7.06 Cell Biology EXAM #3
... For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume that each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found. Also assume that the only targeting sequences possess ...
... For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume that each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found. Also assume that the only targeting sequences possess ...
Predictable Alteration of Sequence Recognition by RNA
... OTP82, and using the modified proteins to attempt to complement the respective mutants, we demonstrate that we can predictably alter the specificity of these factors in vivo. ...
... OTP82, and using the modified proteins to attempt to complement the respective mutants, we demonstrate that we can predictably alter the specificity of these factors in vivo. ...
Continued..
... immunodeficiency virus was first identified as an aspartyl protease because a characteristic sequence motif for such proteases was recognized in its primary structure. ...
... immunodeficiency virus was first identified as an aspartyl protease because a characteristic sequence motif for such proteases was recognized in its primary structure. ...
Regulation of Galactoside Transport by the PTS
... Among four proteins (LacY, MelB, MalK, and GlpK) that interact with IIAGlc, MelB is considered to be a little different one, since some of the conserved residues in REGION II are absent. In fact, our recent experiments demonstrated that deletion of Arg-215 to Leu-220, which corresponds to the latter ...
... Among four proteins (LacY, MelB, MalK, and GlpK) that interact with IIAGlc, MelB is considered to be a little different one, since some of the conserved residues in REGION II are absent. In fact, our recent experiments demonstrated that deletion of Arg-215 to Leu-220, which corresponds to the latter ...
Multiple Molecular Mechanisms Cause Reproductive Isolation
... bayanus nuclear gene, AEP2, and S. cerevisiae mitochondria causes interspecific F2 hybrid sterility [14]. To examine whether nuclearmitochondrial incompatibility represents a general mechanism of reproductive isolation in yeast, a systematic screen for such genes was conducted in three closely relat ...
... bayanus nuclear gene, AEP2, and S. cerevisiae mitochondria causes interspecific F2 hybrid sterility [14]. To examine whether nuclearmitochondrial incompatibility represents a general mechanism of reproductive isolation in yeast, a systematic screen for such genes was conducted in three closely relat ...
A Mutation in the Anticodon of a Single tRNA Is
... were more rounded with shortened petioles, but it is distinct in that it appears to retain sensitivity to ethylene and the mutation is homozygous lethal. Surprisingly, positional cloning on the basis of 2,4-D resistance revealed that the mutation linked to the observed phenotypes was not found in an ...
... were more rounded with shortened petioles, but it is distinct in that it appears to retain sensitivity to ethylene and the mutation is homozygous lethal. Surprisingly, positional cloning on the basis of 2,4-D resistance revealed that the mutation linked to the observed phenotypes was not found in an ...
The Age of the Common Ancestor of Eukaryotes and
... average over all possible combinations. It was shown that the asymptotic bias of the estimate cx can be significantly reduced if an appropriate weight function is chosen for combining the estimates of three-sequence sets (Gu 1996). The computer program, which was originally developed for nucleotide ...
... average over all possible combinations. It was shown that the asymptotic bias of the estimate cx can be significantly reduced if an appropriate weight function is chosen for combining the estimates of three-sequence sets (Gu 1996). The computer program, which was originally developed for nucleotide ...
Antigen Binding and Idiotype Analysis of Antibodies Obtained
... cells along with one of the three dominant L chain genes from PC-binding antibodies (Table 1, cell lines 1-3). As expected, the antibodies produced by all combinations of electroporated H and L chain genes expressed K, #~-allotype, and VHI-Id markers. The homologous T15 cell line, which resulted fro ...
... cells along with one of the three dominant L chain genes from PC-binding antibodies (Table 1, cell lines 1-3). As expected, the antibodies produced by all combinations of electroporated H and L chain genes expressed K, #~-allotype, and VHI-Id markers. The homologous T15 cell line, which resulted fro ...
A Protein Extract from Chicken Reduces Plasma Homocysteine in Rats
... Hypolipidemic effects of processed proteins or peptides have been reported in numerous different animal models [1–7]. These effects are most likely due to the amino acid composition or specific peptides in the different protein sources [1,8–10]. Recently, we have shown that a water-soluble protein e ...
... Hypolipidemic effects of processed proteins or peptides have been reported in numerous different animal models [1–7]. These effects are most likely due to the amino acid composition or specific peptides in the different protein sources [1,8–10]. Recently, we have shown that a water-soluble protein e ...
