Towards evolving a better repressor
... (2001) created an ‘optimal inducible system’ where lac provided tight, inducible and reversible regulation of the tyrosinase gene involved in mouse pigmentation. Two lines of transgenic mice were created: one cell line expressed the lac repressor and the other expressed the tyrosinase gene under the ...
... (2001) created an ‘optimal inducible system’ where lac provided tight, inducible and reversible regulation of the tyrosinase gene involved in mouse pigmentation. Two lines of transgenic mice were created: one cell line expressed the lac repressor and the other expressed the tyrosinase gene under the ...
DNA Extraction from Extremophiles - Center for Ribosomal Origins
... genetic information that underlies heredity is both encoded in genes (as a string of molecular “letters”) and replicated (by a templating mechanism). Each DNA molecule in a cell forms a single chromosome. Changes in DNA (mutations) occur spontaneously at low rates. Some of these changes make no diff ...
... genetic information that underlies heredity is both encoded in genes (as a string of molecular “letters”) and replicated (by a templating mechanism). Each DNA molecule in a cell forms a single chromosome. Changes in DNA (mutations) occur spontaneously at low rates. Some of these changes make no diff ...
Cloning, Characterization, and Chromosomal Mapping of Human
... We recently cloned a cDNA of the collecting duct apical membrane water channel of rat kidney, which is important for the formation of concentrated urine (Fushima, K., S. Uchida, Y. Hara, Y. Hirata, F. Marumo, and S. Sasaki. 1993. Nature I Lond.I. 361:549-552). Since urine concentrating ability varie ...
... We recently cloned a cDNA of the collecting duct apical membrane water channel of rat kidney, which is important for the formation of concentrated urine (Fushima, K., S. Uchida, Y. Hara, Y. Hirata, F. Marumo, and S. Sasaki. 1993. Nature I Lond.I. 361:549-552). Since urine concentrating ability varie ...
Synthetic Plant Promoters Containing Defined Regulatory Elements
... there were major differences in the inducibilities of the various promoters with the pathogens tested as well as differences in the speed of induction and in the basal expression levels. We also show that defense signaling is largely conserved across species boundaries at the cis-acting element leve ...
... there were major differences in the inducibilities of the various promoters with the pathogens tested as well as differences in the speed of induction and in the basal expression levels. We also show that defense signaling is largely conserved across species boundaries at the cis-acting element leve ...
Cloning, Functional Characterization and Site
... of core structure of coumarin in P. praeruptorum have not been identified yet. 4Coumarate: CoA ligase (4CL) catalyzes the formation of hydroxycinnamates CoA esters, and plays an essential role at the divergence point from general phenylpropanoid metabolism to major branch pathway of coumarin. Here, ...
... of core structure of coumarin in P. praeruptorum have not been identified yet. 4Coumarate: CoA ligase (4CL) catalyzes the formation of hydroxycinnamates CoA esters, and plays an essential role at the divergence point from general phenylpropanoid metabolism to major branch pathway of coumarin. Here, ...
The home stretch, a first analysis of the nearly completed genome of
... Figure 1. (a, b) These maps represent our current working model of the order of the DOE contigs around the two chromosomes. The black horizontal lines represent, to scale, the size of the contigs. Above each black line is the contig number. Small contigs have their numbers written vertically, e.g. t ...
... Figure 1. (a, b) These maps represent our current working model of the order of the DOE contigs around the two chromosomes. The black horizontal lines represent, to scale, the size of the contigs. Above each black line is the contig number. Small contigs have their numbers written vertically, e.g. t ...
Proteins As Drugs - The Major Products of the Biotechnology Industry
... • TNFα is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis (RA), polyarticular-course juvenile rheumatoid arthritis (JRA), and ankylosing spondylitis and the resulting joint p ...
... • TNFα is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis (RA), polyarticular-course juvenile rheumatoid arthritis (JRA), and ankylosing spondylitis and the resulting joint p ...
