Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell
... Cultures were fed with a defined mineral medium that limited growth by glucose or ethanol. The medium composition was based on that used by Verduyn et al. (1992). The concentration of substrate carbon in the reservoir medium was 250 mmol C l21 for ethanol-limited cultures and 830 mmol C l21 for gluc ...
... Cultures were fed with a defined mineral medium that limited growth by glucose or ethanol. The medium composition was based on that used by Verduyn et al. (1992). The concentration of substrate carbon in the reservoir medium was 250 mmol C l21 for ethanol-limited cultures and 830 mmol C l21 for gluc ...
Bio 30 Complete Outcome Checklist
... technology can be used to transform cells by inserting new DNA or genes into their genome. _____ I can explain how the sequence of nitrogen bases in DNA can give evidence for the relationships among organisms of different species. _____ I understand that very small amounts of DNA are found in chloro ...
... technology can be used to transform cells by inserting new DNA or genes into their genome. _____ I can explain how the sequence of nitrogen bases in DNA can give evidence for the relationships among organisms of different species. _____ I understand that very small amounts of DNA are found in chloro ...
A cucurbit androecy gene reveals how unisexual flowers
... David A. Campbell,11 Nancy R. Sturm,11 Steve Dagenais-Bellefeuille,12 David Morse12 Dinoflagellates are important components of marine ecosystems and essential coral symbionts, yet little is known about their genomes. We report here on the analysis of a high-quality assembly from the 1180-megabase g ...
... David A. Campbell,11 Nancy R. Sturm,11 Steve Dagenais-Bellefeuille,12 David Morse12 Dinoflagellates are important components of marine ecosystems and essential coral symbionts, yet little is known about their genomes. We report here on the analysis of a high-quality assembly from the 1180-megabase g ...
Gene Expression Profiling During Asexual Development of the Late
... Several approaches appraised the technical quality of the array and analysis pipeline. To evaluate how many genes were detectable, hybridizations were performed using RNA from 89 tissues. These included the developmental stages addressed by this article (germinated and ungerminated asexual spores, p ...
... Several approaches appraised the technical quality of the array and analysis pipeline. To evaluate how many genes were detectable, hybridizations were performed using RNA from 89 tissues. These included the developmental stages addressed by this article (germinated and ungerminated asexual spores, p ...
Paper
... mapping sequencing reads to a reference genome, sequencing errors, and genetic variation. i et al. (1) sequenced cDNA from lymphoblastoid cell lines derived from 27 individuals whose genomes have been sequenced at low coverage (2) and identified 10,210 sites of mismatches between an individual’s mRN ...
... mapping sequencing reads to a reference genome, sequencing errors, and genetic variation. i et al. (1) sequenced cDNA from lymphoblastoid cell lines derived from 27 individuals whose genomes have been sequenced at low coverage (2) and identified 10,210 sites of mismatches between an individual’s mRN ...
Noise in eukaryotic gene expression
... To explore the effects of transcriptional variation and control on the level of noise in eukaryotic gene expression, we used both native and artificial modes of transcriptional regulation in the yeast GAL1 promoter (Fig. 1a). In its natural context, the GAL1 promoter is activated in response to gala ...
... To explore the effects of transcriptional variation and control on the level of noise in eukaryotic gene expression, we used both native and artificial modes of transcriptional regulation in the yeast GAL1 promoter (Fig. 1a). In its natural context, the GAL1 promoter is activated in response to gala ...
Extralenticular expression of Xenopus laevis alpha-, beta
... which correlates well with the length of the corresponding mRNA, determined by Northern blot analEmbryos ysis (data not shown). It codes for a protein of 196 Xenopus laevis embryos were obtained from the Depart- amino acids. Sequence comparison revealed that ment of Zoology of the University of Nijm ...
... which correlates well with the length of the corresponding mRNA, determined by Northern blot analEmbryos ysis (data not shown). It codes for a protein of 196 Xenopus laevis embryos were obtained from the Depart- amino acids. Sequence comparison revealed that ment of Zoology of the University of Nijm ...
