Human Biotechnology
... Human Biotechnology • we are not sure if we want one, or if we do, what traits we would want ...
... Human Biotechnology • we are not sure if we want one, or if we do, what traits we would want ...
PS401 – Lec 10
... SAGEmap – A way to look at SAGE data inlcuding two-way mapping between SAGE tag and UniGene. Gene Expression Omnibus (GEO) – Data repository and retrieval system for expression data from all sources. OMIM – Catalog of human genes and genetic disorders including phenotypes and polymorphism inform ...
... SAGEmap – A way to look at SAGE data inlcuding two-way mapping between SAGE tag and UniGene. Gene Expression Omnibus (GEO) – Data repository and retrieval system for expression data from all sources. OMIM – Catalog of human genes and genetic disorders including phenotypes and polymorphism inform ...
슬라이드 1
... ▶ WRKY (pronounced‘worky’) proteins, a large family of transcriptional regulators that has to date only been found in plants. ▶ The WRKY domain is defined by the conserved amino acidsequence WRKYGQK at its N-terminal end, together with a novel zinc-finger-like motif ▶ Many WRKY proteins having a reg ...
... ▶ WRKY (pronounced‘worky’) proteins, a large family of transcriptional regulators that has to date only been found in plants. ▶ The WRKY domain is defined by the conserved amino acidsequence WRKYGQK at its N-terminal end, together with a novel zinc-finger-like motif ▶ Many WRKY proteins having a reg ...
Viruses
... • non-cellular; do not respire, grow/ develop, respond to stimuli • Parasitic on living cells - nucleic material in viruses give information for production of new viruses • viruses are spread from infected cells by direct contact or indirectly through air, water, food, fecal material ...
... • non-cellular; do not respire, grow/ develop, respond to stimuli • Parasitic on living cells - nucleic material in viruses give information for production of new viruses • viruses are spread from infected cells by direct contact or indirectly through air, water, food, fecal material ...
Last5
... • Sanger-Schachter theory of emotion: emotion is a function both of cognition (thought) and physiological state • these lead to the bonds we form: we know that oxytocin (involved in maternal bonding/imprinting with offspring) and vasopressin are two key players ...
... • Sanger-Schachter theory of emotion: emotion is a function both of cognition (thought) and physiological state • these lead to the bonds we form: we know that oxytocin (involved in maternal bonding/imprinting with offspring) and vasopressin are two key players ...
bacteriophage
... The phage fX174 is an icosahedral phage that contains a circular singlestranded DNA molecule of 5386 nucleotides. It codes for 11 proteins, each of which has been identified. Adding together the size of all those proteins comes to 2330 amino acids, which1 would require 6990 nucleotides (3 2330) – su ...
... The phage fX174 is an icosahedral phage that contains a circular singlestranded DNA molecule of 5386 nucleotides. It codes for 11 proteins, each of which has been identified. Adding together the size of all those proteins comes to 2330 amino acids, which1 would require 6990 nucleotides (3 2330) – su ...
Cloning and selection
... When do the cutting and sticking of plasmid and foreign DNA there are several possible outcomes 1. Successful sticking of the plasmid and foreign DNA 2. Recircularization of plasmid without the foreign DNA 3. Circulization of plasmid with other plasmids or several inserts to make huge circular mol ...
... When do the cutting and sticking of plasmid and foreign DNA there are several possible outcomes 1. Successful sticking of the plasmid and foreign DNA 2. Recircularization of plasmid without the foreign DNA 3. Circulization of plasmid with other plasmids or several inserts to make huge circular mol ...
Specialties: Microbial Diversity and Bacterial Membrane Proteins
... permease subsequently leads to a conformational change and releases them into the cytoplasm. Hence, permeases must display a high degree of specificity in substrate recognition in order to discriminate between different molecules presented to the cell. However, the precise mechanisms of these import ...
... permease subsequently leads to a conformational change and releases them into the cytoplasm. Hence, permeases must display a high degree of specificity in substrate recognition in order to discriminate between different molecules presented to the cell. However, the precise mechanisms of these import ...
Mutations
... Mutant alleles are rare variants – identified through clinically significant disorder (disease-causing variants) More mutant alleles at same locus (each capable of producing an abnormal phenotype)= allelic heterogeneity But some of rare variants appear to have no deleterious effect, i.e. there is „ ...
... Mutant alleles are rare variants – identified through clinically significant disorder (disease-causing variants) More mutant alleles at same locus (each capable of producing an abnormal phenotype)= allelic heterogeneity But some of rare variants appear to have no deleterious effect, i.e. there is „ ...
The four types of nucleotides in DNA are Adenine, Thymine
... Carrying information for protein synthesis to the cytoplasm Storing genetic information while the cell is replicating Carrying genetic information between cells Indicating the transcription start site ...
... Carrying information for protein synthesis to the cytoplasm Storing genetic information while the cell is replicating Carrying genetic information between cells Indicating the transcription start site ...
Gene tagging (Dr. H S Parmar)
... Genome wide insertional mutagenesis in yeast: Use of endogenous and heterologous transposons -One strategy is use of Ty element as an insertional mutagen………..libraries of mutants generated carrying Ty……used as “genetic foot print”. As it is endogenous……….therefore, modified element with a unique DN ...
