Poster
... derived from viruses are used by Ago-2 to slice virus mRNA, protecting the cells from infection. In the miRNA pathway, Ago-2 utilizes naturally occurring miRNA to slice cellular mRNAs to control protein production. Ago-2 works by binding small (~22 nucleotide) regulatory RNAs (siRNA and miRNA) that ...
... derived from viruses are used by Ago-2 to slice virus mRNA, protecting the cells from infection. In the miRNA pathway, Ago-2 utilizes naturally occurring miRNA to slice cellular mRNAs to control protein production. Ago-2 works by binding small (~22 nucleotide) regulatory RNAs (siRNA and miRNA) that ...
Protein Synthesis and Degradation
... All of the 16S, 23S and 5S rRNA chains, the A-, P- and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with protein ...
... All of the 16S, 23S and 5S rRNA chains, the A-, P- and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with protein ...
Protein thermodynamics: Are native proteins
... greater than M smax, which implies that fibril structure must be the lowest freeenergy structure. Thus it is surmised that the functionally competent states of many proteins must be metastable with respect to the fibril. The analysis also suggests that due to topological frustration longer proteins ...
... greater than M smax, which implies that fibril structure must be the lowest freeenergy structure. Thus it is surmised that the functionally competent states of many proteins must be metastable with respect to the fibril. The analysis also suggests that due to topological frustration longer proteins ...
Protein Translation
... 1.5% encode proteins < = > 98.5% not protein encoding ~ 31,000 genes encoding 100,000 - 200,000 proteins How are 100,000 to 200,000 proteins produced from 31,000 genes? What is the 98.5% of the human genome that does not encode proteins? ...
... 1.5% encode proteins < = > 98.5% not protein encoding ~ 31,000 genes encoding 100,000 - 200,000 proteins How are 100,000 to 200,000 proteins produced from 31,000 genes? What is the 98.5% of the human genome that does not encode proteins? ...
From Gene to Protein
... • Introns: noncoding sequences that are removed • Exons: coding sequences that are spliced together • Small nuclear ribonucleoproteins (snRNPs): identify and help bring about the splicing process • Spliceosome: catalyzes splicing reactions ...
... • Introns: noncoding sequences that are removed • Exons: coding sequences that are spliced together • Small nuclear ribonucleoproteins (snRNPs): identify and help bring about the splicing process • Spliceosome: catalyzes splicing reactions ...
The HicAB cassette, a putative novel, RNA-targeting toxin
... twice as many (450 sequences) HicB proteins than HicA family proteins, with the largest number, again, found in C.watsonii (33, Supplementary material). In part, this is due to the poor detection of the small hicA genes during genome sequence annotation. In several cases, we identified a hicA-like ...
... twice as many (450 sequences) HicB proteins than HicA family proteins, with the largest number, again, found in C.watsonii (33, Supplementary material). In part, this is due to the poor detection of the small hicA genes during genome sequence annotation. In several cases, we identified a hicA-like ...
Lecture 8 RNA Secondary Structure Central Dogma
... RNA • In the central dodgma, we talk about mRNA coding for protein. There are also tRNA and rRNA that are also coded for by the DNA. The MicroRNAs (miRNA) are small (22 nucleotides) non-coding RNA gene products that seem to regulate translation • The RNA has features in it sequence that gives it a ...
... RNA • In the central dodgma, we talk about mRNA coding for protein. There are also tRNA and rRNA that are also coded for by the DNA. The MicroRNAs (miRNA) are small (22 nucleotides) non-coding RNA gene products that seem to regulate translation • The RNA has features in it sequence that gives it a ...
Protein Annotation with GO Codes - dollar
... votes', where a 'vote' is given by the number of documents in the proteins's set that was assigned the code. A protein is assigned all codes that the classifier assign to the protein's document set that have a number of 'votes' at least equal to the margin. The margin is the difference between maxim ...
... votes', where a 'vote' is given by the number of documents in the proteins's set that was assigned the code. A protein is assigned all codes that the classifier assign to the protein's document set that have a number of 'votes' at least equal to the margin. The margin is the difference between maxim ...
RIBONUCLEIC ACID (RNA)
... • Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded unto itself, rather than a paired double-strand • Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, A, U, and C to denote t ...
... • Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded unto itself, rather than a paired double-strand • Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, A, U, and C to denote t ...
13.1 RNA
... Ribosomal RNA Protein synthesis occurs on ribosomes, which are made up of two subunits. Both subunits consist of several molecules of ribosomal RNA (rRNA). ...
... Ribosomal RNA Protein synthesis occurs on ribosomes, which are made up of two subunits. Both subunits consist of several molecules of ribosomal RNA (rRNA). ...
16 RNA extraction
... Non coding RNA is more diverse than the coding RNA and comprises transcripts with a number of different functions, all of which are performed by the RNA molecules themselves. In both prokaryotes and eukaryotes the two main types of non-coding RNA are: Ribosomal RNAs (rRNAs), which are the most abund ...
... Non coding RNA is more diverse than the coding RNA and comprises transcripts with a number of different functions, all of which are performed by the RNA molecules themselves. In both prokaryotes and eukaryotes the two main types of non-coding RNA are: Ribosomal RNAs (rRNAs), which are the most abund ...
DNA Damage - Columbus Labs
... activities that promoted the synthesis of that polypeptide. This method of producing polypeptides with specific amino acid sequences has several limitations. First, it seems likely that only relatively short specific polypeptides could have been produced in this manner. Second, it would have been di ...
... activities that promoted the synthesis of that polypeptide. This method of producing polypeptides with specific amino acid sequences has several limitations. First, it seems likely that only relatively short specific polypeptides could have been produced in this manner. Second, it would have been di ...
B-PERfusions
... alternative to sonication for extracting recombinant proteins from the cytoplasm of Escherichia coli cells carrying expression vectors, especially when many proteins are to be processed in parallel. In this article I’ll summarize our extensive experience with B-PER for isolating fusion proteins in w ...
... alternative to sonication for extracting recombinant proteins from the cytoplasm of Escherichia coli cells carrying expression vectors, especially when many proteins are to be processed in parallel. In this article I’ll summarize our extensive experience with B-PER for isolating fusion proteins in w ...
Prok transcription
... Transcription- synthesis of RNA from only one strand of a double stranded DNA helix DNARNA(Protein) Why is RNA an intermediate???? 1. Protect the DNA; limited access; 2. Gives regulatory opportunity (all cells have the same DNA but not the same genes are expressed) 3. In Eukaryotes the DNA i ...
... Transcription- synthesis of RNA from only one strand of a double stranded DNA helix DNARNA(Protein) Why is RNA an intermediate???? 1. Protect the DNA; limited access; 2. Gives regulatory opportunity (all cells have the same DNA but not the same genes are expressed) 3. In Eukaryotes the DNA i ...
New roles for RNA
... Source of variation • What is the homology of protein coding genes between human and mice? ...
... Source of variation • What is the homology of protein coding genes between human and mice? ...
Powerpoint file - revised
... Model of spliceosome-mediated splicing of pre-mRNA •Five snRNPs (U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles) containing 5 snRNAs (U1, U2, U4, U5 and U6 small nuclear RNAs, ranging from 107 to 210 nucleotides) and their associated proteins (6-10 per snRNP) assemble on the pre-mR ...
... Model of spliceosome-mediated splicing of pre-mRNA •Five snRNPs (U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles) containing 5 snRNAs (U1, U2, U4, U5 and U6 small nuclear RNAs, ranging from 107 to 210 nucleotides) and their associated proteins (6-10 per snRNP) assemble on the pre-mR ...
1 Processing of eukaryotic pre-mRNA
... Model of spliceosome-mediated splicing of pre-mRNA •Five snRNPs (U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles) containing 5 snRNAs (U1, U2, U4, U5 and U6 small nuclear RNAs, ranging from 107 to 210 nucleotides) and their associated proteins (6-10 per snRNP) assemble on the pre-mR ...
... Model of spliceosome-mediated splicing of pre-mRNA •Five snRNPs (U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles) containing 5 snRNAs (U1, U2, U4, U5 and U6 small nuclear RNAs, ranging from 107 to 210 nucleotides) and their associated proteins (6-10 per snRNP) assemble on the pre-mR ...
Document
... the nucleus.To the 3’ end an enzyme adds a _________ tail consisting of 50 to 250 _________________ nucleotides. The 5’ cap and poly-A tail seem to 3 important functions: ...