Back-translation for discovering distant protein homologies
... We designed and implemented an efficient method for aligning putative coding DNA sequences, which builds expressive alignments between hypothetical nucleotide sequences that can provide some information about the common ancestral sequence, if such a sequence exists. We perform the analysis on memory ...
... We designed and implemented an efficient method for aligning putative coding DNA sequences, which builds expressive alignments between hypothetical nucleotide sequences that can provide some information about the common ancestral sequence, if such a sequence exists. We perform the analysis on memory ...
Targeting the GAA-Repeat Region with Oligonucleotides for the
... screens identified two chromatin modifying complexes as being important in establishing and/or maintaining repeat expansion-induced transcriptional repression at the FXN locus. We identified a novel putative non-coding RNA (ncRNA) potentially responsible for directing the localized epigenetic silenc ...
... screens identified two chromatin modifying complexes as being important in establishing and/or maintaining repeat expansion-induced transcriptional repression at the FXN locus. We identified a novel putative non-coding RNA (ncRNA) potentially responsible for directing the localized epigenetic silenc ...
Editing the Mushroom
... to replicate a mutation that naturally occurs in Angus beef cattle, which do not grow horns. Ag scientists tout this application of gene editing as a more humane form of farming because it spares male Holstein cows from a gruesome procedure during which dairy farmers physically gouge out and then ca ...
... to replicate a mutation that naturally occurs in Angus beef cattle, which do not grow horns. Ag scientists tout this application of gene editing as a more humane form of farming because it spares male Holstein cows from a gruesome procedure during which dairy farmers physically gouge out and then ca ...
Nomenclature of the ARID family of DNA
... ARID1A and ARID1B are 80% identical within the ARID and approximately 50% identical across their full-length amino acid sequences, although ARID1A has additional glutamine-rich regions and several LXXLL motifs (presumptive nuclear hormone receptor-binding sites) that are not precisely conserved in A ...
... ARID1A and ARID1B are 80% identical within the ARID and approximately 50% identical across their full-length amino acid sequences, although ARID1A has additional glutamine-rich regions and several LXXLL motifs (presumptive nuclear hormone receptor-binding sites) that are not precisely conserved in A ...
PLASMA PROTEINS Plasma is non-cellular portion of blood. The
... carrying phosphate is shown on the left hand side and 3' end carrying unreacted hydroxyl is shown on the right hand side . Primary structures of DNA and RNA exist in single stranded DNA and RNA organisms. Since polynucleotide consists of various bases, sugars and phosphates writing a segment of poly ...
... carrying phosphate is shown on the left hand side and 3' end carrying unreacted hydroxyl is shown on the right hand side . Primary structures of DNA and RNA exist in single stranded DNA and RNA organisms. Since polynucleotide consists of various bases, sugars and phosphates writing a segment of poly ...
Differential expression of arid5b isoforms in Xenopus
... (Fig.S2). Using InterProSCan software and performing an extensive analysis of the litterature on Arid5 family members, we could not find any known domain in this region, precluding to identify any functional difference ...
... (Fig.S2). Using InterProSCan software and performing an extensive analysis of the litterature on Arid5 family members, we could not find any known domain in this region, precluding to identify any functional difference ...
Regulation of gene expression: Eukaryotic
... Termination of Transcription in Prokaryotes • A specific nucleotide sequence acts as a termination signal, about 40 base pairs in length • Sometimes a special protein called termination factor, rho is required for termination • At termination, RNA dissociates from DNA and enzyme (RNA polymerase) fa ...
... Termination of Transcription in Prokaryotes • A specific nucleotide sequence acts as a termination signal, about 40 base pairs in length • Sometimes a special protein called termination factor, rho is required for termination • At termination, RNA dissociates from DNA and enzyme (RNA polymerase) fa ...
Isolating and Analyzing Genes
... Recombinant DNA, Polymerase Chain Reaction and Applications to Eukaryotic Gene Structure and Function The first two chapters covered many important aspects of genes, such as how they function in inheritance, how they code for protein (in general terms) and their chemical nature. All this was learned ...