Sequence-Specific Resonance Assignments of the `H
... as the simian immunodeficiency virus (SIV), the bovine immunodeficiency virus (BIV), the feline immunodeficiency virus (FIV), the sheep visna virus (VILV), the caprine arthritis encephalitisvirus (CAEV), and the ovine lentivirus (OMVV). Retroviruses use a complex machinery of RNA-binding proteins to ...
... as the simian immunodeficiency virus (SIV), the bovine immunodeficiency virus (BIV), the feline immunodeficiency virus (FIV), the sheep visna virus (VILV), the caprine arthritis encephalitisvirus (CAEV), and the ovine lentivirus (OMVV). Retroviruses use a complex machinery of RNA-binding proteins to ...
Analysis of expressed sequence tags from Cryptomeria japonica
... Summary Cryptomeria japonica D. Don is one of the most important forest trees in Japan, but more than 10% of the Japanese population is allergic to its pollen. We constructed a cDNA library derived from pollen grains of C. japonica and performed an analysis of expressed sequence tags (ESTs). We obta ...
... Summary Cryptomeria japonica D. Don is one of the most important forest trees in Japan, but more than 10% of the Japanese population is allergic to its pollen. We constructed a cDNA library derived from pollen grains of C. japonica and performed an analysis of expressed sequence tags (ESTs). We obta ...
PDF - Bentham Open
... E. sibiricum 255-15 Cell Culture and Cell Lysis E. sibiricum 255-15 cell pellets were obtained from the Department of the Food Science at North Carolina State University. All cells were cultured in tryptic soy broth (TSB, Difco, BD Diagnostics Systems, Franklin Lakes, NJ) with 7% yeast extract (Difc ...
... E. sibiricum 255-15 Cell Culture and Cell Lysis E. sibiricum 255-15 cell pellets were obtained from the Department of the Food Science at North Carolina State University. All cells were cultured in tryptic soy broth (TSB, Difco, BD Diagnostics Systems, Franklin Lakes, NJ) with 7% yeast extract (Difc ...
Spatial restriction of AChR gene expression to
... 24 nucleotides are not likely to be critical for localizing delta subunit and hGH mRNAs to synaptic sites. Thus, our data indicate that myofiber nuclei that are positioned close to the synaptic site transcribe the endogenous delta subunit gene at a higher rate than nuclei elsewhere in the myofiber. ...
... 24 nucleotides are not likely to be critical for localizing delta subunit and hGH mRNAs to synaptic sites. Thus, our data indicate that myofiber nuclei that are positioned close to the synaptic site transcribe the endogenous delta subunit gene at a higher rate than nuclei elsewhere in the myofiber. ...
Mass spectrometry and the search for moonlighting proteins
... functions. In some cases, the two functions are very different, as in PHGPx (glutathione peroxidase), a soluble enzyme that is also a sperm structural protein (Ursini et al., 1999). In other proteins, the two functions appear to be more closely related, such as the PMS2 DNA mismatch repair enzyme th ...
... functions. In some cases, the two functions are very different, as in PHGPx (glutathione peroxidase), a soluble enzyme that is also a sperm structural protein (Ursini et al., 1999). In other proteins, the two functions appear to be more closely related, such as the PMS2 DNA mismatch repair enzyme th ...
Ribosomes: Cashing in on crystals
... density between the bound and free ribosome crystals. The overall architecture of the 70S ribosome seen here is similar to that observed previously by cryo-electron microscopy. Furthermore, structural features seen in the 30S and 50S electron density maps are also observed, such as the long rRNA hel ...
... density between the bound and free ribosome crystals. The overall architecture of the 70S ribosome seen here is similar to that observed previously by cryo-electron microscopy. Furthermore, structural features seen in the 30S and 50S electron density maps are also observed, such as the long rRNA hel ...
Gene Section TGFB1 (transforming growth factor, beta 1)
... et al., 1994) and Ras/Raf/ERK (Matsuzaki, 2011). In the SMAD-dependent pathways, the receptor complex (or directly the type I receptor) phosphorylates receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD3, SMAD5 and SMAD8) which can now bind the cooperative SMAD (co-SMAD) SMAD4. SMAD6 and SMAD7 hav ...
