Translation I
... anti-codon stem loop interacts with both the C-terminal domain and the -helical domain (pink). The tRNA makes no contact with the catalytic domain of the same subunit (cyan). (B) The unusual conformation of the anti-codon triplet in which Ade-36 is stacked on Gua-34, while Psu-35 bulges out. (C) Bas ...
... anti-codon stem loop interacts with both the C-terminal domain and the -helical domain (pink). The tRNA makes no contact with the catalytic domain of the same subunit (cyan). (B) The unusual conformation of the anti-codon triplet in which Ade-36 is stacked on Gua-34, while Psu-35 bulges out. (C) Bas ...
Q. No. 1. How can RNA be distinguished from DNA?
... Ans. RNA and DNA are both nucleic acids, but differ in three main ways. First, unlike DNA which is generally double-stranded, RNA is a single-stranded molecule in many of its biological roles and has a much shorter chain of nucleotides. Second, while DNA contains deoxyribose, RNA contains ribose (th ...
... Ans. RNA and DNA are both nucleic acids, but differ in three main ways. First, unlike DNA which is generally double-stranded, RNA is a single-stranded molecule in many of its biological roles and has a much shorter chain of nucleotides. Second, while DNA contains deoxyribose, RNA contains ribose (th ...
Protein engineering: navigating between chance and reason
... space. Whereas cell-free systems can be used to churn through greater numbers of proteins (including proteins toxic to yeast and bacteria), cell-based systems offer a ready means of production, particularly for large, complex proteins. Researchers including George Georgiou at the University of Texas ...
... space. Whereas cell-free systems can be used to churn through greater numbers of proteins (including proteins toxic to yeast and bacteria), cell-based systems offer a ready means of production, particularly for large, complex proteins. Researchers including George Georgiou at the University of Texas ...
Carbon Compounds 2-3 Foldable Instructions
... & lipids. It turns red in the presence of fats & lipids. Benedict’s solution is an indicator solution for simple sugars. It changes from blue to yellow, orange or red. Iodine solution is an indicator solution for complex sugars. It changes from brown to blue ...
... & lipids. It turns red in the presence of fats & lipids. Benedict’s solution is an indicator solution for simple sugars. It changes from blue to yellow, orange or red. Iodine solution is an indicator solution for complex sugars. It changes from brown to blue ...
Transcription Translation
... 5’cap Helps with export from nucleus Protection from degradation of enzymes Attachment to ribosomes ...
... 5’cap Helps with export from nucleus Protection from degradation of enzymes Attachment to ribosomes ...
Lecture exam 1A
... E. None of the above is correct 3. Which of the following statements, if any, is FALSE? If none of A-D are false select E. A. Protein folding often occurs spontaneously B. Chaperones are specific proteins involved in protein folding C. The final tertiary structure of a protein is dictated by its ami ...
... E. None of the above is correct 3. Which of the following statements, if any, is FALSE? If none of A-D are false select E. A. Protein folding often occurs spontaneously B. Chaperones are specific proteins involved in protein folding C. The final tertiary structure of a protein is dictated by its ami ...
7.5 Proteins - HS Biology IB
... determined by H bonds (within chain); contributes to the strength of fibrous proteins; tertiary structure refers to overall 3-D shape; conformation can determine function; tertiary structure determined by R-group interactions / ionic interactions / hydrophobic interactions / disulfide bridges / H-bo ...
... determined by H bonds (within chain); contributes to the strength of fibrous proteins; tertiary structure refers to overall 3-D shape; conformation can determine function; tertiary structure determined by R-group interactions / ionic interactions / hydrophobic interactions / disulfide bridges / H-bo ...
Week 16
... Nutlin-MDM2 binding activates P53 • Nutlins are cis-imidazoline analogs that can inhibit the interaction between MDM2 and tumor suppressor P53, a tumor suppressor gene • It stabilizes P53, which induces senescence (growthinhibition) in cancer cells. • Nutlin family of small molecules are widely ...
... Nutlin-MDM2 binding activates P53 • Nutlins are cis-imidazoline analogs that can inhibit the interaction between MDM2 and tumor suppressor P53, a tumor suppressor gene • It stabilizes P53, which induces senescence (growthinhibition) in cancer cells. • Nutlin family of small molecules are widely ...
Proteins in nutrition
... about 1,2 – 1,5 g / kg / day Pregnancy and breastfeeding: about 1,5 g / kg / day Sportsmen in training: max. 1,3 – 1,5 g / kg / day (higher amount do not use for proteosynthesis, but only as energy source) Excessive intake: The human body is able to use for proteosynthesis only limited amount of ami ...
... about 1,2 – 1,5 g / kg / day Pregnancy and breastfeeding: about 1,5 g / kg / day Sportsmen in training: max. 1,3 – 1,5 g / kg / day (higher amount do not use for proteosynthesis, but only as energy source) Excessive intake: The human body is able to use for proteosynthesis only limited amount of ami ...
Cell signaling
... •Protein kinases are enzymes that add a phosphate group from ATP onto a substrate protein; this reaction is called phosphorylation •Phosphorylation frequently serves to activate the substrate of the kinase, but can also target the substrate for degradation •Kinases are often themselves activated by ...
... •Protein kinases are enzymes that add a phosphate group from ATP onto a substrate protein; this reaction is called phosphorylation •Phosphorylation frequently serves to activate the substrate of the kinase, but can also target the substrate for degradation •Kinases are often themselves activated by ...
