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B2 revision notes
B2 revision notes

... 1.19 Be able to demonstrate an understanding of the advantages, disadvantages and risks of cloning mammals 1.20 Know that stem cells in the embryo can differentiate into all other types of cells, but that cells lose this ability as the animal matures 1.21 Be able to demonstrate an understanding of t ...
Notes 1
Notes 1

... c. Intercalary Meristem: Intercalary meristem is present at the base of leaf or internodes. They are present on either side of the node. PROPERTIES OF MERISTEMS: Since cells of meristematic tissue are highly active so they have dense cytoplasm. Vacuole is absent in these cells. These cells have high ...
Introduction to the Cardiovascular System
Introduction to the Cardiovascular System

...  Antigens on surface of RBCs  Screened by immune system  Plasma antibodies attack and agglutinate (clump) foreign antigens ...
Anatomy & Physiology Chapter 3 – Part 3
Anatomy & Physiology Chapter 3 – Part 3

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neural control and coordination
neural control and coordination

... There are two types of photoreceptor cells, namely, rods and cones. These cells contain the light-sensitive proteins called the photopigments. The daylight (photopic) vision and colour vision are functions of cones and the twilight (scotopic) vision is the function of the rods. The rods contain a pu ...
14 Stem Cell Differentiation
14 Stem Cell Differentiation

... variety of specialized cell types. Stem cells are classified into three groups, depending on where they are on the pathway toward differentiation. Totipotent stem cells can produce any kind of cell in the body, and have an unlimited ability to selfrenew. The embryonic cells that form during the firs ...
Cells and Systems
Cells and Systems

... the basic unit of life. Their theory accepted the first two tenets of modern cell theory. In 1858, Rudolf Virchow concluded that all cells come from pre-existing cells, thus completing the classical cell theory. The generally accepted parts of modern cell theory include: ...
NOTES- Inv. 2 Supporting Cells.notebook
NOTES- Inv. 2 Supporting Cells.notebook

... ­All the human organ systems interact in order for a human to live and carry out life  functions.  The most important function is servicing cells. ­In a human, the circulatory system pumps blood, which carries resources to each cell  and carries away waste. ­Cells use glucose and oxygen, provided by ...
Biology 11 C
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...  Know statements of the cell theory  Cell organelles (Figure 3 on page 10); know labels and basic functions of major organelles; know differences between plant and animal cells  4 major macromolecules: o Carbohydrates, lipids, proteins, nucleic acids o Know basic structure and functions (i.e. sub ...
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Biology EOCT Practice Questions Part 2

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Chapter 22
Chapter 22

... suspended within an internal body cavity called the coelom. • The coelom of many terrestrial vertebrates is divided into two parts. • Thoracic cavity contains the heart and lungs. • Abdominal cavity contains the stomach, intestines, and liver. ...
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Unit 5 Notes - Flushing Community Schools

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... 1. What is the Cell Theory? What tool is used to observe cells? What units are they measured with? The cell theory is one of the basic principles of biology. The cell theory arose in the mid 1800’s. The cell theory states…. - All living organisms are composed of cells. They may be unicellular or mul ...
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... The information that the nucleus stores is called DNA. DNA is important because it allows that cell to make more cells and also tells it what to do. DNA tells the cell membrane what it can let into the cell and what might be dangerous. It is the DNA that gives the organelles instructions on how to d ...
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No Slide Title

... 1. Types and functions of cells-• For example: Epithelial, CT, Nervous, Muscular 2. Characteristics of the matrix (extracellular material) • Rubbery, stony, or gelatinous 3. Relative amount of space occupied by cells versus matrix • CT vs. muscle and epithelium ...
AQA Knowledge test ANSWERS Unit 2 Biology B2.1_Cells and
AQA Knowledge test ANSWERS Unit 2 Biology B2.1_Cells and

... An allele which controls a characteristic when it is present on only one of the  chromosomes in the pair  5. What is a recessive allele?  An allele which controls the development of characteristics only if the dominant  allele is not present.  6. Which molecule are chromosomes made from and what is  ...
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Feedback Mechanisms and Types of Neurons

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Bio 127 Section 4 Outline
Bio 127 Section 4 Outline

... 1) tissues from this region located in back of embryo surrounding spinal cord. 2) Cells form somites, muscle, and connective tissues of the back C) Intermediate mesoderm 1) forms urogenital system which includes kidneys, gonads, and associated ducts D) Lateral plate mesoderm 1) Farthest away from no ...
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... of regulatory genes that are active in a cell and give it its identity. The similarities between the fingerprints of vasotocin and RFamide-secreting cells in zebrafish and Platynereis are so big that they are difficult to explain by coincidence. Instead they indicate a common evolutionary origin of ...
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Neuronal lineage marker



A Neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells as neurons. It allows detection and identification of cells by using different techniques. A neuronal lineage marker can be either DNA, mRNA or RNA expressed in a cell of interest. It can also be a protein tag, as a partial protein, a protein or a epitope that discriminates between different cell types or different states of a common cell. An ideal marker is specific to a given cell type in normal conditions and/or during injury. Cell markers are very valuable tools for examining the function of cells in normal conditions as well as during disease. The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells.The techniques used for its detection can be immunohistochemistry, immunocytochemistry, methods that utilize transcriptional modulators and site-specific recombinases to label specific neuronal population, in situ hybridization or fluorescence in situ hybridization (FISH). A neuronal lineage marker can be a neuronal antigen that is recognized by an autoantibody for example Hu, which is highly restricted to neuronal nuclei. By immunohistochemistry, anti-Hu stains the nuclei of neurons. To localize mRNA in brain tissue, one can use a fragment of DNA or RNA as a neuronal lineage marker, a hybridization probe that detects the presence of nucleotide sequences that are complementary to the sequence in the probe. This technique is known as in situ hybridization. Its application have been carried out in all different tissues, but particularly useful in neuroscience. Using this technique, it is possible to locate gene expression to specific cell types in specific regions and observe how changes in this distribution occur throughout the development and correlate with the behavioral manipulations.Although immunohistochemistry is the staple methodology for identifying neuronal cell types, since it is relatively low in cost and a wide range of immunohistochemical markers are available to help distinguish the phenotype of cells in the brain, sometimes it is time-consuming to produce a good antibody. Therefore, one of the most convenient methods for the rapid assessment of the expression of a cloned ion channel could be in situ hybridization histochemistry.After cells are isolated from tissue or differentiated from pluripotent precursors, the resulting population needs to be characterized to confirm whether the target population has been obtained. Depending on the goal of a particular study, one can use neural stem cells markers, neural progenitor cell markers, neuron markers or PNS neuronal markers.
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