The Serum Proteins of the Rat During Development
... relative concentration of the albumin. This suggests that the material previously recognized as fast albumin does not disappear at all but that it becomes merged with the main bulk of the albumin group. If the fast albumin material had disappeared, all remaining components would be expected to show ...
... relative concentration of the albumin. This suggests that the material previously recognized as fast albumin does not disappear at all but that it becomes merged with the main bulk of the albumin group. If the fast albumin material had disappeared, all remaining components would be expected to show ...
Chimeric phosphorylation indicator
... signal pathWay may be tested With CKAR, calcium-sensing ?uorophores and FRET-based translocation assays. Poly nucleotides encoding such CPIs, and kits containing the indi cators and/ or the polynucleotides, are provided. A method of using the chimeric phosphorylation indicators to detect a ...
... signal pathWay may be tested With CKAR, calcium-sensing ?uorophores and FRET-based translocation assays. Poly nucleotides encoding such CPIs, and kits containing the indi cators and/ or the polynucleotides, are provided. A method of using the chimeric phosphorylation indicators to detect a ...
Fal1p Is an Essential DEAD-Box Protein Involved in 40S
... eIF4A is stimulated by eIF4B (45, 57). During translation initiation, these two proteins are thought to remove secondary structures in the 59 untranslated region of mRNAs. Such secondary structures impede 40S ribosome binding and movement (scanning process) (34). Thus, an RNA helicase activity is li ...
... eIF4A is stimulated by eIF4B (45, 57). During translation initiation, these two proteins are thought to remove secondary structures in the 59 untranslated region of mRNAs. Such secondary structures impede 40S ribosome binding and movement (scanning process) (34). Thus, an RNA helicase activity is li ...
translation and protein structure
... charges in the interacting molecules, which are then attracted to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is als ...
... charges in the interacting molecules, which are then attracted to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is als ...
1 Nucleoli: Composition, Function and Dynamics
... In contrast, characterization of the proteome of plant nucleoli lags significantly behind. In an initial proteomic analysis of purified Arabidopsis nucleoli 217 proteins were identified. In addition to the expected ribosomal and nucleolar proteins, a range of nonribosomal and non-nucleolar proteins ...
... In contrast, characterization of the proteome of plant nucleoli lags significantly behind. In an initial proteomic analysis of purified Arabidopsis nucleoli 217 proteins were identified. In addition to the expected ribosomal and nucleolar proteins, a range of nonribosomal and non-nucleolar proteins ...
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON
... provided by the manufacturer. To account for experimental variations, several gels from independent extractions were prepared. The gel spot pattern of 2-DE gels resulting from the analysis of prefractionated proteins of the P. aeruginosa planktonic, SIP and biofilm populations were summarised in a c ...
... provided by the manufacturer. To account for experimental variations, several gels from independent extractions were prepared. The gel spot pattern of 2-DE gels resulting from the analysis of prefractionated proteins of the P. aeruginosa planktonic, SIP and biofilm populations were summarised in a c ...
Flavors of Protein Disorder
... ago7, and that some proteins don’t fold due to an atypical amino acid composition was suggested more than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "intrinsically unstructured"1, and “intrinsically disordered”2. The failure to self-fold into specific 3-D ...
... ago7, and that some proteins don’t fold due to an atypical amino acid composition was suggested more than 20 years ago8, 9. More recently, such proteins have been called "natively unfolded"10, "intrinsically unstructured"1, and “intrinsically disordered”2. The failure to self-fold into specific 3-D ...
PDF Datastream - Brown Digital Repository
... RsaA- using Q-TOF or LTQ-Orbitrap……………………...............................................81 Figure 3.8: Functional classification of total 91 putative surface-exposed proteins identified using both Q-TOF and LTQ-Orbitrap method..................................................82 Figure 3.9: Distribu ...
... RsaA- using Q-TOF or LTQ-Orbitrap……………………...............................................81 Figure 3.8: Functional classification of total 91 putative surface-exposed proteins identified using both Q-TOF and LTQ-Orbitrap method..................................................82 Figure 3.9: Distribu ...
Recognition Specificity for the Bacterial Avirulence Protein AvrPto Is
... will be required to test this possibility because tobacco ...
... will be required to test this possibility because tobacco ...
Single-molecule super-resolution microscopy (STORM)
... myosin based intracellular transport, cell division, cell surface based movement and the ability of cells to adopt a variety of shapes. The actin cytoskeleton is made up of actin filaments decorated by a variety of actinbinding proteins. Actin-binding proteins play a fundamental role in the architec ...
