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Gene Section EIF3C (eukaryotic translation initiation factor 3, subunit C)
Gene Section EIF3C (eukaryotic translation initiation factor 3, subunit C)

... Schwannomin inhibits tumorigenesis through direct interaction with the eukaryotic initiation factor subunit c (eIF3c). Human molecular genetics 2006 ;15 (7):1059-1070. ...
Gene Section PRAME (preferentially expressed antigen in melanoma) Atlas of Genetics and Cytogenetics
Gene Section PRAME (preferentially expressed antigen in melanoma) Atlas of Genetics and Cytogenetics

... identified as a gene encoding a novel cancer-testis antigen that is over expressed in melanoma (Ikeda et al., 1997). Its evolution is consistent with adaptive (positive) selection similar to gene clusters involved in immunity and reproduction, such as the NALP family (Tian et al., 2009). Expression ...
Protein Structure and Bioinformatics
Protein Structure and Bioinformatics

... • What is protein structure? • What are proteins made of? • What forces determines protein structure? • What is protein secondary structure? • What are the primary secondary structures? • How are protein structures determined experimentally? • How can structures be predicted in silico? ...
Buffers
Buffers

... E) None of these peptide will be alpha helical because they all contain lefthanded amino acids. ...
Document
Document

... 1. Standard ProteinProphet output and web interface Each protein group entry contains information on protein name(s), probability, percentage of the sequence covered by assigned peptides, peptide counts, assigned spectra statistics, and links to related groups, if applicable. Within each group one f ...
Polyomaviridae
Polyomaviridae

... • The small T-antigen protein is also able to activate several cellular pathways which stimulate cell proliferation. • Such as the mitogen-activated protein kinase (MAPK) pathway, and the stressactivated protein kinase (SAPK) ...
EVOLUTION
EVOLUTION

... Operon turned on (lactose inactivates repressor) ...
Lecture 1: Overview of bioinformatics
Lecture 1: Overview of bioinformatics

... Suppose you have identified a gene. What is its role in the biochemistry of its organism? Sequence databases can help us in formulating reasonable hypotheses.  Search the database for proteins with similar amino acid sequences in other organisms.  If the functions of the most similar proteins are ...
Recombinant Ebola virus VP40 matrix protein
Recombinant Ebola virus VP40 matrix protein

... and 0.1% Triton-X, at a concentration of 1.602 mg/mL. The theoretical molecular weight of the protein is ~35 kDa. Relevance: Recombinant EBOV VP40 matrix protein provides a means as a control protein for immunoassays and a tool to enhance Filovirus research. SDS-PAGE & Western Blot: Quality control ...
Lab 6
Lab 6

... Laboratory 6 ...
Protein Activity Control
Protein Activity Control

... It is synthesized as a single-chain polypeptide of approx. 150 kDa, subsequently cleaved to form a di-chain molecules, in which a single disulfide bond links the light (50 kDa) and heavy chains (100 kDa) ...
Genetic encoding of the post-translational modification 2
Genetic encoding of the post-translational modification 2

... Critical to the study of PTMs is the ability to produce pure protein samples containing homogeneous compositions of a modified residue. Lysine modifications in particular may offer the ability to generate a designer chromatin9 to study the effects of PTMs either individually or in concert. Modified am ...
Cellular Control revision - Mrs Jones A
Cellular Control revision - Mrs Jones A

... O (Genes are not found in operons in Eukaryotes, so does not apply) O Operons consist of a group of closely linked genes that act together and code for enzymes that control a particular metabolic pathway. An operon consists of at least one structural gene coding for the primary structure of an enzym ...
Proteins
Proteins

... Hydrogen bonds (like magnets) and sulfur-sulfur bonds (like superglue) help make it stronger ...
Protein Purification and Analysis Day 4
Protein Purification and Analysis Day 4

... Proteins have a specific conformation, the spatial arrangement of atoms. Proteins in their functional, folded conformations are in their native state. Native conformations are not very stable. ...
Kretsu Anna PFUR
Kretsu Anna PFUR

... These systems allow to investigate the influence of exogenous factors on their functioning (ionic conditions, pH, inhibitors and activators, etc.). In cell-free system we can replace individual components or directly affect them in an isolated state and then by the reaction system to know their func ...
030607
030607

... • Not even close ...
The exploitation of chromosome recombination between Lolium and
The exploitation of chromosome recombination between Lolium and

... in this way. This is why higher organisms – fungi, plants and animals – are also used in such processes. In these systems, proteins are subjected to all the modifications needed for them to function properly. Complete proteins can be obtained quickest though the use of mammalian cell cultures. These ...
print last name first name
print last name first name

... d. What effect does increasing the enzyme concentration have on Keq? ____________________ e. If Keq>1, is the reaction endergonic or exergonic? _______________________________ 4. Having finished his Bio 1 lecture, Dr. Green is running to Rock Bottom when suddenly his muscles run out of oxygen. (a)__ ...
Proteins POSTER ppt
Proteins POSTER ppt

... structural features. Developing better methods to generate highly representative and compact signatures is a crucial step in designing scalable and accurate data mining systems for proteins. We propose LFM-Pro (Local Feature Mining in Proteins) as a framework for automatically discovering family spe ...
2. Purification of WDR77
2. Purification of WDR77

... acid dipeptide (WD). WD repeat generally contain 7 repeats that are arranged in a circular manner forming a β propeller structure (Figure 1). This structure enables WD repeat domains to act as a scaffold to accommodate the interaction and assembly with a diverse array of proteins and their structure ...
Current Approaches to Protein Purification Richard
Current Approaches to Protein Purification Richard

... You are given a shoe box full of an assortment of small objects including: Ping Pong balls Sugar cubes Paper clips 1/2” brass screws Iron filings 1. List the properties of each of these components that might help you fractionate them. 2. Devise the most efficient method you can for getting pure pape ...
MPS1 Antibody / Metallopan-stimulin 1 (R32561)
MPS1 Antibody / Metallopan-stimulin 1 (R32561)

... ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal pro ...
How to classify proteins on basis of structure?
How to classify proteins on basis of structure?

... If 100 psec (10-10 sec) were required to convert from a conformation to another one, a random search of all conformations would require 5 x 1047 x 10-10 sec ≒ 1.6 x 1030 years. However, folding of proteins takes place in msec to sec order. Therefore, proteins fold not via a random search but a more ...
EJU Syllabus for Biology for printing
EJU Syllabus for Biology for printing

... (3) Photosynthesis [Example] photosystem I, photosystem II, Calvin-Benson cycle, electron transport system (4) Bacterial photosynthesis and chemosynthesis (5) Nitrogen assimilation ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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