... et al., 1994) and Ras/Raf/ERK (Matsuzaki, 2011). In the SMAD-dependent pathways, the receptor complex (or directly the type I receptor) phosphorylates receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD3, SMAD5 and SMAD8) which can now bind the cooperative SMAD (co-SMAD) SMAD4. SMAD6 and SMAD7 hav ...
A Few Good Domains
... understood, it appears to involve a highly conserved set of proteins called SNAREs (Soluble NSF Attachment protein [SNAP] Receptors). SNARE proteins are believed to mediate most, if not all, cellular membrane fusion events. Most SNAREs are Cterminally anchored integral membrane proteins capable of e ...
... understood, it appears to involve a highly conserved set of proteins called SNAREs (Soluble NSF Attachment protein [SNAP] Receptors). SNARE proteins are believed to mediate most, if not all, cellular membrane fusion events. Most SNAREs are Cterminally anchored integral membrane proteins capable of e ...
The identification of transcription factors expressed in the notochord
... Ciona intestinalis at various stages of embryonic development. Four of these transcription factors, Fos-a, NFAT5, AFF and Klf15, have not been directly associated with the notochord in previous studies, while the others, including Spalt-like-a, Lmx-like, and STAT5/6-b, display evolutionarily conserv ...
... Ciona intestinalis at various stages of embryonic development. Four of these transcription factors, Fos-a, NFAT5, AFF and Klf15, have not been directly associated with the notochord in previous studies, while the others, including Spalt-like-a, Lmx-like, and STAT5/6-b, display evolutionarily conserv ...
Protein Annotation & Advanced Insilico Based Mutation Hotspot Oryza sativa *
... RASMOL. The major step of the research is to evaluate the effect of the substitution mutation at the hot spot site on the stability, functionality disease occurrence and tolerability of the protein. These parameters were calculated using various insilico tools. The final summary of the work is that ...
... RASMOL. The major step of the research is to evaluate the effect of the substitution mutation at the hot spot site on the stability, functionality disease occurrence and tolerability of the protein. These parameters were calculated using various insilico tools. The final summary of the work is that ...
PDF file
... The effects of C-terminal truncation on phosphatase activity measured with the small RNA and artificial protein substrates employed in these studies may not reflect the properties of PIR1 with physiologic substrates. Moreover, these findings do not eliminate a role for the noncatalytic domain in mod ...
... The effects of C-terminal truncation on phosphatase activity measured with the small RNA and artificial protein substrates employed in these studies may not reflect the properties of PIR1 with physiologic substrates. Moreover, these findings do not eliminate a role for the noncatalytic domain in mod ...
Improved insights into protein thermal stability: from the
... where k is the Boltzmann constant. These probabilities can be estimated in terms of the number of occurrences n(c, s), n(c) n(s) and n in a structure dataset (see [5] for details). The potentials so defined conserve the memory of the protein dataset from which they are extracted. It is generally adm ...
... where k is the Boltzmann constant. These probabilities can be estimated in terms of the number of occurrences n(c, s), n(c) n(s) and n in a structure dataset (see [5] for details). The potentials so defined conserve the memory of the protein dataset from which they are extracted. It is generally adm ...
Development of Biocatalysts for Production of Fine Chemicals
... Biocatalysis has matured into the standard technology for synthesizing industrially-important chemicals such as pharmaceuticals and agrochemicals. The principal advantage of biocatalysts is their ability to catalyze reactions with high specificity (often enantio- or regio-selectively). Furthermore, ...
... Biocatalysis has matured into the standard technology for synthesizing industrially-important chemicals such as pharmaceuticals and agrochemicals. The principal advantage of biocatalysts is their ability to catalyze reactions with high specificity (often enantio- or regio-selectively). Furthermore, ...
during Epstein-Barr Virus Reactivation Transcription by Lytic
... This work was supported by National Natural Science Foundation of China (Grant 30771981), National Basic Research Program of China (973 Program, Grant 2006CB504305), and National High-Tech Research and Development Plan (863 Program, Grant 2006AA02A245). ...
... This work was supported by National Natural Science Foundation of China (Grant 30771981), National Basic Research Program of China (973 Program, Grant 2006CB504305), and National High-Tech Research and Development Plan (863 Program, Grant 2006AA02A245). ...