... myosin based intracellular transport, cell division, cell surface based movement and the ability of cells to adopt a variety of shapes. The actin cytoskeleton is made up of actin filaments decorated by a variety of actinbinding proteins. Actin-binding proteins play a fundamental role in the architec ...
HIC-5 Is a Novel Repressor of Lymphoid Enhancer Factor/T
... Activation of Wnt/-catenin target genes is regulated by a heterodimer of -catenin and the high mobility group box transcription factors of the lymphoid enhancer factor (LEF)/T-cell factor (TCF) family. In vertebrates, four LEF/TCF family members have been identified. They all contain a conserved  ...
... Activation of Wnt/-catenin target genes is regulated by a heterodimer of -catenin and the high mobility group box transcription factors of the lymphoid enhancer factor (LEF)/T-cell factor (TCF) family. In vertebrates, four LEF/TCF family members have been identified. They all contain a conserved  ...
Non-coding RNAs - Biology Department | UNC Chapel Hill
... Figure 2 | Biogenesis of Sm-class small nuclear RNPs. The life-cycle of Sm-class small nuclear ribonucleoproteins (snRNPs) includes both nuclear and cytoplasmic phases. Following transcription by a specialized form of RNA polymerase II, pre-snRNA transcripts are exported to the cytoplasm, with a pot ...
... Figure 2 | Biogenesis of Sm-class small nuclear RNPs. The life-cycle of Sm-class small nuclear ribonucleoproteins (snRNPs) includes both nuclear and cytoplasmic phases. Following transcription by a specialized form of RNA polymerase II, pre-snRNA transcripts are exported to the cytoplasm, with a pot ...
evolution of protein function by domain swapping
... and Schirmer, 1979). The term subdomain usually refers to units smaller than a domain that can be described as having a particular function or structure (i.e., some rationale for being grouped together). What distinguishes the two from each other can often be confusing and the terms are frequently u ...
... and Schirmer, 1979). The term subdomain usually refers to units smaller than a domain that can be described as having a particular function or structure (i.e., some rationale for being grouped together). What distinguishes the two from each other can often be confusing and the terms are frequently u ...
New Insights Into HCV Replication: Potential Antiviral - IAS-USA
... have also received considerable attention. The NS4B protein may scaffold the RNA replication machinery and is sufficient to form the membranous web. It is the target of several compounds recently identified in novel biochemical assays. The NS5A protein is involved in both RNA replication and infecti ...
... have also received considerable attention. The NS4B protein may scaffold the RNA replication machinery and is sufficient to form the membranous web. It is the target of several compounds recently identified in novel biochemical assays. The NS5A protein is involved in both RNA replication and infecti ...
The evolution, function, structure, and expression
... are conserved. Comparisons of the sHSPs from diverse organisms reveal conserved structural features including an oligomeric form with a β-sandwich that forms a hollow ball. This conservation occurs despite significant divergence in primary sequences. It is well established that sHSPs are molecular c ...
... are conserved. Comparisons of the sHSPs from diverse organisms reveal conserved structural features including an oligomeric form with a β-sandwich that forms a hollow ball. This conservation occurs despite significant divergence in primary sequences. It is well established that sHSPs are molecular c ...
The Case Against a Darwinian Origin of Protein Folds
... other aspects of proteins will need to be examined before we can decide whether their size complicates Darwinian explanations of their origins. The simple relationship between gene sequences and protein sequences in bacteria allows protein sizes to be determined directly from genomic data. This, in ...
... other aspects of proteins will need to be examined before we can decide whether their size complicates Darwinian explanations of their origins. The simple relationship between gene sequences and protein sequences in bacteria allows protein sizes to be determined directly from genomic data. This, in ...
Crystal Structures of Two Viral IRES RNA Domains Bound to the
... normal growth under physiological conditions as well as responses to internal or external stresses. Viruses do not have their own translation apparatus and have to use the host’s ribosome to synthesize their viral proteins. During viral infections, host cells down-regulate capdependent initiation as ...
... normal growth under physiological conditions as well as responses to internal or external stresses. Viruses do not have their own translation apparatus and have to use the host’s ribosome to synthesize their viral proteins. During viral infections, host cells down-regulate capdependent initiation as ...
PDF
... organs when tadpoles and frogs of R. catesbeiana are compared (Manwell, 1966). Acid phosphatase, lipase, and N-benzoyl-arginine-naphthylamide cathepsin are distinct single electrophoretic zones which change only quantitatively during development and are not altered by partial decapitation, at least ...
