous to that of the Escherichia coli resolvase RuvC (crossover junction endodeoxyribonuclease RuvC; Ip et al., 2008; Rass e ...
TRANSCRIPTION – TRANSLATION
... information into the cytoplasm, where the cell uses it to construct specific proteins, RNA synthesis is transcription; protein synthesis is translation. RNA differs from DNA in that it is single stranded, contains Uracil instead of Thymine and ribose instead of deoxyribose, and has different functio ...
... information into the cytoplasm, where the cell uses it to construct specific proteins, RNA synthesis is transcription; protein synthesis is translation. RNA differs from DNA in that it is single stranded, contains Uracil instead of Thymine and ribose instead of deoxyribose, and has different functio ...
Replication origin plasticity, Taylor-made: inhibition vs
... many more potential origins than are typically used in a normal cell cycle and these origins can be recruited to complete DNA synthesis more rapidly. However, activation of these origins required conditions in which checkpoints were inhibited, emphasizing in the words of Woodward et al., “the counte ...
... many more potential origins than are typically used in a normal cell cycle and these origins can be recruited to complete DNA synthesis more rapidly. However, activation of these origins required conditions in which checkpoints were inhibited, emphasizing in the words of Woodward et al., “the counte ...
Integrated genomic DNA/RNA profiling of
... The assay we developed uses next-generation DNA and RNA sequencing and builds on the workflow that has been optimized for genomic profiling of DNA from patients with solid tumors.7 Genomic profiling is accomplished by integration of data from targeted DNA and RNA profiling with up-to-date interpretation ...
... The assay we developed uses next-generation DNA and RNA sequencing and builds on the workflow that has been optimized for genomic profiling of DNA from patients with solid tumors.7 Genomic profiling is accomplished by integration of data from targeted DNA and RNA profiling with up-to-date interpretation ...
Microbial Diversity in Prince Edward County`s Soil Microbiome
... have less microbial diversity than those farther away from the farms. Physiochemical properties of soil can determine the function and structure of microbial communities (Lombard, N., Prestat, E., van Elsas, J. D. and Simonet, P). However, because many types of bacteria require specific environmenta ...
... have less microbial diversity than those farther away from the farms. Physiochemical properties of soil can determine the function and structure of microbial communities (Lombard, N., Prestat, E., van Elsas, J. D. and Simonet, P). However, because many types of bacteria require specific environmenta ...
Mechanistic Comparison of High-Fidelity and Error
... to DNA polymerization, it is first necessary to define fidelity. DNA polymerase fidelity has been measured in different ways, including (a) comparison of kinetic constants for Watson-Crick and mismatched nucleotide incorporation reactions and (b) measurement of misincorporation frequency when compet ...
... to DNA polymerization, it is first necessary to define fidelity. DNA polymerase fidelity has been measured in different ways, including (a) comparison of kinetic constants for Watson-Crick and mismatched nucleotide incorporation reactions and (b) measurement of misincorporation frequency when compet ...
simultaneous detection of colorectal cancer mutations in stool
... stool DNA is a much more patient-friendly option, as it is non-invasive, requires no unpleasant cathartic preparation and allows for off-site collection of samples (6). The most common pathway of CRC development is the chromosomal instability (CIN) pathway, which includes point mutations that occur ...
... stool DNA is a much more patient-friendly option, as it is non-invasive, requires no unpleasant cathartic preparation and allows for off-site collection of samples (6). The most common pathway of CRC development is the chromosomal instability (CIN) pathway, which includes point mutations that occur ...
Educational Items Section Telomeres Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Telomere binding proteins include: I.3.1. TRF1 (telomeric repeat binding factor 1) - Expressed ubiquitously throughout the cell cycle. - Binds to TTAGGG repeat as a homodimer (at tloops) with great specificity. - Functions in cis to inhibit telomerase-dependent ...
... Telomere binding proteins include: I.3.1. TRF1 (telomeric repeat binding factor 1) - Expressed ubiquitously throughout the cell cycle. - Binds to TTAGGG repeat as a homodimer (at tloops) with great specificity. - Functions in cis to inhibit telomerase-dependent ...
Illustrating Python via Bioinformatics Examples
... Life is definitely digital. The genetic code of all living organisms are represented by a long sequence of simple molecules called nucleotides, or bases, which makes up the Deoxyribonucleic acid, better known as DNA. There are only four such nucleotides, and the entire genetic code of a human can be ...
... Life is definitely digital. The genetic code of all living organisms are represented by a long sequence of simple molecules called nucleotides, or bases, which makes up the Deoxyribonucleic acid, better known as DNA. There are only four such nucleotides, and the entire genetic code of a human can be ...
Nucleic acid double helix
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNA_orbit_animated_static_thumb.png?width=300)
In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA, by James Watson.The DNA double helix polymer of nucleic acids, held together by nucleotides which base pair together. In B-DNA, the most common double helical structure, the double helix is right-handed with about 10–10.5 base pairs per turn. This translates into about 20-21 nucleotides per turn. The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. Given the difference in widths of the major groove and minor groove, many proteins which bind to B-DNA do so through the wider major groove.