Directed Evolution of ATP Binding Proteins from a Zinc Finger
... pool (see Figure S1); thus, the conservation of these positions in the evolved pool is the result of some selective advantage. Apart from these invariant positions, the random loop sequences appear to be quite diverse, and are therefore of independent origin in the starting pool. The mutations in th ...
... pool (see Figure S1); thus, the conservation of these positions in the evolved pool is the result of some selective advantage. Apart from these invariant positions, the random loop sequences appear to be quite diverse, and are therefore of independent origin in the starting pool. The mutations in th ...
Phylogeny of Geminiviruses - Journal of General Virology
... whitefly- and leafhopper-transmitted geminiviruses (Thomas et al., 1986). These observations, together with the fact that coat protein is the only protein that has been purified from most geminiviruses, limits the usefulness of a serological approach in a comparative study. We have overcome these pr ...
... whitefly- and leafhopper-transmitted geminiviruses (Thomas et al., 1986). These observations, together with the fact that coat protein is the only protein that has been purified from most geminiviruses, limits the usefulness of a serological approach in a comparative study. We have overcome these pr ...
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... All these past attempts to create novel-coloured flowers have focused on the manipulation of the flavonoid pigmentation pathways that exist in flowers (Elomaa and Holton, 1994; Forkmann and Martens, 2001). To create black flowers, for example, researchers tried to increase the concentrations of fl ...
... All these past attempts to create novel-coloured flowers have focused on the manipulation of the flavonoid pigmentation pathways that exist in flowers (Elomaa and Holton, 1994; Forkmann and Martens, 2001). To create black flowers, for example, researchers tried to increase the concentrations of fl ...
trypsin inhibitor and castor-bean (Ricinus communis) storage protein
... tested by the Moore-Goodman test (Moore & Goodman, 1977), which is based on a count of minimum mutation distances (Fitch & Margoliash, 1967) between two sequences to be compared. The minimum mutation distance value for this 73alignment (residues 1-74, with two gaps) is 76, giving a 'significance lev ...
... tested by the Moore-Goodman test (Moore & Goodman, 1977), which is based on a count of minimum mutation distances (Fitch & Margoliash, 1967) between two sequences to be compared. The minimum mutation distance value for this 73alignment (residues 1-74, with two gaps) is 76, giving a 'significance lev ...
... it has been shown that the addition of tryptophan results in further inhibition. Half-maximum inhibition was observed at about 10w5M tryptophan and the maximum effect was given by about 4 x lo-5M tryptophan. In terms of total DAHP synthetase activity, the tryptophan inhibited portion is about 11%. A ...
Hsp90
... The physical folding code How is the 3D structure determined by the physicochemical properties encoded in the amino acid sequence? The folding mechanism How can proteins fold so fast even with so many possible conformations? Predicting protein structure using ...
... The physical folding code How is the 3D structure determined by the physicochemical properties encoded in the amino acid sequence? The folding mechanism How can proteins fold so fast even with so many possible conformations? Predicting protein structure using ...
Enhancer timing of Hox gene expression: deletion of
... by retinoic acid (RA) prematurely (Bel-Vialar et al., 2000). These studies suggest that in the initiation phase, a repressive regulatory mechanism is involved in regulating the correct temporal activation of Hox genes by modulating chromatin structure. Therefore, Hox genes must release sequentially ...
... by retinoic acid (RA) prematurely (Bel-Vialar et al., 2000). These studies suggest that in the initiation phase, a repressive regulatory mechanism is involved in regulating the correct temporal activation of Hox genes by modulating chromatin structure. Therefore, Hox genes must release sequentially ...
1 Irreversible Enzyme Inhibition Affinity labels for studying enzyme
... increases the affinity of other binding sites) nH < 1 negative cooperativity nH = n (where n = 4) complete cooperativity for Hb ...
... increases the affinity of other binding sites) nH < 1 negative cooperativity nH = n (where n = 4) complete cooperativity for Hb ...
Getting a grip on how DNA polymerases function
... multiple DNA polymerases have been identified in prokaryotes and eukaryotes, including the recent discovery of several error-prone DNA polymerases2. Based on primary sequence similarity, these DNA polymerases can be categorized into families (Table 1). The most extensively studied polymerases includ ...
... multiple DNA polymerases have been identified in prokaryotes and eukaryotes, including the recent discovery of several error-prone DNA polymerases2. Based on primary sequence similarity, these DNA polymerases can be categorized into families (Table 1). The most extensively studied polymerases includ ...