... Recombinant DNA, Polymerase Chain Reaction and Applications to Eukaryotic Gene Structure and Function The first two chapters covered many important aspects of genes, such as how they function in inheritance, how they code for protein (in general terms) and their chemical nature. All this was learned ...
Silver PA, Brent R, Ptashne M. DNA binding is not
... Certain proteins are found only in the cell nucleus. Following their synthesis in the cytoplasm, these proteins move into the nucleus in a way we do not understand. One possibility is that proteins diffuse into the nucleus through the nuclear pores and are retained there by binding to DNA or chromat ...
... Certain proteins are found only in the cell nucleus. Following their synthesis in the cytoplasm, these proteins move into the nucleus in a way we do not understand. One possibility is that proteins diffuse into the nucleus through the nuclear pores and are retained there by binding to DNA or chromat ...
Human/Mouse/Rat PP2A Catalytic Subunit Antibody
... Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the Cterminus at Y307 of the catalytic subunit decrea ...
... Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the Cterminus at Y307 of the catalytic subunit decrea ...
Motif Finding
... • The hidden sequence is of length 8 • The pattern is not exactly the same in each array because random point mutations may occur in the sequences ...
... • The hidden sequence is of length 8 • The pattern is not exactly the same in each array because random point mutations may occur in the sequences ...
Analysis of the LacI family of repressor proteins in non
... It is a cogent depiction of how a set of 'structural' genes may be coordinately transcribed in response to environmental conditions and regulates metabolic events in the cell (Lewis, 2005). Binding of the lacO operator region by the LacI repressor protein in E. coli is well studied. Blast analysis o ...
... It is a cogent depiction of how a set of 'structural' genes may be coordinately transcribed in response to environmental conditions and regulates metabolic events in the cell (Lewis, 2005). Binding of the lacO operator region by the LacI repressor protein in E. coli is well studied. Blast analysis o ...
Molecular Evolution of Nitrate Reductase Genes
... Also, two large insertions (14–22 amino acids) were observed in the MoCo domains of fungal NR genes. No gap was observed in the heme domain of any NR gene, which may reflect the high functional constraint on this region. Based on the alternative peptide symbol comparison table StructGappep.cmp in th ...
... Also, two large insertions (14–22 amino acids) were observed in the MoCo domains of fungal NR genes. No gap was observed in the heme domain of any NR gene, which may reflect the high functional constraint on this region. Based on the alternative peptide symbol comparison table StructGappep.cmp in th ...
MAST CELL DISEASE & Ig E
... modified cardiovascular burden in patients with rheumatoid arthritis. Most show an increase in HDL and total cholesterol, but the long-term effects of TNF-α blockade on lipid patterns are still unclear. The mechanisms of action of such treatment have not been fully explored. ...
... modified cardiovascular burden in patients with rheumatoid arthritis. Most show an increase in HDL and total cholesterol, but the long-term effects of TNF-α blockade on lipid patterns are still unclear. The mechanisms of action of such treatment have not been fully explored. ...
Figure 2: Construction of a gene deletion using BRED.
... mycobacteriophages that we have termed BRED: Bacteriophage recombineering with electroporated DNA. This method utilizes recombineering-proficient strains of Mycobacterium smegmatis that have elevated recombination frequencies due to the expression of phage-derived proteins. It is described in furthe ...
... mycobacteriophages that we have termed BRED: Bacteriophage recombineering with electroporated DNA. This method utilizes recombineering-proficient strains of Mycobacterium smegmatis that have elevated recombination frequencies due to the expression of phage-derived proteins. It is described in furthe ...
Introduction to Protein Labeling
... • First step of the process involves the insertion of the DNA coding region of the protein of interest into a plasmid. plasmid - small, circular pieces of DNA that are found in E. coli and many other bacteria generally remain separate from the bacterial chromosome carry genes that can be expres ...
... • First step of the process involves the insertion of the DNA coding region of the protein of interest into a plasmid. plasmid - small, circular pieces of DNA that are found in E. coli and many other bacteria generally remain separate from the bacterial chromosome carry genes that can be expres ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.