Molecular and Immunological Methods
... target amplicon. The simplest approach relies upon determining the melting temperature of the amplicon using a melting curve. The real time PCR is performed as normal, incorporating a non-hydrolysed probe or dye – typically performed with SYBR Green or a saturation dye such as SYTO 9 or LC Green 1. ...
... target amplicon. The simplest approach relies upon determining the melting temperature of the amplicon using a melting curve. The real time PCR is performed as normal, incorporating a non-hydrolysed probe or dye – typically performed with SYBR Green or a saturation dye such as SYTO 9 or LC Green 1. ...
Pol Is a Candidate for the Mouse Pulmonary
... United States (1). Although cigarette smoke is implicated in ⬃90% of male and 75– 80% of female lung cancer deaths, only ⬃15% of heavy smokers will ultimately develop lung cancer, which suggests that there is variation in individual susceptibility to lung cancer (2, 3). Inbred strains of mice vary m ...
... United States (1). Although cigarette smoke is implicated in ⬃90% of male and 75– 80% of female lung cancer deaths, only ⬃15% of heavy smokers will ultimately develop lung cancer, which suggests that there is variation in individual susceptibility to lung cancer (2, 3). Inbred strains of mice vary m ...
Synthetic Plant Promoters Containing Defined
... there were major differences in the inducibilities of the various promoters with the pathogens tested as well as differences in the speed of induction and in the basal expression levels. We also show that defense signaling is largely conserved across species boundaries at the cis-acting element leve ...
... there were major differences in the inducibilities of the various promoters with the pathogens tested as well as differences in the speed of induction and in the basal expression levels. We also show that defense signaling is largely conserved across species boundaries at the cis-acting element leve ...
- Horizon Discovery
... We have shown the increase in gene editing efficiency of rAAV donors when incorporating shRNA negative selection and supressing MSH2 gene expression during HR. When these improvements are used in combination, the boost in targeting frequency has improved the success of generating isogenic cell lines ...
... We have shown the increase in gene editing efficiency of rAAV donors when incorporating shRNA negative selection and supressing MSH2 gene expression during HR. When these improvements are used in combination, the boost in targeting frequency has improved the success of generating isogenic cell lines ...
Ex vivo analysis of splicing assays
... how to improve our understanding of new disease-causing mutations, that do not affect the coding potential of a gene, but that affect pre-mRNA splicing. In order to analyse precise mechanism of alternative splicing most laboratories use socalled mini-gene reporters. These reporters can easily be tra ...
... how to improve our understanding of new disease-causing mutations, that do not affect the coding potential of a gene, but that affect pre-mRNA splicing. In order to analyse precise mechanism of alternative splicing most laboratories use socalled mini-gene reporters. These reporters can easily be tra ...
Lecture 13 Lytic vs. Lysogenic cycles:
... gene produces a repressor protein that binds to its transcription factor and blocks the lytic pathway. Then the int gene produces integrase which allows recombination with the host DNA and eventually integration into it. The attachment points are called attP ...
... gene produces a repressor protein that binds to its transcription factor and blocks the lytic pathway. Then the int gene produces integrase which allows recombination with the host DNA and eventually integration into it. The attachment points are called attP ...
Molecular genetics of the carotenoid biosynthesis pathway in plants
... resistance trait in a wild-type strain of cyanobacteria with a genomic library from the mutant (ref. 7). The cyanobacterial genes were successfully used as molecular probes to clone the homologous genes from algae and higher plants (ref. 10-12). The cyanobacterial gene for phytoene synthase was disc ...
... resistance trait in a wild-type strain of cyanobacteria with a genomic library from the mutant (ref. 7). The cyanobacterial genes were successfully used as molecular probes to clone the homologous genes from algae and higher plants (ref. 10-12). The cyanobacterial gene for phytoene synthase was disc ...
Cloning and characterization of a phosphopantetheinyl transferase
... attachment of the P-pant group to a conserved serine residue of the carrier proteins. This posttranslational modi¢cation is catalyzed by a superfamily of enzymes known as phosphopantetheinyl transferases (PPTases) that derive the P-pant group from coenzyme A (CoA) [9,10]. In organisms containing mul ...