... Genome wide insertional mutagenesis in yeast: Use of endogenous and heterologous transposons -One strategy is use of Ty element as an insertional mutagen………..libraries of mutants generated carrying Ty……used as “genetic foot print”. As it is endogenous……….therefore, modified element with a unique DN ...
DNA Technology and Genomics
... Human DNA and plasmid are treated with the same restriction enzyme to produce identical sticky ends DNAs are mixed together and the enzyme DNA ligase used to bond the sticky ends Recombinant plasmid is introduced into a bacterial cell by simply adding the DNA to a bacterial culture where some bacter ...
... Human DNA and plasmid are treated with the same restriction enzyme to produce identical sticky ends DNAs are mixed together and the enzyme DNA ligase used to bond the sticky ends Recombinant plasmid is introduced into a bacterial cell by simply adding the DNA to a bacterial culture where some bacter ...
Cellular Control revision - Mrs Jones A
... the repressor protein •this now cannot bind to the DNA •so RNA polymerase can bind to the promoter •so the gene is transcribed into mRNA •and the enzyme is produced to break down the lactose as a food source i.e. lactose is the signal to switch on the gene ...
... the repressor protein •this now cannot bind to the DNA •so RNA polymerase can bind to the promoter •so the gene is transcribed into mRNA •and the enzyme is produced to break down the lactose as a food source i.e. lactose is the signal to switch on the gene ...
Nucleic Acid Therapeutics
... 13 1999, Gelsinger was injected with adenoviruses carrying a corrected gene in the hope that it would manufacture the needed enzyme. He died four days later, apparently having suffered a massive immune response triggered by the use of the viral vector used to transport the gene into his cells. This ...
... 13 1999, Gelsinger was injected with adenoviruses carrying a corrected gene in the hope that it would manufacture the needed enzyme. He died four days later, apparently having suffered a massive immune response triggered by the use of the viral vector used to transport the gene into his cells. This ...
Gene Section chromosome 18-like 1) Atlas of Genetics and Cytogenetics
... Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903 ...
... Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903 ...
Pairwise Alignments Part 1
... Pairwise GLOBAL alignment of retinol-binding protein from human (top) and rainbow trout (O. mykiss) ...
... Pairwise GLOBAL alignment of retinol-binding protein from human (top) and rainbow trout (O. mykiss) ...
S1 Text
... As might be anticipated from the observation that GATA binding sites are GCpoor, we found that genes previously noted to have two or more potential GATA binding sites [16] are preferentially located in the long GC-poor tracts we delimited in this study. Shorter GC-poor stretches, identified by runn ...
... As might be anticipated from the observation that GATA binding sites are GCpoor, we found that genes previously noted to have two or more potential GATA binding sites [16] are preferentially located in the long GC-poor tracts we delimited in this study. Shorter GC-poor stretches, identified by runn ...
Human Genetics
... AAs to ribosomes. The AA’s join in cytoplasm to form proteins. 20 types. Loop structure – Ribosomal RNA: (rRNA) Joins with proteins made in cytoplasm to form the subunits of ribosomes. Linear ...
... AAs to ribosomes. The AA’s join in cytoplasm to form proteins. 20 types. Loop structure – Ribosomal RNA: (rRNA) Joins with proteins made in cytoplasm to form the subunits of ribosomes. Linear ...
Macro-Microarray
... 2. Label your microarray with the name of the gene that corresponds to each screw. 3. Look at the descriptions of the genes represented in your microarray. Based on what you know about cells and cancer, predict what genes you think might be expressed more or less in a patient who has cancer. 4. Get ...
... 2. Label your microarray with the name of the gene that corresponds to each screw. 3. Look at the descriptions of the genes represented in your microarray. Based on what you know about cells and cancer, predict what genes you think might be expressed more or less in a patient who has cancer. 4. Get ...
Gene Regulation
... do not alter DNA sequence, they may be passed to future generations of cells • The inheritance of traits transmitted by mechanisms not directly involving the nucleotide sequence is called ...
... do not alter DNA sequence, they may be passed to future generations of cells • The inheritance of traits transmitted by mechanisms not directly involving the nucleotide sequence is called ...
BTEC Bowl Questions
... E. (A), (B) and (C) are all correct. (p. 174) Hans Cooper: Q: Enucleation consists of: A: A. preparing an egg for cloning. B. gently suctioning the DNA out of the nucleus C. recombining the DNA from one nucleus with another nucleus. D. both (A) and (B) are correct. (p. 177) E. (A), (B) and (C) are a ...
... E. (A), (B) and (C) are all correct. (p. 174) Hans Cooper: Q: Enucleation consists of: A: A. preparing an egg for cloning. B. gently suctioning the DNA out of the nucleus C. recombining the DNA from one nucleus with another nucleus. D. both (A) and (B) are correct. (p. 177) E. (A), (B) and (C) are a ...
File
... for replication. Before a virus can replicate, it must first infect a living cell. In bacterial viruses, the cycle of viral infection, replication, and cell destruction is called the lytic cycle. After the viral genes have entered the cell, they use the host cell to replicate viral genes and to make ...
... for replication. Before a virus can replicate, it must first infect a living cell. In bacterial viruses, the cycle of viral infection, replication, and cell destruction is called the lytic cycle. After the viral genes have entered the cell, they use the host cell to replicate viral genes and to make ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.