Chapter 16
... • Provides a “hook” upon which DNA polymerase can “hang” the nucleotides needed to elongate the new DNA strand • RNA “hook” called the “Primer” ...
... • Provides a “hook” upon which DNA polymerase can “hang” the nucleotides needed to elongate the new DNA strand • RNA “hook” called the “Primer” ...
Technical White Paper SOMAmer® Reagent Specificity
... SOMAmer reagent specificity in the SOMAscan assay The highly multiplexed SOMAscan assay takes advantage of both of the specificity attributes of SOMAmer reagents described above (high affinity and slow dissociation rates for cognate proteins), and includes assay steps to maximize that specificity. F ...
... SOMAmer reagent specificity in the SOMAscan assay The highly multiplexed SOMAscan assay takes advantage of both of the specificity attributes of SOMAmer reagents described above (high affinity and slow dissociation rates for cognate proteins), and includes assay steps to maximize that specificity. F ...
Introduction to molecular and cell biology
... • RNAz calculates a normalized measure of thermodynamic stability by – compares the MFE m of a given (native) sequence – with the MFEs of a large number of random sequences with similar length and base composition. ...
... • RNAz calculates a normalized measure of thermodynamic stability by – compares the MFE m of a given (native) sequence – with the MFEs of a large number of random sequences with similar length and base composition. ...
Structure-Function Analysis of the UDP-N-acetyl-D
... Recently, several groups have identified an aspartate-any residue-aspartate (DXD) (or aspartate-any residue-histidine (DXH)) sequence motif that is common to many glycosyltransferases (6 –9). All known ppGaNTases contain such a DXH or DXD-like motif. Based on fold recognition, the DXD motif of the a ...
... Recently, several groups have identified an aspartate-any residue-aspartate (DXD) (or aspartate-any residue-histidine (DXH)) sequence motif that is common to many glycosyltransferases (6 –9). All known ppGaNTases contain such a DXH or DXD-like motif. Based on fold recognition, the DXD motif of the a ...
invisible forms
... Nuclear MagneNc Resonance is a type of spectrometry in which proteins are hit with radio waves while they're in a strong magne3c field. Since atomic nuclei selec3vely absorb electromagne3c radia3ons, ...
... Nuclear MagneNc Resonance is a type of spectrometry in which proteins are hit with radio waves while they're in a strong magne3c field. Since atomic nuclei selec3vely absorb electromagne3c radia3ons, ...
ppt - Chair of Computational Biology
... Can disease-associated alleles be predicted from sequence? Main feature that distinguishes a map-based approach from a genome-based approach to genome-wide association studies is: degree to which functional variants can be predicted on the basis of sequence in, for example, coding and/or conserved ...
... Can disease-associated alleles be predicted from sequence? Main feature that distinguishes a map-based approach from a genome-based approach to genome-wide association studies is: degree to which functional variants can be predicted on the basis of sequence in, for example, coding and/or conserved ...
Small G-protein
... Small GTP-binding proteins include (roles indicated): Compared to the subunit of heterotrimeric G-proteins, GTPase activity of the monomeric G-proteins is very low in the absence of interference. However, association with a protein of the GAP (GTPase-activating protein) type results in very rapid ...
... Small GTP-binding proteins include (roles indicated): Compared to the subunit of heterotrimeric G-proteins, GTPase activity of the monomeric G-proteins is very low in the absence of interference. However, association with a protein of the GAP (GTPase-activating protein) type results in very rapid ...
Addition of the keto functional group to the genetic
... Asp-158, Ile-159, and Leu-162) in the active site of M. jannaschii TyrRS were randomly mutated by PCR to generate a library 1.6 ⫻ 109 in size (4). This TyrRS mutant library was first passed through a positive selection in the presence of 1 mM p-acetylL-phenylalanine, which is based on the suppressio ...
... Asp-158, Ile-159, and Leu-162) in the active site of M. jannaschii TyrRS were randomly mutated by PCR to generate a library 1.6 ⫻ 109 in size (4). This TyrRS mutant library was first passed through a positive selection in the presence of 1 mM p-acetylL-phenylalanine, which is based on the suppressio ...