... organs when tadpoles and frogs of R. catesbeiana are compared (Manwell, 1966). Acid phosphatase, lipase, and N-benzoyl-arginine-naphthylamide cathepsin are distinct single electrophoretic zones which change only quantitatively during development and are not altered by partial decapitation, at least ...
Protein Kinase A Regulatory Subunit Interacts with P
... contain eight conserved motifs solvated in the cytoplasm, where ATP binding and hydrolysis occur.11,12 The primary function of Na+ -K+-ATPase is to transport ions across the cell membrane. However, some mammalian Na+-K+-ATPase α-subunits contains multiple well-characterized protein-binding motifs, a ...
... contain eight conserved motifs solvated in the cytoplasm, where ATP binding and hydrolysis occur.11,12 The primary function of Na+ -K+-ATPase is to transport ions across the cell membrane. However, some mammalian Na+-K+-ATPase α-subunits contains multiple well-characterized protein-binding motifs, a ...
Metabolic and physiological interdependencies in the
... Figure 2 Density gradient enrichment of symbiont cells and host components followed by CARD-FISH. (a) Step-wise workflow of the density gradient enrichment method for physical separation of B. azoricus host and symbiont cells. Sampling for tissue-based metaproteomic analysis of whole gill and foot t ...
... Figure 2 Density gradient enrichment of symbiont cells and host components followed by CARD-FISH. (a) Step-wise workflow of the density gradient enrichment method for physical separation of B. azoricus host and symbiont cells. Sampling for tissue-based metaproteomic analysis of whole gill and foot t ...
Initiation of transcription by Pol II Separate basal and activated
... Activated transcription by Pol II enhancers are sequences 5’ to TATAA transcriptional activators bind them • have distinct DNA binding and activation domains • activation domain interacts with mediator • helps assemble initiation complex on TATAA ...
... Activated transcription by Pol II enhancers are sequences 5’ to TATAA transcriptional activators bind them • have distinct DNA binding and activation domains • activation domain interacts with mediator • helps assemble initiation complex on TATAA ...
Nucleoli: Composition, Function, and Dynamics
... organizer regions (NORs) of the chromosomes (Raska et al., 2006a, 2006b). The rDNA repeats are transcribed as a single precursor RNA, which is edited into the three rRNAs by excising leading, internal, and trailing transcribed spacer sequences. The rDNA repeats are separated by untranscribed interge ...
... organizer regions (NORs) of the chromosomes (Raska et al., 2006a, 2006b). The rDNA repeats are transcribed as a single precursor RNA, which is edited into the three rRNAs by excising leading, internal, and trailing transcribed spacer sequences. The rDNA repeats are separated by untranscribed interge ...
COMPUTATIONAL PERSPECTIVE IN THE STRUCTURAL STABILITY OF ‘ALLALPHA’ PROTEINS: THE NH...Π INTERACTIONS
... have carried out data base analysis to establish the stability of proteins. A folded protein is stabilized by a number of noncovalent interactions such as hydrophobic interactions, hydrogen bonds, salt bridges, and cation‐aromatic interactions 1,2. In addition, there has bee ...
... have carried out data base analysis to establish the stability of proteins. A folded protein is stabilized by a number of noncovalent interactions such as hydrophobic interactions, hydrogen bonds, salt bridges, and cation‐aromatic interactions 1,2. In addition, there has bee ...
The Role of the Plant Nucleolus in Pre-mRNA Processing
... nucleolus is also involved in some virus infections (both animal and plant), with particular viral proteins localising to the nucleolus (Hiscox 2002, 2007). Although the function of the nucleolar localisation of many of these proteins remains to be established, the nucleolar accumulation of the ORF3 ...
... nucleolus is also involved in some virus infections (both animal and plant), with particular viral proteins localising to the nucleolus (Hiscox 2002, 2007). Although the function of the nucleolar localisation of many of these proteins remains to be established, the nucleolar accumulation of the ORF3 ...
Protein Arginine Methylation in Candida albicans: Role
... Protein arginine methylation plays a key role in numerous eukaryotic processes, such as protein transport and signal transduction. In Candida albicans, two candidate protein arginine methyltransferases (PRMTs) have been identified from the genome sequencing project. Based on sequence comparison, C. ...
... Protein arginine methylation plays a key role in numerous eukaryotic processes, such as protein transport and signal transduction. In Candida albicans, two candidate protein arginine methyltransferases (PRMTs) have been identified from the genome sequencing project. Based on sequence comparison, C. ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.