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Maize Metabolic Network Construction and Transcriptome Analysis
Maize Metabolic Network Construction and Transcriptome Analysis

... channels. We created MaizeCyc version 2.0.2 based on the protein coding genes identified in the B73 maize variety reference genome sequence assembly named B73 RefGen_v2 (Schnable et al., 2009). This was done by using an integrated approach that involved electronic and systematic annotation of metabo ...
Friesland Foods Normal
Friesland Foods Normal

... micro-organisms applied in these products degrade cysteine and methionine, resulting in the production of flavour components such as methanethiol, dimethyl sulphide (DMS), dimethyl disulphide (DMDS) and dimethyl trisulphide (DMTS). Insight in the regulatory signals and pathways that control the corr ...
Human Metabolism Compared to Other Species
Human Metabolism Compared to Other Species

... We are more closely related to eukaryotes than bacteria. ...
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics
Cancer Prone Disease Section Oculocutaneous Albinism Atlas of Genetics and Cytogenetics

... similar to exons IV and V of TYR gene. It is hypothesized that duplication of TYR exons IV and V regions followed by 11q:11p translocation has given rise to the TYRL segment. Protein Description TYR (monophenol monoxygenase EC 1.14.18.1) encodes a ~80 kDa glycoprotein (Accession No: NP_000363.1) com ...
Yeobeyondgenome_final
Yeobeyondgenome_final

... Applications of ISREs: (2) predicting RNA binding sites ...
P N RANGARAJAN lecture 21
P N RANGARAJAN lecture 21

... -DNA-binding domain (DBD) 5’ to ligand binding domain -A nonconserved hypervariable region, which may contribute to transcriptional activity of receptor ...
Hybrid tryptophan aporepressor containing ligand binding sites
Hybrid tryptophan aporepressor containing ligand binding sites

... anti-CR2 monoclonal antibody OKB7 is T cellsdepend ent, and requires T cell-derived B cell growth factors only short stretches of amino acids, e.g. at least three 35 (Cooper et al., supra (1988)). It has been shown that amino acids, on the surfaces of mammalian proteins are optimal cell cycle progre ...
PowerPoint Presentation - Inducible Genes
PowerPoint Presentation - Inducible Genes

... available, a small amount of it is taken up and converted to allolactose by β-galactosidase. The allolactose binds to the repressor, causing it to fall off the operator site. ...
BRED: Bacteriophage Recombineering with
BRED: Bacteriophage Recombineering with

... mycobacteriophages that we have termed BRED: Bacteriophage recombineering with electroporated DNA. This method utilizes recombineering-proficient strains of Mycobacterium smegmatis that have elevated recombination frequencies due to the expression of phage-derived proteins. It is described in furthe ...
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... 1. First Law of Thermodynamics Energy can be changed from one form into another, but cannot be created nor destroyed. Energy can be stored in various forms then changed into other forms. For example, energy in glucose is oxidized to change the energy stored in chemical bonds into mechanical energy. ...
Chpt3_Isolating_analyzing_genes.doc
Chpt3_Isolating_analyzing_genes.doc

... Plasmids are autonomously replicating circular DNA molecules found in bacteria. They have their own origin of replication, and they replicate independently of the origins on the "host" chromosome. Replication is usually dependent on host functions, such as DNA polymerases, but regulation of plasmid ...
Characterization of Rice Group 3 LEA Genes in Developmental
Characterization of Rice Group 3 LEA Genes in Developmental

... hydrophilicity and a high proportion of glycine or small amino acids (alanine, serine, and threonine), but they lack or have low content of tryptophan and cysteine residues (Stacy and Aalen 1998). LEA proteins are also characterized by their thermal stability, nonglobular structure, and low complexi ...
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Evaluation and Comparison of the GUS, LUC and GFP Reporter

... Green fluorescent protein (GFP) is a 27-kD protein from the jellyfish Aequorea victoria that due to its unique structure, shows a bright green fluorescence when folded correctly and illuminated with UV or blue light (Fig. 1 D; Chalfie et al., 1994). For effective expression in plants, the GFP coding ...
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BL21(DE3) Competent cells E. coli Chemically-competent

... Your protein might be unstable, try different induction conditions (lower temperature, StabyTMSwitch medium, longer induction time,…). Your plasmid could be unstable due to the gene encoding the protein of interest. Check the literature (if data are available). Use a plasmid stabilization system (St ...
Nitrogen-fixing symbiosis
Nitrogen-fixing symbiosis

... Poland, Argentina, Uruguay and Brazil. If you live in those countries, or any nearby, chances are good that there is a laboratory research group that is working on some aspect of this. I ...
Simplex sigillum veri New approaches to the analysis of
Simplex sigillum veri New approaches to the analysis of

... • post-genomic technologies produces “lists” • neighborhood relationships yield “evidence networks (graphs) • lists + graphs = biological insights • GiGA graph analysis highlights and connects relevant areas in the “evidence network” ...
Lesson 2 - The George S. Wise Faculty of Life Sciences
Lesson 2 - The George S. Wise Faculty of Life Sciences

... sequence - the sequence with which we are searching  Hit – a sequence found in the database, suspected as homologous ...
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Lecture 2: Mutation and its effect

... N terminus of a protein contains a free amino group. C terminus of protein contains a free carboxylic acid group. ...
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Agrobacterium tumefaciens T DNA mediated transformation of
Agrobacterium tumefaciens T DNA mediated transformation of

... In the association between E. festucae and perennial ryegrass the endophyte grows systemically in the intercellular spaces of vegetative and reproductive tissues as infrequently branched hyphae parallel to the axis of the leaf (Tan et al. 2001; Christensen et al. 2002). Growth of the hyphae is stric ...
Lab Stn #1  Unit 5 DNA to Protein 
Lab Stn #1 Unit 5 DNA to Protein 

... In transcription, DNA bases are paired with complementary RNA bases according to the base-pairing rules: C-G and A-U. The enzyme RNA polymerase catalyzes this reaction using ATP. The number of DNA base-pairs is determined by the number of amino acids in the resulting polypeptide. The length of DNA c ...
DNA Before Proteins? Recent Discoveries in
DNA Before Proteins? Recent Discoveries in

... (1971) model where every mutation from the master sequence is considered deleterious (Kun et al., 2005; Takeuchi et al., 2005). The possibility remains, however, that the riboorganism genome had reached the maximum allowed by the fidelity of RNA polymerase ribozymes (Johnston et al., 2001; Zaher and ...
Gene therapy: Current status and future perspectives
Gene therapy: Current status and future perspectives

... DNA vaccination. Clinical trials to inject naked DNA plasmids have been performed successfully, however, the expression has been very low in comparison to other methods of transfection(19,20). Electroporation- It is temporary destabilization of the cell membrane targeted tissue by insertion of a pai ...
I. DNA, Chromosomes, Chromatin, and Genes II. DNA
I. DNA, Chromosomes, Chromatin, and Genes II. DNA

... 1. RNA polymerase (enzyme) attaches at a specific location on DNA 2. The enzyme then causes the DNA strands to separate from one another and allow one of the DNA strands to be decoded 3. mRNA nucleotides are floating around in the nucleus find their complement on the DNA stand and bond together. Thi ...
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Endogenous retrovirus



Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.
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