Lecture 12 Proteins Classification of protein Proteins are classified
... These are formed by the action of acids and alkalies upon protein. They are insoluble in neutral solvents. Coagulated proteins Coagulated proteins are insoluble products formed by the action of heat or alcohol on natural proteins e.g., cooked meat and cooked albumin. Secondary-derived protei ...
... These are formed by the action of acids and alkalies upon protein. They are insoluble in neutral solvents. Coagulated proteins Coagulated proteins are insoluble products formed by the action of heat or alcohol on natural proteins e.g., cooked meat and cooked albumin. Secondary-derived protei ...
Lachiewicz, AM, Spiridigliozzi, GA, McConkie-Rosell, A, Burgess, D, Feng, Y, Warren, ST and Tarleton, J: A fragile X male with a broad smear on Southern blot analysis representing 100 to 500 CGG repeats and no methylation of the Eag I site of the FMR1 gene. American Journal of Medical Genetics 64:278-282 (1996).
... higher le~els than carriers of the full mutation [Loesch et al., 1993; McConkie-Rosell et al., 1993; Staley et al., 1993; Rousseau et al., 1994b]. Hagerman et al. [1994] reviewed DNA findings on 29 males with fragile X syndrome who had IQs greater than 70. These males represented almost 12% (29/250) ...
... higher le~els than carriers of the full mutation [Loesch et al., 1993; McConkie-Rosell et al., 1993; Staley et al., 1993; Rousseau et al., 1994b]. Hagerman et al. [1994] reviewed DNA findings on 29 males with fragile X syndrome who had IQs greater than 70. These males represented almost 12% (29/250) ...
Polymerase Chain Reaction (PCR)
... material used. 3- Extraction mean breaking down the cell wall and membranes to allow access to nuclear material, with out its degradation. ...
... material used. 3- Extraction mean breaking down the cell wall and membranes to allow access to nuclear material, with out its degradation. ...
Salman Razzaque - The Gene Patent Problem: Recommendations for Congress
... Such a precedent will not cause companies to sue academic institutions, but may increase demands from patent holders for license fees, which will only burden researchers. Another problem of patents in academic research is that it hinders the basic characteristics of research. Namely, scientific obj ...
... Such a precedent will not cause companies to sue academic institutions, but may increase demands from patent holders for license fees, which will only burden researchers. Another problem of patents in academic research is that it hinders the basic characteristics of research. Namely, scientific obj ...
Protein-RNA interactions: Structural analysis and functional classes
... that bind different functional classes of RNA molecules, which is the focus of the current study. Many studies have characterized protein–DNA interactions,e.g.3–5 which have led to new methods for the prediction of DNA binding sites on protein structures.e.g.6–8 As more structural data has become av ...
... that bind different functional classes of RNA molecules, which is the focus of the current study. Many studies have characterized protein–DNA interactions,e.g.3–5 which have led to new methods for the prediction of DNA binding sites on protein structures.e.g.6–8 As more structural data has become av ...
Tissue-specific expression of tomato Ribonuclease LX during
... demonstrates the tight regulation of RNaseLX and RNaseLE by phosphate availability (Fig. 1B). Isolation and structural analysis of the RNaseLX gene For functional analysis of the gene, RNaseLX promoter sequences were cloned using a PCR assisted approach adopted from the 59-RACE technique as describe ...
... demonstrates the tight regulation of RNaseLX and RNaseLE by phosphate availability (Fig. 1B). Isolation and structural analysis of the RNaseLX gene For functional analysis of the gene, RNaseLX promoter sequences were cloned using a PCR assisted approach adopted from the 59-RACE technique as describe ...
Lipids 44:
... ARA constitutes about 60% of total fatty acids (TFA) and over 95% of cellular ARA is deposited in TAG in cytoplasmic lipid bodies [12]. The biosynthesis of VLC-PUFAs in algae follows various pathways, initiating from oleic acid exported from the chloroplast and employing polar extraplastidial lipids ...
