evolution of protein function by domain swapping
... and Schirmer, 1979). The term subdomain usually refers to units smaller than a domain that can be described as having a particular function or structure (i.e., some rationale for being grouped together). What distinguishes the two from each other can often be confusing and the terms are frequently u ...
... and Schirmer, 1979). The term subdomain usually refers to units smaller than a domain that can be described as having a particular function or structure (i.e., some rationale for being grouped together). What distinguishes the two from each other can often be confusing and the terms are frequently u ...
Molecular Beacon Product Sheet
... prepare chimeric 2‟-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhanced binding to the RNA target, are substrates for RNase H, and are highly nuclease resistant (1). Fluorine has an interesting combination of properties, combining electronegativity similar to a hydroxyl group with siz ...
... prepare chimeric 2‟-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhanced binding to the RNA target, are substrates for RNase H, and are highly nuclease resistant (1). Fluorine has an interesting combination of properties, combining electronegativity similar to a hydroxyl group with siz ...
Testis-specific TAF homologs collaborate to control a
... proteins that might collaborate with the dTAF5 homolog can to regulate expression of specific target genes in Drosophila spermatocytes, we investigated the expression and function of other TAFII homologs in the Drosophila genome. We show that Drosophila primary spermatocytes express several addition ...
... proteins that might collaborate with the dTAF5 homolog can to regulate expression of specific target genes in Drosophila spermatocytes, we investigated the expression and function of other TAFII homologs in the Drosophila genome. We show that Drosophila primary spermatocytes express several addition ...
Predicted function of the vaccinia virus G5R protein
... each protein associated with the intracellular mature virion using mass spectrometry were unable to detect G5R within the intracellular mature virion (Yoder et al., 2006; Chung et al., 2006). When we employed a hidden Markov model (HMM) comparison search tool (HHsearch) to screen for the functions o ...
... each protein associated with the intracellular mature virion using mass spectrometry were unable to detect G5R within the intracellular mature virion (Yoder et al., 2006; Chung et al., 2006). When we employed a hidden Markov model (HMM) comparison search tool (HHsearch) to screen for the functions o ...
TriFecta Dicer-Substrate RNAi Manual
... take advantage of the link between Dicer and RISC loading that occurs when RNAs are processed by Dicer. Traditional 21-mer siRNAs are chemically synthesized RNA duplexes that mimic Dicer products and bypass the need for Dicer processing. Dicer-Substrate RNAs are chemically synthesized 27-mer RNA dup ...
... take advantage of the link between Dicer and RISC loading that occurs when RNAs are processed by Dicer. Traditional 21-mer siRNAs are chemically synthesized RNA duplexes that mimic Dicer products and bypass the need for Dicer processing. Dicer-Substrate RNAs are chemically synthesized 27-mer RNA dup ...
DNA polymerase active site is highly mutable
... MgCl2, 0.4 mg of activated calf thymus DNA, 10 M each dNTP, 0.25 mCi of [␣-32P]dATP, and 1 l of WT or mutant Taq pol. Incubations were at 72°C for 5 min, and reactions were stopped with the addition of 100 l of 0.1 M sodium pyrophosphate, followed by 0.5 ml of 10% trichloroacetic acid (TCA). Poly ...
... MgCl2, 0.4 mg of activated calf thymus DNA, 10 M each dNTP, 0.25 mCi of [␣-32P]dATP, and 1 l of WT or mutant Taq pol. Incubations were at 72°C for 5 min, and reactions were stopped with the addition of 100 l of 0.1 M sodium pyrophosphate, followed by 0.5 ml of 10% trichloroacetic acid (TCA). Poly ...
University of Groningen Structure and mechanism of the ECF
... the BioM like proteins were the elusive energy coupling factor that interacts with various substrate specific proteins. A large-scale bioinformatics analysis was performed to search for additional vitamin-binding proteins and putative energy coupling factors (108). A total of 365 genomes were search ...
... the BioM like proteins were the elusive energy coupling factor that interacts with various substrate specific proteins. A large-scale bioinformatics analysis was performed to search for additional vitamin-binding proteins and putative energy coupling factors (108). A total of 365 genomes were search ...
msb145697-sup-0001-Supp_Info
... various steps of the experimental flow. The R-sector includes most of the r-proteins which together form one complex, ribosome. Similar behaviors of this large group of proteins in terms of noise could cause the observed large variation for the R-sector. ...