... the nucleus.To the 3’ end an enzyme adds a _________ tail consisting of 50 to 250 _________________ nucleotides. The 5’ cap and poly-A tail seem to 3 important functions: ...
Tertiary Structure
... Many proteins consist of a single polypeptide chain, and are defined as monomeric proteins. others may consist of two or more polypeptide chains that may be structurally identical or totally unrelated. The arrangement of these polypeptide subunits is called the quaternary structure of the protei ...
... Many proteins consist of a single polypeptide chain, and are defined as monomeric proteins. others may consist of two or more polypeptide chains that may be structurally identical or totally unrelated. The arrangement of these polypeptide subunits is called the quaternary structure of the protei ...
Lecture 9 RNA world and emegence of complexity
... Biological stage Some common biopolymers that could have participated in the formation of early life: Proteins: amino acid diversity, catalysis ...
... Biological stage Some common biopolymers that could have participated in the formation of early life: Proteins: amino acid diversity, catalysis ...
Bioknowlodgy worksheet 2.4
... 2.4.U1 Amino acids are linked together by condensation to form polypeptides. AND 2.4.S1 Drawing molecular diagrams to show the formation of a peptide bond. 1. Condensation of amino acids is a polymerisation reaction. A chain of amino acids joined together is called a polypeptide. These building reac ...
... 2.4.U1 Amino acids are linked together by condensation to form polypeptides. AND 2.4.S1 Drawing molecular diagrams to show the formation of a peptide bond. 1. Condensation of amino acids is a polymerisation reaction. A chain of amino acids joined together is called a polypeptide. These building reac ...
Bioc 462a Lecture Notes
... because it was the basis for the development of procedures for obtaining pure proteins. o Studies on pure proteins are essential for understanding the structural and functional properties of proteins. o In column chromatography an absorbent (see below) is placed in a glass tube. o A protein mixture ...
... because it was the basis for the development of procedures for obtaining pure proteins. o Studies on pure proteins are essential for understanding the structural and functional properties of proteins. o In column chromatography an absorbent (see below) is placed in a glass tube. o A protein mixture ...
L22 RNA, QC
... Non coding RNA is more diverse than the coding RNA and comprises transcripts with a number of different functions, all of which are performed by the RNA molecules themselves. In both prokaryotes and eukaryotes the two main types of non-coding RNA are: Ribosomal RNAs (rRNAs), which are the most abund ...
... Non coding RNA is more diverse than the coding RNA and comprises transcripts with a number of different functions, all of which are performed by the RNA molecules themselves. In both prokaryotes and eukaryotes the two main types of non-coding RNA are: Ribosomal RNAs (rRNAs), which are the most abund ...
LSm
In molecular biology, LSm proteins are a family of RNA-binding proteins found in virtually every cellular organism. LSm is a contraction of 'like Sm', because the first identified members of the LSm protein family were the Sm proteins. LSm proteins are defined by a characteristic three-dimensional structure and their assembly into rings of six or seven individual LSm protein molecules, and play a large number of various roles in mRNA processing and regulation.The Sm proteins were first discovered as antigens targeted by so-called Anti-Sm antibodies in a patient with a form of Systemic lupus erythematosus (SLE), a debilitating autoimmune disease. They were named Sm proteins in honor of Stephanie Smith, a patient who suffered from SLE. Other proteins with very similar structures were subsequently discovered and named LSm proteins. New members of the LSm protein family continue to be identified and reported.Proteins with similar structures are grouped into a hierarchy of protein families, superfamilies, and folds. The LSm protein structure is an example of a small beta sheet folded into a short barrel. Individual LSm proteins assemble into a six or seven member doughnut ring (more properly termed a torus), which usually binds to a small RNA molecule to form a ribonucleoprotein complex. The LSm torus assists the RNA molecule to assume and maintain its proper three-dimensional structure. Depending on which LSm proteins and RNA molecule are involved, this ribonucleoprotein complex facilitates a wide variety of RNA processing including degradation, editing, splicing, and regulation.Alternate terms for LSm family are LSm fold and Sm-like fold, and alternate capitalization styles such as lsm, LSM, and Lsm are common and equally acceptable.