... ARA constitutes about 60% of total fatty acids (TFA) and over 95% of cellular ARA is deposited in TAG in cytoplasmic lipid bodies [12]. The biosynthesis of VLC-PUFAs in algae follows various pathways, initiating from oleic acid exported from the chloroplast and employing polar extraplastidial lipids ...
Restriction enzyme
... consist of a related pair of enzymes – Endonuclease – cuts foreign DNA – Methylase – protects host DNA ...
... consist of a related pair of enzymes – Endonuclease – cuts foreign DNA – Methylase – protects host DNA ...
Expression and V (D) J recombination activity of mutated RAG
... immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination fun ...
... immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination fun ...
Hereditary Myopathy with Lactic Acidosis
... coded for the Fe-S cluster assembly protein ISCU and an intronic base pair substitution (g.7044G>C) was identified in the last intron of this gene. The mutation gave rise to the insertion of intron sequence into the mRNA, leading to a protein containing 15 abberant amino acids and a premature stop. ...
... coded for the Fe-S cluster assembly protein ISCU and an intronic base pair substitution (g.7044G>C) was identified in the last intron of this gene. The mutation gave rise to the insertion of intron sequence into the mRNA, leading to a protein containing 15 abberant amino acids and a premature stop. ...
Lynx - SAT 2012
... SAT Solvers “are a black-box” Problem Users want more Control • The story so far … • SAT solvers have been amazingly successful in many fields • AI, formal methods, testing, program analysis,… ...
... SAT Solvers “are a black-box” Problem Users want more Control • The story so far … • SAT solvers have been amazingly successful in many fields • AI, formal methods, testing, program analysis,… ...
Serological and molecular techniques to detect and identify plant
... produces an epitope-specific antibody. The IgG produced by the one cell line will be uniform and monospecific. Monoclonal antibodies have the advantage of being highly specific and can detect variation within serogroups constructed through the use of polyclonal antibodies. However, their production ...
... produces an epitope-specific antibody. The IgG produced by the one cell line will be uniform and monospecific. Monoclonal antibodies have the advantage of being highly specific and can detect variation within serogroups constructed through the use of polyclonal antibodies. However, their production ...
What is function
... Gene Ontology - flexibility • Imagine • protein 1 phosphorylates protein 2 • protein 2 binds to protein 3 (which then binds to DNA) • proteins 1, 2, or 3 may be coded on nearby genes • makes sense in terms of regulation / protein production • different metabolic functions • part of same "cellular p ...
... Gene Ontology - flexibility • Imagine • protein 1 phosphorylates protein 2 • protein 2 binds to protein 3 (which then binds to DNA) • proteins 1, 2, or 3 may be coded on nearby genes • makes sense in terms of regulation / protein production • different metabolic functions • part of same "cellular p ...
The Escherichia coli SlyD Is a Metal Ion-regulated Peptidyl
... Purification of SlyD, the N-terminal Fragment of SlyD (Amino Acids 1–146) and SlpA—SlyD was purified from overexpression cultures with a yield of 54 mg of homogenous protein/liter of culture. By silver staining, only a single band could be detected in SDS-PAGE after the last purification step. Amino ...
... Purification of SlyD, the N-terminal Fragment of SlyD (Amino Acids 1–146) and SlpA—SlyD was purified from overexpression cultures with a yield of 54 mg of homogenous protein/liter of culture. By silver staining, only a single band could be detected in SDS-PAGE after the last purification step. Amino ...
The O 2
... • Proteins need to have two or more polypeptide chains to function properly. • Each individual peptide is called subunit. ...
... • Proteins need to have two or more polypeptide chains to function properly. • Each individual peptide is called subunit. ...
DNA
... the B form. It has 10 base pairs per turn and major and minor grooves. When there is less water available and binds to some proteins, DNA is often in the A form. it has 11 base pairs per turn, so it is more compact. Z DNA forms when the DNA sequence has alternating purines and pyrimidines and ther ...
... the B form. It has 10 base pairs per turn and major and minor grooves. When there is less water available and binds to some proteins, DNA is often in the A form. it has 11 base pairs per turn, so it is more compact. Z DNA forms when the DNA sequence has alternating purines and pyrimidines and ther ...