... various steps of the experimental flow. The R-sector includes most of the r-proteins which together form one complex, ribosome. Similar behaviors of this large group of proteins in terms of noise could cause the observed large variation for the R-sector. ...
Characterisation of hexon and fibre genes of a novel strain of
... in a microtitre plate containing a confluent monolayer of A549 cells. Aliquots (25 ml) of 100 tissue culture infectious doses of virus (100TCID50) were incubated with 25 ml of serially diluted type specific antisera at 37˚C for 60 minutes and then inoculated into A549 cells. Viral growth was inhibit ...
... in a microtitre plate containing a confluent monolayer of A549 cells. Aliquots (25 ml) of 100 tissue culture infectious doses of virus (100TCID50) were incubated with 25 ml of serially diluted type specific antisera at 37˚C for 60 minutes and then inoculated into A549 cells. Viral growth was inhibit ...
- Cal State LA - Instructional Web Server
... Expression of Nearby Genes Kendra Baughman York Marahrens’ Lab UCLA ...
... Expression of Nearby Genes Kendra Baughman York Marahrens’ Lab UCLA ...
Recombinant DNA Technology
... compatible host cells. In order to be propagated, the recombinant DNA molecule (insert DNA joined to vector DNA) must be introduced into a compatible host cell where it can replicate. The direct uptake of foreign DNA by a host cell is called genetic transformation (or transformation). Recombinant DN ...
... compatible host cells. In order to be propagated, the recombinant DNA molecule (insert DNA joined to vector DNA) must be introduced into a compatible host cell where it can replicate. The direct uptake of foreign DNA by a host cell is called genetic transformation (or transformation). Recombinant DN ...
Structure and Physiological significance of lipid
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
GenomeSequencing_ver3_20040929
... Raw sequence: unassembled sequence reads produced from sequencing of inserts from individual recombinant clones of a genomic DNA library. Finished sequence: complete sequence of a genome with no gaps and an accuracy of > 99.9%. Genome coverage: average number of times a nucleotide is represented by ...
... Raw sequence: unassembled sequence reads produced from sequencing of inserts from individual recombinant clones of a genomic DNA library. Finished sequence: complete sequence of a genome with no gaps and an accuracy of > 99.9%. Genome coverage: average number of times a nucleotide is represented by ...
Jamieson
... used as a source of nitrogen and sulphur under starvation conditions.36,102 While many studies have shown that menadione can lead to the production of superoxide anions, via redox-cycling there is still some doubt as to the precise mode of toxicity of menadione. Cu/ ZnSOD-deficient yeast mutants are ...
... used as a source of nitrogen and sulphur under starvation conditions.36,102 While many studies have shown that menadione can lead to the production of superoxide anions, via redox-cycling there is still some doubt as to the precise mode of toxicity of menadione. Cu/ ZnSOD-deficient yeast mutants are ...
Article Lateral Gene Transfer and Gene
... mitochondria, and phylogenetic analysis of its two key components, ATP sulfurylase (AS) and adenosine-50 -phosphosulfate kinase (APSK), suggests that the corresponding genes were acquired laterally from a bacterial source. Interestingly, in Entamoeba’s relative M. balamuthi, PFO and hydrogenase are ...
... mitochondria, and phylogenetic analysis of its two key components, ATP sulfurylase (AS) and adenosine-50 -phosphosulfate kinase (APSK), suggests that the corresponding genes were acquired laterally from a bacterial source. Interestingly, in Entamoeba’s relative M. balamuthi, PFO and hydrogenase are ...
Agrobacterium tumefaciens T DNA mediated transformation of
... copy insertion. Single copy insertions are the preferred outcome for genetic analysis and molecular rescue of the flanking E. festucae sequences. However, it is also important that the mutant phenotype is linked to the T-DNA insertion. Genetic analysis of a large set of T-DNA induced mutants from Cry ...
... copy insertion. Single copy insertions are the preferred outcome for genetic analysis and molecular rescue of the flanking E. festucae sequences. However, it is also important that the mutant phenotype is linked to the T-DNA insertion. Genetic analysis of a large set of T-DNA induced mutants from Cry ...