Introduction to Proteomics
... must not be dialysed. (halobacteria lysate) – Some proteins will gel if desalted. (Bovine vitreous proteins) Solution for above: low voltage (100V) for 5 hours before IEF running. (A. Gorg, 1995) ...
... must not be dialysed. (halobacteria lysate) – Some proteins will gel if desalted. (Bovine vitreous proteins) Solution for above: low voltage (100V) for 5 hours before IEF running. (A. Gorg, 1995) ...
Neuregulin-1 Alleviated Doxorubicin-Induced Down
... doxorubicin. These effects of NRG1 depended on the erbB2 receptor, as well as the PI3K, Akt and mTOR pathways, but not by the erbB4 receptor, PKC or p38. These results demonstrated that NRG1 restored the levels of cTnI and cTnT by increasing the transcription and translation, as well as by decreasin ...
... doxorubicin. These effects of NRG1 depended on the erbB2 receptor, as well as the PI3K, Akt and mTOR pathways, but not by the erbB4 receptor, PKC or p38. These results demonstrated that NRG1 restored the levels of cTnI and cTnT by increasing the transcription and translation, as well as by decreasin ...
Evolution connection: proteins, carbohydrates, and nucleic acids
... translation of nucleic acids into proteins could be incorporated into the last slide of this series, to the degree that you cover this material in your discussion of the structure of nucleic acids. You may also wish to return to this story as an example when you cover Mendelian genetics. The Europea ...
... translation of nucleic acids into proteins could be incorporated into the last slide of this series, to the degree that you cover this material in your discussion of the structure of nucleic acids. You may also wish to return to this story as an example when you cover Mendelian genetics. The Europea ...
Peptide Sequencing by Mass Spectrometry
... Describe the steps of a typical peptide analysis by MS (proteomic experiment) Explain peptide ionization, fragmentation, identification ...
... Describe the steps of a typical peptide analysis by MS (proteomic experiment) Explain peptide ionization, fragmentation, identification ...
Chapter Five
... Protein may be sacrificed to provide energy if insufficient carbohydrate and fat are ...
... Protein may be sacrificed to provide energy if insufficient carbohydrate and fat are ...
The use of isotope-coded affinity tags (ICAT)
... To date, no comprehensive study of any component of the plant endomembrane system has been reported, reflecting the difficulty in purifying the component organelles which have similar sizes and densities. However, proteomic studies of a component of the animal endomembrane system, the Golgi apparatu ...
... To date, no comprehensive study of any component of the plant endomembrane system has been reported, reflecting the difficulty in purifying the component organelles which have similar sizes and densities. However, proteomic studies of a component of the animal endomembrane system, the Golgi apparatu ...
Eight bacterial proteins, including UDP-N
... Fig. 2. The imperfect tandem hexapeptide repeat structure of eight bacterial proteins. The hexapeptide arrangement was revealed by locating those IG, LG, and VG dipeptide residues which are followed or preceded by lie, Leu, or Val at an [I,V,LHI,V,L] distance of six residues. Such dipeptides as well ...
... Fig. 2. The imperfect tandem hexapeptide repeat structure of eight bacterial proteins. The hexapeptide arrangement was revealed by locating those IG, LG, and VG dipeptide residues which are followed or preceded by lie, Leu, or Val at an [I,V,LHI,V,L] distance of six residues. Such dipeptides as well ...
Gene expression flash cards
... The view that nucleic acids / DNA determines protein structure is known as The Central Dogma ...
... The view that nucleic acids / DNA determines protein structure is known as The Central Dogma ...
Protein degradation and regulation
... targeting for degradation cellular substrates which may interfere with propagation of the virus. In some instances, the viral protein functions as a bridging‘ element between the E3 and the substrate, thus conferring recognition in trans. The prototype of such a protein is the high risk HPV oncoprot ...
... targeting for degradation cellular substrates which may interfere with propagation of the virus. In some instances, the viral protein functions as a bridging‘ element between the E3 and the substrate, thus conferring recognition in trans. The prototype of such a protein is the high risk HPV oncoprot ...
In silico Study of Target Proteins for Mycobacterium
... The completion of the genome of pathogens and the human has provided data that can be utilized to design vaccines and drug targets. One of the recently adopted strategies for drug designing is based on comparative genomics approach, it gives a set of genes that are likely to be essential to the path ...
... The completion of the genome of pathogens and the human has provided data that can be utilized to design vaccines and drug targets. One of the recently adopted strategies for drug designing is based on comparative genomics approach, it gives a set of genes that are likely to be essential to the path ...
Prestained Protein Molecular Weight Marker
... Load Volume 0.75mm – thick mini gels – 5µl 1.5mm – thick mini gels – 10µl 0.75mm – thick standard gels – 10µl 1.5mm – thick standard gels – 20µl Instruction for Use 1. Thaw the Marker at room temperature or heat at 37- 40°C for a few minutes to dissolve precipitated solids. Do not boil! 2. Vortex ge ...
... Load Volume 0.75mm – thick mini gels – 5µl 1.5mm – thick mini gels – 10µl 0.75mm – thick standard gels – 10µl 1.5mm – thick standard gels – 20µl Instruction for Use 1. Thaw the Marker at room temperature or heat at 37- 40°C for a few minutes to dissolve precipitated solids. Do not boil! 2. Vortex ge ...
Chemical Approaches for Quantitative and Functional Proteomics
... Activity Based Protein Profiling • Abundance methods are indirect estimates of protein function. • ABP Probes report on the functional state of enzymes in ...
... Activity Based Protein Profiling • Abundance methods are indirect estimates of protein function. • ABP Probes report on the functional state of enzymes in ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.