... attachment of the P-pant group to a conserved serine residue of the carrier proteins. This posttranslational modi¢cation is catalyzed by a superfamily of enzymes known as phosphopantetheinyl transferases (PPTases) that derive the P-pant group from coenzyme A (CoA) [9,10]. In organisms containing mul ...
Chapter 1
... gene as a very interesting tool to understand the complex network of gene regulation in guard cells. On the other hand, γVPE codes for a vacuolar processing enzyme with a caspase-1 like activity and substrate specificity towards asparagine residues. It belongs to the VPEs family, found in various eu ...
... gene as a very interesting tool to understand the complex network of gene regulation in guard cells. On the other hand, γVPE codes for a vacuolar processing enzyme with a caspase-1 like activity and substrate specificity towards asparagine residues. It belongs to the VPEs family, found in various eu ...
video slide
... – This cDNA represents only part of the genome – This is advantageous for; • Studying the genes responsible for specialized functions of a particular type of cells such as brain or liver cells. • In addition by making cDNA library from cells of same type at different stages of life of an organism, r ...
... – This cDNA represents only part of the genome – This is advantageous for; • Studying the genes responsible for specialized functions of a particular type of cells such as brain or liver cells. • In addition by making cDNA library from cells of same type at different stages of life of an organism, r ...
Site-directed Mutagenesis of Arginine
... complex and the Escherichia coli TS' dUMp· lO-propaa series of 11 site-directed mutations at Arg-178 in rgyl-S,8-dideazafolate ternary complex have provided a single experiment The use of synthetic gene is furinsight about the possible roles of specific residues ther enhanced by its high level ·of e ...
... complex and the Escherichia coli TS' dUMp· lO-propaa series of 11 site-directed mutations at Arg-178 in rgyl-S,8-dideazafolate ternary complex have provided a single experiment The use of synthetic gene is furinsight about the possible roles of specific residues ther enhanced by its high level ·of e ...
Receptors
... Both the A/B domains and E/F domains have transcriptional activity Different steroid receptors are continually being discovered ...
... Both the A/B domains and E/F domains have transcriptional activity Different steroid receptors are continually being discovered ...
FTv6_6_changes
... between the indicated points). From October 2006 the usage of this descriptor is restricted: it is illegal to use "a single base from a range" (c) either on its own or in combination with the "sequence span" (d) descriptor for newly created entries. The existing entries where such combinations exist ...
... between the indicated points). From October 2006 the usage of this descriptor is restricted: it is illegal to use "a single base from a range" (c) either on its own or in combination with the "sequence span" (d) descriptor for newly created entries. The existing entries where such combinations exist ...
PDF handout
... • The geometric orientations of the catalytic residues are similar between families, despite different protein folds. • The linear arrangements of the catalytic residues reflect different family relationships. For example the catalytic triad in the chymotrypsin clan is ordered HDS, but is ordered DH ...
... • The geometric orientations of the catalytic residues are similar between families, despite different protein folds. • The linear arrangements of the catalytic residues reflect different family relationships. For example the catalytic triad in the chymotrypsin clan is ordered HDS, but is ordered DH ...
A prophage-encoded actin-like protein required for efficient viral
... The relative amount of circular phage DNA was determined via quantitative PCR (qPCR). Therefore, C. glutamicum wild type, the alpC deletion strain, and the alpA deletion strain were grown in 5 ml BHI (Brain Heart Infusion, Difco) for about 6 h at 30◦ C. A second precultivation was performed in CGXII ...
... The relative amount of circular phage DNA was determined via quantitative PCR (qPCR). Therefore, C. glutamicum wild type, the alpC deletion strain, and the alpA deletion strain were grown in 5 ml BHI (Brain Heart Infusion, Difco) for about 6 h at 30◦ C. A second precultivation was performed in CGXII ...