Our work was originally motivated my collaboration with Drs
... to 4,200 distinct tri-peptides (not counting mirror images, e.g., ABC is the same as CBA). For quadripeptides, the number of distinct types is approximately 20 times larger. We are looking for differential binding profiles by using specially designed phage probes. A DNA sequence corresponding to a ...
... to 4,200 distinct tri-peptides (not counting mirror images, e.g., ABC is the same as CBA). For quadripeptides, the number of distinct types is approximately 20 times larger. We are looking for differential binding profiles by using specially designed phage probes. A DNA sequence corresponding to a ...
... same tRNA can be used to translate more than one codon. The diagram to the right may be helpful. Choice C: Discuss the mechanism by which some DNA polymerases remove incorrectly incorporated bases. What is the consequence of lack of this function in a polymerase and how does it affect the treatment ...
1 laboratory 9 construction of a fusion protein
... around for a while but would not permanently affect the cell culture, if we were to keep the cells growing for many generations. In transient transfections, the DNA is maintained extrachromasomally (meaning as a separate piece of DNA in the nucleus). Since the DNA does not replicate or attach to th ...
... around for a while but would not permanently affect the cell culture, if we were to keep the cells growing for many generations. In transient transfections, the DNA is maintained extrachromasomally (meaning as a separate piece of DNA in the nucleus). Since the DNA does not replicate or attach to th ...
Purine Oct 20 - LSU School of Medicine
... reductase gene • Also used for treatment of rheumatoid arthritis and psoriasis at lower doses, though site of action is not through DHFR but inhibition of salvage pathways that lead to increased adenosine that inhibits T cell activation. ...
... reductase gene • Also used for treatment of rheumatoid arthritis and psoriasis at lower doses, though site of action is not through DHFR but inhibition of salvage pathways that lead to increased adenosine that inhibits T cell activation. ...
Pantoea ananatis competition Divine Y. Shyntum, Jacques Theron,
... The T6SSs have been implicated in a variety of functions ranging from biofilm formation to host-cell invasion, cytotoxicity and survival in macrophages (Aschtgen et al. 2008; Cascales 2008; Schwarz et al. 2010a). However, most studies of the T6SS have focused on its role in pathogenesis and host int ...
... The T6SSs have been implicated in a variety of functions ranging from biofilm formation to host-cell invasion, cytotoxicity and survival in macrophages (Aschtgen et al. 2008; Cascales 2008; Schwarz et al. 2010a). However, most studies of the T6SS have focused on its role in pathogenesis and host int ...
Emerging real-time PCR applications.
... of markers that are sensitive to the disease state. Microarray screening can be a first step in such process, but the number of markers should be reduced and the test preferably converted to realtime PCR to improve dynamic range, enhance sensitivity and reduce costs. One such test is the Oncotype Dx ...
... of markers that are sensitive to the disease state. Microarray screening can be a first step in such process, but the number of markers should be reduced and the test preferably converted to realtime PCR to improve dynamic range, enhance sensitivity and reduce costs. One such test is the Oncotype Dx ...
Lactobacilli carry cryptic genes encoding peptidase
... For cloning of pepQ, the upstream and coding regions of the pepQ gene were cloned in pJDC9 as a PCR product synthesized with the primer 5' TTGTGCAATCGCTTACAG 3' (p290), designed according to the upstream region of Lb. delbrueckii subsp. l a d s pepQ (Stucky et al., 1995) and with the Lb. delbrueckii ...
... For cloning of pepQ, the upstream and coding regions of the pepQ gene were cloned in pJDC9 as a PCR product synthesized with the primer 5' TTGTGCAATCGCTTACAG 3' (p290), designed according to the upstream region of Lb. delbrueckii subsp. l a d s pepQ (Stucky et al., 1995) and with the Lb. delbrueckii ...
Small Nucleolar RNAs and Pre
... generate the mature SSU rRNA and a precursor containing the 5.8S and LSU rRNAs. Finally, cleavage in ITS2 and the 39 ETS generate mature 5.8S and LSU rRNAs. In addition to these cleavage reactions, rRNAs also undergo extensive modifications, the most common of which are 29-O-ribose methylation and p ...
... generate the mature SSU rRNA and a precursor containing the 5.8S and LSU rRNAs. Finally, cleavage in ITS2 and the 39 ETS generate mature 5.8S and LSU rRNAs. In addition to these cleavage reactions, rRNAs also undergo extensive modifications, the most common of which are 29-O-ribose methylation and p ...