Protein Structure Prediction and Structural Genomics
... identity and become the most substantial origin of errors in comparative models. In addition, when a model is based on an almost insignificant alignment to a known structure, it may also have an entirely incorrect fold. Accuracies of the best model building methods are relatively similar when used o ...
... identity and become the most substantial origin of errors in comparative models. In addition, when a model is based on an almost insignificant alignment to a known structure, it may also have an entirely incorrect fold. Accuracies of the best model building methods are relatively similar when used o ...
- Wiley Online Library
... that B. subtilis has two distinct nitrate reductases, one is assimilatory and the other respiratory [1,3,13]. In retrospect, the nitrate reductase reported above should be the respiratory one encoded by narGHJI since the enzyme was shown later to be induced under anaerobiosis and not under the contr ...
... that B. subtilis has two distinct nitrate reductases, one is assimilatory and the other respiratory [1,3,13]. In retrospect, the nitrate reductase reported above should be the respiratory one encoded by narGHJI since the enzyme was shown later to be induced under anaerobiosis and not under the contr ...
Alfalfa Cyclins: Differential Expression during the Cell
... nucleotidesto the redundant oligonucleotide probe of the cyclin B class (see above). However, at the amino acid levei, only five of the encoded seven amino acids fit to the highly conserved cyclin B box (the encoded sequence reads I-L-V-D-C-L-L instead of I-L-V-D-W-L-V).The clone contains a nontrans ...
... nucleotidesto the redundant oligonucleotide probe of the cyclin B class (see above). However, at the amino acid levei, only five of the encoded seven amino acids fit to the highly conserved cyclin B box (the encoded sequence reads I-L-V-D-C-L-L instead of I-L-V-D-W-L-V).The clone contains a nontrans ...
IS Elements
... the human genome. SINE families are the Alu, MIR, and Ther2/MIR3 elements. SINEs are usually less than 400 base pairs long and do not encode proteins. The reverse transcriptase required for SINE transposition is provided by a LINE-type ...
... the human genome. SINE families are the Alu, MIR, and Ther2/MIR3 elements. SINEs are usually less than 400 base pairs long and do not encode proteins. The reverse transcriptase required for SINE transposition is provided by a LINE-type ...
c-MET - Siapec
... nuclear regulatory protein that interacts with specific genes involved in cellular regulation, invasion and progression expression of MTA-1 gene was increased four-times in cerbB2/neu stable transfectants cells compared to untransfected cells MTA-1 is overexpressed in a variety of human cancer ...
... nuclear regulatory protein that interacts with specific genes involved in cellular regulation, invasion and progression expression of MTA-1 gene was increased four-times in cerbB2/neu stable transfectants cells compared to untransfected cells MTA-1 is overexpressed in a variety of human cancer ...
Recombinant DNA Technology
... provided new means to determine the functions of proteins and of individual domains within proteins, revealing a host of unexpected relationships between them. By making available large amounts of any protein, they have shown the way to efficient mass production of protein hormones and vaccines. Fin ...
... provided new means to determine the functions of proteins and of individual domains within proteins, revealing a host of unexpected relationships between them. By making available large amounts of any protein, they have shown the way to efficient mass production of protein hormones and vaccines. Fin ...
DNA Replication
... single high density band - all DNA molecules contained the "heavy" nitrogen. ･DNA taken from the two generations after the switch contained an intermediate-density band - DNA contained a "heavy" DNA strand from the parent and a complementary "light" DNA strand. ･Density results from generation 3, di ...
... single high density band - all DNA molecules contained the "heavy" nitrogen. ･DNA taken from the two generations after the switch contained an intermediate-density band - DNA contained a "heavy" DNA strand from the parent and a complementary "light" DNA strand. ･Density results from generation 3, di ...
Kuever et al_final.p
... from a freshwater ditch and is of interest because it can grow with a large variety of organic substrates, in particular several aromatic compounds, short-chain and mediumchain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow autotrophical ...
... from a freshwater ditch and is of interest because it can grow with a large variety of organic substrates, in particular several aromatic compounds, short-chain and mediumchain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow autotrophical ...
Expression and purification of proteins using Strep
... to its chemically balanced amino acid composition (8 amino acids, WSHPQFEK) which can be fused to the protein as either N- or C-terminal tag. A two amino acid spacer (SerAla) between the protein and the tag promotes the accessibility of the tag. The further improved Twin-Strep-tag® is a sequential a ...
... to its chemically balanced amino acid composition (8 amino acids, WSHPQFEK) which can be fused to the protein as either N- or C-terminal tag. A two amino acid spacer (SerAla) between the protein and the tag promotes the accessibility of the tag. The further improved Twin-Strep-tag® is a sequential a ...